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1 #! /usr/bin/env python
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2 #
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3 # Copyright INRA-URGI 2009-2010
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4 #
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5 # This software is governed by the CeCILL license under French law and
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6 # abiding by the rules of distribution of free software. You can use,
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7 # modify and/ or redistribute the software under the terms of the CeCILL
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8 # license as circulated by CEA, CNRS and INRIA at the following URL
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9 # "http://www.cecill.info".
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10 #
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11 # As a counterpart to the access to the source code and rights to copy,
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12 # modify and redistribute granted by the license, users are provided only
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13 # with a limited warranty and the software's author, the holder of the
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14 # economic rights, and the successive licensors have only limited
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15 # liability.
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16 #
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17 # In this respect, the user's attention is drawn to the risks associated
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18 # with loading, using, modifying and/or developing or reproducing the
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19 # software by the user in light of its specific status of free software,
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20 # that may mean that it is complicated to manipulate, and that also
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21 # therefore means that it is reserved for developers and experienced
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22 # professionals having in-depth computer knowledge. Users are therefore
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23 # encouraged to load and test the software's suitability as regards their
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24 # requirements in conditions enabling the security of their systems and/or
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25 # data to be ensured and, more generally, to use and operate it in the
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26 # same conditions as regards security.
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27 #
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28 # The fact that you are presently reading this means that you have had
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29 # knowledge of the CeCILL license and that you accept its terms.
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30 #
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31 import os, subprocess, glob, random
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32 from optparse import OptionParser
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33 from SMART.Java.Python.structure.Interval import Interval
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34 from SMART.Java.Python.structure.Transcript import Transcript
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35 from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer
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36 from SMART.Java.Python.misc.RPlotter import RPlotter
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37 from SMART.Java.Python.misc.Progress import Progress
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38 from commons.core.parsing.FastaParser import FastaParser
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39
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40 strands = [-1, 1]
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41 colors = {-1: "blue", 1: "red", 0: "black"}
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42 colorLine = "black"
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43
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44 def parseTargetField(field):
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45 strand = "+"
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46 splittedFieldSpace = field.split()
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47 splittedFieldPlus = field.split("+", 4)
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48 if len(splittedFieldSpace) == 3:
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49 id, start, end = splittedFieldSpace
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50 elif len(splittedFieldSpace) == 4:
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51 id, start, end, strand = splittedFieldSpace
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52 elif len(splittedFieldPlus) == 3:
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53 id, start, end = splittedFieldPlus
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54 elif len(splittedFieldPlus) == 4:
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55 id, start, end, strand = splittedFieldPlus
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56 else:
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57 raise Exception("Cannot parse Target field '%s'." % (field))
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58 return (id, int(start), int(end), strand)
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59
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60
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61 class SimpleTranscript(object):
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62 def __init__(self, transcript1, transcript2, color = None):
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63 self.start = max(0, transcript1.getStart() - transcript2.getStart())
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64 self.end = min(transcript2.getEnd() - transcript2.getStart(), transcript1.getEnd() - transcript2.getStart())
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65 self.strand = transcript1.getDirection() * transcript2.getDirection()
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66 self.exons = []
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67 for exon in transcript1.getExons():
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68 if exon.getEnd() >= transcript2.getStart() and exon.getStart() <= transcript2.getEnd():
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69 start = max(0, exon.getStart() - transcript2.getStart())
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70 end = min(transcript2.getEnd() - transcript2.getStart(), exon.getEnd() - transcript2.getStart())
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71 self.addExon(start, end, self.strand, color)
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72
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73 def addExon(self, start, end, strand, color):
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74 exon = SimpleExon(start, end, strand, color)
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75 self.exons.append(exon)
