Mercurial > repos > yating-l > ucsc_blat
diff blat.xml @ 6:250a7f2b1331 draft
planemo upload commit 630d4a316c3b72b0d92b32e8b37303d247a41190
author | iuc |
---|---|
date | Thu, 31 May 2018 11:25:53 -0400 |
parents | dede60f744f4 |
children | 10f3a5b6f2ac |
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--- a/blat.xml Fri Jun 23 23:33:09 2017 -0400 +++ b/blat.xml Thu May 31 11:25:53 2018 -0400 @@ -1,8 +1,8 @@ <?xml version="1.0"?> -<tool id="ucsc_blat" name="UCSC BLAT Alignment Tool" version="35-0"> +<tool id="ucsc_blat" name="UCSC BLAT Alignment Tool" version="36"> <description>BLAST-like sequence alignment tool</description> <requirements> - <requirement type="package" version="35">blat</requirement> + <requirement type="package" version="36">blat</requirement> </requirements> <command detect_errors="exit_code"><![CDATA[ #if str($reference_source.reference_source_selector) == "history": @@ -20,25 +20,51 @@ #set $reference_fasta_filename = str($reference_source.database.fields.path) #end if - blat - #if $noHead == "yes" - -noHead - #end if + blat -q=$query_type -t=$database_type + ${oneOff} + + #if str($minScore) + -minScore=${minScore} + #end if + + -maxGap=${maxGap} + + #if str($repMatch) + -repMatch=${repMatch} + #end if + #if $mask_type.mask == "file.out": -mask='${mask_type.mask_file}' #else: -mask=$mask_type.mask #end if + #if str($dots) + -dots=${dots} + #end if + + ${trimT} + ${noTrimA} + ${trimHardA} + ${fastMap} + ${fine} + + #if str($maxIntron) + -maxIntron=${maxIntron} + #end if + + ${extendThroughN} + '$reference_fasta_filename' '${query}' - output - && sort -k 10,10 -k 12,12n output > '${output_sorted}' + -out=${out} + + '${output}' ]]></command> <inputs> <conditional name="reference_source"> @@ -71,10 +97,10 @@ <option value="dnax">dnax - DNA sequence translated in six frames to protein</option> <option value="rnax" selected="true">rnax - DNA sequence translated in three frames to protein</option> </param> - <param type="select" name="noHead" format="text" label="Suppresses .psl header (so it's just a tab-separated file)." > - <option value="yes" selected="true">Yes, suppresses .psl header</option> - <option value="no">No, do not suppresses .psl header</option> - </param> + <param argument="-oneOff" type="boolean" truevalue="-oneOff=1" falsevalue="" label="If set, this allows one mismatch in tile and still triggers an alignments" /> + <param argument="-minScore" type="integer" value="30" label="Minimum score" help="It is the matches minus the mismatches minus some sort of gap penalty" /> + <param argument="-maxGap" type="integer" value="2" min="0" max="3" label="Maximum gap between tiles in a clump" help="Usually set from 0 to 3. Only relevant for minMatch > 1" /> + <param argument="-repMatch" type="integer" value="" optional="true" label="Number of repetitions of a tile allowed before it is marked as overused" help="Typically this is 256 for tileSize 12, 1024 for tileSize 11, 4096 for tileSize 10. Also affected by stepSize. When stepSize is halved repMatch is doubled to compensate" /> <conditional name="mask_type"> <param name="mask" type="select" label="Mask out repeats" help="Alignments won't be started in masked region but may extend through it in nucleotide searches. Masked areas @@ -89,11 +115,39 @@ <when value="out" /> <when value="file.out"> <param name="mask_file" type="data" format="txt" label="RepeatMasker file.out" /> - </when> + </when> </conditional> + <param argument="-dots" type="integer" value="" optional="true" label="Output a dot every N sequences in log" help="Dots show program's progress" /> + <param argument="-trimT" type="boolean" truevalue="-trimT" falsevalue="" label="Trim leading poly-T" /> + <param argument="-noTrimA" type="boolean" truevalue="-noTrimA" falsevalue="" label="Don't trim trailing poly-A" /> + <param argument="-trimHardA" type="boolean" truevalue="-trimHardA" falsevalue="" label="Remove poly-A tail from qSize and alignments in .psl output" /> + <param argument="-fastMap" type="boolean" truevalue="-fastMap" falsevalue="" label="Run for fast DNA/DNA remapping" help="It does not allow introns and require high %ID. Query sizes must not exceed 5000" /> + <param argument="-fine" type="boolean" truevalue="-fine" falsevalue="" label="Refine search for small initial and terminal exons" help="For high-quality mRNAs. Not recommended for ESTs" /> + <param argument="-maxIntron" type="integer" value="750000" optional="true" label="Maximum intron size" /> + <param argument="-extendThroughN" type="boolean" truevalue="-extendThroughN" falsevalue="" label="Allow extension of alignment through large blocks of N's" /> + <param name="out" type="select" label="Select output file format (-out)"> + <option value="psl">Tab-separated format, no sequence (psl)</option> + <option value="psl -noHead">Tab-separated format, no sequence, no