Mercurial > repos > yating-l > jbrowsearchivecreator
annotate TrackHub.py @ 9:7cb67cbbd8e5 draft
planemo upload for repository https://github.com/goeckslab/jbrowse-archive-creator.git commit 753ca09582fe0148e538103f2c211f7caa182966
author | yating-l |
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date | Tue, 01 May 2018 23:01:06 -0400 |
parents | 5d5fdcb798da |
children | 43a700afd457 |
rev | line source |
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0
804a93e87cc8
planemo upload for repository https://github.com/Yating-L/jbrowse_hub commit f22711ea7a464bdaf4d5aaea07f2eacf967aa66e-dirty
yating-l
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1 #!/usr/bin/env python |
804a93e87cc8
planemo upload for repository https://github.com/Yating-L/jbrowse_hub commit f22711ea7a464bdaf4d5aaea07f2eacf967aa66e-dirty
yating-l
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2 |
804a93e87cc8
planemo upload for repository https://github.com/Yating-L/jbrowse_hub commit f22711ea7a464bdaf4d5aaea07f2eacf967aa66e-dirty
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3 import os |
804a93e87cc8
planemo upload for repository https://github.com/Yating-L/jbrowse_hub commit f22711ea7a464bdaf4d5aaea07f2eacf967aa66e-dirty
yating-l
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4 import subprocess |
804a93e87cc8
planemo upload for repository https://github.com/Yating-L/jbrowse_hub commit f22711ea7a464bdaf4d5aaea07f2eacf967aa66e-dirty
yating-l
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5 import shutil |
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eda851e52060
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 479fc6490e24ca0e5034ae6c3579882e97e095e6-dirty
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6 import zipfile |
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804a93e87cc8
planemo upload for repository https://github.com/Yating-L/jbrowse_hub commit f22711ea7a464bdaf4d5aaea07f2eacf967aa66e-dirty
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7 import json |
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237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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8 import tempfile |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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9 import logging |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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10 from mako.lookup import TemplateLookup |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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11 |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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12 from datatypes.Datatype import Datatype |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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13 from tracks.TrackStyles import TrackStyles |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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14 from util import subtools |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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15 from util import santitizer |
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804a93e87cc8
planemo upload for repository https://github.com/Yating-L/jbrowse_hub commit f22711ea7a464bdaf4d5aaea07f2eacf967aa66e-dirty
yating-l
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16 |
804a93e87cc8
planemo upload for repository https://github.com/Yating-L/jbrowse_hub commit f22711ea7a464bdaf4d5aaea07f2eacf967aa66e-dirty
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17 |
804a93e87cc8
planemo upload for repository https://github.com/Yating-L/jbrowse_hub commit f22711ea7a464bdaf4d5aaea07f2eacf967aa66e-dirty
yating-l
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18 class TrackHub: |
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237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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19 def __init__(self, inputFastaFile, outputFile, extra_files_path, tool_directory, trackType): |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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20 |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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21 self.mySpecieFolderPath = None |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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22 |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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23 # Store intermediate files, will be removed if not in debug mode |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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24 self.myTracksFolderPath = None |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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25 |
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5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
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26 # Store interval files and their tabix index |
5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
yating-l
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27 self.myFinalTracksFolderPath = None |
5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
yating-l
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28 |
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237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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29 # Store binary files: Bam, BigWig |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
parents:
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30 self.myBinaryFolderPath = None |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
parents:
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31 |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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32 self.tool_directory = tool_directory |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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33 self.trackType = trackType |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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34 self.reference_genome = inputFastaFile |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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35 self.genome_name = inputFastaFile.assembly_id |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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36 self.extra_files_path = extra_files_path |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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37 self.outputFile = outputFile |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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38 self.chromSizesFile = None |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
parents:
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39 |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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parents:
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40 |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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parents:
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41 # Set all the missing variables of this class, and create physically the folders/files |
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5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
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42 self.__createAssemblyHub__(extra_files_path=extra_files_path) |
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237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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43 # Init the Datatype |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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44 Datatype.pre_init(self.reference_genome, self.chromSizesFile, |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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45 self.extra_files_path, self.tool_directory, |
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5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
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46 self.mySpecieFolderPath, self.myTracksFolderPath, self.myBinaryFolderPath, self.trackType) |
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237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
parents:
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47 |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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48 self._prepareRefseq() |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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49 self.trackList = os.path.join(self.mySpecieFolderPath, "trackList.json") |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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50 self._createTrackList() |
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5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
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51 if Datatype.trackType == 'HTMLFeatures': |
5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
yating-l
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52 self.myTrackStyle = TrackStyles(self.tool_directory, self.mySpecieFolderPath, self.trackList) |
6
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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53 self.logger = logging.getLogger(__name__) |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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54 |
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804a93e87cc8
planemo upload for repository https://github.com/Yating-L/jbrowse_hub commit f22711ea7a464bdaf4d5aaea07f2eacf967aa66e-dirty
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55 |
6
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planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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56 |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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57 def addTrack(self, trackDbObject): |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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58 if trackDbObject['dataType'].lower() == 'bam': |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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59 subtools.add_track_json(self.trackList, trackDbObject['options']) |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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60 elif trackDbObject['dataType'].lower() == 'bigwig': |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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61 subtools.add_track_json(self.trackList, trackDbObject['options']) |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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62 else: |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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63 if trackDbObject['trackType'] == 'HTMLFeatures': |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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64 self._customizeHTMLFeature(trackDbObject) |