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76
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77 def getRScript(self, yOffset, height):
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78 rString = ""
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79 previousEnd = None
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80 for exon in sorted(self.exons, key=lambda exon: exon.start):
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81 if previousEnd != None:
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82 rString += "segments(%.1f, %.1f, %.1f, %.1f, col = \"%s\")\n" % (previousEnd, yOffset + height / 4.0, exon.start, yOffset + height / 4.0, colorLine)
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83 rString += exon.getRScript(yOffset, height)
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84 previousEnd = exon.end
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85 return rString
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86
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87
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88 class SimpleExon(object):
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89 def __init__(self, start, end, strand, color = None):
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90 self.start = start
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91 self.end = end
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92 self.strand = strand
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93 self.color = color
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94
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95 def getRScript(self, yOffset, height):
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96 color = self.color if self.color != None else colors[self.strand]
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97 return "rect(%.1f, %.1f, %.1f, %.1f, col=\"%s\", border = \"%s\")\n" % (self.start, yOffset, self.end, yOffset + height / 2.0, color, colorLine)
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98
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99
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100 class Plotter(object):
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101
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102 def __init__(self, seed, index, verbosity):
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103 self.seed = seed
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104 self.index = index
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105 self.verbosity = verbosity
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106 self.maxCoverage = 0
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107 self.maxOverlap = 0
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108 self.log = ""
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109 self.merge = False
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110 self.width = 1500
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111 self.heigth = 1000
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112 self.xLabel = ""
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113 self.yLabel = ""
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114 self.title = None
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115 self.absPath = os.getcwd()
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116 self.coverageDataFileName = "tmpFile_%d_%s.dat" % (seed, index)
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117 self.coverageScript = ""
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118 self.overlapScript = ""
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119 self.outputFileName = None
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120
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121 def setOutputFileName(self, fileName):
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122 self.outputFileName = fileName
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123
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124 def setTranscript(self, transcript):
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125 self.transcript = transcript
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126 self.name = transcript.getName()
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127 self.size = transcript.getEnd() - transcript.getStart() + 1
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128 if self.title == None:
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129 self.title = self.name
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130 else:
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131 self.title += " " + self.name
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132
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133 def setTitle(self, title):
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134 self.title = title + " " + self.name
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135
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136 def setPlotSize(self, width, height):
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137 self.width = width
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138 self.height = height
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139
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140 def setLabels(self, xLabel, yLabel):
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141 self.xLabel = xLabel
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142 self.yLabel = yLabel
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143
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144 def setMerge(self, merge):
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145 self.merge = merge
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146
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147 def setCoverageData(self, coverage):
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148 outputCoveragePerStrand = dict([strand, 0] for strand in strands)
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149 outputCoverage = 0
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150 dataFile = open(os.path.abspath(self.coverageDataFileName), "w")
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151 for position in range(self.size+1):
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152 sumValue = 0
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153 found = False
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154 dataFile.write("%d\t" % (position))
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155 for strand in strands:
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156 value = coverage[strand].get(position, 0)
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157 sumValue += value
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158 dataFile.write("%d\t" % (value))
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159 if value > 0:
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160 found = True
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161 outputCoveragePerStrand[strand] += 1
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162 self.maxCoverage = max(self.maxCoverage, sumValue)
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163 dataFile.write("%d\n" % (sumValue))
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164 if found:
