annotate SMART/Java/Python/compareOverlapping.py @ 24:452e051f6562

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author m-zytnicki
date Mon, 29 Apr 2013 03:28:24 -0400
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1 #! /usr/bin/env python
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2 #
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3 # Copyright INRA-URGI 2009-2010
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4 #
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5 # This software is governed by the CeCILL license under French law and
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6 # abiding by the rules of distribution of free software. You can use,
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7 # modify and/ or redistribute the software under the terms of the CeCILL
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8 # license as circulated by CEA, CNRS and INRIA at the following URL
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9 # "http://www.cecill.info".
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10 #
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11 # As a counterpart to the access to the source code and rights to copy,
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12 # modify and redistribute granted by the license, users are provided only
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13 # with a limited warranty and the software's author, the holder of the
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14 # economic rights, and the successive licensors have only limited
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15 # liability.
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16 #
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17 # In this respect, the user's attention is drawn to the risks associated
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18 # with loading, using, modifying and/or developing or reproducing the
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19 # software by the user in light of its specific status of free software,
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20 # that may mean that it is complicated to manipulate, and that also
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21 # therefore means that it is reserved for developers and experienced
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22 # professionals having in-depth computer knowledge. Users are therefore
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23 # encouraged to load and test the software's suitability as regards their
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24 # requirements in conditions enabling the security of their systems and/or
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25 # data to be ensured and, more generally, to use and operate it in the
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26 # same conditions as regards security.
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27 #
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28 # The fact that you are presently reading this means that you have had
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29 # knowledge of the CeCILL license and that you accept its terms.
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30 #
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31 """Compare overlap of two transcript lists"""
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32 import sys
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33 import os
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34 from optparse import OptionParser
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35 from SMART.Java.Python.misc import Utils
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36 from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer
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37 from commons.core.writer.TranscriptWriter import TranscriptWriter
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38 from SMART.Java.Python.structure.TranscriptListsComparator import TranscriptListsComparator
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39 from SMART.Java.Python.misc.RPlotter import RPlotter
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40 from commons.core.writer.Gff3Writer import Gff3Writer
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41
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42 class CompareOverlapping(object):
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43
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44 def __init__(self):
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45 self._options = None
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46
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47
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48 def setAttributesFromCmdLine(self):
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49 description = "Compare Overlapping v1.0.3: Get the data which overlap with a reference set. [Category: Data Comparison]"
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50
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51 parser = OptionParser(description = description)
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52 parser.add_option("-i", "--input1", dest="inputFileName1", action="store", type="string", help="input file 1 [compulsory] [format: file in transcript format given by -f]")
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53 parser.add_option("-f", "--format1", dest="format1", action="store", type="string", help="format of file 1 [compulsory] [format: transcript file format]")
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54 parser.add_option("-j", "--input2", dest="inputFileName2", action="store", type="string", help="input file 2 [compulsory] [format: file in transcript format given by -g]")
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55 parser.add_option("-g", "--format2", dest="format2", action="store", type="string", help="format of file 2 [compulsory] [format: transcript file format]")
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56 parser.add_option("-o", "--output", dest="output", action="store", default=None, type="string", help="output file [compulsory] [format: output file in GFF3 format]")
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57 parser.add_option("-S", "--start1", dest="start1", action="store", default=None, type="int", help="only consider the n first nucleotides of the transcripts in file 1 (do not use it with -U) [format: int]")
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58 parser.add_option("-s", "--start2", dest="start2", action="store", default=None, type="int", help="only consider the n first nucleotides of the transcripts in file 2 (do not use it with -u) [format: int]")
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59 parser.add_option("-U", "--end1", dest="end1", action="store", default=None, type="int", help="only consider the n last nucleotides of the transcripts in file 1 (do not use it with -S) [format: int]")
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60 parser.add_option("-u", "--end2", dest="end2", action="store", default=None, type="int", help="only consider the n last nucleotides of the transcripts in file 2 (do not use it with -s) [format: int]")
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61 parser.add_option("-t", "--intron", dest="introns", action="store_true", default=False, help="also report introns [format: bool] [default: false]")
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62 parser.add_option("-E", "--5primeExtension1", dest="fivePrime1", action="store", default=None, type="int", help="extension towards 5' in file 1 [format: int]")
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63 parser.add_option("-e", "--5primeExtension2", dest="fivePrime2", action="store", default=None, type="int", help="extension towards 5' in file 2 [format: int]")
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64 parser.add_option("-N", "--3primeExtension1", dest="threePrime1", action="store", default=None, type="int", help="extension towards 3' in file 1 [format: int]")
