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1 #! /usr/bin/env python
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2 #
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3 # Copyright INRA-URGI 2009-2010
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4 #
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5 # This software is governed by the CeCILL license under French law and
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6 # abiding by the rules of distribution of free software. You can use,
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7 # modify and/ or redistribute the software under the terms of the CeCILL
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8 # license as circulated by CEA, CNRS and INRIA at the following URL
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9 # "http://www.cecill.info".
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10 #
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11 # As a counterpart to the access to the source code and rights to copy,
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12 # modify and redistribute granted by the license, users are provided only
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13 # with a limited warranty and the software's author, the holder of the
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14 # economic rights, and the successive licensors have only limited
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15 # liability.
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16 #
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17 # In this respect, the user's attention is drawn to the risks associated
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18 # with loading, using, modifying and/or developing or reproducing the
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19 # software by the user in light of its specific status of free software,
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20 # that may mean that it is complicated to manipulate, and that also
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21 # therefore means that it is reserved for developers and experienced
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22 # professionals having in-depth computer knowledge. Users are therefore
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23 # encouraged to load and test the software's suitability as regards their
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24 # requirements in conditions enabling the security of their systems and/or
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25 # data to be ensured and, more generally, to use and operate it in the
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26 # same conditions as regards security.
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27 #
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28 # The fact that you are presently reading this means that you have had
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29 # knowledge of the CeCILL license and that you accept its terms.
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30 #
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31 """Restrict a transcript list with some parameters (regions)"""
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32
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33 from optparse import OptionParser
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34 from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer
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35 from commons.core.writer.TranscriptWriter import TranscriptWriter
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36 from SMART.Java.Python.misc.Progress import Progress
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37
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38 STRAND2DIRECTION = {"+": 1, "-": -1, None: None}
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39
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40 if __name__ == "__main__":
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41
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42 # parse command line
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43 description = "Restrict Transcript List v1.0.2: Keep the coordinates which are located in a given position. [Category: Data Selection]"
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44
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45 parser = OptionParser(description = description)
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46 parser.add_option("-i", "--input", dest="inputFileName", action="store", type="string", help="input file [compulsory] [format: file in transcript format given by -f]")
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47 parser.add_option("-f", "--format", dest="format", action="store", type="string", help="format [compulsory] [format: transcript file format]")
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48 parser.add_option("-c", "--chromosome", dest="chromosome", action="store", default=None, type="string", help="chromosome [format: string]")
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49 parser.add_option("-s", "--start", dest="start", action="store", default=None, type="int", help="start [format: int]")
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50 parser.add_option("-e", "--end", dest="end", action="store", default=None, type="int", help="end [format: int]")
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51 parser.add_option("-t", "--strand", dest="strand", action="store", default=None, type="string", help="strand (+ or -) [format: string]")
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52 parser.add_option("-o", "--output", dest="outputFileName", action="store", type="string", help="output file [format: output file in GFF3 format]")
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53 parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]")
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54 (options, args) = parser.parse_args()
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55
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56 parser = TranscriptContainer(options.inputFileName, options.format, options.verbosity)
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57 writer = TranscriptWriter(options.outputFileName, options.format, options.verbosity)
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58
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59 direction = STRAND2DIRECTION[options.strand]
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60
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61 nbTranscripts = parser.getNbTranscripts()
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62 progress = Progress(nbTranscripts, "Parsing file %s" % (options.inputFileName), options.verbosity)
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63
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64 nbTotal = 0
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65 nbKept = 0
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66 for transcript in parser.getIterator():
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67 progress.inc()
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68 nbTotal += 1
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69 if options.chromosome != None and options.chromosome != transcript.getChromosome():
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70 continue
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71 if options.start != None and options.start > transcript.getEnd():
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72 continue
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73 if options.end != None and options.end < transcript.getStart():
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74 continue
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75 if options.end != None and options.end < transcript.getStart():
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76 continue
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77 if direction != None and direction != transcript.getDirection():
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78 continue
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79 nbKept += 1
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80 writer.addTranscript(transcript)
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81 progress.done()
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82
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83 writer.write()
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84
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85 print "%d out of %d are kept (%f%%)" % (nbKept, nbTotal, (float(nbKept) / nbTotal * 100))
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