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author | yating-l |
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date | Wed, 21 Dec 2016 12:13:04 -0500 |
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<tool id="hubArchiveCreator" name="Hub Archive Creator" version="2.3.0"> <description> This Galaxy tool permits to prepare your files to be ready for Assembly Hub visualization. </description> <requirements> <!-- Conda dependencies --> <requirement type="package" version="332">ucsc-bedtobigbed</requirement> <requirement type="package" version="332">ucsc-fatotwobit</requirement> <requirement type="package" version="332">ucsc-genepredtobed</requirement> <requirement type="package" version="332">ucsc-genepredtobiggenepred</requirement> <requirement type="package" version="332">ucsc-gff3togenepred</requirement> <requirement type="package" version="332">ucsc-gtftogenepred</requirement> <!-- TODO: Change the conda ucsc-psltobigpsl and take one > v337 because of bugs before --> <!-- <requirement type="package" version="332">ucsc-psltobigpsl</requirement> --> <requirement type="package" version="332">ucsc-twobitinfo</requirement> <requirement type="package" version="1.3">samtools</requirement> <!-- ToolShed dependencies --> <requirement type="package" version="312">ucsc_tools</requirement> <requirement type="package" version="0.0.1">gff3ToGenePred</requirement> <requirement type="package" version="0.0.1">gtfToGenePred</requirement> <requirement type="package" version="0.0.1">genePredToBed</requirement> <requirement type="package" version="0.0.1">genePredToBigGenePred</requirement> <requirement type="package" version="0.0.1">pslToBigPsl</requirement> <requirement type="package" version="1.2">samtools</requirement> </requirements> <stdio> <regex match="^pass1" source="stderr" level="log" description="bedToBigBed"/> <!-- TODO: Add the case pass1 and 0 chroms --> <!-- TODO: Add the case pass2 and 0 records or 0 fields --> </stdio> <!-- Idea: python \ -augustus [parameters] \ -trfBig [parameters] --> <command detect_errors="exit_code"><![CDATA[ mkdir -p $output.extra_files_path; python $__tool_directory__/hubArchiveCreator.py ## Ask the user to enter the genome name --genome_name '$genome_name' #import json #set global data_parameter_dict = {} ## Function to retrieve the data of the inputs #def prepare_json($input_to_prepare, $order_index, $extra_data_dict={}) #set false_path = str($input_to_prepare) #set name = $input_to_prepare.name #set data_dict = {"name": $name} #silent data_dict.update($extra_data_dict) ## Add the ordering by taking the tool form indexes #silent $data_dict.update({"order_index": $order_index}) #silent $data_parameter_dict.update({$false_path: $data_dict}) #end def ## Get the number of digits from tracks, to have a unique integer from group index and track index #set temp_max_digit = 0 #for $g in $group #if len($g.format) > $temp_max_digit #silent temp_max_digit = len($g.format) #end if #end for #set nb_digits_max_track = len(str($temp_max_digit)) ## END Get the number of digits #for $i_g, $g in enumerate( $group ) #for $i, $f in enumerate( $g.format ) ## Create the order index using index_group+1 concatenated with index_track #set index_group_final = str($i_g + 1) #set index_track_final = str($index_group_final) + str($i).zfill($nb_digits_max_track) ## For each format, we have a few mandatory fields we store in a dict #set track_color = str($f.formatChoice.track_color) #set group_name = str($g.group_name) #set extra_data_dict = {"track_color": $track_color, "group_name": $group_name} #if $f.formatChoice.format_select == "bam" --bam $f.formatChoice.BAM #set bam_index = $f.formatChoice.BAM.metadata.bam_index ## Add Bam format specific fields #silent extra_data_dict.update({"index": $bam_index}) #silent $prepare_json($f.formatChoice.BAM, $index_track_final, extra_data_dict) #end if #if $f.formatChoice.format_select == "bed" #if $f.formatChoice.bedChoice.bed_select == "bed_generic" --bed $f.formatChoice.bedChoice.BED #silent $prepare_json($f.formatChoice.bedChoice.BED, $index_track_final, extra_data_dict) #end if #if $f.formatChoice.bedChoice.bed_select == "bed_simple_repeats_option" --bedSimpleRepeats $f.formatChoice.bedChoice.BED_simple_repeats #silent $prepare_json($f.formatChoice.bedChoice.BED_simple_repeats, $index_track_final, extra_data_dict) #end