header (psl -noHead)</option> + <option value="axt">Blastz-associated axt format (axt)</option> + <option value="maf">Multiz-associated maf format (maf)</option> + <option value="sim4">Similar to sim4 format (sim4)</option> + <option value="wublast">Similar to WU-BLAST format (wublast)</option> + <option value="blast">Similar to NCBI BLAST format (blast)</option> + <option value="blast8">NCBI BLAST tabular format (blast8)</option> + <option value="blast9">NCBI BLAST tabular format with comments (blast9)</option> + </param> </inputs> <outputs> - <data format="psl" name="output_sorted" label="${tool.name} on ${on_string}"></data> + <data name="output" format="tabular" label="${tool.name} on ${on_string}"> + <change_format> + <when input="out" value="psl" format="psl" /> + <when input="out" value="axt" format="axt" /> + <when input="out" value="maf" format="maf" /> + <when input="out" value="sim4" format="txt" /> + <when input="out" value="wublast" format="txt" /> + <when input="out" value="blast" format="txt" /> + </change_format> + </data> </outputs> <tests> <!-- test on query of GenBank RefSeq records for Gallus gallus and database of Amazona vittata --> @@ -101,44 +155,53 @@ <param name="reference_source_selector" value="history" /> <param name="database" value="amaVit1_Gallus/amaVit1.fa" /> <param name="query" value="amaVit1_Gallus/Gallus_gallus_RefSeq.fa" /> - <param name="database_type" value="dnax" /> - <param name="query_type" value="rnax" /> - <param name="noHead" value="yes" /> + <param name="database_type" value="dnax" /> + <param name="query_type" value="rnax" /> <param name="mask" value="lower" /> - <output name="output_sorted" value="amaVit1_Gallus/amaVit1_Gallus_gallus.psl" /> + <param name="out" value="psl -noHead" /> + <output name="output" value="amaVit1_Gallus/amaVit1_Gallus_gallus_sorted.psl" sort="True"/> </test> <!-- test on query of partial mRNA of Drosophila melanogaster and the database of Drosophila biamipes dot chromosome --> <test> <param name="reference_source_selector" value="history" /> <param name="database" value="dbia3/dbia3.fa" /> <param name="query" value="dbia3/dmel-transcript.fa" /> - <param name="database_type" value="dnax" /> - <param name="query_type" value="rnax" /> - <param name="noHead" value="yes" /> + <param name="database_type" value="dnax" /> + <param name="query_type" value="rnax" /> <param name="mask" value="lower" /> - <output name="output_sorted" value="dbia3/dbia3.sorted.psl" /> + <param name="out" value="psl -noHead" /> + <param name="maxIntron" value="" /> + <output name="output" value="dbia3/dbia3.sorted.psl" sort="True"/> </test> <!-- test on the database masked by repeat masker --> <test> <param name="reference_source_selector" value="history" /> <param name="database" value="dbia3/dbia3_masked.2bit" /> <param name="query" value="dbia3/dmel-transcript.fa" /> - <param name="database_type" value="dnax" /> - <param name="query_type" value="rnax" /> - <param name="noHead" value="yes" /> + <param name="database_type" value="dnax" /> + <param name="query_type" value="rnax" /> + <param name="oneOff" value="false" /> + <param name="minScore" value="30" /> + <param name="maxGap" value="2" /> + <param name="trimT" value="false" /> + <param name="noTrimA" value="false" /> + <param name="fine" value="false" /> + <param name="maxIntron" value="750000" /> + <param name="extendThroughN" value="false" /> <param name="mask" value="file.out" /> <param name="mask_file" value="dbia3/dbia3_RM.out" /> - <output name="output_sorted" value="dbia3/dbia3_masked.sorted.psl" /> + <param name="out" value="psl -noHead" /> + <output name="output" value="dbia3/dbia3_masked.sorted.psl"/> </test> - </tests> + </tests> <help> <![CDATA[ BLAT ==== -BLAT is a bioinformatics software a tool which performs rapid mRNA/DNA and cross-species protein alignments. +BLAT is a bioinformatics software a tool which performs rapid mRNA/DNA and cross-species protein alignments. -blat (version: v340)- Standalone blat sequence search command line tool. +blat (version: v36)- Standalone blat sequence search command line tool. ------------------------------------------------------------------------- usage: @@ -152,25 +215,20 @@ -ooc=11.ooc tells the program to load over-occurring 11-mers from an external file. This will increase the speed by a factor of 40 in many cases, but is not required. - output.psl is the name of the output file. + output.psl is the name of the output file. documentation: ++++++++++++++ -See Blat documentation (http://genome.ucsc.edu/goldenPath/help/blatSpec.html) +See Blat documentation (http://genome.ucsc.edu/goldenPath/help/blatSpec.html) Source code: ++++++++++++ http://hgdownload.cse.ucsc.edu/admin/exe/ -]]></help> +]]></help> <citations> <citation type="doi">10.1101/gr.229202</citation> -</citations> +</citations> </tool> - - - - -