annotate commons/core/coord/Align.py @ 9:1eb55963fe39

Updated CompareOverlappingSmall*.py
author m-zytnicki
date Thu, 14 Mar 2013 05:23:05 -0400
parents 769e306b7933
children
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1 # Copyright INRA (Institut National de la Recherche Agronomique)
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2 # http://www.inra.fr
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3 # http://urgi.versailles.inra.fr
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4 #
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5 # This software is governed by the CeCILL license under French law and
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6 # abiding by the rules of distribution of free software. You can use,
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7 # modify and/ or redistribute the software under the terms of the CeCILL
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8 # license as circulated by CEA, CNRS and INRIA at the following URL
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9 # "http://www.cecill.info".
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10 #
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11 # As a counterpart to the access to the source code and rights to copy,
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12 # modify and redistribute granted by the license, users are provided only
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13 # with a limited warranty and the software's author, the holder of the
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14 # economic rights, and the successive licensors have only limited
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15 # liability.
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16 #
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17 # In this respect, the user's attention is drawn to the risks associated
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18 # with loading, using, modifying and/or developing or reproducing the
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19 # software by the user in light of its specific status of free software,
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20 # that may mean that it is complicated to manipulate, and that also
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21 # therefore means that it is reserved for developers and experienced
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22 # professionals having in-depth computer knowledge. Users are therefore
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23 # encouraged to load and test the software's suitability as regards their
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24 # requirements in conditions enabling the security of their systems and/or
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25 # data to be ensured and, more generally, to use and operate it in the
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26 # same conditions as regards security.
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27 #
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28 # The fact that you are presently reading this means that you have had
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29 # knowledge of the CeCILL license and that you accept its terms.
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30
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31 import time
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32
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33 from commons.core.coord.Range import Range
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34 from commons.core.coord.Map import Map
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35
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36
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37 ## Handle a match between two sequences, query and subject (pair of coordinates with E-value, score and identity)
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38 #
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39 class Align( object ):
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40
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41 ## Constructor
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42 #
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43 # @param range_q: a Range instance for the query
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44 # @param range_s: a Range instance for the subject
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45 # @param e_value: E-value of the match
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46 # @param identity: identity percentage of the match
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47 # @param score: score of the match
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48 #
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49 def __init__(self, range_q=Range(), range_s=Range(), e_value=0, score=0, identity=0):
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50 self.range_query = range_q
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51 self.range_subject = range_s
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52 self.e_value = float(e_value)
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53 self.score = float(score)
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54 self.identity = float(identity)
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55
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56 ## Return True if the instance is empty, False otherwise
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57 #
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58 def isEmpty(self):
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59 return self.range_query.isEmpty() or self.range_subject.isEmpty()
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60
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61 ## Equal operator
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62 #
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63 def __eq__(self, o):
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64 if self.range_query==o.range_query and self.range_subject==o.range_subject and \
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65 self.e_value==o.e_value and self.score==o.score and self.identity==o.identity:
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66 return True
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67 return False
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68
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69 ## Unequal operator
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70 #
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71 # @param o a Range instance
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72 #
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73 def __ne__(self, o):
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74 return not self.__eq__(o)
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75
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76 ## Convert the object into a string
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77 #
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78 # @note used in 'print myObject'
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79 #
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80 def __str__( self ):
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81 return self.toString()
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82
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83 ## Read attributes from an Align file
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84 #
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85 # @param fileHandler: file handler of the file being read
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86 # @return: 1 on success, 0 at the end of the file
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87 #
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88 def read(self, fileHandler):
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89 self.reset()
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90 line = fileHandler.readline()
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91 if line == "":
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92 return 0
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93 tokens = line.split("\t")
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94 if len(tokens) < len(self.__dict__.keys()):
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95 return 0
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96 self.setFromTuple(tokens)
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97 return 1
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98
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99 ## Set attributes from tuple
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100 #
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101 # @param tuple a tuple with (queryName,queryStart,queryEnd,subjectName,subjectStar,subjectEnd,E-value,score,identity)
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102 # @note data are loaded such that the query is always on the direct strand
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103 #
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104 def setFromTuple( self, tuple ):
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105 #TODO: we need to create Range instances because of __eq__() and isEmpty() tests, but WHY ???