if #if $f.formatChoice.bedChoice.bed_select == "bed_splice_junctions_option" --bedSpliceJunctions $f.formatChoice.bedChoice.Bed_splice_junctions #silent $prepare_json($f.formatChoice.bedChoice.BED_splice_junctions, $index_track_final, extra_data_dict) #end if #end if #if $f.formatChoice.format_select == "psl" --psl $f.formatChoice.PSL #silent $prepare_json($f.formatChoice.PSL, $index_track_final, extra_data_dict) #end if #if $f.formatChoice.format_select == "bigwig" --bigwig $f.formatChoice.BIGWIG #silent $prepare_json($f.formatChoice.BIGWIG, $index_track_final, extra_data_dict) #end if #if $f.formatChoice.format_select == "gff3" --gff3 $f.formatChoice.GFF3 #silent $prepare_json($f.formatChoice.GFF3, $index_track_final, extra_data_dict) #end if #if $f.formatChoice.format_select == "gtf" ## Add also GTF from Agustus? See https://github.com/ENCODE-DCC/kentUtils/issues/8 --gtf $f.formatChoice.GTF #silent $prepare_json($f.formatChoice.GTF, $index_track_final, extra_data_dict) #end if #end for #end for ## We combine the fasta file dataset name with his false path in a JSON object #set fasta_json = json.dumps({"false_path": str($fasta_file), "name": $fasta_file.name}) -f '$fasta_json' ## Dump the final json #set all_data_json = json.dumps($data_parameter_dict) --data_json '$all_data_json' ## Retrieve the user email --user_email $__user_email__ -d $__tool_directory__ -e $output.extra_files_path $advanced_options.debug_mode -o $output; ]]></command> <inputs> <param name="genome_name" type="text" size="30" value="unknown" label="UCSC Genome Browser assembly ID" /> <param format="fasta" name="fasta_file" type="data" label="Reference genome" /> <repeat name="group" title="New group"> <param type="text" name="group_name" label="Group name" value="Default group"/> <repeat name="format" title="New track"> <conditional name="formatChoice"> <param name="format_select" type="select" label="Format"> <option value="bam" selected="true">BAM</option> <option value="bed">BED</option> <option value="psl">PSL</option> <option value="bigwig">BIGWIG</option> <option value="gff3">GFF3</option> <option value="gtf">GTF</option> </param> <when value="bam"> <param format="bam" name="BAM" type="data" label="BAM File" /> <!-- TODO: Find a solution to avoid repetition and to generate a new color depending on the others --> <param name="track_color" type="color" label="Track color" value="#000000"> <sanitizer> <valid initial="string.letters,string.digits"> <add value="#"/> </valid> </sanitizer> </param> </when> <when value="bed"> <conditional name="bedChoice"> <param name="bed_select" type="select" label="Bed Choice"> <option value="bed_generic" selected="true">BED Generic (bed3+)</option> <option value="bed_simple_repeats_option">BED Simple repeat (bed4+12 / simpleRepeat.as)</option> <option value="bed_splice_junctions_option">BED Splice junctions (bed12+1 / spliceJunctions.as)</option> </param> <when value="bed_generic"> <param format="bed" name="BED" type="data" label="Generic Bed File Choice" /> </when> <when value="bed_simple_repeats_option"> <param format="bed" name="BED_simple_repeats" type="data" label="Bed Simple Repeats (Bed4+12) File" /> </when> <when value="bed_splice_junctions_option"> <param format="bed" name="BED_splice_junctions" type="data" label="Bed Splice Junctions (Bed12+1) File" /> </when> </conditional> <param name="track_color" type="color" label="Track color" value="#000000"> <sanitizer> <valid initial="string.letters,string.digits"> <add value="#"/> </valid> </sanitizer> </param> </when> <when value="psl"> <param format="psl" name="PSL" type="data" label="PSL File" /> <param name="track_color" type="color" label="Track color" value="#000000"> <sanitizer> <valid initial="string.letters,string.digits"> <add value="#"/> </valid> </sanitizer> </param> </when> <when value="bigwig"> <param format="bigwig" name="BIGWIG" type="data" label="BIGWIG File" /> <param name="track_color" type="color" label="Track color" value="#000000"> <sanitizer> <valid initial="string.letters,string.digits"> <add value="#"/> </valid> </sanitizer> </param> </when> <when value="gff3"> <param format="gff3" name="GFF3" type="data" label="GFF3 File" /> <param name="track_color" type="color" label="Track color" value="#000000"> <sanitizer> <valid initial="string.letters,string.digits"> <add value="#"/> </valid> </sanitizer> </param> </when> <when value="gtf"> <param format="gtf" name="GTF" type="data" label="GTF File" /> <param name="track_color" type="color" label="Track color" value="#000000"> <sanitizer> <valid initial="string.letters,string.digits"> <add value="#"/> </valid> </sanitizer> </param> </when> </conditional> </repeat> </repeat> <conditional name="advanced_options"> <param name="advanced_options_selector" type="select" label="Advanced options"> <option value="off" selected="true">Hide advanced options</option> <option value="on">Display advanced options</option> </param> <!