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1 #! /usr/bin/env python
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2 #
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3 # Copyright INRA-URGI 2009-2010
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4 #
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5 # This software is governed by the CeCILL license under French law and
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6 # abiding by the rules of distribution of free software. You can use,
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7 # modify and/ or redistribute the software under the terms of the CeCILL
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8 # license as circulated by CEA, CNRS and INRIA at the following URL
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9 # "http://www.cecill.info".
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10 #
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11 # As a counterpart to the access to the source code and rights to copy,
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12 # modify and redistribute granted by the license, users are provided only
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13 # with a limited warranty and the software's author, the holder of the
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14 # economic rights, and the successive licensors have only limited
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15 # liability.
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16 #
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17 # In this respect, the user's attention is drawn to the risks associated
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18 # with loading, using, modifying and/or developing or reproducing the
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19 # software by the user in light of its specific status of free software,
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20 # that may mean that it is complicated to manipulate, and that also
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21 # therefore means that it is reserved for developers and experienced
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22 # professionals having in-depth computer knowledge. Users are therefore
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23 # encouraged to load and test the software's suitability as regards their
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24 # requirements in conditions enabling the security of their systems and/or
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25 # data to be ensured and, more generally, to use and operate it in the
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26 # same conditions as regards security.
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27 #
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28 # The fact that you are presently reading this means that you have had
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29 # knowledge of the CeCILL license and that you accept its terms.
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30 #
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31 from optparse import OptionParser
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32 from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer
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33 from commons.core.parsing.WigParser import WigParser
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34 from commons.core.writer.Gff3Writer import Gff3Writer
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35 from SMART.Java.Python.misc.Progress import Progress
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36
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37
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38 if __name__ == "__main__":
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39
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40 # parse command line
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41 description = "Get WIG Data v1.0.1: Compute the average data for some genomic coordinates using WIG files (thus covering a large proportion of the genome) and update a tag. [Category: WIG Tools]"
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42
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43 parser = OptionParser(description = description)
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44 parser.add_option("-i", "--input", dest="inputFileName", action="store", type="string", help="input file [compulsory] [format: file in transcript format given by -f]")
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45 parser.add_option("-f", "--inputFormat", dest="inputFormat", action="store", type="string", help="format of the input file [compulsory] [format: transcript file format]")
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46 parser.add_option("-w", "--wig", dest="wig", action="store", type="string", help="wig file name [compulsory] [format: file in WIG format]")
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47 parser.add_option("-t", "--tag", dest="tag", action="store", type="string", help="choose a tag name to write the wig information to output file [compulsory] [format: file in WIG format]")
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48 parser.add_option("-o", "--output", dest="outputFileName", action="store", type="string", help="output file [compulsory] [format: output file in GFF3 format]")
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49 parser.add_option("-s", "--strands", dest="strands", action="store_true", default=False, help="consider both strands separately [format: boolean] [default: False]")
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50 parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]")
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51 (options, args) = parser.parse_args()
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52
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53 # create parsers and writers
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54 transcriptParser = TranscriptContainer(options.inputFileName, options.inputFormat, options.verbosity)
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55 wigParser = WigParser(options.wig)
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56 writer = Gff3Writer(options.outputFileName, options.verbosity)
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57 wigParser.setStrands(options.strands)
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58
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59 progress = Progress(transcriptParser.getNbTranscripts(), "Parsing %s" % (options.inputFileName), options.verbosity)
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60 for transcript in transcriptParser.getIterator():
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61 values = transcript.extractWigData(wigParser)
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62 if options.strands:
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63 values = values[transcript.getDirection()]
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64 transcript.setTagValue(options.tag, str(float(sum(values)) / len(values)))
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65 writer.addTranscript(transcript)
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66 progress.inc()
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67 progress.done()
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