Mercurial > repos > yufei-luo > s_mart
annotate smart_toolShed/SMART/Java/Python/FindOverlapsOptim.py @ 1:30c5431d8ce4
Uploaded
author | yufei-luo |
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date | Thu, 17 Jan 2013 11:01:51 -0500 |
parents | e0f8dcca02ed |
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rev | line source |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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1 #! /usr/bin/env python |
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2 # |
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3 # Copyright INRA-URGI 2009-2012 |
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4 # |
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5 # This software is governed by the CeCILL license under French law and |
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6 # abiding by the rules of distribution of free software. You can use, |
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7 # modify and/ or redistribute the software under the terms of the CeCILL |
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8 # license as circulated by CEA, CNRS and INRIA at the following URL |
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9 # "http://www.cecill.info". |
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10 # |
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11 # As a counterpart to the access to the source code and rights to copy, |
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12 # modify and redistribute granted by the license, users are provided only |
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13 # with a limited warranty and the software's author, the holder of the |
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14 # economic rights, and the successive licensors have only limited |
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15 # liability. |
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16 # |
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17 # In this respect, the user's attention is drawn to the risks associated |
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18 # with loading, using, modifying and/or developing or reproducing the |
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19 # software by the user in light of its specific status of free software, |
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20 # that may mean that it is complicated to manipulate, and that also |
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21 # therefore means that it is reserved for developers and experienced |
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22 # professionals having in-depth computer knowledge. Users are therefore |
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23 # encouraged to load and test the software's suitability as regards their |
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24 # requirements in conditions enabling the security of their systems and/or |
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25 # data to be ensured and, more generally, to use and operate it in the |
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26 # same conditions as regards security. |
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27 # |
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28 # The fact that you are presently reading this means that you have had |
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29 # knowledge of the CeCILL license and that you accept its terms. |
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30 # |
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31 |
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32 import os, struct, time, shutil |
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33 from optparse import OptionParser |
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34 from commons.core.parsing.ParserChooser import ParserChooser |
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35 from commons.core.writer.Gff3Writer import Gff3Writer |
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36 from SMART.Java.Python.structure.Transcript import Transcript |
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37 from SMART.Java.Python.structure.Interval import Interval |
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38 from SMART.Java.Python.ncList.NCList import NCList |
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39 from SMART.Java.Python.ncList.ConvertToNCList import ConvertToNCList |
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40 from SMART.Java.Python.ncList.NCListParser import NCListParser |
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41 from SMART.Java.Python.ncList.NCListCursor import NCListCursor |
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42 from SMART.Java.Python.ncList.NCListFilePickle import NCListFilePickle, NCListFileUnpickle |
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43 from SMART.Java.Python.ncList.NCListHandler import NCListHandler |
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44 from SMART.Java.Python.misc.Progress import Progress |
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45 from SMART.Java.Python.misc.UnlimitedProgress import UnlimitedProgress |
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46 try: |
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47 import cPickle as pickle |
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48 except: |
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49 import pickle |
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50 |
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51 REFERENCE = 0 |
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52 QUERY = 1 |
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53 TYPES = (REFERENCE, QUERY) |
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54 TYPETOSTRING = {0: "reference", 1: "query"} |
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55 |
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56 class FindOverlapsOptim(object): |
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57 |
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58 def __init__(self, verbosity = 1): |
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59 self._parsers = {} |
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60 self._sortedFileNames = {} |
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61 self._outputFileName = "outputOverlaps.gff3" |
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62 self._iWriter = None |
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63 self._inputFileNames = {REFERENCE: None, QUERY: None} |
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64 self._convertedFileNames = {REFERENCE: False, QUERY: False} |
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65 self._inputFileFormats = {REFERENCE: None, QUERY: None} |
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66 self._converted = {REFERENCE: False, QUERY: False} |
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67 self._ncListHandlers = {REFERENCE: None, QUERY: None} |
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68 self._splittedFileNames = {REFERENCE: {}, QUERY: {}} |
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69 self._nbOverlappingQueries = 0 |
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70 self._nbOverlaps = 0 |
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71 self._nbLines = {REFERENCE: 0, QUERY: 0} |
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72 self._sorted = False |
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73 self._index = False |
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74 self._verbosity = verbosity |
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75 self._ncLists = {} |
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76 self._cursors = {} |
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77 self._nbElementsPerChromosome = {} |
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78 self._tmpDirectories = {REFERENCE: False, QUERY: False} |
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79 |
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80 def close(self): |
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81 self._iWriter.close() |
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82 for fileName in (self._sortedFileNames.values()): |
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83 if os.path.exists(fileName): |
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84 os.remove(fileName) |
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85 for fileName in self._convertedFileNames.values(): |
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86 if fileName: |
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87 os.remove(fileName) |
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88 |
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89 def setRefFileName(self, fileName, format): |
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90 self.setFileName(fileName, format, REFERENCE) |
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91 |
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92 def setQueryFileName(self, fileName, format): |
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93 self.setFileName(fileName, format, QUERY) |
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94 |
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95 def setFileName(self, fileName, format, type): |
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96 self._inputFileNames[type] = fileName |
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97 self._inputFileFormats[type] = format |
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98 if format.lower() != "nclist": |
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99 self._converted[type] = True |
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100 |
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101 def setOutputFileName(self, outputFileName): |
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102 self._outputFileName = outputFileName |
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103 self._iWriter = Gff3Writer(self._outputFileName) |
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104 |
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105 def setSorted(self, sorted): |
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106 self._sorted = sorted |
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107 |
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108 def setIndex(self, index): |
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109 self._index = index |
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110 |
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111 def createNCLists(self): |