-- TODO: Avoid redundancy here --> <when value="on"> <param name="debug_mode" type="boolean" label="Activate debug mode" checked="false" truevalue="--debug_mode" falsevalue=""> <help> Use this option if you are a G-OnRamp developer </help> </param> </when> <when value="off"> <param name="debug_mode" type="hidden" value=""> </param> </when> </conditional> </inputs> <outputs> <data format="trackhub" name="output"/> </outputs> <tests> <!-- Test with only the fasta file --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <output name="output" file="only_genome/only_genome.html"> <extra_files type="file" name="__main__.log" value="only_genome/__main__.log" /> <extra_files type="file" name="myHub/genomes.txt" value="only_genome/myHub/genomes.txt" /> <extra_files type="file" name="myHub/Dbia3.html" value="only_genome/myHub/Dbia3.html"/> <!-- Email could be different, but we need to ensure we still have the email line --> <extra_files type="file" name="myHub/hub.txt" value="only_genome/myHub/hub.txt" lines_diff="2"> <assert_contents> <has_text text="email"/> </assert_contents> </extra_files> <extra_files type="file" name="myHub/Dbia3/Dbia3.2bit" value="common/Dbia3.2bit"> </extra_files> <extra_files type="file" name="myHub/Dbia3/description.html" value="only_genome/myHub/Dbia3/description.html"/> </output> </test> <!-- Test with Bam --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="bam"/> <param name="BAM" value="bam/inputs/HISAT2_Accepted_Hits.bam" /> <param name="track_color" value="#000000"/> </conditional> </repeat> </repeat> <output name="output" file="bam/bam.html"> <!-- Verify tracks folder contains bam and bai --> <extra_files type="file" name="myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam" value="bam/myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam" compare="sim_size" /> <extra_files type="file" name="myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam.bai" value="bam/myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam.bai" compare="sim_size" /> <!-- Verify trackDb.txt contains the basic fields for a bam --> <extra_files type="file" name="myHub/Dbia3/trackDb.txt" value="bam/myHub/Dbia3/trackDb.txt"> <assert_contents> <has_text text="track"/> <has_text text="longLabel"/> <has_text text="shortLabel"/> <has_text text="bigDataUrl"/> <has_text text="type"/> <has_text text="visibility"/> <has_text text="thickDrawItem"/> <has_text text="priority"/> <has_text text="color"/> <has_text text="group"/> </assert_contents> </extra_files> <!-- TODO: Find a way to check also that the whole common structure is intact too, without too much repetition --> </output> </test> <!-- Test with Bed Generic --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="bed" /> <conditional name="bedChoice"> <param name="bed_select" value="bed_generic"/> <param name="BED" ftype="bed" value="bed_generic/inputs/TBLASTN_Alignment_to_proteins"/> <param name="track_color" value="#000000"/> </conditional> </conditional> </repeat> </repeat> <output name="output" file="bed_generic/bed_generic.html"> <!-- Verify tracks folder contains bam and bai --> <extra_files type="file" name="myHub/Dbia3/tracks/TBLASTN_Alignment_to_proteins.bb" value="bed_generic/myHub/Dbia3/tracks/TBLASTN_Alignment_to_proteins.bb" compare="sim_size" /> </output> </test> <!-- Test with Bed Simple repeat --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="bed" /> <conditional name="bedChoice"> <param name="bed_select" value="bed_simple_repeats_option"/> <param name="BED_simple_repeats" ftype="bed" value="bed_simple_repeats/inputs/Repeating_Elements_by_TrfBig"/> <param name="track_color" value="#000000"/> </conditional> </conditional> </repeat> </repeat> <output name="output" file="bed_simple_repeats/bed_simple_repeats_trackhub.html"> <!