Mercurial > repos > yating-l > hubarchivecreator
comparison hubArchiveCreator.py @ 0:f493979f1408 draft default tip
planemo upload for repository https://github.com/Yating-L/hubarchivecreator-test commit 48b59e91e2dcc2e97735ee35d587960cbfbce932-dirty
| author | yating-l |
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| date | Wed, 21 Dec 2016 12:13:04 -0500 |
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| -1:000000000000 | 0:f493979f1408 |
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| 1 #!/usr/bin/python | |
| 2 # -*- coding: utf8 -*- | |
| 3 | |
| 4 """ | |
| 5 This Galaxy tool permits to prepare your files to be ready for | |
| 6 Assembly Hub visualization. | |
| 7 Program test arguments: | |
| 8 hubArchiveCreator.py -g test-data/augustusDbia3.gff3 -f test-data/dbia3.fa -d . -u ./tools -o output.html | |
| 9 """ | |
| 10 | |
| 11 import argparse | |
| 12 import collections | |
| 13 import json | |
| 14 import logging | |
| 15 import os | |
| 16 import sys | |
| 17 | |
| 18 # Internal dependencies | |
| 19 from Bam import Bam | |
| 20 from BedSimpleRepeats import BedSimpleRepeats | |
| 21 from BedSpliceJunctions import BedSpliceJunctions | |
| 22 from Bed import Bed | |
| 23 from BigWig import BigWig | |
| 24 from util.Fasta import Fasta | |
| 25 from util.Filters import TraceBackFormatter | |
| 26 from Gff3 import Gff3 | |
| 27 from Gtf import Gtf | |
| 28 from Psl import Psl | |
| 29 from TrackHub import TrackHub | |
| 30 | |
| 31 # TODO: Verify each subprocessed dependency is accessible [gff3ToGenePred, genePredToBed, twoBitInfo, faToTwoBit, bedToBigBed, sort | |
| 32 | |
| 33 | |
| 34 def main(argv): | |
| 35 # Command Line parsing init | |
| 36 parser = argparse.ArgumentParser(description='Create a foo.txt inside the given folder.') | |
| 37 | |
| 38 # Reference genome mandatory | |
| 39 parser.add_argument('-f', '--fasta', help='Fasta file of the reference genome') | |
| 40 | |
| 41 # GFF3 Management | |
| 42 parser.add_argument('--gff3', action='append', help='GFF3 format') | |
| 43 | |
| 44 # GTF Management | |
| 45 parser.add_argument('--gtf', action='append', help='GTF format') | |
| 46 | |
| 47 # Bed4+12 (TrfBig) | |
| 48 parser.add_argument('--bedSimpleRepeats', action='append', help='Bed4+12 format, using simpleRepeats.as') | |
| 49 | |
| 50 # Bed12+1 (regtools) | |
| 51 parser.add_argument('--bedSpliceJunctions', action='append', help='Bed12+1 format, using spliceJunctions.as') | |
| 52 | |
| 53 # Generic Bed (Blastx transformed to bed) | |
| 54 parser.add_argument('--bed', action='append', help='Bed generic format') | |
| 55 | |
| 56 # BigWig Management | |
| 57 parser.add_argument('--bigwig', action='append', help='BigWig format') | |
| 58 | |
| 59 # Bam Management | |
| 60 parser.add_argument('--bam', action='append', help='Bam format') | |
| 61 | |
| 62 # Psl Management | |
| 63 parser.add_argument('--psl', action='append', help='Psl format') | |
| 64 | |
| 65 # TODO: Check if the running directory can have issues if we run the tool outside | |
| 66 parser.add_argument('-d', '--directory', | |
| 67 help='Running tool directory, where to find the templates. Default is running directory') | |
| 68 parser.add_argument('-u', '--ucsc_tools_path', | |
| 69 help='Directory where to find the executables needed to run this tool') | |
