Mercurial > repos > yhoogstrate > fuma
annotate fuma.xml @ 3:4966079d474b draft
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2-dirty
author | yhoogstrate |
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date | Tue, 20 Oct 2015 10:12:08 -0400 |
parents | 86526900cb8f |
children | cb0543909e83 |
rev | line source |
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0
a4cfaa0e3e5d
planemo upload for repository https://bitbucket.org/EMCbioinf/galaxy-tool-shed-tools/raw/master/fuma commit f56125b28ec44aa28943ed040b7b202fed9c875b-dirty
yhoogstrate
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1 <?xml version="1.0" encoding="UTF-8"?> |
2
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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2 <tool id="fuma" name="FuMa" version="2.10.0.a"> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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3 <description>match detected fusion genes based on gene names (in particular for RNA-Seq).</description> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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4 |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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5 <requirements> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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6 <requirement type="package" version="2.7">python</requirement> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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7 <requirement type="package" version="2.10.0">fuma</requirement> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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8 </requirements> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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9 |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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10 <version_command>fuma --version 2>&1 | head -n 1</version_command><!-- -V also works, but is not GNU standard --> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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11 |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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12 <command><![CDATA[ |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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13 #import pipes |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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14 |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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15 #set $gene_annotations = [] |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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16 #set $samples = [] |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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17 #set $links = [] |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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18 |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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19 #for $i, $d in enumerate( $datasets ) |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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20 #set $sample_name = pipes.quote(str($d['sample'].name)) |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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21 |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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22 #set $gene_annotations = $gene_annotations + [ "ga_" + str($i) + ":" + str($d['gene_annotation'].file_name) ] |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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23 |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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24 #set $samples = $samples + [ $sample_name + ":" + str($d['format']) + ":" + str($d['sample'].file_name) ] |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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25 #set $links = $links + [ $sample_name + ":" + str("ga_") + str($i) ] |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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26 #end for |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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27 |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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28 #set $gene_annotations_str = " ".join(gene_annotations) |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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29 #set $samples_str = " ".join(samples) |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
parents:
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30 #set $links_str = " ".join(links) |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
parents:
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31 |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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32 fuma |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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33 -m |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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34 $params.matching_method |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
parents:
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35 |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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36 $params.strand_specific_matching |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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37 $params.acceptor_donor_order_specific_matchig |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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38 |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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39 -a |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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40 $gene_annotations_str |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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41 -s |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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42 $samples_str |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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43 -l |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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44 $links_str |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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45 #if $params.output_format.value == "list_boolean" |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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46 -f list |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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47 #else |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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48 -f $params.output_format.value |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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49 #end if |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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50 -o $fuma_overview ; |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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51 |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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52 |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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53 #if $params.output_format.value == "list_boolean" |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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54 fuma-list-to-boolean-list -o tmp.txt $fuma_overview ; |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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55 mv tmp.txt $fuma_overview |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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56 #end if |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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57 ]]></command> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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58 |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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59 <inputs> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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60 <repeat name="datasets" title="FusionGene Datasets" min="2"> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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61 <param name="sample" type="data" format="txt,tabular" label="Dataset (RNA-Seq fusion gene detection experiment)" /> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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62 <param name="format" type="select" label="Format of dataset"> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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63 <option value="chimera">Chimera prettyPrint()</option> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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64 <option value="chimerascan">ChimeraScan</option> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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65 <option value="defuse">DeFuse</option> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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66 <option value="complete-genomics">Complete Genomics var/mastervar</option> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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67 <option value="fusion-catcher_final">Fusion Catcher (final-list file)</option> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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68 <option value="fusionmap">FusionMap</option> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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69 <option value="trinity-gmap">GMAP (As step after Trinity)</option> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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70 <option value="oncofuse">OncoFuse</option> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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71 <option value="rna-star_chimeric">STAR (chimeric file)</option> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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72 <option value="star-fusion_final">STAR-Fusion (candidates.final)</option> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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73 <option value="tophat-fusion_pre">Tophat Fusion Pre (fusions.out)</option> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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74 <option value="tophat-fusion_post_potential_fusion">Tophat Fusion Post (potential_fusion.txt)</option> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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75 <option value="tophat-fusion_post_result">Tophat Fusion Post (result.txt)</option> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
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76 <option value="tophat-fusion_post_result_html">Tophat Fusion Post (result.html)</option> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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77 </param> |
86526900cb8f
planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit 3da3fcc0204205d4899763f9fe63edf9aa16d5a2
yhoogstrate
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78 <param name="gene_annotation" type="data" format="bed" label="Corresponding gene-name annotation file (BED format)" help="Make use of persistent gene annotations! Gene annotations should only be different if different reference genome builds were used." /> |
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79 </repeat> |
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80 |
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81 <conditional name="params"> |
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82 <param name="settingsType" type="select" label="Settings to use" help="You can use the default settings or set custom values for any FuMa parameter."> |
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83 <option value="preSet" selected="true">Use Defaults</option> |
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84 <option value="full">Full parameter list</option> |
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85 </param> |
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86 <when value="preSet"> |
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87 <param name="strand_specific_matching" type="hidden" value="--strand-specific-matching" /> |
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88 <param name="acceptor_donor_order_specific_matchig" type="hidden" value="--acceptor-donor-order-specific-matching" /> |
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89 </when> |
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90 <when value="full"> |
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91 <param name="matching_method" type="select" label="Matching method: technique used to match fusion genes based on annotated gene sets" help="Overlap is the most sensitive but also more sensitive for long gene artefacts; subset is the recommended technique and EGM is conservative."> |
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92 <option value="overlap">Overlap</option> |
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93 <option value="subset" selected="True">Subset</option> |
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94 <option value="egm">Exact Geneset Matching (EGM)</option> |
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95 </param> |
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96 |
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97 <param name="strand_specific_matching" type="boolean" checked="True" truevalue="--strand-specific-matching" falsevalue="" label="Consider fusion genes distinct when the breakpoints have different strands" help="Only a limited number of file formats support this feature." /> |
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98 <param name="acceptor_donor_order_specific_matchig" type="boolean" checked="True" truevalue="--acceptor-donor-order-specific-matching" falsevalue="" label="Consider fusion genes distinct when the donor and acceptor sites are swapped (A,B) != (B,A)" help="This settings is not recommended when fusion genes detected in DNA-Seq are used" /> |
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99 |
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100 <param name="output_format" type="select" label="Output format"> |
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101 <option value="list_boolean" selected="true">List (Boolean)</option> |
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102 <option value="list">List</option> |
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103 <option value="summary">Count summary</option> |
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104 </param> |
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105 </when> |
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106 </conditional> |
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107 </inputs> |
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108 |
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109 <outputs> |
