Mercurial > repos > yufei-luo > s_mart
annotate smart_toolShed/SMART/Java/Python/structure/Sequence.py @ 1:30c5431d8ce4
Uploaded
author | yufei-luo |
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date | Thu, 17 Jan 2013 11:01:51 -0500 |
parents | e0f8dcca02ed |
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rev | line source |
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Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
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1 # |
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2 # Copyright INRA-URGI 2009-2010 |
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3 # |
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4 # This software is governed by the CeCILL license under French law and |
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5 # abiding by the rules of distribution of free software. You can use, |
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6 # modify and/ or redistribute the software under the terms of the CeCILL |
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7 # license as circulated by CEA, CNRS and INRIA at the following URL |
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8 # "http://www.cecill.info". |
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9 # |
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10 # As a counterpart to the access to the source code and rights to copy, |
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11 # modify and redistribute granted by the license, users are provided only |
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12 # with a limited warranty and the software's author, the holder of the |
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13 # economic rights, and the successive licensors have only limited |
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14 # liability. |
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15 # |
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16 # In this respect, the user's attention is drawn to the risks associated |
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17 # with loading, using, modifying and/or developing or reproducing the |
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18 # software by the user in light of its specific status of free software, |
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19 # that may mean that it is complicated to manipulate, and that also |
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20 # therefore means that it is reserved for developers and experienced |
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21 # professionals having in-depth computer knowledge. Users are therefore |
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22 # encouraged to load and test the software's suitability as regards their |
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23 # requirements in conditions enabling the security of their systems and/or |
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24 # data to be ensured and, more generally, to use and operate it in the |
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25 # same conditions as regards security. |
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26 # |
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27 # The fact that you are presently reading this means that you have had |
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28 # knowledge of the CeCILL license and that you accept its terms. |
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29 # |
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30 import sys |
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31 import re |
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32 from commons.core.seq.Bioseq import Bioseq |
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33 |
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34 reverseComplementString = { |
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35 "A": "T", |
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36 "C": "G", |
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37 "G": "C", |
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38 "T": "A", |
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39 "U": "A", |
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40 "M": "K", |
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41 "R": "Y", |
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42 "W": "W", |
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43 "S": "S", |
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44 "Y": "R", |
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45 "K": "M", |
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46 "V": "B", |
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47 "H": "D", |
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48 "D": "H", |
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49 "B": "V", |
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50 "N": "N", |
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51 "a": "t", |
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52 "c": "g", |
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53 "g": "c", |
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54 "t": "a", |
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55 "u": "a", |
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56 "m": "k", |
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57 "r": "y", |
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58 "w": "w", |
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59 "s": "s", |
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60 "y": "r", |
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61 "k": "m", |
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62 "v": "b", |
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63 "h": "d", |
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64 "d": "h", |
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65 "b": "v", |
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66 "n": "n" |
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67 } |
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68 |
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69 class Sequence(Bioseq): |
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70 """A class that codes for a sequence""" |
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71 |
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72 def __init__(self, name = "", sequence = ""): |
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73 super(Sequence, self).__init__(name, sequence) |
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74 self.name = self.header |
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75 self.quality = None |
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76 self.chunkedSequence = None |
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77 self.chunkedQuality = None |
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78 self.integerQuality = False |
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79 |
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80 def setName(self, name=""): |
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81 super(Sequence, self).setHeader(name) |
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82 |
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83 def getName(self): |
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84 return self.getHeader() |
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85 |
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86 def setSequence(self, seq=""): |
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87 super(Sequence, self).setSequence(seq) |
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88 |
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89 def setQuality(self, quality): |
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90 if quality == None: |
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91 self.quality = None |
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92 return |
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93 if " " in quality: |
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94 self.quality = quality.split() |
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95 self.integerQuality = True |
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96 else: |
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97 self.quality = list(quality) |
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98 |
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99 def getQuality(self): |
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100 if self.quality == None: |
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101 return None |
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102 if self.integerQuality: |
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103 return " ".join(self.quality) |
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104 return "".join(self.quality) |
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105 |
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106 def getSize(self): |
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107 return len(self.getSequence()) |
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108 |
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109 |
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110 def copy(self, sequence): |
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111 self.setName(sequence.getName()) |
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112 self.setSequence(sequence.getSequence()) |
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113 self.setQuality(sequence.getQuality()) |
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114 self.chunkedSequence = None |
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115 self.chunkedQuality = None |
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116 |
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117 |
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118 def chunkSequence(self): |
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119 self.chunkedSequence = [] |
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120 for i in range (0, self.getSize() / 60 + 1): |
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121 self.chunkedSequence.append(self.getSequence()[i * 60 : min(self.getSize(), (i+1) * 60)]) |
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122 if self.quality != None: |
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123 self.chunkedQuality = [] |
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124 for i in range (0, self.getSize() / 60 + 1): |
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125 self.chunkedQuality.append(self.quality[i * 60 : min(self.getSize(), (i+1) * 60)]) |
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126 |
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127 def concatenate(self, seq): |
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128 sequence = self.getSequence() |
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129 sequence += seq.getSequence() |
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130 self.setSequence(sequence) |
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131 if self.quality != None: |
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132 sep = " " if self.integerQuality else "" |
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133 self.setQuality(self.getQuality() + sep + seq.getQuality()) |
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134 self.chunkedSequence = None |
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135 self.chunkedQuality = None |
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136 |
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137 |
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138 def printFasta(self): |
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139 if self.chunkedSequence == None: |
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140 self.chunkSequence() |
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141 return ">%s\n%s\n" % (self.getHeader(), "\n".join(self.chunkedSequence)) |
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142 |
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143 |
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144 def printFastq(self): |
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145 if self.chunkedSequence == None: |
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146 self.chunkSequence() |
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147 return "@%s\n%s\n+%s\n%s\n" % (self.getHeader(), self.getSequence(), self.getHeader(), self.getQuality()) |
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148 |
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149 |
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150 def reverseComplement(self): |
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151 seq = "" |
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152 self.chunkedSequence = None |
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153 self.chunkedQuality = None |
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154 for i in range(0, self.getSize()): |
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155 char = self.getSequence()[i:i+1] |
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156 if char not in reverseComplementString: |
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157 sys.exit("Cannot understand character %s from string %s" % (char, self.getSequence())) |
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158 seq = "%s%s" % (reverseComplementString[char], seq) |
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159 self.setSequence(seq) |
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160 if self.quality != None: |
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161 self.quality = self.quality[::-1] |
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162 |
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163 |
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164 def containsAmbiguousNucleotides(self): |
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165 m = re.search("[^ACGTUacgtu]", self.getSequence()) |
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166 if m != None: |
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167 return True |
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168 return False |
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169 |
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170 |
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171 def shrinkToFirstNucleotides(self, nbNucleotides): |
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172 self.chunkedSequence = None |
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173 self.chunkedQuality = None |
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174 self.setSequence(self.getSequence()[0:nbNucleotides]) |
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175 if self.quality != None: |
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176 self.quality = self.quality[0:nbNucleotides] |
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177 |
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178 |
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179 def shrinkToLastNucleotides(self, nbNucleotides): |
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180 self.chunkedSequence = None |
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181 self.chunkedQuality = None |
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182 self.setSequence(self.getSequence()[-nbNucleotides:]) |
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183 if self.quality != None: |
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184 self.quality = self.quality[-nbNucleotides:] |