Mercurial > repos > yufei-luo > s_mart
comparison commons/core/parsing/FastqParser.py @ 36:44d5973c188c
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| author | m-zytnicki |
|---|---|
| date | Tue, 30 Apr 2013 15:02:29 -0400 |
| parents | 769e306b7933 |
| children |
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| 35:d94018ca4ada | 36:44d5973c188c |
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| 1 # | |
| 2 # Copyright INRA-URGI 2009-2010 | |
| 3 # | |
| 4 # This software is governed by the CeCILL license under French law and | |
| 5 # abiding by the rules of distribution of free software. You can use, | |
| 6 # modify and/ or redistribute the software under the terms of the CeCILL | |
| 7 # license as circulated by CEA, CNRS and INRIA at the following URL | |
| 8 # "http://www.cecill.info". | |
| 9 # | |
| 10 # As a counterpart to the access to the source code and rights to copy, | |
| 11 # modify and redistribute granted by the license, users are provided only | |
| 12 # with a limited warranty and the software's author, the holder of the | |
| 13 # economic rights, and the successive licensors have only limited | |
| 14 # liability. | |
| 15 # | |
| 16 # In this respect, the user's attention is drawn to the risks associated | |
| 17 # with loading, using, modifying and/or developing or reproducing the | |
| 18 # software by the user in light of its specific status of free software, | |
| 19 # that may mean that it is complicated to manipulate, and that also | |
| 20 # therefore means that it is reserved for developers and experienced | |
| 21 # professionals having in-depth computer knowledge. Users are therefore | |
| 22 # encouraged to load and test the software's suitability as regards their | |
| 23 # requirements in conditions enabling the security of their systems and/or | |
| 24 # data to be ensured and, more generally, to use and operate it in the | |
| 25 # same conditions as regards security. | |
| 26 # | |
| 27 # The fact that you are presently reading this means that you have had | |
| 28 # knowledge of the CeCILL license and that you accept its terms. | |
| 29 # | |
| 30 import sys | |
| 31 from commons.core.parsing.SequenceListParser import SequenceListParser | |
| 32 from SMART.Java.Python.structure.Sequence import Sequence | |
| 33 | |
| 34 class FastqParser(SequenceListParser): | |
| 35 """A class that reads a list of sequences in FASTQ format""" | |
| 36 | |
| 37 def __init__(self, fileName, verbosity = 0): | |
| 38 super(FastqParser, self).__init__(fileName, verbosity) | |
| 39 | |
| 40 | |
| 41 def getFileFormats(): | |
| 42 return ["fastq", "mfq"] | |
| 43 getFileFormats = staticmethod(getFileFormats) | |
| 44 | |
| 45 | |
| 46 def getInfos(self): | |
| 47 """ | |
| 48 Get some generic information about the sequences | |
| 49 """ | |
| 50 self.nbSequences = 0 | |
| 51 self.reset() | |
| 52 if self.verbosity >= 10: | |
| 53 print "Getting information on %s." % (self.fileName) | |
| 54 | |
| 55 nbLines = 0 | |
| 56 for line in self.handle: | |
| 57 line = line.strip() | |
| 58 if line == "": | |
| 59 continue | |
| 60 nbLines += 1 | |
| 61 if self.verbosity >= 10 and nbLines % 400000 == 0: | |
| 62 sys.stdout.write(" %d sequences read\r" % (nbLines / 4)) | |
| 63 sys.stdout.flush() | |
| 64 self.reset() | |
| 65 self.nbSequences = nbLines / 4 | |
| 66 if self.verbosity >= 10: | |
| 67 print " %d sequences read" % (self.nbSequences) | |
| 68 print "Done." | |
| 69 | |
| 70 | |
| 71 def parseOne(self): | |
| 72 """ | |
| 73 Parse only one element in the file | |
| 74 """ | |
| 75 string = "" | |
| 76 quality = "" | |
| 77 lineType = 0 | |
| 78 | |
| 79 for line in self.handle: | |
| 80 line = line.strip() | |
| 81 if lineType == 0: | |
| 82 if line[0] != "@": | |
| 83 raise Exception("Line '%s' should start with '@'!" % (line)) | |
| 84 name = line[1:] | |
| 85 inSequence = True | |
| 86 inQuality = False | |
| 87 elif lineType == 1: | |
| 88 string = line | |
| 89 elif lineType == 2: | |
| 90 if line[0] != "+": | |
| 91 sys.exit("Line '%s' should start with '+'!" % (line)) | |
| 92 if line[1:] != name and line != "+": | |
| 93 sys.exit("Weird difference in sequence and quality names (%s and %s) while parsing FASTQ file %s." % (name, line[1:], self.fileName)) | |
| 94 inQuality = True | |
| 95 inSequence = False | |
| 96 elif lineType == 3: | |
| 97 quality = line | |
| 98 lineType += 1 | |
| 99 if lineType == 4: | |
| 100 sequence = Sequence(name, string) | |
| 101 sequence.setQuality(quality) | |
| 102 return sequence | |
| 103 | |
| 104 return None |
