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1 #
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2 # Copyright INRA-URGI 2009-2011
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3 #
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4 # This software is governed by the CeCILL license under French law and
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5 # abiding by the rules of distribution of free software. You can use,
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6 # modify and/ or redistribute the software under the terms of the CeCILL
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7 # license as circulated by CEA, CNRS and INRIA at the following URL
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8 # "http://www.cecill.info".
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9 #
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10 # As a counterpart to the access to the source code and rights to copy,
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11 # modify and redistribute granted by the license, users are provided only
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12 # with a limited warranty and the software's author, the holder of the
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13 # economic rights, and the successive licensors have only limited
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14 # liability.
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15 #
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16 # In this respect, the user's attention is drawn to the risks associated
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17 # with loading, using, modifying and/or developing or reproducing the
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18 # software by the user in light of its specific status of free software,
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19 # that may mean that it is complicated to manipulate, and that also
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20 # therefore means that it is reserved for developers and experienced
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21 # professionals having in-depth computer knowledge. Users are therefore
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22 # encouraged to load and test the software's suitability as regards their
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23 # requirements in conditions enabling the security of their systems and/or
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24 # data to be ensured and, more generally, to use and operate it in the
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25 # same conditions as regards security.
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26 #
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27 # The fact that you are presently reading this means that you have had
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28 # knowledge of the CeCILL license and that you accept its terms.
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29 #
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30 import re
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31 import sys
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32 from commons.core.parsing.MapperParser import MapperParser
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33 from SMART.Java.Python.structure.Mapping import Mapping
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34 from SMART.Java.Python.structure.SubMapping import SubMapping
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35 from SMART.Java.Python.structure.Interval import Interval
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36
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37 class BowtieParser(MapperParser):
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38 """A class that parses BowTie format"""
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39
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40 def __init__(self, fileName, verbosity = 0):
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41 super(BowtieParser, self).__init__(fileName, verbosity)
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42
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43
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44 def __del__(self):
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45 super(BowtieParser, self).__del__()
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46
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47
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48 def getFileFormats():
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49 return ["bowtie"]
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50 getFileFormats = staticmethod(getFileFormats)
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51
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52
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53 def skipFirstLines(self):
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54 pass
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55
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56
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57 def parseLine(self, line):
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58 line = line.strip()
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59 fields = line.split("\t")
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60 if len(fields) not in (7, 8):
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61 raise Exception("Line %d '%s' does not look like a BowTie line (number of fields is %d instead of 7 or 8)" % (self.currentLineNb, line, len(fields)))
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62 name = fields[0]
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63 direction = 1 if fields[1] == "+" else -1
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64 chromosome = fields[2]
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65 genomeStart = int(fields[3]) + 1
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66 sequence = fields[4]
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67 quality = fields[5]
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68 number = int(fields[6])
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69 nbMismatches = 0
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70 if len(fields) == 8:
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71 tags = fields[7]
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72 nbMismatches = len(tags.split(","))
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73
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74 mapping = Mapping()
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75 queryInterval = Interval()
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76 queryInterval.setName(name)
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77 queryInterval.setStart(1)
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78 queryInterval.setEnd(len(sequence) + 1)
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79 targetInterval = Interval()
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80 targetInterval.setChromosome(chromosome)
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81 targetInterval.setStart(genomeStart)
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82 targetInterval.setEnd(genomeStart + len(sequence) - 1)
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83 subMapping = SubMapping()
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84 subMapping.setQueryInterval(queryInterval)
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85 subMapping.setTargetInterval(targetInterval)
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86 mapping.addSubMapping(subMapping)
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87 mapping.setSize(len(sequence))
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88 mapping.setNbMismatches(nbMismatches)
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89 mapping.setDirection(direction)
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90 return mapping
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91
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