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165 outputCoverage += 1
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166 dataFile.close()
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167 self.log += "%s (%d nt):\n - both strands: %d (%.0f%%)\n - (+) strand: %d (%.0f%%)\n - (-) strand: %d (%.0f%%)\n" % (self.name, self.size, outputCoverage, float(outputCoverage) / self.size * 100, outputCoveragePerStrand[1], float(outputCoveragePerStrand[1]) / self.size * 100, outputCoveragePerStrand[-1], float(outputCoveragePerStrand[-1]) / self.size * 100)
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168 self.coverageScript += "data = scan(\"%s\", list(pos = -666, minus = -666, plus = -666, sumValue = -666), sep=\"\t\")\n" % (os.path.abspath(self.coverageDataFileName))
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169 self.coverageScript += "lines(x = data$pos, y = data$minus, col = \"%s\")\n" % (colors[-1])
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170 self.coverageScript += "lines(x = data$pos, y = data$plus, col = \"%s\")\n" % (colors[1])
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171 self.coverageScript += "lines(x = data$pos, y = data$sumValue, col = \"%s\")\n" % (colors[0])
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172
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173 def setOverlapData(self, overlap):
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174 height = 1
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175 self.maxOverlap = (len(overlap) + 1) * height
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176 thisElement = SimpleTranscript(self.transcript, self.transcript, "black")
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177 self.overlapScript += thisElement.getRScript(0, height)
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178 for cpt, transcript in enumerate(sorted(overlap, cmp=lambda c1, c2: c1.start - c2.start if c1.start != c2.start else c1.end - c2.end)):
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179 self.overlapScript += transcript.getRScript((cpt + 1) * height, height)
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180
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181 def getFirstLine(self, suffix = None):
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182 return "png(file = \"%s_%s%s.png\", width = %d, height = %d, bg = \"white\")\n" % (self.outputFileName, self.name, "" if suffix == None or self.merge else "_%s" % (suffix), self.width, self.height)
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183
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184 def getLastLine(self):
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185 return "dev.off()\n"
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186
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187 def startR(self, fileName, script):
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188 scriptFile = open(fileName, "w")
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189 scriptFile.write(script)
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190 scriptFile.close()
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191 command = "R CMD BATCH %s" % (fileName)
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192 status = subprocess.call(command, shell=True)
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193 if status != 0:
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194 raise Exception("Problem with the execution of script file %s, status is: %s" % (fileName, status))
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195
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196 def plot(self):
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197 print "outputfileName is written in :", self.outputFileName
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198 if self.merge:
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199 fileName = "%s_%d_%s.R" % (self.outputFileName, self.seed, self.index)
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200 plotLine = "plot(x = NA, y = NA, xlab=\"%s\", ylab=\"%s\", panel.first = grid(lwd = 1.0), xlim = c(0, %d), ylim = c(0, %d), cex.axis = 2, cex.lab = 2, cex.main=2, main = \"%s\")\n" % (self.xLabel, self.yLabel, self.size, max(self.maxCoverage, self.maxOverlap), self.title)
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201 script = self.getFirstLine() + plotLine + self.overlapScript + self.coverageScript + self.getLastLine()
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202 self.startR(fileName, script)
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203 else:
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204 fileName = "%s_%d_%s_overlap.R" % (self.outputFileName, self.seed, self.index)
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205 print "overlap file is written in :", fileName
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206 plotLine = "plot(x = NA, y = NA, xlab=\"%s\", ylab=\"%s\", panel.first = grid(lwd = 1.0), xlim = c(0, %d), ylim = c(0, %d), cex.axis = 2, cex.lab = 2, cex.main=2, main = \"%s\")\n" % (self.xLabel, self.yLabel, self.size, self.maxOverlap, self.title)
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207 script = self.getFirstLine("overlap") + plotLine + self.overlapScript + self.getLastLine()
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208 self.startR(fileName, script)
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209 fileName = "%s_%d_%s_coverage.R" % (self.outputFileName, self.seed, self.index)
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210 plotLine = "plot(x = NA, y = NA, xlab=\"%s\", ylab=\"%s\", panel.first = grid(lwd = 1.0), xlim = c(0, %d), ylim = c(0, %d), cex.axis = 2, cex.lab = 2, cex.main=2, main = \"%s\")\n" % (self.xLabel, self.yLabel, self.size, self.maxCoverage, self.title)
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211 script = self.getFirstLine("coverage") + plotLine + self.coverageScript + self.getLastLine()
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212 self.startR(fileName, script)
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213
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214
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215 class PlotParser(object):
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216
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217 def __init__(self, verbosity):
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218 self.verbosity = verbosity
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219 self.parsers = [None, None]
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220 self.sequenceParser = None
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221 self.seed = random.randint(0, 10000)
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222 self.title = ""
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223 self.merge = False
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224
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225 def __del__(self):
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226 for fileName in glob.glob("tmpFile_%d*.dat" % (self.seed)):
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227 os.remove(fileName)
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228 for fileName in glob.glob("%s*.R" % (os.path.abspath(self.outputFileName))):
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229 os.remove(fileName)