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110 <data format="tabular" name="fuma_overview" label="${tool.name} on ${', '.join([ str(d['sample'].hid)+': '+d['sample'].name for d in $datasets ])}" /> |
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111 </outputs> |
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112 |
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113 <tests> |
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114 <test> |
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115 <!-- <repeat name="datasets"> --> |
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116 <param name="datasets_0|sample" value="edgren_chimerascan.txt" ftype="tabular" /> |
2
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117 <param name="datasets_0|format" value="chimerascan" /> |
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118 <param name="datasets_0|gene_annotation" value="refseq_genes_hg19.bed" ftype="bed" /> |
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119 <!-- </repeat> --> |
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120 <!-- <repeat name="datasets"> --> |
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121 <param name="datasets_1|sample" value="edgren_defuse.txt" ftype="tabular" /> |
2
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122 <param name="datasets_1|format" value="defuse" /> |
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123 <param name="datasets_1|gene_annotation" value="refseq_genes_hg19.bed" ftype="bed" /> |
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124 <!-- </repeat> --> |
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125 <!-- <repeat name="datasets"> --> |
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126 <param name="datasets_2|sample" value="edgren_fusion-map.txt" ftype="tabular" /> |
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127 <param name="datasets_2|format" value="fusionmap" /> |
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128 <param name="datasets_2|gene_annotation" value="refseq_genes_hg19.bed" ftype="bed" /> |
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129 <!-- </repeat> --> |
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130 <!-- <repeat name="datasets"> --> |
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131 <param name="datasets_3|sample" value="edgren_true_positives.txt" ftype="tabular" /> |
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132 <param name="datasets_3|format" value="fusionmap" /> |
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133 <param name="datasets_3|gene_annotation" value="refseq_genes_hg19.bed" ftype="bed" /> |
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134 <!-- </repeat> --> |
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135 |
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136 <param name="settingsType" value="full" /> |
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137 |
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138 <param name="matching_method" value="subset" /> |
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139 <param name="strand_specific_matching" value="--strand-specific-matching" /> |
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140 <param name="acceptor_donor_order_specific_matchig" value="--acceptor-donor-order-specific-matching" /> |
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141 <param name="output_format" value="list_boolean" /> |
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142 |
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143 <output name="fuma_overview" file="edgren_test_01_specifc_matching_output.txt" /> |
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144 </test> |
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145 <test> |
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146 <!-- <repeat name="datasets"> --> |
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147 <param name="datasets_0|sample" value="edgren_chimerascan.txt" ftype="tabular" /> |
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148 <param name="datasets_0|format" value="chimerascan" /> |
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149 <param name="datasets_0|gene_annotation" value="refseq_genes_hg19.bed" ftype="bed" /> |
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150 <!-- </repeat> --> |
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151 <!-- <repeat name="datasets"> --> |
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152 <param name="datasets_1|sample" value="edgren_defuse.txt" ftype="tabular" /> |
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153 <param name="datasets_1|format" value="defuse" /> |
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154 <param name="datasets_1|gene_annotation" value="refseq_genes_hg19.bed" ftype="bed" /> |
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155 <!-- </repeat> --> |
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156 <!-- <repeat name="datasets"> --> |
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157 <param name="datasets_2|sample" value="edgren_fusion-map.txt" ftype="tabular" /> |
2
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158 <param name="datasets_2|format" value="fusionmap" /> |
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159 <param name="datasets_2|gene_annotation" value="refseq_genes_hg19.bed" ftype="bed" /> |
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160 <!-- </repeat> --> |
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161 <!-- <repeat name="datasets"> --> |
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162 <param name="datasets_3|sample" value="edgren_true_positives.txt" ftype="tabular" /> |
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163 <param name="datasets_3|format" value="fusionmap" /> |
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164 <param name="datasets_3|gene_annotation" value="refseq_genes_hg19.bed" ftype="bed" /> |
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165 <!-- </repeat> --> |
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166 |
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167 <param name="settingsType" value="full" /> |
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168 |
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169 <param name="matching_method" value="subset" /> |
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170 <param name="strand_specific_matching" value="" /> |
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171 <param name="acceptor_donor_order_specific_matchig" value="" /> |
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172 <param name="output_format" value="list_boolean" /> |
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173 |
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174 <output name="fuma_overview" file="edgren_test_02_unspecifc_matching_output.txt" /> |
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175 </test> |
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176 </tests> |
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177 |
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178 <help><![CDATA[ |
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179 ============ |
0
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180 Introduction |
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181 ============ |
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182 |
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183 FuMa (Fusion Matcher) matches predicted fusion events (both genomic and transcriptomic) according to chromosomal location or assocatiated gene annotation(s) where the latter should be genome build inspecific. |
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184 |