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106 self.range_query = Range()
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107 self.range_subject = Range()
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108 if int(tuple[1]) < int(tuple[2]):
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109 self.range_query.setFromTuple( ( tuple[0], tuple[1], tuple[2] ) )
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110 self.range_subject.setFromTuple( ( tuple[3], tuple[4], tuple[5] ) )
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111 else:
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112 self.range_query.setFromTuple( ( tuple[0], tuple[2], tuple[1] ) )
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113 self.range_subject.setFromTuple( ( tuple[3], tuple[5], tuple[4] ) )
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114 self.e_value = float(tuple[6])
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115 self.score = float(tuple[7])
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116 self.identity = float(tuple[8])
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117
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118 ## Reset
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119 #
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120 def reset( self ):
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121 self.range_query.reset()
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122 self.range_subject.reset()
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123 self.e_value = 0
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124 self.score = 0
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125 self.identity = 0
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126
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127 ## Return the attributes as a formatted string
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128 #
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129 def toString(self):
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130 string = "%s" % ( self.range_query.toString() )
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131 string += "\t%s" % ( self.range_subject.toString() )
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132 string += "\t%g\t%i\t%f" % ( self.e_value, self.score, self.identity )
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133 return string
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134
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135
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136 ## Return the attributes as a GFF-formatted string
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137 #
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138 def toStringAsGff( self, source="REPET", type="match", phase=".", ID="", Parent="" ):
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139 if not self.isSubjectOnDirectStrand():
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140 self.reverse()
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141 string = "%s" % ( self.getQueryName() )
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142 string += "\t%s" % ( source )
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143 string += "\t%s" % ( type )
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144 string += "\t%s" % ( self.getQueryMin() )
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145 string += "\t%s" % ( self.getQueryMax() )
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146 string += "\t%g" % ( self.e_value )
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147 string += "\t%s" % ( self.getQueryStrand() )
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148 string += "\t%s" % ( phase )
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149 attributes = ""
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150 if ID != "":
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151 attributes += "ID=%s" % ( ID )
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152 else:
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153 attributes += "ID=%i" % ( str(time.time())[-8:-1].replace(".","") )
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154 if Parent != "":
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155 attributes += ";Parent=%s" % ( Parent )
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156 attributes += ";Target=%s %i %i" % ( self.getSubjectName(), self.getSubjectStart(), self.getSubjectEnd() )
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157 string += "\t%s" % ( attributes )
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158 return string
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159
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160
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161 ## Reverse query and subject
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162 #
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163 def reverse(self):
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164 self.range_query.reverse()
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165 self.range_subject.reverse()
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166
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167 ## Show the attributes
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168 #
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169 def show(self):
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170 print self.toString()
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171
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172 ## Write attributes into an Align file
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173 #
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174 # @param fileHandler: file handler of the file being filled
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175 #
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176 def write(self, fileHandler):
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177 fileHandler.write("%s\n" % (self.toString()))
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178
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179 ## Save attributes into an Align file
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180 #
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181 # @param file: name of the file being filled
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182 #
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183 def save(self, file):
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184 fileHandler = open( file, "a" )
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185 self.write( fileHandler )
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186 fileHandler.close()
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187
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188 ## Return the score
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189 #
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190 def getScore(self):
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diff changeset
191 return self.score
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yufei-luo
parents:
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192
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yufei-luo
parents:
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193 ## Return the identity
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yufei-luo
parents:
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194 #
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yufei-luo
parents:
diff changeset
195 def getIdentity(self):
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yufei-luo
parents:
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196 return self.identity
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yufei-luo
parents:
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197
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yufei-luo
parents:
diff changeset
198 def getEvalue(self):
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yufei-luo
parents:
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199 return self.e_value
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yufei-luo
parents:
diff changeset
200
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yufei-luo