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230 for fileName in glob.glob("%s*.Rout" % (os.path.abspath(self.outputFileName))):
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231 os.remove(fileName)
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232
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233 def addInput(self, inputNb, fileName, fileFormat):
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234 if fileName == None:
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235 return
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236 self.parsers[inputNb] = TranscriptContainer(fileName, fileFormat, self.verbosity)
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237 if inputNb == 0:
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238 self.parsers[1] = self.parsers[0]
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239
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240 def addSequence(self, fileName):
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241 if fileName == None:
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242 return
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243 self.sequenceParser = FastaParser(fileName, self.verbosity)
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244
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245 def setOutput(self, fileName):
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246 self.outputFileName = fileName
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247
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248 def setPlotSize(self, width, height):
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249 self.width = width
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250 self.height = height
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251
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252 def setLabels(self, xLabel, yLabel):
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253 self.xLabel = xLabel
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254 self.yLabel = yLabel
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255
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256 def setTitle(self, title):
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257 self.title = title
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258
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259 def setMerge(self, merge):
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260 self.merge = merge
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261
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262 def initializeDataFromSequences(self):
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263 self.sizes = {}
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264 self.coverage = {}
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265 self.overlap = {}
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266 for region in self.sequenceParser.getRegions():
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267 self.sizes[region] = self.sequenceParser.getSizeOfRegion(region)
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268 self.coverage[region] = {}
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269 self.overlap[region] = []
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270 for strand in strands:
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271 self.coverage[region][strand] = {}
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272 self.coverage[region][strand][1] = 0
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273 self.coverage[region][strand][self.sizes[region]] = 0
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274
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275
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276 def initializeDataFromTranscripts(self):
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277 self.coverage = dict([i, None] for i in range(self.parsers[1].getNbTranscripts()))
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278 self.overlap = dict([i, None] for i in range(self.parsers[1].getNbTranscripts()))
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279 self.sizes = dict([i, 0] for i in range(self.parsers[1].getNbTranscripts()))
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280 self.parsers[0].findData()
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281 progress = Progress(self.parsers[1].getNbTranscripts(), "Reading regions", self.verbosity)
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282 for cpt, transcript in enumerate(self.parsers[1].getIterator()):
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283 self.coverage[cpt] = {}
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284 self.overlap[cpt] = []
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285 for strand in strands:
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286 self.coverage[cpt][strand] = {}
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287 self.coverage[cpt][strand][0] = 0
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288 self.coverage[cpt][strand][transcript.getEnd() - transcript.getStart()] = 0
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289 for exon in transcript.getExons():
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290 self.sizes[cpt] += exon.getSize()
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291 progress.inc()
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292 progress.done()
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293
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294 def initialize(self):
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295 if self.sequenceParser == None:
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296 self.initializeDataFromTranscripts()
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297 else:
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298 self.initializeDataFromSequences()
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299
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300 def computeCoverage(self, transcript1, transcript2, id):
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301 strand = transcript1.getDirection() * transcript2.getDirection()
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302 for exon1 in transcript1.getExons():
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303 for exon2 in transcript2.getExons():
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304 if exon1.overlapWith(exon2):
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305 for position in range(max(exon1.getStart(), exon2.getStart()), min(exon1.getEnd(), exon2.getEnd()) + 1):
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306 relativePosition = position - transcript2.getStart() + 1
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307 self.coverage[id][strand][relativePosition] = self.coverage[id][strand].get(relativePosition, 0) + 1
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308
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309 def computeOverlap(self, transcript1, transcript2, id):
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310 simpleTranscript = SimpleTranscript(transcript1, transcript2)
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311 self.overlap[id].append(simpleTranscript)
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312
