Mercurial > repos > xuebing > sharplabtool
view tools/fastq/fastq_groomer.py @ 2:c2a356708570
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author | xuebing |
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date | Fri, 09 Mar 2012 19:45:42 -0500 |
parents | 9071e359b9a3 |
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#Dan Blankenberg import sys from galaxy_utils.sequence.fastq import fastqReader, fastqVerboseErrorReader, fastqAggregator, fastqWriter def main(): input_filename = sys.argv[1] input_type = sys.argv[2] output_filename = sys.argv[3] output_type = sys.argv[4] force_quality_encoding = sys.argv[5] summarize_input = sys.argv[6] == 'summarize_input' if force_quality_encoding == 'None': force_quality_encoding = None aggregator = fastqAggregator() out = fastqWriter( open( output_filename, 'wb' ), format = output_type, force_quality_encoding = force_quality_encoding ) read_count = None if summarize_input: reader = fastqVerboseErrorReader else: reader = fastqReader for read_count, fastq_read in enumerate( reader( open( input_filename ), format = input_type, apply_galaxy_conventions = True ) ): if summarize_input: aggregator.consume_read( fastq_read ) out.write( fastq_read ) out.close() if read_count is not None: print "Groomed %i %s reads into %s reads." % ( read_count + 1, input_type, output_type ) if input_type != output_type and 'solexa' in [ input_type, output_type ]: print "Converted between Solexa and PHRED scores." if summarize_input: print "Based upon quality and sequence, the input data is valid for: %s" % ( ", ".join( aggregator.get_valid_formats() ) or "None" ) ascii_range = aggregator.get_ascii_range() decimal_range = aggregator.get_decimal_range() print "Input ASCII range: %s(%i) - %s(%i)" % ( repr( ascii_range[0] ), ord( ascii_range[0] ), repr( ascii_range[1] ), ord( ascii_range[1] ) ) #print using repr, since \x00 (null) causes info truncation in galaxy when printed print "Input decimal range: %i - %i" % ( decimal_range[0], decimal_range[1] ) else: print "No valid FASTQ reads were provided." if __name__ == "__main__": main()