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65 parser.add_option("-n", "--3primeExtension2", dest="threePrime2", action="store", default=None, type="int", help="extension towards 3' in file 2 [format: int]")
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66 parser.add_option("-c", "--colinear", dest="colinear", action="store_true", default=False, help="colinear only [format: bool] [default: false]")
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67 parser.add_option("-a", "--antisense", dest="antisense", action="store_true", default=False, help="antisense only [format: bool] [default: false]")
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68 parser.add_option("-d", "--distance", dest="distance", action="store", default=None, type="int", help="accept some distance between query and reference [format: int]")
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69 parser.add_option("-k", "--included", dest="included", action="store_true", default=False, help="keep only elements from file 1 which are included in an element of file 2 [format: bool] [default: false]")
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70 parser.add_option("-K", "--including", dest="including", action="store_true", default=False, help="keep only elements from file 2 which are included in an element of file 1 [format: bool] [default: false]")
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71 parser.add_option("-m", "--minOverlap", dest="minOverlap", action="store", default=1, type="int", help="minimum number of nucleotides overlapping to declare an overlap [format: int] [default: 1]")
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72 parser.add_option("-p", "--pcOverlap", dest="pcOverlap", action="store", default=None, type="int", help="minimum percentage of nucleotides to overlap to declare an overlap [format: int]")
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73 parser.add_option("-O", "--notOverlapping", dest="notOverlapping", action="store_true", default=False, help="also output not overlapping data [format: bool] [default: false]")
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74 parser.add_option("-x", "--exclude", dest="exclude", action="store_true", default=False, help="invert the match [format: bool] [default: false]")
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75 parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]")
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76 parser.add_option("-l", "--log", dest="log", action="store_true", default=False, help="write a log file [format: bool] [default: false]")
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77 (self._options, args) = parser.parse_args()
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78
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79
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80 def run(self):
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81 logHandle = None
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82 if self._options.log:
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83 logHandle = open(self._options.output, "w")
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84
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85 transcriptContainer1 = TranscriptContainer(self._options.inputFileName1, self._options.format1, self._options.verbosity)
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86 transcriptContainer2 = TranscriptContainer(self._options.inputFileName2, self._options.format2, self._options.verbosity)
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87 writer = TranscriptWriter(self._options.output, "gff3", self._options.verbosity)
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88
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89 transcriptListComparator = TranscriptListsComparator(logHandle, self._options.verbosity)
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90 transcriptListComparator.restrictToStart(transcriptListComparator.QUERY, self._options.start1)
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91 transcriptListComparator.restrictToStart(transcriptListComparator.REFERENCE, self._options.start2)
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92 transcriptListComparator.restrictToEnd(transcriptListComparator.QUERY, self._options.end1)
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93 transcriptListComparator.restrictToEnd(transcriptListComparator.REFERENCE, self._options.end2)
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94 transcriptListComparator.extendFivePrime(transcriptListComparator.QUERY, self._options.fivePrime1)
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95 transcriptListComparator.extendFivePrime(transcriptListComparator.REFERENCE, self._options.fivePrime2)
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96 transcriptListComparator.extendThreePrime(transcriptListComparator.QUERY, self._options.threePrime1)
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97 transcriptListComparator.extendThreePrime(transcriptListComparator.REFERENCE, self._options.threePrime2)
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98 transcriptListComparator.acceptIntrons(transcriptListComparator.QUERY, self._options.introns)
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99 transcriptListComparator.acceptIntrons(transcriptListComparator.REFERENCE, self._options.introns)
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100 transcriptListComparator.getAntisenseOnly(self._options.antisense)
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101 transcriptListComparator.getColinearOnly(self._options.colinear)
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102 transcriptListComparator.getInvert(self._options.exclude)
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103 transcriptListComparator.setMaxDistance(self._options.distance)
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104 transcriptListComparator.setMinOverlap(self._options.minOverlap)
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105 transcriptListComparator.setPcOverlap(self._options.pcOverlap)
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106 transcriptListComparator.setIncludedOnly(self._options.included)
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107 transcriptListComparator.setIncludingOnly(self._options.including)
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108 transcriptListComparator.includeNotOverlapping(self._options.notOverlapping)
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109 transcriptListComparator.computeOdds(True)
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110 transcriptListComparator.setInputTranscriptContainer(transcriptListComparator.QUERY, transcriptContainer1)
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111 transcriptListComparator.setInputTranscriptContainer(transcriptListComparator.REFERENCE, transcriptContainer2)
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112 transcriptListComparator.setOutputWriter(writer)
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113 transcriptListComparator.compareTranscriptList()
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114
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115 if self._options.log:
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116 logHandle.close()
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117
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118 if not self._options.exclude:
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119 odds = transcriptListComparator.getOdds()
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120 if self._options.verbosity > 0 and odds:
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121 print "min/avg/med/max transcripts: %d/%.2f/%.1f/%d" % Utils.getMinAvgMedMax(odds)
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122
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123 if __name__ == "__main__":
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124 icompareOverlapping = CompareOverlapping()
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125 icompareOverlapping.setAttributesFromCmdLine()
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126 icompareOverlapping.run()