comparison plot_distribution.r @ 0:f91478b63ec6 draft

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author ynewton
date Thu, 13 Dec 2012 11:25:09 -0500
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children cf8d0d54bc78
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1 #!/usr/bin/Rscript
2
3 #usage, options and doc goes here
4 argspec <- c("normalize.r - takes any flat file and normalizes the rows or the columns using various normalizations (median_shift, mean_shift, t_statistic (z-score), exp_fit, normal_fit, weibull_0.5_fit, weibull_1_fit, weibull_1.5_fit, weibull_5_fit). Requires a single header line and a single cloumn of annotation.
5 Usage:
6 normalize.r input.tab norm_type norm_by > output.tab
7 Example:
8 Rscript normalize.r test_matrix.tab median_shift column > output.tab
9 Rscript normalize.r test_matrix.tab mean_shift row normals.tab > output.tab
10 Options:
11 input matrix (annotated by row and column names)
12 normalization type; available options:
13 median_shift - shifts all values by the median or the row/column if no normals are specified, otherwise shifts by the median of normals
14 mean_shift - shifts all values by the mean or the row/column if no normals are specified, otherwise shifts by the mean of normals
15 t_statistic - converts all values to z-scores; if normals are specified then converts to z-scores within normal and non-normal classes separately
16 exp_fit - (only by column) ranks data and transforms exponential CDF
17 normal_fit - (only by column) ranks data and transforms normal CDF
18 weibull_0.5_fit - (only by column) ranks data and transforms Weibull CDF with scale parameter = 1 and shape parameter = 0.5
19 weibull_1_fit - (only by column) ranks data and transforms Weibull CDF with scale parameter = 1 and shape parameter = 1
20 weibull_1.5_fit - (only by column) ranks data and transforms Weibull CDF with scale parameter = 1 and shape parameter = 1.5
21 weibull_5_fit - (only by column) ranks data and transforms Weibull CDF with scale parameter = 1 and shape parameter = 5
22 normalization by:
23 row
24 column
25 normals_file is an optional parameter which contains a list of column headers from the input matrix, which should be considered as normals
26 output file is specified through redirect character >")
27
28 read_matrix <- function(in_file){
29 header <- strsplit(readLines(con=in_file, n=1), "\t")[[1]]
30 cl.cols<- 1:length(header) > 1
31 data_matrix.df <- read.delim(in_file, header=TRUE, row.names=NULL, stringsAsFactors=FALSE, na.strings="NA", check.names=FALSE)
32 data_matrix <- as.matrix(data_matrix.df[,cl.cols])
33 rownames(data_matrix) <- data_matrix.df[,1]
34 return(data_matrix)
35 }
36
37 main <- function(argv) {
38 in_file <- argv[1]
39 out_file <- argv[2]
40 sink('/dev/null')
41
42 input_data <- read_matrix(in_file)
43
44 pdf(out_file, bg="white")
45 par(mfrow=c(1,1))
46 hist(input_data, col="lightblue", labels=TRUE, main="Histogram", xlab="")
47 plot(density(input_data), type="l", col="blue", main="Density")
48 dev.off()
49 }
50
51 main(commandArgs(TRUE))