parents:
diff changeset
201 ## Return the length on the query
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yufei-luo
parents:
diff changeset
202 #
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yufei-luo
parents:
diff changeset
203 def getLengthOnQuery(self):
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yufei-luo
parents:
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204 return self.range_query.getLength()
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yufei-luo
parents:
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205
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yufei-luo
parents:
diff changeset
206 ## Return the name of the query
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yufei-luo
parents:
diff changeset
207 #
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yufei-luo
parents:
diff changeset
208 def getQueryName( self ):
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yufei-luo
parents:
diff changeset
209 return self.range_query.seqname
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yufei-luo
parents:
diff changeset
210
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yufei-luo
parents:
diff changeset
211 ## Return the start of the query
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yufei-luo
parents:
diff changeset
212 #
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yufei-luo
parents:
diff changeset
213 def getQueryStart( self ):
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yufei-luo
parents:
diff changeset
214 return self.range_query.start
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yufei-luo
parents:
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215
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yufei-luo
parents:
diff changeset
216 ## Return the end of the query
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yufei-luo
parents:
diff changeset
217 #
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yufei-luo
parents:
diff changeset
218 def getQueryEnd( self ):
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yufei-luo
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219 return self.range_query.end
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yufei-luo
parents:
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220
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yufei-luo
parents:
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221 ## Return the min of the query
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yufei-luo
parents:
diff changeset
222 #
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yufei-luo
parents:
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223 def getQueryMin( self ):
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yufei-luo
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diff changeset
224 return self.range_query.getMin()
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yufei-luo
parents:
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225
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yufei-luo
parents:
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226 ## Return the max of the query
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yufei-luo
parents:
diff changeset
227 #
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yufei-luo
parents:
diff changeset
228 def getQueryMax( self ):
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yufei-luo
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229 return self.range_query.getMax()
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yufei-luo
parents:
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230
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yufei-luo
parents:
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231 ## Return the strand of the query
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yufei-luo
parents:
diff changeset
232 #
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yufei-luo
parents:
diff changeset
233 def getQueryStrand( self ):
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yufei-luo
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234 return self.range_query.getStrand()
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yufei-luo
parents:
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235
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yufei-luo
parents:
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236 ## Return the length on the subject
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yufei-luo
parents:
diff changeset
237 #
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yufei-luo
parents:
diff changeset
238 def getLengthOnSubject(self):
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yufei-luo
parents:
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239 return self.range_subject.getLength()
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yufei-luo
parents:
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240
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yufei-luo
parents:
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241 ## Return the name of the subject
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yufei-luo
parents:
diff changeset
242 #
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yufei-luo
parents:
diff changeset
243 def getSubjectName( self ):
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yufei-luo
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244 return self.range_subject.seqname
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yufei-luo
parents:
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245
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yufei-luo
parents:
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246 ## Return the start of the subject
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yufei-luo
parents:
diff changeset
247 #
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yufei-luo
parents:
diff changeset
248 def getSubjectStart( self ):
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yufei-luo
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249 return self.range_subject.start
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yufei-luo
parents:
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250
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yufei-luo
parents:
diff changeset
251 ## Return the end of the subject
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yufei-luo
parents:
diff changeset
252 #
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yufei-luo
parents:
diff changeset
253 def getSubjectEnd( self ):
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yufei-luo
parents:
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254 return self.range_subject.end
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yufei-luo
parents:
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255
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yufei-luo
parents:
diff changeset
256 ## Return the min of the subject
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yufei-luo
parents:
diff changeset
257 #
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yufei-luo
parents:
diff changeset
258 def getSubjectMin( self ):
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yufei-luo
parents:
diff changeset
259 return self.range_subject.getMin()
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yufei-luo
parents:
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260
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yufei-luo
parents:
diff changeset
261 ## Return the max of the subject
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yufei-luo
parents:
diff changeset
262 #
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yufei-luo
parents:
diff changeset
263 def getSubjectMax( self ):
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yufei-luo
parents:
diff changeset
264 return self.range_subject.getMax()
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yufei-luo
parents:
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265
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yufei-luo
parents:
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266 ## Return the strand of the subject
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yufei-luo
parents:
diff changeset