-- Verify tracks folder contains bam and bai --> <extra_files type="file" name="myHub/Dbia3/tracks/Repeating_Elements_by_TrfBig.bb" value="bed_simple_repeats/myHub/Dbia3/tracks/Repeating_Elements_by_TrfBig.bb" compare="sim_size" /> </output> </test> <!-- Test with Psl --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="psl"/> <param name="PSL" value="psl/inputs/blastXmlToPsl"/> <param name="track_color" value="#000000"/> </conditional> </repeat> </repeat> <output name="output" file="psl/psl_trackhub.html"> <!-- Verify tracks folder contains bam and bai --> <extra_files type="file" name="myHub/Dbia3/tracks/blastXmlToPsl.bb" value="psl/myHub/Dbia3/tracks/blastXmlToPsl.bb" compare="sim_size" /> <!-- Verify trackDb.txt contains the basic fields for a bam --> <!-- TODO: Find a way to check also that the whole common structure is intact too, without too much repetition --> </output> </test> <!-- Test with BigWig --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="bigwig"/> <param name="BIGWIG" value="bigwig/inputs/RNA-Seq_Alignment_Summary"/> <param name="track_color" value="#000000"/> </conditional> </repeat> </repeat> <output name="output" file="bigwig/bigwig.html"> <!-- Verify tracks folder contains bam and bai --> <extra_files type="file" name="myHub/Dbia3/tracks/RNA-Seq_Alignment_Summary.bigwig" value="bigwig/myHub/Dbia3/tracks/RNA-Seq_Alignment_Summary.bigwig" compare="sim_size" /> <!-- Verify trackDb.txt contains the basic fields for a bam --> <!-- TODO: Find a way to check also that the whole common structure is intact too, without too much repetition --> </output> </test> <!-- Test with GFF3 --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="gff3"/> <param name="GFF3" value="gff3/inputs/Augustus_Gene_Predictions"/> <param name="track_color" value="#000000"/> </conditional> </repeat> </repeat> <output name="output" file="gff3/gff3_trackhub.html"> <!-- Verify tracks folder contains bam and bai --> <extra_files type="file" name="myHub/Dbia3/tracks/Augustus_Gene_Predictions.bb" value="gff3/myHub/Dbia3/tracks/Augustus_Gene_Predictions.bb" compare="sim_size" /> <!-- Verify trackDb.txt contains the basic fields for a bam --> <!-- TODO: Find a way to check also that the whole common structure is intact too, without too much repetition --> </output> </test> <!-- Test with GTF --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="gtf"/> <param name="GTF" value="gtf/inputs/StringTie_Assembled_Transcripts"/> <param name="track_color" value="#000000"/> </conditional> </repeat> </repeat> <output name="output" file="gtf/gtf_trackhub.html"> <!-- Verify tracks folder contains bam and bai --> <extra_files type="file" name="myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb" value="gtf/myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb" compare="sim_size" /> <!-- Verify trackDb.txt contains the basic fields for a bam --> <!-- TODO: Find a way to check also that the whole common structure is intact too, without too much repetition --> </output> </test> <!-- Test with one group and multiple tracks --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="gtf"/> <param name="GTF" value="gtf/inputs/StringTie_Assembled_Transcripts"/> <param name="track_color" value="#000000"/> </conditional> </repeat> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="gff3"/> <param name="GFF3" value="gff3/inputs/Augustus_Gene_Predictions"/> <param name="track_color" value="#000000"/> </conditional> </repeat> </repeat> <output name="output" file="gtf_gff/gtf_gff_trackhub.html"> <!-- verify tracks folder contains bam and bai --> <extra_files type="file" name="myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb" value="gtf/myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb" compare="sim_size" /> <extra_files type="file" name="myHub/Dbia3/tracks/Augustus_Gene_Predictions.bb" value="gff3/myHub/Dbia3/tracks/Augustus_Gene_Predictions.bb" compare="sim_size" /> <!-- verify trackdb.txt contains the basic fields for a bam --> <!-- todo: find a way to check also that the whole common structure is intact too, without too much repetition --> </output> </test> <!