Mercurial > repos > yhoogstrate > flaimapper
annotate flaimapper.xml @ 8:4f22bd612754 draft
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
author | erasmus-medical-center |
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date | Wed, 16 Aug 2017 11:07:51 -0400 |
parents | 0ea90514e0a0 |
children | 0addbdbc1344 |
rev | line source |
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0
d6abffbc9ee7
planemo upload for repository https://bitbucket.org/EMCbioinf/galaxy-tool-shed-tools/raw/master/flaimapper commit 0f4aa594becc89b07073f7fcccd889e38974e10c-dirty
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1 <?xml version="1.0" encoding="UTF-8"?> |
8
4f22bd612754
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
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2 <tool id="flaimapper" name="FlaiMapper" version="2.5.0-0"> |
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c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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3 <description>detects small ncRNA derived fragments in small RNA-Seq data</description> |
3 | 4 <requirements> |
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4f22bd612754
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
erasmus-medical-center
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5 <requirement type="package" version="2.5.0">flaimapper</requirement> |
3 | 6 </requirements> |
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4f22bd612754
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
erasmus-medical-center
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7 |
4f22bd612754
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
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8 <version_command><![CDATA[ flaimapper --version 2>&1 | head -n 1 ]]></version_command> |
4f22bd612754
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
erasmus-medical-center
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9 |
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c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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10 <command detect_errors="exit_code"><![CDATA[ |
3 | 11 flaimapper |
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c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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12 -v |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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13 -f '${output_select.output_format}' |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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14 -o '${output}' |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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15 |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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16 #if $output_select.output_format == '1': |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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17 #if $output_select.fasta: |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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18 -r '${output_select.fasta}' |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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19 #end if |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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20 #else |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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21 --offset5p ${output_select.offset5p} |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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22 --offset3p ${output_select.offset3p} |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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23 #end if |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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24 |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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25 #if $parameters: |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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26 -p '${parameters}' |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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27 #end if |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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28 |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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29 '${alignment}' |
3 | 30 ]]></command> |
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4f22bd612754
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
erasmus-medical-center
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31 |
3 | 32 <inputs> |
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c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
yhoogstrate
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33 <param name="alignment" type="data" format="bam" multiple="false" |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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34 label="Alignment file" |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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35 help="Aligned small RNA-Seq reads must be single end and should not be fragmented in the library preparation" /> |
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4f22bd612754
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
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36 |
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c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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37 <conditional name="output_select"> |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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38 <param name="output_format" type="select" label="Output format" argument="-f"> |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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39 <option value="1">Tabular</option> |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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40 <option value="2">GTF</option> |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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41 </param> |
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4f22bd612754
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
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42 |
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c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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43 <when value="1"> |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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44 <param name="fasta" type="data" format="fasta" optional="true" |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit beb30713f9b8510c22f46d94cb58c92cf26c799c
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45 label="(Optional) Genome reference in FASTA format that corresponds to the reference genome or RNA database" |
0ea90514e0a0
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit beb30713f9b8510c22f46d94cb58c92cf26c799c
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46 help="By selecting this file, sequences will be provided in the corresponding column in the output file" argument="-r" /> |
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c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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47 </when> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
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48 |
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c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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49 <when value="2"> |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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50 <param name="offset5p" type="integer" value="4" |
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0ea90514e0a0
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit beb30713f9b8510c22f46d94cb58c92cf26c799c
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51 label="Add 5'-offset to exon-type entry in GTF output file" |
0ea90514e0a0
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit beb30713f9b8510c22f46d94cb58c92cf26c799c
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52 help="Exon-type entries are often used to measure expression. Because of the small size of the fragments it may be desired to add an offset to allow less stringent read counting." argument="--offset5p" /> |
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c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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53 <param name="offset3p" type="integer" value="4" |
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0ea90514e0a0
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit beb30713f9b8510c22f46d94cb58c92cf26c799c
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54 label="Add 3'-offset to exon-type entry in GTF output file" |
0ea90514e0a0
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit beb30713f9b8510c22f46d94cb58c92cf26c799c
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55 help="Exon-type entries are often used to measure expression. Because of the small size of the fragments it may be desired to add an offset to allow less stringent read counting." argument="--offset3p" /> |
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c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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56 </when> |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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57 </conditional> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
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58 |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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59 <param name="parameters" type="data" format="txt,tabular" optional="true" |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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60 label="(Optional) Custom parameters file" help="" argument="-p" /> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
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61 |
3 | 62 </inputs> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
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63 |
