Mercurial > repos > yufei-luo > differential_expression_analysis_pipeline_for_rnaseq_data
diff DiffExpAnal/gsnap.xml @ 0:63799b789162 draft
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author | yufei-luo |
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date | Tue, 22 Jan 2013 10:07:03 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/DiffExpAnal/gsnap.xml Tue Jan 22 10:07:03 2013 -0500 @@ -0,0 +1,49 @@ +<tool id="gsnap" name="gsnap"> + + <description>GSNAP version 2012-12-20. + GMAP: A Genomic Mapping and Alignment Program for mRNA and EST Sequences, and + GSNAP: Genomic Short-read Nucleotide Alignment Program + </description> + + <requirements> + <requirement type="package">gmap</requirement> + </requirements> + + + <command interpreter="python"> wrappGSNAP.py + -d $genomeName -D $genomeName.files_path -k $kmer -i $inputFasta -q $inputFastq -A $outputFormat + + #if $optionPairedEnd.paire == 'Yes': + -p $optionPairedEnd.pairedEndFile + #end if + + > $outputSam + + </command> + + <inputs> + <param name="inputFasta" type="data" format="fasta" label="Reference genome file, fasta format."/> + <param name="genomeName" type="text" value="Arabidopsis_Thaliana" label="Please give the reference genome a name! (Ex. Arabidopsis_Thaliana)"/> + <param name="kmer" type="integer" value="12" label="Choose kmer value (<=16), a big kmer value can take more RAM(4Go)." /> + <param name="inputFastq" type="data" format="fastq" label="Input fastq file."/> + <param name="outputFormat" type="text" format="sam" label="Choose an output format [sam, goby (need to re-compile with appropriate options)]."/> + + <conditional name="optionPairedEnd"> + <param name="paire" type="select" label="pairedEnd fastq file"> + <option value="Yes">Yes</option> + <option value="No" selected="true">No</option> + </param> + <when value="Yes"> + <param name="pairedEndFile" type="data" format="fastq"/> + </when> + <when value="No"> + </when> + </conditional> + + </inputs> + + <outputs> + <data format="sam" name="outputSam" label="gsnap Output"/> + </outputs> + +</tool>