-- Test with one group and all the supported datatypes on 10/04/2016 --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="bam"/> <param name="BAM" value="bam/inputs/HISAT2_Accepted_Hits.bam"/> <param name="track_color" value="#000000"/> </conditional> </repeat> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="bed"/> <conditional name="bedChoice"> <param name="bed_select" value="bed_generic"/> <param name="BED" ftype="bed" value="bed_generic/inputs/TBLASTN_Alignment_to_proteins"/> <param name="track_color" value="#000000"/> </conditional> </conditional> </repeat> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="bed"/> <conditional name="bedChoice"> <param name="bed_select" value="bed_simple_repeats_option"/> <param name="BED_simple_repeats" ftype="bed" value="bed_simple_repeats/inputs/Repeating_Elements_by_TrfBig"/> <param name="track_color" value="#000000"/> </conditional> </conditional> </repeat> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="psl"/> <param name="PSL" value="psl/inputs/blastXmlToPsl"/> <param name="track_color" value="#000000"/> </conditional> </repeat> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="bigwig"/> <param name="BIGWIG" value="bigwig/inputs/RNA-Seq_Alignment_Summary"/> <param name="track_color" value="#000000"/> </conditional> </repeat> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="gff3"/> <param name="GFF3" value="gff3/inputs/Augustus_Gene_Predictions"/> <param name="track_color" value="#000000"/> </conditional> </repeat> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="gtf"/> <param name="GTF" value="gtf/inputs/StringTie_Assembled_Transcripts"/> <param name="track_color" value="#000000"/> </conditional> </repeat> </repeat> <output name="output" file="all_datatypes/all_datatypes_trackhub.html"> <!-- verify tracks folder contains all the files --> <extra_files type="file" name="myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam" value="all_datatypes/myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam" compare="sim_size" /> <extra_files type="file" name="myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam.bai" value="all_datatypes/myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam.bai" compare="sim_size" /> <extra_files type="file" name="myHub/Dbia3/tracks/TBLASTN_Alignment_to_proteins.bb" value="all_datatypes/myHub/Dbia3/tracks/TBLASTN_Alignment_to_proteins.bb" compare="sim_size" /> <extra_files type="file" name="myHub/Dbia3/tracks/Repeating_Elements_by_TrfBig.bb" value="all_datatypes/myHub/Dbia3/tracks/Repeating_Elements_by_TrfBig.bb" compare="sim_size" /> <extra_files type="file" name="myHub/Dbia3/tracks/blastXmlToPsl.bb" value="all_datatypes/myHub/Dbia3/tracks/blastXmlToPsl.bb" compare="sim_size" /> <extra_files type="file" name="myHub/Dbia3/tracks/RNA-Seq_Alignment_Summary.bigwig" value="all_datatypes/myHub/Dbia3/tracks/RNA-Seq_Alignment_Summary.bigwig" compare="sim_size" /> <extra_files type="file" name="myHub/Dbia3/tracks/Augustus_Gene_Predictions.bb" value="all_datatypes/myHub/Dbia3/tracks/Augustus_Gene_Predictions.bb" compare="sim_size" /> <extra_files type="file" name="myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb" value="all_datatypes/myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb" compare="sim_size" /> <!-- verify trackdb.txt contains the basic fields for a bam --> <!-- todo: find a way to check also that the whole common structure is intact too, without too much repetition --> </output> </test> <!-- Test with two groups and no tracks --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> </repeat> <repeat name="group"> <param name="group_name" value="Other group"/> </repeat> <output name="output" file="two_groups_no_track/two_groups_no_track_trackhub.html"> <extra_files type="file" name="myHub/genomes.txt" value="two_groups_no_track/myHub/genomes.txt"/> <extra_files type="file" name="myHub/Dbia3.html" value="two_groups_no_track/myHub/Dbia3.html"/> <!-- Email could be different, but we need to ensure we still have the email line --> <extra_files type="file" name="myHub/hub.txt" value="two_groups_no_track/myHub/hub.txt" lines_diff="2"> <assert_contents> <has_text text="email"/> </assert_contents> </extra_files> <extra_files type="file" name="myHub/Dbia3/Dbia3.2bit" value="common/Dbia3.2bit"> </extra_files> <extra_files type="file" name="myHub/Dbia3/description.html" value="two_groups_no_track/myHub/Dbia3/description.html"/> </output> </test> <!