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1 #! /usr/bin/env python
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2 #
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3 # Copyright INRA-URGI 2009-2010
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4 #
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5 # This software is governed by the CeCILL license under French law and
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6 # abiding by the rules of distribution of free software. You can use,
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7 # modify and/ or redistribute the software under the terms of the CeCILL
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8 # license as circulated by CEA, CNRS and INRIA at the following URL
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9 # "http://www.cecill.info".
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10 #
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11 # As a counterpart to the access to the source code and rights to copy,
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12 # modify and redistribute granted by the license, users are provided only
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13 # with a limited warranty and the software's author, the holder of the
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14 # economic rights, and the successive licensors have only limited
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15 # liability.
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16 #
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17 # In this respect, the user's attention is drawn to the risks associated
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18 # with loading, using, modifying and/or developing or reproducing the
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19 # software by the user in light of its specific status of free software,
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20 # that may mean that it is complicated to manipulate, and that also
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21 # therefore means that it is reserved for developers and experienced
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22 # professionals having in-depth computer knowledge. Users are therefore
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23 # encouraged to load and test the software's suitability as regards their
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24 # requirements in conditions enabling the security of their systems and/or
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25 # data to be ensured and, more generally, to use and operate it in the
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26 # same conditions as regards security.
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27 #
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28 # The fact that you are presently reading this means that you have had
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29 # knowledge of the CeCILL license and that you accept its terms.
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30 #
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31 """Get the repartition of some elements (# exons per transcripts, # of repetitions of a mapping or # of transcripts in a cluster)"""
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32
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33 from optparse import OptionParser
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34 from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer
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35 from SMART.Java.Python.misc.RPlotter import RPlotter
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36 from SMART.Java.Python.misc.Progress import Progress
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37 from SMART.Java.Python.misc import Utils
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38 from math import *
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39
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40 if __name__ == "__main__":
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41
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42 # parse command line
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43 description = "Get Nb v1.0.1: Get the distribution of exons per transcripts, or mapping per read, or transcript per cluster. [Category: Visualization]"
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44
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45 parser = OptionParser(description = description)
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46 parser.add_option("-i", "--input", dest="inputFileName", action="store", type="string", help="input file [compulsory] [format: file in transcript format given by -f]")
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47 parser.add_option("-f", "--format", dest="format", action="store", type="string", help="format of the input file [compulsory] [format: transcript file format]")
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48 parser.add_option("-o", "--output", dest="outputFileName", action="store", type="string", help="output file [compulsory] [format: output file in png format]")
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49 parser.add_option("-q", "--query", dest="query", action="store", type="string", help="query [compulsory] (# exons, # transcripts) [format: choice (exon, transcript, cluster)]")
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50 parser.add_option("-b", "--barplot", dest="barplot", action="store_true", default=False, help="use barplot representation [format: bool] [default: false]")
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51 parser.add_option("-x", "--xMax", dest="xMax", action="store", default=None, type="int", help="maximum value on the x-axis to plot [format: int]")
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52 parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [default: 1] [format: int]")
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53 parser.add_option("-l", "--log", dest="log", action="store_true", default=False, help="write a log file [format: bool] [default: false]")
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54 (options, args) = parser.parse_args()
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55
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56 if options.query != "exon" and options.query != "transcript" and options.query != "cluster":
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57 raise Exception("Do not understand query %s" % (options.query))
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58
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59 exonDistribution = {}
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60 transcriptDistribution = {}
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61 clusterDistribution = {}
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62
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63 transcriptContainer = TranscriptContainer(options.inputFileName, options.format, options.verbosity)
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64
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65 progress = Progress(transcriptContainer.getNbTranscripts(), "Parsing %s" % (options.inputFileName), options.verbosity)
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66 # count the number of reads
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67 for element in transcriptContainer.getIterator():
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68 if options.query == "exon":
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69 nbExons = element.getNbExons()
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70 exonDistribution[nbExons] = exonDistribution.get(nbExons, 0) + 1
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71 elif options.query == "transcript":
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72 name = element.getName()
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73 transcriptDistribution[name] = transcriptDistribution.get(name, 0) + 1
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74 elif options.query == "cluster":
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75 nbElements = 1 if "nbElements" not in element.getTagNames() else element.getTagValue("nbElements")
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76 clusterDistribution[nbElements] = clusterDistribution.get(nbElements, 0) + 1
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77 progress.inc()
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78 progress.done()
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79
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80 if options.query == "exon":
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81 distribution = exonDistribution
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82 elif options.query == "transcript":
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83 distribution = {}
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84 for name in transcriptDistribution:
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85 distribution[transcriptDistribution[name]] = distribution.get(transcriptDistribution[name], 0) + 1
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86 elif options.query == "cluster":
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87 distribution = clusterDistribution
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88
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89 outputFileName = options.outputFileName
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90 plotter = RPlotter(outputFileName, options.verbosity)
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91 plotter.setImageSize(1000, 300)
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92 plotter.setFill(0)
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93 plotter.setMaximumX(options.xMax)
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94 plotter.setBarplot(options.barplot)
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95 plotter.addLine(distribution)
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96 plotter.plot()
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97
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98 print "min/avg/med/max: %d/%.2f/%.1f/%d" % (Utils.getMinAvgMedMax(distribution))
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99
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