| 70 parser.add_argument('-e', '--extra_files_path', | |
| 71 help='Name, in galaxy, of the output folder. Where you would want to build the Track Hub Archive') | |
| 72 parser.add_argument('-o', '--output', help='Name of the HTML summarizing the content of the Track Hub Archive') | |
| 73 | |
| 74 parser.add_argument('-j', '--data_json', help='Json containing the metadata of the inputs') | |
| 75 | |
| 76 parser.add_argument('--user_email', help='Email of the user who launched the Hub Archive Creation') | |
| 77 | |
| 78 parser.add_argument('--genome_name', help='UCSC Genome Browser assembly ID') | |
| 79 | |
| 80 parser.add_argument('--debug_mode', action='store_true', help='Allow more details about the errors') | |
| 81 | |
| 82 # Begin init variables | |
| 83 | |
| 84 toolDirectory = '.' | |
| 85 extra_files_path = '.' | |
| 86 | |
| 87 # Get the args passed in parameter | |
| 88 args = parser.parse_args() | |
| 89 | |
| 90 extra_files_path = args.extra_files_path | |
| 91 toolDirectory = args.directory | |
| 92 | |
| 93 #### Logging management #### | |
| 94 # If we are in Debug mode, also print in stdout the debug dump | |
| 95 | |
| 96 configure_logger(extra_files_path=extra_files_path, debug=args.debug_mode) | |
| 97 | |
| 98 #### END Logging management #### | |
| 99 | |
| 100 array_inputs_reference_genome = json.loads(args.fasta) | |
| 101 | |
| 102 # TODO: Replace these with the object Fasta | |
| 103 input_fasta_file = array_inputs_reference_genome["false_path"] | |
| 104 input_fasta_file_name = sanitize_name_input(array_inputs_reference_genome["name"]) | |
| 105 genome_name = sanitize_name_input(args.genome_name) | |
| 106 | |
| 107 reference_genome = Fasta(input_fasta_file, | |
| 108 input_fasta_file_name, genome_name) | |
| 109 | |
| 110 user_email = args.user_email | |
| 111 | |
| 112 | |
| 113 # TODO: Use a class to have a better management of the structure of these inputs | |
| 114 # These inputs are populated in the Galaxy Wrapper xml and are in this format: | |
| 115 # ARRAY[DICT{FILE_PATH: DICT{NAME: NAME_VALUE, EXTRA_DATA: EXTRA_DATA_VALUE}}] | |
| 116 # EXTRA_DATA could be anything, for example the index of a BAM => {"index", FILE_PATH} | |
| 117 array_inputs_bam = args.bam | |
| 118 array_inputs_bed_generic = args.bed | |
| 119 array_inputs_bed_simple_repeats = args.bedSimpleRepeats | |
| 120 array_inputs_bed_splice_junctions = args.bedSpliceJunctions | |
| 121 array_inputs_bigwig = args.bigwig | |
| 122 array_inputs_gff3 = args.gff3 | |
| 123 array_inputs_gtf = args.gtf | |
| 124 array_inputs_psl = args.psl | |
| 125 | |
| 126 outputFile = args.output | |
| 127 | |
| 128 json_inputs_data = args.data_json | |
| 129 | |
| 130 # TODO: Instead use a class to properly store the objects, with object_hook | |
| 131 inputs_data = json.loads(json_inputs_data) | |
| 132 # We remove the spaces in ["name"] of inputs_data | |
| 133 sanitize_name_inputs(inputs_data) | |
| 134 | |
| 135 # TODO: Check here all the binaries / tools we need. Exception if missing | |
| 136 | |
| 137 # Create the Track Hub folder | |
| 138 trackHub = TrackHub(reference_genome, user_email, outputFile, extra_files_path, toolDirectory) | |
| 139 | |
| 140 all_datatype_dictionary = {} | |
| 141 | |
| 142 for (inputs, datatype_class) in [ | |
| 143 (array_inputs_bam, Bam), | |
| 144 (array_inputs_bed_generic, Bed), | |