-- Test with two groups and one track in first --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="bigwig"/> <param name="BIGWIG" value="bigwig/inputs/RNA-Seq_Alignment_Summary"/> <param name="track_color" value="#000000"/> </conditional> </repeat> </repeat> <repeat name="group"> <param name="group_name" value="Other group"/> </repeat> <output name="output" file="two_groups_one_track_first/two_groups_one_track_first_trackhub.html"> <!-- Check myHub structure --> <extra_files type="file" name="myHub/genomes.txt" value="two_groups_one_track_first/myHub/genomes.txt"/> <extra_files type="file" name="myHub/Dbia3.html" value="two_groups_one_track_first/myHub/Dbia3.html"/> <!-- Email could be different, but we need to ensure we still have the email line --> <extra_files type="file" name="myHub/hub.txt" value="two_groups_one_track_first/myHub/hub.txt" lines_diff="2"> <assert_contents> <has_text text="email"/> </assert_contents> </extra_files> <extra_files type="file" name="myHub/Dbia3/Dbia3.2bit" value="common/Dbia3.2bit"> </extra_files> <extra_files type="file" name="myHub/Dbia3/description.html" value="two_groups_one_track_first/myHub/Dbia3/description.html"/> <!-- Check tracks exist --> <extra_files type="file" name="myHub/Dbia3/tracks/RNA-Seq_Alignment_Summary.bigwig" value="bigwig/myHub/Dbia3/tracks/RNA-Seq_Alignment_Summary.bigwig" compare="sim_size" /> <!-- Check the groups.txt exists and is properly populated --> </output> </test> <!-- Test with two groups and one track in both --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="bigwig"/> <param name="BIGWIG" value="bigwig/inputs/RNA-Seq_Alignment_Summary"/> <param name="track_color" value="#000000"/> </conditional> </repeat> </repeat> <repeat name="group"> <param name="group_name" value="Other group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="gtf"/> <param name="GTF" value="gtf/inputs/StringTie_Assembled_Transcripts"/> <param name="track_color" value="#000000"/> </conditional> </repeat> </repeat> <output name="output" file="two_groups_one_track_both/two_groups_one_track_both_trackhub.html"> <!-- Check myHub structure --> <extra_files type="file" name="myHub/genomes.txt" value="two_groups_one_track_both/myHub/genomes.txt"/> <extra_files type="file" name="myHub/Dbia3.html" value="two_groups_one_track_both/myHub/Dbia3.html"/> <!-- Email could be different, but we need to ensure we still have the email line --> <extra_files type="file" name="myHub/hub.txt" value="two_groups_one_track_both/myHub/hub.txt" lines_diff="2"> <assert_contents> <has_text text="email"/> </assert_contents> </extra_files> <extra_files type="file" name="myHub/Dbia3/Dbia3.2bit" value="common/Dbia3.2bit"> </extra_files> <extra_files type="file" name="myHub/Dbia3/description.html" value="two_groups_one_track_both/myHub/Dbia3/description.html"/> <!-- Check tracks exist --> <!-- First group --> <extra_files type="file" name="myHub/Dbia3/tracks/RNA-Seq_Alignment_Summary.bigwig" value="bigwig/myHub/Dbia3/tracks/RNA-Seq_Alignment_Summary.bigwig" compare="sim_size" /> <!-- Second group --> <extra_files type="file" name="myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb" value="gtf/myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb" compare="sim_size" /> <!-- Check the groups.txt exists and is properly populated --> </output> </test> <!-- Test with two groups and multiple tracks in both --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="bigwig"/> <param name="BIGWIG" value="bigwig/inputs/RNA-Seq_Alignment_Summary"/> <param name="track_color" value="#000000"/> </conditional> </repeat> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="bam"/> <param name="BAM" value="bam/inputs/HISAT2_Accepted_Hits.bam"/> <param name="track_color" value="#000000"/> </conditional> </repeat> </repeat> <repeat name="group"> <param name="group_name" value="Other group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="gtf"/> <param name="GTF" value="gtf/inputs/StringTie_Assembled_Transcripts"/> <param name="track_color" value="#000000"/> </conditional> </repeat> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="gff3"/> <param name="GFF3" value="gff3_multi_fasta/inputs/Multi-Fasta_GlimmerHMM_Gene_Predictions"/> <param name="track_color" value="#000000"/> </conditional> </repeat> </repeat> <output name="output" file="two_groups_multiple_tracks/two_groups_multiple_tracks_trackhub.html"> <!