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1 #
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2 # Copyright INRA-URGI 2009-2010
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3 #
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4 # This software is governed by the CeCILL license under French law and
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5 # abiding by the rules of distribution of free software. You can use,
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6 # modify and/ or redistribute the software under the terms of the CeCILL
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7 # license as circulated by CEA, CNRS and INRIA at the following URL
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8 # "http://www.cecill.info".
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9 #
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10 # As a counterpart to the access to the source code and rights to copy,
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11 # modify and redistribute granted by the license, users are provided only
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12 # with a limited warranty and the software's author, the holder of the
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13 # economic rights, and the successive licensors have only limited
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14 # liability.
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15 #
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16 # In this respect, the user's attention is drawn to the risks associated
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17 # with loading, using, modifying and/or developing or reproducing the
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18 # software by the user in light of its specific status of free software,
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19 # that may mean that it is complicated to manipulate, and that also
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20 # therefore means that it is reserved for developers and experienced
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21 # professionals having in-depth computer knowledge. Users are therefore
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22 # encouraged to load and test the software's suitability as regards their
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23 # requirements in conditions enabling the security of their systems and/or
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24 # data to be ensured and, more generally, to use and operate it in the
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25 # same conditions as regards security.
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26 #
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27 # The fact that you are presently reading this means that you have had
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28 # knowledge of the CeCILL license and that you accept its terms.
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29 #
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30 import sys
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31 from commons.core.parsing.SequenceListParser import SequenceListParser
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32 from SMART.Java.Python.structure.Sequence import Sequence
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33
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34 class FastqParser(SequenceListParser):
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35 """A class that reads a list of sequences in FASTQ format"""
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36
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37 def __init__(self, fileName, verbosity = 0):
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38 super(FastqParser, self).__init__(fileName, verbosity)
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39
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40
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41 def getFileFormats():
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42 return ["fastq", "mfq"]
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43 getFileFormats = staticmethod(getFileFormats)
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44
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45
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46 def getInfos(self):
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47 """
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48 Get some generic information about the sequences
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49 """
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50 self.nbSequences = 0
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51 self.reset()
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52 if self.verbosity >= 10:
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53 print "Getting information on %s." % (self.fileName)
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54
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55 nbLines = 0
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56 for line in self.handle:
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57 line = line.strip()
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58 if line == "":
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59 continue
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60 nbLines += 1
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61 if self.verbosity >= 10 and nbLines % 400000 == 0:
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62 sys.stdout.write(" %d sequences read\r" % (nbLines / 4))
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63 sys.stdout.flush()
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64 self.reset()
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65 self.nbSequences = nbLines / 4
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66 if self.verbosity >= 10:
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67 print " %d sequences read" % (self.nbSequences)
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68 print "Done."
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69
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70
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71 def parseOne(self):
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72 """
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73 Parse only one element in the file
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74 """
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75 string = ""
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76 quality = ""
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77 lineType = 0
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78
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79 for line in self.handle:
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80 line = line.strip()
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81 if lineType == 0:
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82 if line[0] != "@":
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83 raise Exception("Line '%s' should start with '@'!" % (line))
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84 name = line[1:]
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85 inSequence = True
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86 inQuality = False
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87 elif lineType == 1:
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88 string = line
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89 elif lineType == 2:
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90 if line[0] != "+":
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91 sys.exit("Line '%s' should start with '+'!" % (line))
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92 if line[1:] != name and line != "+":
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93 sys.exit("Weird difference in sequence and quality names (%s and %s) while parsing FASTQ file %s." % (name, line[1:], self.fileName))
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94 inQuality = True
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95 inSequence = False
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96 elif lineType == 3:
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97 quality = line
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98 lineType += 1
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99 if lineType == 4:
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100 sequence = Sequence(name, string)
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101 sequence.setQuality(quality)
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102 return sequence
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103
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104 return None
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