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112 startTime = time.time() |
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113 if self._verbosity > 1: |
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114 print "Building database" |
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115 self._ncLists = dict([type, {}] for type in TYPES) |
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116 self._indices = dict([type, {}] for type in TYPES) |
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117 self._cursors = dict([type, {}] for type in TYPES) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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118 for type in TYPES: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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119 self._ncListHandlers[type] = NCListHandler(self._verbosity-3) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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120 if self._converted[type]: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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121 self._convertedFileNames[type] = "%s_%d.ncl" % (os.path.splitext(self._inputFileNames[type])[0], type) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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122 ncLists = ConvertToNCList(self._verbosity-3) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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123 ncLists.setInputFileName(self._inputFileNames[type], self._inputFileFormats[type]) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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124 ncLists.setSorted(self._sorted) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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125 ncLists.setOutputFileName(self._convertedFileNames[type]) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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126 if type == REFERENCE and self._index: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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127 ncLists.setIndex(True) |
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128 ncLists.run() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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129 self._ncListHandlers[type].setFileName(self._convertedFileNames[type]) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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130 else: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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131 self._ncListHandlers[type].setFileName(self._inputFileNames[type]) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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132 self._ncListHandlers[type].loadData() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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133 self._nbLines[type] = self._ncListHandlers[type].getNbElements() |
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134 self._nbElementsPerChromosome[type] = self._ncListHandlers[type].getNbElementsPerChromosome() |
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135 self._ncLists[type] = self._ncListHandlers[type].getNCLists() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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136 for chromosome, ncList in self._ncLists[type].iteritems(): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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137 self._cursors[type][chromosome] = NCListCursor(None, ncList, 0, self._verbosity) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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138 if type == REFERENCE and self._index: |
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139 self._indices[REFERENCE][chromosome] = ncList.getIndex() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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140 endTime = time.time() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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141 if self._verbosity > 1: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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142 print "done (%.2gs)" % (endTime - startTime) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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143 |
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144 def compare(self): |
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145 nbSkips, nbMoves = 0, 0 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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146 previousChromosome = None |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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147 done = False |
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148 startTime = time.time() |
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149 progress = Progress(len(self._ncLists[QUERY].keys()), "Checking overlap", self._verbosity) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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150 #print "query:", self._ncLists[QUERY].keys() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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151 #print "reference:", self._ncLists[REFERENCE].keys() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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152 for chromosome, queryNCList in self._ncLists[QUERY].iteritems(): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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153 queryParser = self._ncListHandlers[QUERY].getParser(chromosome) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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154 queryCursor = self._cursors[QUERY][chromosome] |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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155 if chromosome != previousChromosome: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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156 skipChromosome = False |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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157 previousChromosome = chromosome |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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158 if chromosome not in self._ncLists[REFERENCE]: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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159 #print "out ", chromosome |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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160 continue |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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161 refNCList = self._ncLists[REFERENCE][chromosome] |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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162 refCursor = self._cursors[REFERENCE][chromosome] |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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163 #print "starting", chromosome |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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164 while True: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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165 queryTranscript = queryCursor.getTranscript() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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166 newRefLaddr = self.checkIndex(queryTranscript, refCursor) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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167 #print "query is", queryTranscript |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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168 if newRefLaddr != None: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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169 nbMoves += 1 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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170 refCursor.setLIndex(newRefLaddr) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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171 #print "skipping to", refCursor |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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172 done = False |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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173 refCursor, done, unmatched = self.findOverlapIter(queryTranscript, refCursor, done) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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174 #print "completed with", refCursor, done, unmatched |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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175 if refCursor.isOut(): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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176 #print "exiting 1", chromosome |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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177 break |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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178 if unmatched or not queryCursor.hasChildren(): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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179 queryCursor.moveNext() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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180 #print "moving next to", queryCursor |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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181 nbSkips += 1 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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182 else: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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183 queryCursor.moveDown() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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184 #print "moving down to", queryCursor |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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185 if queryCursor.isOut(): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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186 #print "exiting 2", chromosome |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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187 break |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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188 progress.inc() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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parents:
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189 progress.done() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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parents:
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190 endTime = time.time() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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191 self._timeSpent = endTime - startTime |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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192 if self._verbosity >= 10: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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193 print "# skips: %d" % (nbSkips) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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194 print "# moves: %d" % (nbMoves) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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195 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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196 def findOverlapIter(self, queryTranscript, cursor, done): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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197 chromosome = queryTranscript.getChromosome() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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198 if chromosome not in self._ncLists[REFERENCE]: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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199 return False, None |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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200 ncList = self._ncLists[REFERENCE][chromosome] |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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201 overlappingNames = {} |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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202 nextDone = False |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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203 firstOverlapLAddr = NCListCursor(cursor) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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204 firstOverlapLAddr.setLIndex(-1) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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205 if cursor.isOut(): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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206 return firstOverlapLAddr, False |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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207 parentCursor = NCListCursor(cursor) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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208 parentCursor.moveUp() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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209 firstParentAfter = False |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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parents:
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210 #print "query transcript 1", queryTranscript |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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211 #print "cursor 1", cursor |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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212 #print "parent 1", parentCursor |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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213 while not parentCursor.isOut(): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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214 if self.isOverlapping(queryTranscript, parentCursor) == 0: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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215 #print "overlap parent choice 0" |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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216 overlappingNames.update(self._extractID(parentCursor.getTranscript())) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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parents:
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217 if firstOverlapLAddr.isOut(): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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218 #print "overlap parent 2" |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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219 firstOverlapLAddr.copy(parentCursor) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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220 nextDone = True # new |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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221 elif self.isOverlapping(queryTranscript, parentCursor) == 1: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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222 #print "overlap parent choice 1" |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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223 firstParentAfter = NCListCursor(parentCursor) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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224 parentCursor.moveUp() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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225 #print "parent 2", parentCursor |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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226 if firstParentAfter: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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227 #print "exit parent", firstParentAfter, overlappingNames |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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228 self._writeIntervalInNewGFF3(queryTranscript, overlappingNames) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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229 return firstParentAfter, False, not overlappingNames |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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230 #This loop finds the overlaps with currentRefLAddr.# |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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231 while True: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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232 #print "ref cursor now is", cursor |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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233 parentCursor = NCListCursor(cursor) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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234 parentCursor.moveUp() |
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235 #In case: Query is on the right of the RefInterval and does not overlap. |
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236 overlap = self.isOverlapping(queryTranscript, cursor) |
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237 if overlap == -1: |
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238 cursor.moveNext() |
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239 #In case: Query overlaps with RefInterval. |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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240 elif overlap == 0: |
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241 #print "choice 2" |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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242 overlappingNames.update(self._extractID(cursor.getTranscript())) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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243 if firstOverlapLAddr.compare(parentCursor): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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244 firstOverlapLAddr.copy(cursor) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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245 nextDone = True # new |
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246 if done: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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247 cursor.moveNext() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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248 else: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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249 if not cursor.hasChildren(): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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250 cursor.moveNext() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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251 if cursor.isOut(): |
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252 #print "break 1" |
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253 break |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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254 else: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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255 cursor.moveDown() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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256 #In case: Query is on the left of the RefInterval and does not overlap. |
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257 else: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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258 #print "choice 3" |
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259 if firstOverlapLAddr.isOut() or firstOverlapLAddr.compare(parentCursor): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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260 #print "changing nfo 2" |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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261 firstOverlapLAddr.copy(cursor) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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262 nextDone = False # new |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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263 #print "break 2" |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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264 break |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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265 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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266 done = False |
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267 if cursor.isOut(): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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268 #print "break 3" |
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269 break |
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270 self._writeIntervalInNewGFF3(queryTranscript, overlappingNames) |