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313 def compute2TranscriptFiles(self):
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314 progress = Progress(self.parsers[1].getNbTranscripts(), "Comparing regions", self.verbosity)
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315 for cpt2, transcript2 in enumerate(self.parsers[1].getIterator()):
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316 for transcript1 in self.parsers[0].getIterator():
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317 if transcript1.overlapWithExon(transcript2):
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318 self.computeCoverage(transcript1, transcript2, cpt2)
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319 self.computeOverlap(transcript1, transcript2, cpt2)
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320 progress.inc()
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321 progress.done()
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322
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323 def extractReferenceQuery(self, inputTranscript):
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324 if "Target" not in inputTranscript.getTagNames():
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325 raise Exception("Cannot extract Target field in line '%s'." % (inputTranscript))
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326 id, start, end, strand = parseTargetField(inputTranscript.getTagValue("Target"))
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327 if id not in self.sizes:
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328 raise Exception("Target id '%s' of transcript '%s' does not correspond to anything in FASTA file." % (id, inputTranscript))
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329 referenceTranscript = Transcript()
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330 referenceTranscript.setChromosome(id)
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331 referenceTranscript.setName(id)
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332 referenceTranscript.setDirection("+")
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333 referenceTranscript.setEnd(self.sizes[id])
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334 referenceTranscript.setStart(1)
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335 queryTranscript = Transcript()
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336 queryTranscript.setChromosome(id)
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337 queryTranscript.setName(id)
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338 queryTranscript.setStart(start)
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339 queryTranscript.setEnd(end)
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340 queryTranscript.setDirection(strand)
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341 if inputTranscript.getNbExons() > 1:
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342 factor = float(end - start) / (inputTranscript.getEnd() - inputTranscript.getStart())
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343 for exon in inputTranscript.getExons():
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344 newExon = Interval()
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345 newExon.setChromosome(id)
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346 newExon.setDirection(strand)
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347 if "Target" in inputTranscript.getTagNames():
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348 id, start, end, strand = parseTargetField(exon.getTagValue("Target"))
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349 newExon.setStart(start)
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350 newExon.setEnd(end)
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351 else:
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352 newExon.setStart(int(round((exon.getStart() - inputTranscript.getStart()) * factor)) + start)
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353 newExon.setEnd( int(round((exon.getEnd() - inputTranscript.getStart()) * factor)) + start)
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354 queryTranscript.addExon(newExon)
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355 return (referenceTranscript, queryTranscript)
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356
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357 def compute1TranscriptFiles(self):
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358 progress = Progress(self.parsers[1].getNbTranscripts(), "Comparing regions", self.verbosity)
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359 for transcript in self.parsers[1].getIterator():
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360 referenceTranscript, queryTranscript = self.extractReferenceQuery(transcript)
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361 self.computeCoverage(queryTranscript, referenceTranscript, referenceTranscript.getName())
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362 self.computeOverlap(queryTranscript, referenceTranscript, referenceTranscript.getName())
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363 progress.inc()
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364 progress.done()
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365
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366 def compute(self):
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367 if self.sequenceParser == None:
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368 self.compute2TranscriptFiles()
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369 else:
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370 self.compute1TranscriptFiles()
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371
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372 def plotTranscript(self, index, transcript):
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373 plotter = Plotter(self.seed, index, self.verbosity)
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374 plotter.setOutputFileName(self.outputFileName)
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375 plotter.setTranscript(transcript)
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376 plotter.setTitle(self.title)
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377 plotter.setLabels(self.xLabel, self.yLabel)
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378 plotter.setPlotSize(self.width, self.height)
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379 plotter.setCoverageData(self.coverage[index])
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380 plotter.setOverlapData(self.overlap[index])
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381 plotter.setMerge(self.merge)
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382 plotter.plot()
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383 output = plotter.log
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384 return output
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385
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386 def plot1TranscriptFile(self):
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387 self.outputCoverage = {}
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388 self.outputCoveragePerStrand = {}
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389 output = ""
