Mercurial > repos > yufei-luo > s_mart
annotate smart_toolShed/SMART/Java/Python/getSizes.py @ 1:30c5431d8ce4
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author | yufei-luo |
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date | Thu, 17 Jan 2013 11:01:51 -0500 |
parents | e0f8dcca02ed |
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rev | line source |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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1 #! /usr/bin/env python |
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2 # |
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3 # Copyright INRA-URGI 2009-2010 |
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4 # |
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5 # This software is governed by the CeCILL license under French law and |
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6 # abiding by the rules of distribution of free software. You can use, |
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7 # modify and/ or redistribute the software under the terms of the CeCILL |
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8 # license as circulated by CEA, CNRS and INRIA at the following URL |
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9 # "http://www.cecill.info". |
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10 # |
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11 # As a counterpart to the access to the source code and rights to copy, |
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12 # modify and redistribute granted by the license, users are provided only |
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13 # with a limited warranty and the software's author, the holder of the |
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14 # economic rights, and the successive licensors have only limited |
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15 # liability. |
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16 # |
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17 # In this respect, the user's attention is drawn to the risks associated |
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18 # with loading, using, modifying and/or developing or reproducing the |
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19 # software by the user in light of its specific status of free software, |
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20 # that may mean that it is complicated to manipulate, and that also |
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21 # therefore means that it is reserved for developers and experienced |
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22 # professionals having in-depth computer knowledge. Users are therefore |
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23 # encouraged to load and test the software's suitability as regards their |
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24 # requirements in conditions enabling the security of their systems and/or |
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25 # data to be ensured and, more generally, to use and operate it in the |
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26 # same conditions as regards security. |
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27 # |
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28 # The fact that you are presently reading this means that you have had |
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29 # knowledge of the CeCILL license and that you accept its terms. |
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30 # |
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31 import os, sys |
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32 from optparse import OptionParser |
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33 from commons.core.parsing.FastaParser import FastaParser |
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34 from commons.core.parsing.FastqParser import FastqParser |
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35 from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer |
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36 from commons.core.parsing.GffParser import GffParser |
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37 from SMART.Java.Python.misc.Progress import Progress |
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38 from SMART.Java.Python.misc.RPlotter import RPlotter |
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39 from SMART.Java.Python.misc import Utils |
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40 |
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41 from commons.core.LoggerFactory import LoggerFactory |
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42 from commons.core.utils.RepetOptionParser import RepetOptionParser |
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43 |
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44 LOG_DEPTH = "smart" |
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45 |
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46 class GetSizes(object): |
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47 |
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48 def __init__(self, inFileName = None, inFormat=None, outFileName = None, query=None,xMax=None, xMin=None, csv=False, verbosity = 0): |
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49 self.inFileName = inFileName |
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50 self.inFormat= inFormat |
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51 self.outFileName = outFileName |
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52 self.query = query |
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53 self.xMax = xMax |
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54 self.xMin = xMin |
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55 self.xLab = "Size" |
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56 self.yLab = "# reads" |
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57 self.barplot = False |
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58 self.csv = csv |
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59 self._verbosity = verbosity |
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60 self.parser = None |
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61 self._log = LoggerFactory.createLogger("%s.%s" % (LOG_DEPTH, self.__class__.__name__), self._verbosity) |
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62 |
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63 def setAttributesFromCmdLine(self): |
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64 description = "Usage: getSizes.py [options]\n\nGet Sizes v1.0.2: Get the sizes of a set of genomic coordinates. [Category: Visualization]\n" |
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65 epilog = "" |
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66 parser = RepetOptionParser(description = description, epilog = epilog) |
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67 parser.add_option("-i", "--input", dest="inputFileName", action="store", default=None, type="string", help="input file [compulsory] [format: file in transcript or sequence format given by -f]") |
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68 parser.add_option("-f", "--format", dest="format", action="store", default=None, type="string", help="format of the input [compulsory] [format: transcript or sequence file format]") |
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69 parser.add_option("-q", "--query", dest="query", action="store", default=None, type="string", help="type to mesure [default: size] [format: choice (size, intron size, exon size, 1st exon size)]") |
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70 parser.add_option("-o", "--output", dest="outputFileName", action="store", default=None, type="string", help="output file [format: output file in PNG format]") |
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71 parser.add_option("-x", "--xMax", dest="xMax", action="store", default=None, type="int", help="maximum value on the x-axis to plot [format: int]") |
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72 parser.add_option("-X", "--xMin", dest="xMin", action="store", default=None, type="int", help="minimum value on the x-axis to plot [format: int]") |
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73 parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]") |
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74 parser.add_option("-c", "--csv", dest="csv", action="store", type="string", help="write a .csv file [format: bool] [default: false]") |
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75 parser.add_option("-a", "--xLabel", dest="xLab", action="store", default="Size", type="string", help="x absis label name [format: string] [default: Size]") |
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76 parser.add_option("-b", "--yLabel", dest="yLab", action="store", default="# reads", type="string", help="y absis label name [format: string] [default: Reads]") |
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77 parser.add_option("-B", "--barplot", dest="barplot", action="store_true", default=False, help="use barplot representation [format: bool] [default: false]") |
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78 options = parser.parse_args()[0] |
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79 self._setAttributesFromOptions(options) |
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80 |