| 145 (array_inputs_bigwig, BigWig), | |
| 146 (array_inputs_bed_simple_repeats, BedSimpleRepeats), | |
| 147 (array_inputs_bed_splice_junctions, BedSpliceJunctions), | |
| 148 (array_inputs_gff3, Gff3), | |
| 149 (array_inputs_gtf, Gtf), | |
| 150 (array_inputs_psl, Psl)]: | |
| 151 if inputs: | |
| 152 all_datatype_dictionary.update(create_ordered_datatype_objects(datatype_class, inputs, inputs_data)) | |
| 153 | |
| 154 # Create Ordered Dictionary to add the tracks in the tool form order | |
| 155 all_datatype_ordered_dictionary = collections.OrderedDict(all_datatype_dictionary) | |
| 156 | |
| 157 logging.debug("----- End of all_datatype_dictionary processing -----") | |
| 158 logging.debug("all_datatype_ordered_dictionary keys are: {0}".format(all_datatype_ordered_dictionary.values())) | |
| 159 | |
| 160 logging.debug("----- Beginning of Track adding processing -----") | |
| 161 for index, datatypeObject in all_datatype_ordered_dictionary.iteritems(): | |
| 162 trackHub.addTrack(datatypeObject.track.trackDb) | |
| 163 logging.debug("----- End of Track adding processing -----") | |
| 164 | |
| 165 # We process all the modifications to create the zip file | |
| 166 #trackHub.createZip() | |
| 167 | |
| 168 # We terminate le process and so create a HTML file summarizing all the files | |
| 169 trackHub.terminate() | |
| 170 | |
| 171 logging.debug('#### End of HubArchiveCreator Debug Mode: Bye! ####') | |
| 172 | |
| 173 sys.exit(0) | |
| 174 | |
| 175 | |
| 176 def sanitize_name_input(string_to_sanitize): | |
| 177 """ | |
| 178 Sanitize the string passed in parameter by replacing '/' and ' ' by '_' | |
| 179 | |
| 180 :param string_to_sanitize: | |
| 181 :return : | |
| 182 | |
| 183 :Example: | |
| 184 | |
| 185 >>> sanitize_name_input('this/is an//example') | |
| 186 this_is_an__example | |
| 187 """ | |
| 188 return string_to_sanitize \ | |
| 189 .replace("/", "_") \ | |
| 190 .replace(" ", "_") | |
| 191 | |
| 192 | |
| 193 def sanitize_name_inputs(inputs_data): | |
| 194 """ | |
| 195 Sanitize value of the keys "name" of the dictionary passed in parameter. | |
| 196 | |
| 197 Because sometimes output from Galaxy, or even just file name, from user inputs, have spaces. | |
| 198 Also, it can contain '/' character and could break the use of os.path function. | |
| 199 | |
| 200 :param inputs_data: dict[string, dict[string, string]] | |
| 201 """ | |
| 202 for key in inputs_data: | |
| 203 inputs_data[key]["name"] = sanitize_name_input(inputs_data[key]["name"]) | |
| 204 | |
| 205 | |
| 206 def create_ordered_datatype_objects(ExtensionClass, array_inputs, inputs_data): | |
| 207 """ | |
| 208 Function which executes the creation all the necessary files / folders for a special Datatype, for TrackHub | |
| 209 and update the dictionary of datatype | |
| 210 | |
| 211 :param ExtensionClass: | |
| 212 :param array_inputs: | |
| 213 :param inputs_data: | |
| 214 :type ExtensionClass: Datatype | |
| 215 :type array_inputs: list[string] | |
| 216 :type inputs_data: dict | |
| 217 :rtype: dict | |
| 218 """ | |
| 219 | |
| 220 datatype_dictionary = {} | |
| 221 | |
| 222 # TODO: Optimize this double loop | |
| 223 for input_false_path in array_inputs: | |
| 224 for key, data_value in inputs_data.items(): | |
| 225 if key == input_false_path: | |
| 226 logging.debug("input_false_path: " + input_false_path) | |