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390 progress = Progress(len(self.sequenceParser.getRegions()), "Plotting regions", self.verbosity)
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391 for cpt2, region in enumerate(self.sequenceParser.getRegions()):
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392 transcript = Transcript()
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393 transcript.setName(region)
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394 transcript.setDirection("+")
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395 transcript.setEnd(self.sizes[region])
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396 transcript.setStart(1)
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397 output += self.plotTranscript(region, transcript)
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398 progress.inc()
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399 progress.done()
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400 if self.verbosity > 0:
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401 print output
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402
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403 def plot2TranscriptFiles(self):
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404 self.outputCoverage = [0] * self.parsers[1].getNbTranscripts()
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405 self.outputCoveragePerStrand = [None] * self.parsers[1].getNbTranscripts()
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406 for cpt in range(self.parsers[1].getNbTranscripts()):
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407 self.outputCoveragePerStrand[cpt] = dict([strand, 0] for strand in strands)
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408 progress = Progress(self.parsers[1].getNbTranscripts(), "Plotting regions", self.verbosity)
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|
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409 output = ""
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|
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410 for cpt2, transcript2 in enumerate(self.parsers[1].getIterator()):
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411 output += self.plotTranscript(cpt2, transcript2)
|
|
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412 progress.inc()
|
|
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413 progress.done()
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|
|
414 if self.verbosity > 0:
|
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415 print output
|
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416
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417 def plot(self):
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418 if self.sequenceParser == None:
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419 self.plot2TranscriptFiles()
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|
420 else:
|
|
|
421 self.plot1TranscriptFile()
|
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422
|
|
|
423 def start(self):
|
|
|
424 self.initialize()
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|
|
425 self.compute()
|
|
|
426 self.plot()
|
|
|
427
|
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|
428
|
|
|
429 if __name__ == "__main__":
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|
|
430
|
|
|
431 # parse command line
|
|
|
432 description = "Plot Coverage v1.0.1: Plot the coverage of the first data with respect to the second one. [Category: Visualization]"
|
|
|
433
|
|
|
434 parser = OptionParser(description = description)
|
|
|
435 parser.add_option("-i", "--input1", dest="inputFileName1", action="store", type="string", help="input file 1 [compulsory] [format: file in transcript format given by -f]")
|
|
|
436 parser.add_option("-f", "--inputFormat1", dest="inputFormat1", action="store", type="string", help="format of input file 1 [compulsory] [format: transcript file format]")
|
|
|
437 parser.add_option("-j", "--input2", dest="inputFileName2", action="store", type="string", help="input file 2 [compulsory] [format: file in transcript format given by -g]")
|
|
|
438 parser.add_option("-g", "--inputFormat2", dest="inputFormat2", action="store", type="string", help="format of input file 2 [compulsory] [format: transcript file format]")
|
|
|
439 parser.add_option("-q", "--sequence", dest="inputSequence", action="store", default=None, type="string", help="input sequence file [format: file in FASTA format] [default: None]")
|
|
|
440 parser.add_option("-o", "--output", dest="outputFileName", action="store", type="string", help="output file [compulsory] [format: output file in PNG format]")
|
|
|
441 parser.add_option("-w", "--width", dest="width", action="store", default=1500, type="int", help="width of the plots (in px) [format: int] [default: 1500]")
|
|
|
442 parser.add_option("-e", "--height", dest="height", action="store", default=1000, type="int", help="height of the plots (in px) [format: int] [default: 1000]")
|
|
|
443 parser.add_option("-t", "--title", dest="title", action="store", default="", type="string", help="title of the plots [format: string]")
|
|
|
444 parser.add_option("-x", "--xlab", dest="xLabel", action="store", default="", type="string", help="label on the x-axis [format: string]")
|
|
|
445 parser.add_option("-y", "--ylab", dest="yLabel", action="store", default="", type="string", help="label on the y-axis [format: string]")
|
|
|
446 parser.add_option("-p", "--plusColor", dest="plusColor", action="store", default="red", type="string", help="color for the elements on the plus strand [format: string] [default: red]")
|
|
|
447 parser.add_option("-m", "--minusColor", dest="minusColor", action="store", default="blue", type="string", help="color for the elements on the minus strand [format: string] [default: blue]")
|
|
|
448 parser.add_option("-s", "--sumColor", dest="sumColor", action="store", default="black", type="string", help="color for 2 strands coverage line [format: string] [default: black]")
|
|
|
449 parser.add_option("-l", "--lineColor", dest="lineColor", action="store", default="black", type="string", help="color for the lines [format: string] [default: black]")
|
|
|
450 parser.add_option("-1", "--merge", dest="merge", action="store_true", default=False, help="merge the 2 plots in 1 [format: boolean] [default: false]")
|
|
|
451 parser.add_option("-D", "--directory", dest="working_Dir", action="store", default=os.getcwd(), type="string", help="the directory to store the results [format: directory]")
|
|
|
452 parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]")
|
|
|
453 (options, args) = parser.parse_args()
|
|
|
454
|
|
|
455 colors[1] = options.plusColor
|
|
|
456 colors[-1] = options.minusColor
|
|
|
457 colors[0] = options.sumColor
|
|
|
458 colorLine = options.lineColor
|
|
|
459
|
|
|
460 pp = PlotParser(options.verbosity)
|
|
|
461 pp.addInput(0, options.inputFileName1, options.inputFormat1)
|
|
|
462 pp.addInput(1, options.inputFileName2, options.inputFormat2)
|
|
|
463 pp.addSequence(options.inputSequence)
|
|
|
464 if options.working_Dir[-1] != '/':
|
|
|
465 path = options.working_Dir + '/'
|
|
|
466 pp.setOutput(path + options.outputFileName)
|
|
|
467 pp.setPlotSize(options.width, options.height)
|
|
|
468 pp.setLabels(options.xLabel, options.yLabel)
|
|
|
469 pp.setTitle(options.title)
|
|
|
470 pp.setMerge(options.merge)
|
|
|
471 pp.start()
|
|
|
472
|
|
|
473
|