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81 def _setAttributesFromOptions(self, options): |
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82 self.setInFileName(options.inputFileName) |
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83 self.setInFormat(options.format) |
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84 self.setQuery(options.query) |
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85 self.setOutFileName(options.outputFileName) |
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86 self.setXMax(options.xMax) |
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87 self.setXMin(options.xMin) |
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88 self.setxLab(options.xLab) |
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89 self.setyLab(options.yLab) |
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90 self.setBarplot(options.barplot) |
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91 self.setVerbosity(options.verbosity) |
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92 |
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93 def setInFileName(self, inputFileName): |
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94 self.inFileName = inputFileName |
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95 |
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96 def setInFormat(self, inFormat): |
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97 self.inFormat = inFormat |
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98 |
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99 def setQuery(self, query): |
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100 self.query = query |
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101 |
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102 def setOutFileName(self, outFileName): |
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103 self.outFileName = outFileName |
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104 |
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105 def setXMax(self, xMax): |
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106 self.xMax = xMax |
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107 |
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108 def setXMin(self, xMin): |
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109 self.xMin = xMin |
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110 |
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111 def setxLab(self, xLab): |
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112 self.xLab = xLab |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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113 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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114 def setyLab(self, yLab): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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115 self.yLab = yLab |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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116 |
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117 def setBarplot(self, barplot): |
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118 self.barplot = barplot |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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119 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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parents:
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120 def setCsv(self, csv): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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121 self.csv = csv |
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122 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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123 def setVerbosity(self, verbosity): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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124 self._verbosity = verbosity |
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125 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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126 def _checkOptions(self): |
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127 if self.inFileName == None: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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128 self._logAndRaise("ERROR: Missing input file name") |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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129 if self.inFormat == "fasta": |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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130 self.parser = FastaParser(self.inFileName, self._verbosity) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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131 elif self.inFormat == "fastq": |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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132 self.parser = FastqParser(self.inFileName, self._verbosity) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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133 else: |
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134 self.parser = TranscriptContainer(self.inFileName, self.inFormat, self._verbosity) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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135 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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136 def _logAndRaise(self, errorMsg): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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137 self._log.error(errorMsg) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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138 raise Exception(errorMsg) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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139 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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140 def run(self): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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141 LoggerFactory.setLevel(self._log, self._verbosity) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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142 self._checkOptions() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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143 self._log.info("START getsizes") |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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144 self._log.debug("Input file name: %s" % self.inFileName) |
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145 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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146 nbItems = self.parser.getNbItems() |
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147 self._log.info( "%i items found" % (nbItems)) |
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148 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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149 # treat items |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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150 progress = Progress(nbItems, "Analyzing sequences of %s" % (self.inFileName), self._verbosity) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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151 sizes = {} |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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152 names = {} |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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153 minimum = 1000000000000 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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154 maximum = 0 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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155 sum = 0 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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156 number = 0 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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157 nbSubItems = 0 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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158 for item in self.parser.getIterator(): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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159 items = [] |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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160 if self.query == "exon": |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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161 items = item.getExons() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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162 elif self.query == "exon1": |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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163 if len(item.getExons()) > 1: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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164 item.sortExons() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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165 items = [item.getExons()[0]] |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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166 elif self.query == "intron": |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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parents:
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167 items = item.getIntrons() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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168 else: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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169 items = [item, ] |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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170 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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171 for thisItem in items: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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172 try: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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173 nbElements = int(float(thisItem.getTagValue("nbElements"))) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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174 if nbElements == None: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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parents:
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175 nbElements = 1 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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176 except: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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177 nbElements = 1 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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178 size = thisItem.getSize() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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179 minimum = min(minimum, size) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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180 maximum = max(maximum, size) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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181 name = thisItem.name.split()[0] |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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182 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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183 if size not in sizes: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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184 sizes[size] = nbElements |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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185 if self.csv: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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186 names[size] = [name, ] |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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187 else: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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188 sizes[size] += nbElements |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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189 if self.csv: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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|
190 names[size].append(name) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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191 sum += size |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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192 nbSubItems += nbElements |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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193 number += 1 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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194 progress.inc() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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195 progress.done() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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196 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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197 if self.outFileName != None: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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198 plotter = RPlotter(self.outFileName, self._verbosity) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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199 plotter.setFill(0) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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200 plotter.setMinimumX(self.xMin) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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201 plotter.setMaximumX(self.xMax) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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202 plotter.setXLabel(self.xLab) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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203 plotter.setYLabel(self.yLab) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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204 plotter.setBarplot(self.barplot) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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205 plotter.addLine(sizes) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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206 plotter.plot() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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|
207 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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parents:
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208 if nbSubItems == 0: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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209 self._logAndRaise("No item found") |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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|
210 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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211 if self.csv: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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|
212 csvHandle = open(self.csv, "w") |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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213 for size in range(min(sizes.keys()), max(sizes.keys())+1): |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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|
214 if size not in sizes: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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|
215 csvHandle.write("%d,0,\n" % (size)) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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|
216 else: |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
diff
changeset
|
217 csvHandle.write("%d,%d,%s\n" % (size, sizes[size], ";".join(names[size]))) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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|
218 csvHandle.close() |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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|
219 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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220 self.items = number |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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221 self.subItems = nbSubItems |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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|
222 self.nucleotides = sum |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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223 self.minAvgMedMax = Utils.getMinAvgMedMax(sizes) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
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|
224 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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|
225 print "%d items" % (number) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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|
226 print "%d sub-items" % (nbSubItems) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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|
227 print "%d nucleotides" % (sum) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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228 print "min/avg/med/max transcripts: %d/%.2f/%.1f/%d" % Utils.getMinAvgMedMax(sizes) |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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|
229 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
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230 self._log.info("END getsizes") |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
diff
changeset
|
231 |
e0f8dcca02ed
Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
diff
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|
232 |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
diff
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|
233 if __name__ == "__main__": |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
diff
changeset
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234 iGetSizes = GetSizes() |
e0f8dcca02ed
Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
diff
changeset
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235 iGetSizes.setAttributesFromCmdLine() |
e0f8dcca02ed
Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
diff
changeset
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236 iGetSizes.run() |
e0f8dcca02ed
Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
diff
changeset
|
237 |
e0f8dcca02ed
Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
yufei-luo
parents:
diff
changeset
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238 #TODO: add two more options!!!!!! |