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271 return firstOverlapLAddr, nextDone, not overlappingNames |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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272 |
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273 def isOverlapping(self, queryTranscript, refTranscript): |
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274 if (queryTranscript.getStart() <= refTranscript.getEnd() and queryTranscript.getEnd() >= refTranscript.getStart()): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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275 return 0 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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276 if queryTranscript.getEnd() < refTranscript.getStart(): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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277 return 1 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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278 return -1 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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279 |
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280 def checkIndex(self, transcript, cursor): |
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281 if not self._index: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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282 return None |
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283 chromosome = transcript.getChromosome() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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284 nextLIndex = self._indices[REFERENCE][chromosome].getIndex(transcript) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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285 if nextLIndex == None: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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286 return None |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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287 ncList = self._ncLists[REFERENCE][chromosome] |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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288 nextGffAddress = ncList.getRefGffAddr(nextLIndex) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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289 thisGffAddress = cursor.getGffAddress() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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290 if nextGffAddress > thisGffAddress: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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291 return nextLIndex |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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292 return None |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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293 |
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294 def _writeIntervalInNewGFF3(self, transcript, names): |
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295 nbOverlaps = 0 |
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296 for cpt in names.values(): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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297 nbOverlaps += cpt |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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298 if not names: |
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299 return |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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300 transcript.setTagValue("overlapsWith", "--".join(sorted(names.keys()))) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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301 transcript.setTagValue("nbOverlaps", nbOverlaps) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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302 self._iWriter.addTranscript(transcript) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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303 self._iWriter.write() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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304 self._nbOverlappingQueries += 1 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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305 self._nbOverlaps += nbOverlaps |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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306 |
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307 def _extractID(self, transcript): |
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308 nbElements = float(transcript.getTagValue("nbElements")) if "nbElements" in transcript.getTagNames() else 1 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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309 id = transcript.getTagValue("ID") if "ID" in transcript.getTagNames() else transcript.getUniqueName() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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310 return {id: nbElements} |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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311 |
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312 def run(self): |
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313 self.createNCLists() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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314 self.compare() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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315 self.close() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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316 if self._verbosity > 0: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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317 print "# queries: %d" % (self._nbLines[QUERY]) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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318 print "# refs: %d" % (self._nbLines[REFERENCE]) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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319 print "# written: %d (%d overlaps)" % (self._nbOverlappingQueries, self._nbOverlaps) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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320 print "time: %.2gs" % (self._timeSpent) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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321 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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322 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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323 if __name__ == "__main__": |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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324 description = "Find Overlaps Optim v1.0.0: Finds overlaps with several query intervals. [Category: Data Comparison]" |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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325 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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326 parser = OptionParser(description = description) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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327 parser.add_option("-i", "--query", dest="inputQueryFileName", action="store", type="string", help="query input file [compulsory] [format: file in transcript or other format given by -f]") |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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328 parser.add_option("-f", "--queryFormat", dest="queryFormat", action="store", type="string", help="format of previous file (possibly in NCL format) [compulsory] [format: transcript or other file format]") |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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329 parser.add_option("-j", "--ref", dest="inputRefFileName", action="store", type="string", help="reference input file [compulsory] [format: file in transcript or other format given by -g]") |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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330 parser.add_option("-g", "--refFormat", dest="refFormat", action="store", type="string", help="format of previous file (possibly in NCL format) [compulsory] [format: transcript or other file format]") |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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331 parser.add_option("-o", "--output", dest="outputFileName", action="store", type="string", help="output file [compulsory] [format: output file in GFF3 format]") |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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332 parser.add_option("-d", "--index", dest="index", action="store_true", default=False, help="add an index to the reference file (faster but more memory) [format: boolean] [default: False]") |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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333 parser.add_option("-s", "--sorted", dest="sorted", action="store_true", default=False, help="input files are already sorted [format: boolean] [default: False]") |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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334 parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="Trace level [format: int] [default: 1]") |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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335 (options, args) = parser.parse_args() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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336 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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337 iFOO = FindOverlapsOptim(options.verbosity) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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338 iFOO.setRefFileName(options.inputRefFileName, options.refFormat) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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339 iFOO.setQueryFileName(options.inputQueryFileName, options.queryFormat) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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340 iFOO.setOutputFileName(options.outputFileName) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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341 iFOO.setIndex(options.index) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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342 iFOO.setSorted(options.sorted) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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343 iFOO.run() |