3 | 64 <outputs> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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65 <data format="tabular" name="output" |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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66 label="${tool.name} on $on_string"> |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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67 <change_format> |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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68 <when input="output_select.output_format" value="1" format="tabular" /> |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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69 <when input="output_select.output_format" value="2" format="gtf" /> |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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70 </change_format> |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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71 </data> |
3 | 72 </outputs> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
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73 |
3 | 74 <tests> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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75 <!-- tabular --> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
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76 <!-- Testing "ncRNAdb09 alignment"-type analysis --> |
4f22bd612754
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
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77 <test> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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78 <param name="alignment" value="snord81.bam" ftype="bam" /> |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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79 <param name="fasta" value="snord81.fa" ftype="fasta" /> |
c143e7e1fdbf
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80 <param name="output_format" value="1" /> |
8
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81 |
6
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82 <output name="output" file="snord81.flaimapper.txt" /> |
c143e7e1fdbf
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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83 </test> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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84 <test> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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85 <param name="alignment" value="snord81.bam" ftype="bam" /> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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86 <param name="output_format" value="1" /> |
8
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
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87 |
6
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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88 <output name="output" file="snord81.flaimapper.no-seq.txt" /> |
3 | 89 </test> |
8
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
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90 <!-- Testing "Full genome alignment"-type analysis --> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit ad77d5da986c4cd976cd5de7abcdc91c6dd505ad
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91 <test> |
6
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92 <param name="alignment" value="test_genomic_alignment.bam" ftype="bam" /> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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93 <param name="fasta" value="test_genomic_all_chromosomes.fa" ftype="fasta" /> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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94 <param name="output_format" value="1" /> |
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95 |
6
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96 <output name="output" file="test_genomic_flaimapper_output.txt" /> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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97 </test> |
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98 <test> |
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99 <param name="alignment" value="test_genomic_alignment.bam" ftype="bam" /> |
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planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/flaimapper commit 0c5344c3f9c398cdcc18f6bb61681afe48f0c306
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100 <param name="output_format" value="1" /> |
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101 |
6
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102 <output name="output" file="test_genomic_flaimapper_output.no-seq.txt" /> |
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103 </test> |
8
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104 |
6
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105 <!-- GTF --> |
8
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106 <!-- Testing "ncRNAdb09 alignment"-type analysis --> |
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107 <test> |
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108 <param name="alignment" value="snord81.bam" ftype="bam" /> |
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109 <param name="output_format" value="2" /> |
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110 |
6
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111 <output name="output" file="snord81.flaimapper.gtf" /> |
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112 </test> |
8
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113 <!-- Testing "Full genome alignment"-type analysis --> |
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114 <test> |
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115 <param name="alignment" value="test_genomic_alignment.bam" ftype="bam" /> |
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116 <param name="output_format" value="2" /> |
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117 |
6
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118 <output name="output" file="test_genomic_flaimapper_output.gtf" /> |
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119 </test> |
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120 |
6
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121 <!-- test custom parameters --> |
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122 <test> |
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123 <param name="alignment" value="snord81.bam" ftype="bam" /> |
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124 <param name="output_format" value="2" /> |
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125 <param name="offset5p" value="5" /> |
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126 <param name="offset3p" value="5" /> |
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127 |
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128 <output name="output" file="snord81.flaimapper.offsets_5_5.gtf" /> |
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129 </test> |
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130 <test> |
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131 <param name="alignment" value="snord81.bam" ftype="bam" /> |
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132 <param name="output_format" value="2" /> |
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133 <param name="parameters" value="filter-parameters.duck.15.txt"/> |
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134 |
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135 <output name="output" file="snord81.flaimapper.duck-15.gtf" /> |
3 | 136 </test> |
137 </tests> | |
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138 |
3 | 139 <help><![CDATA[ |
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140 FlaiMapper wrapper for Galaxy |
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141 ============================= |
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142 |
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143 Fragment Location Annotation Identification Mapper |
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144 |
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145 FlaiMapper: computational annotation of small ncRNA-derived fragments using RNA-seq high-throughput data. |
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146 |
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147 |
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148 Input |
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149 ----- |
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150 |
6
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151 Alignment |
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152 ********* |
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153 |
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154 This file has to contain aligned single end reads from a small RNA-Seq experiment, provided in the BAM format. |
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155 |
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156 Prior to running FlaiMapper, it is common to align sequencing reads to either: |
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157 |
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158 - mature ncRNA sequences |
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159 - all chromosomes |
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160 |
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161 Example- and reference data |
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162 *************************** |
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163 |
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164 The reference sequence should be provided in FASTA format. |
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165 |
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166 You can access **ncRNAdb09** FASTA file at the following URL: |
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167 https://raw.githubusercontent.com/yhoogstrate/flaimapper/master/share/annotations/ncRNA_annotation/ncrnadb09.fa *(reference file)* |
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168 |
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169 If you want to test FlaiMapper with example data you can obtain several |
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170 alignment files from the following directory tree: |
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171 |
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172 https://github.com/yhoogstrate/flaimapper/tree/master/share/small_RNA-seq_alignments |
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173 |
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174 More details are given in the manual at the following website: |
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175 |
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176 https://github.com/yhoogstrate/flaimapper |
3 | 177 ]]></help> |
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178 |
3 | 179 <citations> |
180 <citation type="doi">10.1093/bioinformatics/btu696</citation> | |
181 </citations> | |
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182 </tool> |