| 227 logging.debug("data_value: " + str(data_value)) | |
| 228 extensionObject = ExtensionClass(input_false_path, data_value) | |
| 229 datatype_dictionary.update({data_value["order_index"]: extensionObject}) | |
| 230 return datatype_dictionary | |
| 231 | |
| 232 def configure_logger(extra_files_path=None, debug=False): | |
| 233 if not extra_files_path: | |
| 234 raise Exception("Extra files path is not set. Stopping the application") | |
| 235 | |
| 236 | |
| 237 # All case log: log everything in a .log file | |
| 238 logger_file_name = ''.join([__name__, '.log']) | |
| 239 logging_file_path = os.path.join(extra_files_path, logger_file_name) | |
| 240 | |
| 241 logging.basicConfig(filename=logging_file_path, level=logging.DEBUG) | |
| 242 | |
| 243 log_stdout = logging.StreamHandler(sys.stdout) | |
| 244 if not debug: | |
| 245 configure_logger_user(log_stdout) | |
| 246 else: | |
| 247 configure_logger_dev(log_stdout) | |
| 248 | |
| 249 # stderr configuration | |
| 250 configure_logger_stderr() | |
| 251 | |
| 252 logging.debug('#### Welcome in HubArchiveCreator Debug Mode ####\n') | |
| 253 | |
| 254 def configure_logger_user(log_stdout=None): | |
| 255 """ | |
| 256 User Logger is defined as following: | |
| 257 - User needs to have WARN, ERROR and CRITICAL but well formatted / without traceback | |
| 258 in STDOUT | |
| 259 - Still access to full, brute and traceback for errors | |
| 260 in STDERR | |
| 261 - And further access to debug if needed | |
| 262 in .log | |
| 263 | |
| 264 """ | |
| 265 | |
| 266 if not log_stdout: | |
| 267 raise Exception("No log_stdout given. Stopping the application") | |
| 268 | |
| 269 # stdout for INFO / WARN / ERROR / CRITICAL | |
| 270 log_stdout.setLevel(logging.INFO) | |
| 271 | |
| 272 formatter = TraceBackFormatter('%(message)s') | |
| 273 | |
| 274 log_stdout.setFormatter(formatter) | |
| 275 | |
| 276 logging.getLogger().addHandler(log_stdout) | |
| 277 | |
| 278 def configure_logger_dev(log_stdout=None): | |
| 279 """ | |
| 280 Dev Logger is defined as following: | |
| 281 - Dev needs to have WARN, ERROR and CRITICAL but well formatted / without traceback, in stdout | |
| 282 - Still access to full, brute and traceback in stderr for errors | |
| 283 - And further access to debug if needed | |
| 284 | |
| 285 """ | |
| 286 if not log_stdout: | |
| 287 raise Exception("No log_stdout given. Stopping the application") | |
| 288 log_format = '%(message)s' | |
| 289 | |
| 290 # stdout and stderr and both identical for INFO / WARN / ERROR / CRITICAL | |
| 291 log_stdout.setLevel(logging.DEBUG) | |
| 292 | |
| 293 formatter = logging.Formatter(log_format) | |
| 294 | |
| 295 log_stdout.setFormatter(formatter) | |
| 296 | |
| 297 logging.getLogger().addHandler(log_stdout) | |
| 298 | |
| 299 def configure_logger_stderr(): | |
| 300 """ | |
| 301 Configure what should be logged in stderr | |
| 302 """ | |
| 303 log_error = logging.StreamHandler(sys.stderr) | |
| 304 log_error.setLevel(logging.ERROR) | |
| 305 log_error_format = '%(message)s' | |
| 306 | |
| 307 formatter_error = logging.Formatter(log_error_format) | |
| 308 | |
| 309 log_error.setFormatter(formatter_error) | |
| 310 | |
| 311 logging.getLogger().addHandler(log_error) | |
| 312 | |
| 313 if __name__ == "__main__": | |
| 314 logging.getLogger(__name__) | |
| 315 main(sys.argv) |
