Mercurial > repos > yating-l > twobit_mask_340
annotate twobit_mask.xml @ 0:fb318f9a36f3 draft default tip
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author | yating-l |
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date | Thu, 01 Jun 2017 14:10:35 -0400 |
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1 <?xml version="1.0"?> |
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2 <tool id="twobit_mask" name="twoBitMask" version="1.0"> |
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3 <description>Apply repeat masking to a twoBit file</description> |
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4 |
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5 <macros> |
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6 <import>ucsc_macros.xml</import> |
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7 </macros> |
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8 |
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9 <expand macro="requirements_twobit" /> |
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10 |
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11 <command detect_errors="exit_code"> |
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12 <![CDATA[ |
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13 #if str($maskfile_type.maskfile_type_selector) == "out": |
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14 ## twoBitMask expects the first column of the RepeatMasker header to |
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15 ## contain only three leading spaces. RepeatMasker output with large |
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16 ## SW scores could contain more leading spaces. |
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17 |
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18 awk '{ sub(/^\s+SW/, " SW"); print }' "${out_maskfile}" | |
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19 twoBitMask |
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20 ${add_mask} |
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21 -type=.out |
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22 "${twobit_input}" stdin "${twobit_output}" |
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23 #else: |
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24 ## The BED Output from TRF is in bed4+ format, but twoBitMask |
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25 ## only uses the first three fields of a BED file. |
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26 ## The extra columns result in a warning from twoBitMask |
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27 |
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28 awk 'BEGIN { OFS="\t" } { print $1, $2, $3 }' "${bed_maskfile}" | |
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29 twoBitMask |
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30 ${add_mask} |
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31 -type=.bed |
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32 "${twobit_input}" stdin "${twobit_output}" |
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33 #end if |
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34 ]]> |
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35 </command> |
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36 <inputs> |
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37 <param name="twobit_input" type="data" format="twobit" label="twoBit input file" /> |
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38 |
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39 <param name="add_mask" type="boolean" checked="true" |
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40 truevalue="-add" falsevalue="" |
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41 label="Keep pre-existing masking" |
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42 help="-add" /> |
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43 |
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44 <conditional name="maskfile_type"> |
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45 <param name="maskfile_type_selector" type="select" |
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46 label="Choose the type of mask file" |
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47 help="Mask file can be a RepeatMasker .out file or a BED file"> |
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48 <option value="out">RepeatMasker .out file</option> |
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49 <option value="bed" selected="true">BED file</option> |
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50 </param> |
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51 |
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52 <when value="out"> |
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53 <param name="out_maskfile" type="data" format="txt" |
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54 label="Mask file in RepeatMasker .out format" /> |
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55 </when> |
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56 |
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57 <when value="bed"> |
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58 <param name="bed_maskfile" type="data" format="bed" |
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59 label="Mask file in BED format" /> |
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60 </when> |
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61 </conditional> |
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62 </inputs> |
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63 <outputs> |
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64 <data name="twobit_output" format="twobit" /> |
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65 </outputs> |
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66 <tests> |
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67 <test> |
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68 <!-- Test standard RepeatMasker .out file --> |
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69 <param name="twobit_input" value="contig1.2bit" ftype="twobit" /> |
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70 <param name="maskfile_type_selector" value="out" /> |
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71 <param name="out_maskfile" value="contig1.fasta.out" /> |
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72 <output name="twobit_output" file="contig1.out.2bit" /> |
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73 </test> |
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74 <test> |
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75 <!-- Test RepeatMasker .out file with extra space in header --> |
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76 <param name="twobit_input" value="contig1.2bit" ftype="twobit" /> |
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77 <param name="maskfile_type_selector" value="out" /> |
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78 <param name="out_maskfile" value="contig1.fasta.extraspace.out" /> |
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79 <output name="twobit_output" file="contig1.out.2bit" /> |
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80 </test> |
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81 <test> |
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82 <!-- Test TRF .bed output --> |
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83 <param name="twobit_input" value="contig1.2bit" ftype="twobit" /> |
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84 <param name="maskfile_type_selector" value="bed" /> |
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85 <param name="bed_maskfile" value="contig1.fasta.bed" ftype="bed" /> |
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86 <output name="twobit_output" file="contig1.bed.2bit" /> |
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87 </test> |
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88 <test> |
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89 <!-- Test keep pre-existing masking --> |
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90 <param name="twobit_input" value="contig1.bed.2bit" ftype="twobit" /> |
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91 <param name="maskfile_type_selector" value="out" /> |
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92 <param name="out_maskfile" value="contig1.fasta.out" /> |
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93 <output name="twobit_output" file="contig1.both.2bit" /> |
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94 </test> |
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95 <test> |
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96 <!-- Test ignore pre-existing masking --> |
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97 <param name="twobit_input" value="contig1.bed.2bit" ftype="twobit" /> |
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98 <param name="maskfile_type_selector" value="out" /> |
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99 <param name="out_maskfile" value="contig1.fasta.out" /> |
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100 <param name="add_mask" value="" /> |
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101 <output name="twobit_output" file="contig1.out.2bit" /> |
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102 </test> |
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103 </tests> |
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104 <help> |
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105 <![CDATA[ |
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106 **What it does** |
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107 |
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108 twoBitMask applies a mask to a twoBit file based on repeat information |
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109 from a `RepeatMasker <http://www.repeatmasker.org/>`_ .out file or |
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110 a BED file (e.g., from |
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111 `Tandem Repeats Finder <https://tandem.bu.edu/trf/trf.html>`_). |
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112 |
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113 ]]></help> |
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114 |
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115 <expand macro="citations" /> |
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116 </tool> |