Mercurial > repos > xuebing > spatial_proximity
diff spatial_proximity.py @ 2:e68d35c7d3eb default tip
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author | xuebing |
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date | Sat, 31 Mar 2012 14:09:14 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/spatial_proximity.py Sat Mar 31 14:09:14 2012 -0400 @@ -0,0 +1,36 @@ + +import os,sys + +file1 = sys.argv[1] +file2 = sys.argv[2] +genome = sys.argv[3] +outplot = sys.argv[4] +outlog = sys.argv[5] +outbed = sys.argv[6] + +strandness = '' +if len(sys.argv) > 7: + strandness = sys.argv[7] + +# real distance +cmd = 'closestBed -a '+file1+' -b '+file2 + ' '+strandness + ' -d -t first > '+outbed +os.system(cmd) +# shuffle +cmd = 'shuffleBed -chrom -g '+genome+' -i '+file1+'> shuffled.bed' +os.system(cmd) +# shuffled distance +cmd = 'closestBed -a shuffled.bed -b '+file2 + ' '+strandness + ' -d -t first > shuffled.dist' +os.system(cmd) + + +# test in R +r = open('tmp.r','w') +r.write("options(warn=-1)\n") +r.write("source('/Users/xuebing/galaxy-dist/tools/mytools/cdf.r')\n") +r.write("x = read.table('"+outbed+"',sep='\t')\n") +r.write("y = read.table('shuffled.dist',sep='\t')\n") +r.write("pdf('"+outplot+"')\n") +r.write("mycdf(list(log10(1+x[,ncol(x)]),log10(1+y[,ncol(y)])),'spatial distance',c('real','shuffled'),'topleft','log10 distance','')\n") +r.write("dev.off()\n") +r.close() +os.system("R --vanilla < tmp.r >"+outlog)