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5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
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65 subtools.flatfile_to_json(trackDbObject['trackDataURL'], trackDbObject['dataType'], trackDbObject['trackType'], trackDbObject['trackLabel'], self.mySpecieFolderPath, trackDbObject['options']) |
5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
yating-l
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66 # Use Tabix index tracks by default for CanvasFeatures |
5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
yating-l
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67 # TODO: add support for HTMLFeatures |
5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
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68 else: |
5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
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69 subtools.generate_tabix_indexed_track(trackDbObject['trackDataURL'], trackDbObject['dataType'], trackDbObject['track'], self.myFinalTracksFolderPath) |
5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
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70 subtools.add_track_json(self.trackList, trackDbObject['options']) |
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237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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71 |
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5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
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72 def terminate(self, debug="false"): |
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planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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73 """ Write html file """ |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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74 self._indexName() |
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5d5fdcb798da
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit 12fb52d5b285935b2353d93a5aa291838df7893e
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75 if debug.lower() == "false": |
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planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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76 self._removeRaw() |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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77 self._outHtml() |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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78 print "Success!\n" |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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79 |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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80 |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
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81 def _customizeHTMLFeature(self, trackDbObject): |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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82 if trackDbObject['options']: |
237707a6b74d
planemo upload for repository https://github.com/Yating-L/jbrowse-archive-creator.git commit a500f7ab2119cc5faaf80393bd87428389d06880-dirty
yating-l
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83 subfeatures = trackDbObject['options'].get('subfeatureClasses') |
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84 feature_color = trackDbObject['options']['feature_color'] |
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85 if subfeatures: |
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86 for key, value in subfeatures.items(): |
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87 self.myTrackStyle.addCustomColor(value, feature_color) |
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88 else: |
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89 customizedFeature = santitizer.sanitize_name(trackDbObject['trackLabel']) |
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90 clientConfig = json.loads(trackDbObject['options']['clientConfig']) |
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91 clientConfig['renderClassName'] = customizedFeature |
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92 trackDbObject['options']['clientConfig'] = json.dumps(clientConfig) |
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93 self.myTrackStyle.addCustomColor(customizedFeature, feature_color) |
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94 |
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95 def _removeRaw(self): |
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96 if os.path.exists(self.myTracksFolderPath): |
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97 shutil.rmtree(self.myTracksFolderPath) |
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98 |
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99 def _createTrackList(self): |
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100 if not os.path.exists(self.trackList): |
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101 os.mknod(self.trackList) |
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102 |
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103 def _prepareRefseq(self): |
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104 subtools.prepare_refseqs(self.reference_genome.false_path, self.mySpecieFolderPath) |
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105 |
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106 def _indexName(self): |
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107 subtools.generate_names(self.mySpecieFolderPath) |
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108 print "finished name index \n" |
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109 |
6
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110 def _outHtml(self): |
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111 mylookup = TemplateLookup(directories=[os.path.join(self.tool_directory, 'templates')], |
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112 output_encoding='utf-8', encoding_errors='replace') |
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113 htmlTemplate = mylookup.get_template("display.txt") |
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114 |
6
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115 with open(self.outputFile, 'w') as htmlfile: |
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116 htmlMakoRendered = htmlTemplate.render( |
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117 jbrowse_hub_name = self.reference_genome.assembly_id, |
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118 trackList = os.path.relpath(self.trackList, self.extra_files_path) |
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119 ) |
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120 htmlfile.write(htmlMakoRendered) |
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121 |
6
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122 def __createAssemblyHub__(self, extra_files_path): |
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123 # Get all necessaries infos first |
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124 # 2bit file creation from input fasta |
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125 twoBitFile = tempfile.NamedTemporaryFile(bufsize=0) |
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126 subtools.faToTwoBit(self.reference_genome.false_path, twoBitFile.name) |
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127 |
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128 # Generate the twoBitInfo |
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129 twoBitInfoFile = tempfile.NamedTemporaryFile(bufsize=0) |
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130 subtools.twoBitInfo(twoBitFile.name, twoBitInfoFile.name) |
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131 |
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132 # Then we get the output to generate the chromSizes |
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133 self.chromSizesFile = tempfile.NamedTemporaryFile(bufsize=0, suffix=".chrom.sizes") |
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134 subtools.sortChromSizes(twoBitInfoFile.name, self.chromSizesFile.name) |
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135 |
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136 # We can get the biggest scaffold here, with chromSizesFile |
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137 with open(self.chromSizesFile.name, 'r') as chrom_sizes: |
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138 # TODO: Check if exists |
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139 self.default_pos = chrom_sizes.readline().split()[0] |
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140 |
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141 # Create the specie folder, use generic name "myHub", to support display JBrowse with Galaxy display application |
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142 mySpecieFolderPath = os.path.join(extra_files_path, "myHub") |
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143 if not os.path.exists(mySpecieFolderPath): |
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144 os.makedirs(mySpecieFolderPath) |
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145 self.mySpecieFolderPath = mySpecieFolderPath |
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146 |
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147 # Create the folder named 'raw' inside the specie folder to place raw files |
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148 tracksFolderPath = os.path.join(mySpecieFolderPath, "raw") |
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149 if not os.path.exists(tracksFolderPath): |
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150 os.makedirs(tracksFolderPath) |
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151 self.myTracksFolderPath = tracksFolderPath |
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152 |
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153 # Create the folder tracks into the specie folder |
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154 finalTracksFolderPath = os.path.join(mySpecieFolderPath, "tracks") |
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155 if not os.path.exists(finalTracksFolderPath): |
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156 os.makedirs(finalTracksFolderPath) |
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157 self.myFinalTracksFolderPath = finalTracksFolderPath |
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158 |
6
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159 myBinaryFolderPath = os.path.join(mySpecieFolderPath, 'bbi') |
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160 if not os.path.exists(myBinaryFolderPath): |
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161 os.makedirs(myBinaryFolderPath) |
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162 self.myBinaryFolderPath = myBinaryFolderPath |