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+ − 1 #! /usr/bin/env python
+ − 2 #
+ − 3 # Copyright INRA-URGI 2009-2010
+ − 4 #
+ − 5 # This software is governed by the CeCILL license under French law and
+ − 6 # abiding by the rules of distribution of free software. You can use,
+ − 7 # modify and/ or redistribute the software under the terms of the CeCILL
+ − 8 # license as circulated by CEA, CNRS and INRIA at the following URL
+ − 9 # "http://www.cecill.info".
+ − 10 #
+ − 11 # As a counterpart to the access to the source code and rights to copy,
+ − 12 # modify and redistribute granted by the license, users are provided only
+ − 13 # with a limited warranty and the software's author, the holder of the
+ − 14 # economic rights, and the successive licensors have only limited
+ − 15 # liability.
+ − 16 #
+ − 17 # In this respect, the user's attention is drawn to the risks associated
+ − 18 # with loading, using, modifying and/or developing or reproducing the
+ − 19 # software by the user in light of its specific status of free software,
+ − 20 # that may mean that it is complicated to manipulate, and that also
+ − 21 # therefore means that it is reserved for developers and experienced
+ − 22 # professionals having in-depth computer knowledge. Users are therefore
+ − 23 # encouraged to load and test the software's suitability as regards their
+ − 24 # requirements in conditions enabling the security of their systems and/or
+ − 25 # data to be ensured and, more generally, to use and operate it in the
+ − 26 # same conditions as regards security.
+ − 27 #
+ − 28 # The fact that you are presently reading this means that you have had
+ − 29 # knowledge of the CeCILL license and that you accept its terms.
+ − 30 #
+ − 31 """Modify the content of a FASTA file"""
+ − 32 import sys
+ − 33 from optparse import OptionParser
+ − 34 from commons.core.parsing.FastaParser import FastaParser
+ − 35 from commons.core.parsing.FastqParser import FastqParser
+ − 36 from commons.core.writer.FastaWriter import FastaWriter
+ − 37 from commons.core.writer.FastqWriter import FastqWriter
+ − 38 from SMART.Java.Python.misc.Progress import Progress
+ − 39
+ − 40
+ − 41 if __name__ == "__main__":
+ − 42
+ − 43 # parse command line
+ − 44 description = "Modify Sequence List v1.0.1: Extend or shring a list of sequences. [Category: Data Modification]"
+ − 45
+ − 46 parser = OptionParser(description = description)
+ − 47 parser.add_option("-i", "--input", dest="inputFileName",action="store", type="string", help="input file [compulsory] [format: file in format given by -f]")
+ − 48 parser.add_option("-o", "--output", dest="outputFileName", action="store",default=None, type="string", help="output file [compulsory] [format: output file in format given by -f]")
+ − 49 parser.add_option("-f", "--format", dest="format",action="store",type="string", help="format of the file [compulsory] [format: sequence file format]")
+ − 50 parser.add_option("-s", "--start", dest="start", action="store", default=None,type="int",help="keep first nucleotides [format: int]")
+ − 51 parser.add_option("-e", "--end", dest="end", action="store",default=None,type="int",help="keep last nucleotides [format: int]")
+ − 52 parser.add_option("-v", "--verbosity",dest="verbosity",action="store",default=1,type="int",help="trace level [format: int]")
+ − 53 (options, args) = parser.parse_args()
+ − 54
+ − 55 if options.format == "fasta":
+ − 56 parser = FastaParser(options.inputFileName, options.verbosity)
+ − 57 writer = FastaWriter(options.outputFileName, options.verbosity)
+ − 58 elif options.format == "fastq":
+ − 59 parser = FastqParser(options.inputFileName, options.verbosity)
+ − 60 writer = FastqWriter(options.outputFileName, options.verbosity)
+ − 61 else:
+ − 62 sys.exit("Do not understand '%s' file format." % (options.format))
+ − 63
+ − 64 progress = Progress(parser.getNbSequences(), "Reading file %s" % (options.inputFileName), options.verbosity)
+ − 65 for sequence in parser.getIterator():
+ − 66 if options.start != None:
+ − 67 sequence.shrinkToFirstNucleotides(options.start)
+ − 68 if options.end != None:
+ − 69 sequence.shrinkToLastNucleotides(options.end)
+ − 70 writer.addSequence(sequence)
+ − 71 progress.inc()
+ − 72 progress.done()