-- Check myHub structure --> <extra_files type="file" name="myHub/genomes.txt" value="two_groups_multiple_tracks/myHub/genomes.txt"/> <extra_files type="file" name="myHub/Dbia3.html" value="two_groups_multiple_tracks/myHub/Dbia3.html"/> <!-- Email could be different, but we need to ensure we still have the email line --> <extra_files type="file" name="myHub/hub.txt" value="two_groups_multiple_tracks/myHub/hub.txt" lines_diff="2"> <assert_contents> <has_text text="email"/> </assert_contents> </extra_files> <extra_files type="file" name="myHub/Dbia3/Dbia3.2bit" value="common/Dbia3.2bit"> </extra_files> <extra_files type="file" name="myHub/Dbia3/description.html" value="two_groups_multiple_tracks/myHub/Dbia3/description.html"/> <!-- Check tracks exist --> <!-- First group --> <extra_files type="file" name="myHub/Dbia3/tracks/RNA-Seq_Alignment_Summary.bigwig" value="bigwig/myHub/Dbia3/tracks/RNA-Seq_Alignment_Summary.bigwig" compare="sim_size" /> <extra_files type="file" name="myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam" value="bam/myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam" compare="sim_size" /> <extra_files type="file" name="myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam.bai" value="bam/myHub/Dbia3/tracks/HISAT2_Accepted_Hits.bam.bai" compare="sim_size" /> <!-- Second group --> <extra_files type="file" name="myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb" value="gtf/myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb" compare="sim_size" /> <extra_files type="file" name="myHub/Dbia3/tracks/Multi-Fasta_GlimmerHMM_Gene_Predictions.bb" value="gff3_multi_fasta/myHub/Dbia3/tracks/Multi-Fasta_GlimmerHMM_Gene_Predictions.bb" compare="sim_size" /> <!-- Check the groups.txt exists and is properly populated --> </output> </test> <!-- Test default color in a track --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="bam"/> <param name="BAM" value="bam/inputs/HISAT2_Accepted_Hits.bam"/> <param name="track_color" value="#000000"/> </conditional> </repeat> </repeat> <output name="output" file="default_color/default_color_trackhub.html"> <!-- Verify trackDb.txt contains the color 0,0,0 --> <extra_files type="file" name="myHub/Dbia3/trackDb.txt" value="default_color/myHub/Dbia3/trackDb.txt"> <assert_contents> <has_text text="color 0,0,0"/> </assert_contents> </extra_files> <!-- TODO: Find a way to check also that the whole common structure is intact too, without too much repetition --> </output> </test> <!-- Test changed color in a track --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="bam"/> <param name="BAM" value="bam/inputs/HISAT2_Accepted_Hits.bam"/> <param name="track_color" value="#8064a2"/> </conditional> </repeat> </repeat> <output name="output" file="changed_color/changed_color_trackhub.html"> <!-- Verify trackDb.txt contains the color 128,100,162 --> <extra_files type="file" name="myHub/Dbia3/trackDb.txt" value="changed_color/myHub/Dbia3/trackDb.txt"> <assert_contents> <has_text text="color 128,100,162"/> </assert_contents> </extra_files> <!-- TODO: Find a way to check also that the whole common structure is intact too, without too much repetition --> </output> </test> <!-- Test for StringTie Chromosome end coordinates --> <test> <param name="genome_name" value="Dbia3"/> <param name="fasta_file" value="common/dbia3.fa"/> <repeat name="group"> <param name="group_name" value="Default group"/> <repeat name="format"> <conditional name="formatChoice"> <param name="format_select" value="gtf"/> <param name="GTF" ftype="gtf" value="stringtie_chromosome_end_coordinates/inputs/StringTie_Assembled_Transcripts"/> <param name="track_color" value="#000000"/> </conditional> </repeat> </repeat> <output name="output" file="stringtie_chromosome_end_coordinates/stringtie_chromosome_end_coordinates_trackhub.html"> <extra_files type="file" name="myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb" value="stringtie_chromosome_end_coordinates/myHub/Dbia3/tracks/StringTie_Assembled_Transcripts.bb" compare="sim_size" /> <!-- TODO: Find a way to check also that the whole common structure is intact too, without too much repetition --> </output> </test> <!-- Test for big files? --> <!-- Find tests that should fail --> </tests> <help> This Galaxy tool permits to prepare your files to be ready for Assembly Hub visualization. </help> </tool>