267 #
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yufei-luo
parents:
diff changeset
268 def getSubjectStrand( self ):
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yufei-luo
parents:
diff changeset
269 return self.range_subject.getStrand()
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yufei-luo
parents:
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270
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yufei-luo
parents:
diff changeset
271 ## Return the query as a Range instance
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yufei-luo
parents:
diff changeset
272 #
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yufei-luo
parents:
diff changeset
273 def getQueryAsRange( self ):
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yufei-luo
parents:
diff changeset
274 return self.range_query
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yufei-luo
parents:
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275
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yufei-luo
parents:
diff changeset
276 ## Return the subject as a Range instance
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yufei-luo
parents:
diff changeset
277 #
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yufei-luo
parents:
diff changeset
278 def getSubjectAsRange( self ):
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yufei-luo
parents:
diff changeset
279 return self.range_subject
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yufei-luo
parents:
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280
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yufei-luo
parents:
diff changeset
281 ## Set the name of the query
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yufei-luo
parents:
diff changeset
282 #
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yufei-luo
parents:
diff changeset
283 def setQueryName( self, name ):
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yufei-luo
parents:
diff changeset
284 self.range_query.seqname = name
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yufei-luo
parents:
diff changeset
285
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yufei-luo
parents:
diff changeset
286 ## Set the start of the query
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yufei-luo
parents:
diff changeset
287 #
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yufei-luo
parents:
diff changeset
288 def setQueryStart( self, start ):
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yufei-luo
parents:
diff changeset
289 self.range_query.start = start
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yufei-luo
parents:
diff changeset
290
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yufei-luo
parents:
diff changeset
291 ## Set the end of the query
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yufei-luo
parents:
diff changeset
292 #
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yufei-luo
parents:
diff changeset
293 def setQueryEnd( self, end ):
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yufei-luo
parents:
diff changeset
294 self.range_query.end = end
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yufei-luo
parents:
diff changeset
295
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yufei-luo
parents:
diff changeset
296 ## Set the name of the subject
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yufei-luo
parents:
diff changeset
297 #
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yufei-luo
parents:
diff changeset
298 def setSubjectName( self, name ):
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yufei-luo
parents:
diff changeset
299 self.range_subject.seqname = name
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yufei-luo
parents:
diff changeset
300
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yufei-luo
parents:
diff changeset
301 ## Set the start of the subject
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yufei-luo
parents:
diff changeset
302 #
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yufei-luo
parents:
diff changeset
303 def setSubjectStart( self, start ):
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yufei-luo
parents:
diff changeset
304 self.range_subject.start = start
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
305
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
306 ## Set the end of the subject
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yufei-luo
parents:
diff changeset
307 #
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yufei-luo
parents:
diff changeset
308 def setSubjectEnd( self, end ):
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yufei-luo
parents:
diff changeset
309 self.range_subject.end = end
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yufei-luo
parents:
diff changeset
310
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yufei-luo
parents:
diff changeset
311 ## Merge the instance with another Align instance
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yufei-luo
parents:
diff changeset
312 #
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yufei-luo
parents:
diff changeset
313 # @param o an Align instance
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yufei-luo
parents:
diff changeset
314 #
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yufei-luo
parents:
diff changeset
315 def merge(self, o):
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
316 if self.range_query.seqname != o.range_query.seqname \
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
317 or self.range_subject.seqname != o.range_subject.seqname:
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
318 return
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yufei-luo
parents:
diff changeset
319 self.range_query.merge(o.range_query)
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
320 self.range_subject.merge(o.range_subject)
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
321 self.score = max(self.score,o.score)
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
322 self.e_value = min(self.e_value,o.e_value)
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yufei-luo
parents:
diff changeset
323 self.identity = max(self.identity,o.identity)
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yufei-luo
parents:
diff changeset
324
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yufei-luo
parents:
diff changeset
325 ## Return a Map instance with the subject mapped on the query
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yufei-luo
parents:
diff changeset
326 #
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yufei-luo
parents:
diff changeset
327 def getSubjectAsMapOfQuery(self):
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yufei-luo
parents:
diff changeset
328 iMap = Map()
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yufei-luo
parents:
diff changeset
329 iMap.name = self.range_subject.seqname
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yufei-luo
parents:
diff changeset
330 iMap.seqname = self.range_query.seqname
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
331 if self.range_subject.isOnDirectStrand():
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yufei-luo
parents:
diff changeset
332 iMap.start = self.range_query.start
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yufei-luo
parents:
diff changeset
333 iMap.end = self.range_query.end
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
334 else:
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yufei-luo
parents:
diff changeset
335 iMap.start = self.range_query.end
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
336 iMap.end = self.range_query.start
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
337 return iMap
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yufei-luo
parents:
diff changeset
338
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
339 ## Return True if query is on direct strand
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yufei-luo
parents:
diff changeset
340 #
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yufei-luo
parents:
diff changeset
341 def isQueryOnDirectStrand( self ):
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yufei-luo
parents:
diff changeset
342 return self.range_query.isOnDirectStrand()
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
343
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
