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1 import sys, os, optparse, random
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2 from galaxy_utils.sequence.fastq import fastqReader, fastqVerboseErrorReader, fastqAggregator, fastqWriter
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3
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4 def stop_err(msg):
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5 sys.stderr.write("%s\n" % msg)
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6 sys.exit()
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7
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8 def main():
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9
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10 input_filename = sys.argv[1] #a txt file
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11 input_type = sys.argv[2]
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12 output_filename = sys.argv[3] #a txt file
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13 output_type = sys.argv[4]
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14 force_quality_encoding = sys.argv[5]
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15 summarize_input = sys.argv[6] == 'summarize_input'
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16 pairedEnd_input = sys.argv[7]
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17 if pairedEnd_input == 'None':
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18 pairedEnd_input = None
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19 else:
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20 output_pairedEndFileName = sys.argv[8]
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21
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22 if force_quality_encoding == 'None':
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23 force_quality_encoding = None
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24
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25 #Parse the input txt file and read a list of fastq files
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26 file = open(input_filename, "r")
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27 lines = file.readlines()
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28 inputFileNames = []
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29 outGroomerNames = []
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30 resDirName = os.path.dirname(output_filename) + "/"
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31 #Write output txt file and define all output groomer file names
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32 outFile = open(output_filename, "w")
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33 for line in lines:
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34 tab = line.split()
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35 inputFileNames.append(tab[1])
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36 outGroomerName = resDirName + tab[0] + '_outGroomer_%s.fastq' % random.randrange(0, 10000)
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37 outGroomerNames.append(outGroomerName)
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38 outFile.write(tab[0] + '\t' + outGroomerName + '\n')
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39 outFile.close()
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40 file.close()
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41
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42 if pairedEnd_input != None:
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43 inPairedFile = open(pairedEnd_input, "r")
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44 lines = inPairedFile.readlines()
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45 inputPairedEndFileNames = []
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46 outGroomerPairedEndNames = []
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47 outPairedEndFile = open(output_pairedEndFileName, "w")
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48 for line in lines:
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49 tab = line.split()
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50 inputPairedEndFileNames.append(tab[1])
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51 outGroomerPairedEndName = resDirName + tab[0] + '_outGroomer_pairedEnd_%s.fastq' % random.randrange(0, 10000)
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52 outGroomerPairedEndNames.append(outGroomerPairedEndName)
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53 outPairedEndFile.write(tab[0] + '\t' + outGroomerPairedEndName + '\n')
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54 outPairedEndFile.close()
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55 inPairedFile.close()
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56
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57 # Write output file
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58 aggregator = fastqAggregator()
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59 for i in range(len(outGroomerNames)):
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60 out = fastqWriter( open( outGroomerNames[i], 'wb' ), format = output_type, force_quality_encoding = force_quality_encoding )
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61 read_count = None
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62 if summarize_input:
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63 reader = fastqVerboseErrorReader
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64 else:
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65 reader = fastqReader
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66 for read_count, fastq_read in enumerate( reader( open( inputFileNames[i] ), format = input_type, apply_galaxy_conventions = True ) ):
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67 if summarize_input:
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68 aggregator.consume_read( fastq_read )
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69 out.write( fastq_read )
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70 out.close()
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71
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72 if read_count is not None:
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73 print "Groomed %i %s reads into %s reads." % ( read_count + 1, input_type, output_type )
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74 if input_type != output_type and 'solexa' in [ input_type, output_type ]:
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75 print "Converted between Solexa and PHRED scores."
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76 if summarize_input:
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77 print "Based upon quality and sequence, the input data is valid for: %s" % ( ", ".join( aggregator.get_valid_formats() ) or "None" )
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78 ascii_range = aggregator.get_ascii_range()
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79 decimal_range = aggregator.get_decimal_range()
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80 print "Input ASCII range: %s(%i) - %s(%i)" % ( repr( ascii_range[0] ), ord( ascii_range[0] ), repr( ascii_range[1] ), ord( ascii_range[1] ) ) #print using repr, since \x00 (null) causes info truncation in galaxy when printed
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81 print "Input decimal range: %i - %i" % ( decimal_range[0], decimal_range[1] )
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82 else:
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83 print "No valid FASTQ reads were provided."
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84
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85
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86 # Write output pairedEnd file
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87 if pairedEnd_input != None:
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88 aggregator = fastqAggregator()
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89 for i in range(len(outGroomerPairedEndNames)):
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90 outPair = fastqWriter(open(outGroomerPairedEndNames[i], 'wb'), format = output_type, force_quality_encoding = force_quality_encoding)
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91 read_count = None
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92 if summarize_input:
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93 reader = fastqVerboseErrorReader
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94 else:
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95 reader = fastqReader
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96 for read_count, fastq_reader in enumerate(reader(open(inputPairedEndFileNames[i]), format=input_type, apply_galaxy_conventions=True)):
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97 if summarize_input:
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98 aggregator.consume_read(fastq_read)
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99 outPair.write(fastq_read)
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100 outPair.close()
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101
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102 if read_count is not None:
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103 print "Groomed %i %s reads into %s reads." % ( read_count + 1, input_type, output_type )
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104 if input_type != output_type and 'solexa' in [ input_type, output_type ]:
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105 print "Converted between Solexa and PHRED scores."
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106 if summarize_input:
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107 print "Based upon quality and sequence, the input data is valid for: %s" % ( ", ".join( aggregator.get_valid_formats() ) or "None" )
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108 ascii_range = aggregator.get_ascii_range()
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109 decimal_range = aggregator.get_decimal_range()
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110 print "Input ASCII range: %s(%i) - %s(%i)" % ( repr( ascii_range[0] ), ord( ascii_range[0] ), repr( ascii_range[1] ), ord( ascii_range[1] ) ) #print using repr, since \x00 (null) causes info truncation in galaxy when printed
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111 print "Input decimal range: %i - %i" % ( decimal_range[0], decimal_range[1] )
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112 else:
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113 print "No valid paired-end FASTQ reads were provided."
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114
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115 if __name__ == "__main__": main()
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