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185 Because RNA-Sequencing deals with samples that may have undergrond splicing, reads may split up because of biological processes. If a fusion event takes place, the same thing may happen. Therefore we hypothesize that using spanning read distances may be unreliable, because there are known introns of > 100kb. Therefore, FuMa translates the breakpoint to gene names, and only overlaps breakpoints with the same genename(s). |
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186 |
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187 ===== |
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188 Usage |
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189 ===== |
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190 |
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191 After you have uploaded the results of your Fusion Gene detection experiment, and selected the format to be *tabular*, you can start the FuMa wrapper. For each dataset you simply have to add another repeat. Then you have to select a corresponding format: |
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192 |
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193 ******* |
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194 Formats |
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195 ******* |
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196 |
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197 +-------------------+-----------------------+-------------------------------------+ |
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198 |Tools | File | Format string | |
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199 +===================+=======================+=====================================+ |
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200 |Chimera | prettyPrint() output | chimera | |
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201 +-------------------+-----------------------+-------------------------------------+ |
0
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202 |ChimeraScan | chimeras.bedpe | chimerascan | |
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203 +-------------------+-----------------------+-------------------------------------+ |
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204 |Complete Genomics | highConfidenceJu*.tsv | complete-genomics | |
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205 +-------------------+-----------------------+-------------------------------------+ |
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206 |Complete Genomics | allJunctionsBeta*.tsv | complete-genomics | |
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207 +-------------------+-----------------------+-------------------------------------+ |
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208 |DeFuse | results.txt | defuse | |
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209 +-------------------+-----------------------+-------------------------------------+ |
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210 |DeFuse | results.classify.txt | defuse | |
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211 +-------------------+-----------------------+-------------------------------------+ |
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212 |DeFuse | results.filtered.txt | defuse | |
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213 +-------------------+-----------------------+-------------------------------------+ |
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214 |Fusion Catcher | final-list_cand*.txt | fusion-catcher_final | |
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215 +-------------------+-----------------------+-------------------------------------+ |
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216 |FusionMap | | fusionmap | |
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217 +-------------------+-----------------------+-------------------------------------+ |
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218 |Trinity + GMAP | | trinity-gmap | |
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219 +-------------------+-----------------------+-------------------------------------+ |
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220 |OncoFuse | | oncofuse | |
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221 +-------------------+-----------------------+-------------------------------------+ |
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222 |RNA STAR | Chimeric.out.junction | rna-star_chimeric | |
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223 +-------------------+-----------------------+-------------------------------------+ |
2
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224 |STAR Fusion | _candidates.final | star-fusion_final | |
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225 +-------------------+-----------------------+-------------------------------------+ |
0
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226 |TopHat Fusion pre | fusions.out | tophat-fusion_pre | |
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227 +-------------------+-----------------------+-------------------------------------+ |
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228 |TopHat Fusion post | potential_fusion.txt | tophat-fusion_post_potential_fusion | |
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229 +-------------------+-----------------------+-------------------------------------+ |
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230 |TopHat Fusion post | result.txt | tophat-fusion_post_result | |
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231 +-------------------+-----------------------+-------------------------------------+ |
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232 |TopHat Fusion post | result.html | tophat-fusion_post_result_html | |
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233 +-------------------+-----------------------+-------------------------------------+ |
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234 |
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235 To annotate genes upon the breakpoints you must provide a BED file that contains gene annotations for the user genome build. Make sure **your BED file contains one gene per line**. You should use BED files that contain one exon per line only if you want restrict your analysis to fusion genes detected within exons. |
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236 |
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237 UCSC genome browser provides a very simple way of obtaining BED files with one gene per line by selecting their *RefSeq Genes*-track and *knownGene*-table and putting the export format to BED. Galaxy should have a built-in UCSC table browser. |
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238 |
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239 ]]></help> |
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240 |
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241 <citations> |
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242 <citation type="bibtex"> |
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243 @unpublished{fuma, |
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244 author = {Youri Hoogstrate}, |
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245 title = {FuMa: reporting overlap in RNA-seq detected fusion genes}, |
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246 url = { https://github.com/yhoogstrate/fuma } |
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247 } |
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248 </citation> |
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249 </citations> |
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250 </tool> |