344 ## Return True if subject is on direct strand
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
345 #
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
346 def isSubjectOnDirectStrand( self ):
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yufei-luo
parents:
diff changeset
347 return self.range_subject.isOnDirectStrand()
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
348
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
349 ## Return True if query and subject are on the same strand, False otherwise
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
350 #
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yufei-luo
parents:
diff changeset
351 def areQrySbjOnSameStrand(self):
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yufei-luo
parents:
diff changeset
352 return self.isQueryOnDirectStrand() == self.isSubjectOnDirectStrand()
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
353
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
354 ## Return False if query and subject are on the same strand, True otherwise
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
355 #
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yufei-luo
parents:
diff changeset
356 def areQrySbjOnOppositeStrands(self):
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yufei-luo
parents:
diff changeset
357 return not self.areQrySbjOnSameStrand()
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
358
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
359 ## Set attributes from string
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yufei-luo
parents:
diff changeset
360 #
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yufei-luo
parents:
diff changeset
361 # @param string a string formatted like queryName queryStart queryEnd subjectName subjectStart subjectEnd E-value score identity
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yufei-luo
parents:
diff changeset
362 # @param sep field separator
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yufei-luo
parents:
diff changeset
363 #
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yufei-luo
parents:
diff changeset
364 def setFromString(self, string, sep="\t"):
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
365 if string[-1] == "\n":
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
366 string = string[:-1]
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yufei-luo
parents:
diff changeset
367 self.setFromTuple( string.split(sep) )
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yufei-luo
parents:
diff changeset
368
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yufei-luo
parents:
diff changeset
369 ## Return a first Map instance for the query and a second for the subject
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yufei-luo
parents:
diff changeset
370 #
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yufei-luo
parents:
diff changeset
371 def getMapsOfQueryAndSubject(self):
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yufei-luo
parents:
diff changeset
372 iMapQuery = Map( name="repet",
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
373 seqname=self.range_query.seqname,
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
374 start=self.range_query.start,
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
375 end=self.range_query.end )
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
376 iMapSubject = Map( name="repet",
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
377 seqname=self.range_subject.seqname,
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
378 start=self.range_subject.start,
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yufei-luo
parents:
diff changeset
379 end=self.range_subject.end )
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
380 return iMapQuery, iMapSubject
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yufei-luo
parents:
diff changeset
381
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
382 ## Write query coordinates as Map in a file
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yufei-luo
parents:
diff changeset
383 #
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yufei-luo
parents:
diff changeset
384 # @param fileHandler: file handler of the file being filled
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yufei-luo
parents:
diff changeset
385 #
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yufei-luo
parents:
diff changeset
386 def writeSubjectAsMapOfQuery( self, fileHandler ):
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yufei-luo
parents:
diff changeset
387 m = self.getSubjectAsMapOfQuery()
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
388 m.write( fileHandler )
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yufei-luo
parents:
diff changeset
389
769e306b7933 Change the repository level.
yufei-luo
parents:
diff changeset
390 ## Return a bin for fast database access
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yufei-luo
parents:
diff changeset
391 #
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yufei-luo
parents:
diff changeset
392 def getBin(self):
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yufei-luo
parents:
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393 return self.range_query.getBin()
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394
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395 ## Switch query and subject
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396 #
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397 def switchQuerySubject( self ):
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398 tmpRange = self.range_query
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399 self.range_query = self.range_subject
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400 self.range_subject = tmpRange
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401 if not self.isQueryOnDirectStrand():
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402 self.reverse()
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403
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404 ## Return True if the query overlaps with the query of another Align instance, False otherwise
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405 #
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406 def isQueryOverlapping( self, iAlign ):
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407 return self.getQueryAsRange().isOverlapping( iAlign.getQueryAsRange() )
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408
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409 ## Return True if the subject overlaps with the subject of another Align instance, False otherwise
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410 #
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411 def isSubjectOverlapping( self, iAlign ):
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412 return self.getSubjectAsRange().isOverlapping( iAlign.getSubjectAsRange() )
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413
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414 ## Return True if the Align instance overlaps with another Align instance, False otherwise
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415 #
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416 def isOverlapping( self, iAlign ):
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417 if self.isQueryOverlapping( iAlign ) and self.isSubjectOverlapping( iAlign ):
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418 return True
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419 else:
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420 return False
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421
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422 ## Update the score
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423 #
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424 # @note the new score is the length on the query times the percentage of identity
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425 #
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426 def updateScore( self ):
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427 newScore = self.getLengthOnQuery() * self.getIdentity() / 100.0
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428 self.score = newScore