Mercurial > repos > yufei-luo > s_mart
diff SMART/Java/Python/getInfoPerCoverage.py @ 38:2c0c0a89fad7
Uploaded
author | m-zytnicki |
---|---|
date | Thu, 02 May 2013 09:56:47 -0400 |
parents | 769e306b7933 |
children |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/SMART/Java/Python/getInfoPerCoverage.py Thu May 02 09:56:47 2013 -0400 @@ -0,0 +1,167 @@ +#! /usr/bin/env python +# +# Copyright INRA-URGI 2009-2010 +# +# This software is governed by the CeCILL license under French law and +# abiding by the rules of distribution of free software. You can use, +# modify and/ or redistribute the software under the terms of the CeCILL +# license as circulated by CEA, CNRS and INRIA at the following URL +# "http://www.cecill.info". +# +# As a counterpart to the access to the source code and rights to copy, +# modify and redistribute granted by the license, users are provided only +# with a limited warranty and the software's author, the holder of the +# economic rights, and the successive licensors have only limited +# liability. +# +# In this respect, the user's attention is drawn to the risks associated +# with loading, using, modifying and/or developing or reproducing the +# software by the user in light of its specific status of free software, +# that may mean that it is complicated to manipulate, and that also +# therefore means that it is reserved for developers and experienced +# professionals having in-depth computer knowledge. Users are therefore +# encouraged to load and test the software's suitability as regards their +# requirements in conditions enabling the security of their systems and/or +# data to be ensured and, more generally, to use and operate it in the +# same conditions as regards security. +# +# The fact that you are presently reading this means that you have had +# knowledge of the CeCILL license and that you accept its terms. +# +"""Compare overlap of a transcript list and list of read, and get some info depending on the coverage""" + +import os +from optparse import OptionParser +from commons.core.parsing.SequenceListParser import * +from commons.core.writer.Gff3Writer import * +from SMART.Java.Python.mySql.MySqlConnection import * +from SMART.Java.Python.structure.TranscriptListsComparator import * +from SMART.Java.Python.misc.RPlotter import * +from SMART.Java.Python.misc.Progress import * + + +if __name__ == "__main__": + + # parse command line + description = "Get Info per Coverage v1.0.1: Get a list of information clustered by the density of the coverage on a genome. [Category: Personnal]" + + parser = OptionParser(description = description) + parser.add_option("-i", "--input1", dest="inputFileName1", action="store", type="string", help="input file 1 [compulsory] [format: file in transcript format given by -f]") + parser.add_option("-f", "--format1", dest="format1", action="store", type="string", help="format of file 1 [compulsory] [format: transcript file format]") + parser.add_option("-j", "--input2", dest="inputFileName2", action="store", type="string", help="input file 2 [compulsory] [format: file in transcript format given by -g]") + parser.add_option("-g", "--format2", dest="format2", action="store", type="string", help="format of file 2 [compulsory] [format: transcript file format]") + parser.add_option("-o", "--output", dest="output", action="store", default=None, type="string", help="output file [compulsory] [format: output file in TXT format]") + parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]") + parser.add_option("-l", "--log", dest="log", action="store", default=None, type="string", help="write a log file [format: bool] [default: false]") + (options, args) = parser.parse_args() + + logHandle = None + if options.log != None: + logHandle = open(options.log, "w") + + transcriptContainer1 = TranscriptContainer(options.inputFileName1, options.format1, options.verbosity) + transcriptContainer2 = TranscriptContainer(options.inputFileName2, options.format2, options.verbosity) + + transcriptListComparator = TranscriptListsComparator(logHandle, options.verbosity) + transcriptListComparator.restrictToStart(transcriptListComparator.REFERENCE, 10) + transcriptListComparator.getColinearOnly(True) + transcriptListComparator.computeOddsPerTranscript(True) + transcriptListComparator.setInputTranscriptContainer(transcriptListComparator.QUERY, transcriptContainer1) + transcriptListComparator.setInputTranscriptContainer(transcriptListComparator.REFERENCE, transcriptContainer2) + transcriptListComparator.compareTranscriptList() + transcriptTables = transcriptListComparator.getOutputTables() + + sizesWithIntrons = {} + sizesWithoutIntrons = {} + nbExons = {} + averageSizesWithIntrons = {} + averageSizesWithoutIntrons = {} + averageNbExons = {} + sumSizesWithIntrons = {} + sumSizesWithoutIntrons = {} + sumSizesNbExons = {} + coverages = transcriptListComparator.getOddsPerTranscript() + + progress = Progress(transcriptContainer2.getNbTranscripts(), "Reading transcript file again", options.verbosity) + for transcript in transcriptContainer2.getIterator(): + if transcript.name in coverages: + if transcript.getSizeWithIntrons() not in averageSizesWithIntrons: + averageSizesWithIntrons[transcript.getSizeWithIntrons()] = coverages[transcript.name] + else: + averageSizesWithIntrons[transcript.getSizeWithIntrons()] += coverages[transcript.name] + if transcript.getSizeWithIntrons() not in sumSizesWithIntrons: + sumSizesWithIntrons[transcript.getSizeWithIntrons()] = 1 + else: + sumSizesWithIntrons[transcript.getSizeWithIntrons()] += 1 + if transcript.getSize() not in averageSizesWithoutIntrons: + averageSizesWithoutIntrons[transcript.getSize()] = coverages[transcript.name] + else: + averageSizesWithoutIntrons[transcript.getSize()] += coverages[transcript.name] + if transcript.getSize() not in sumSizesWithoutIntrons: + sumSizesWithoutIntrons[transcript.getSize()] = 1 + else: + sumSizesWithoutIntrons[transcript.getSize()] += 1 + if transcript.getNbExons() not in averageNbExons: + averageNbExons[transcript.getNbExons()] = coverages[transcript.name] + else: + averageNbExons[transcript.getNbExons()] += coverages[transcript.name] + if transcript.getNbExons() not in sumSizesNbExons: + sumSizesNbExons[transcript.getNbExons()] = 1 + else: + sumSizesNbExons[transcript.getNbExons()] += 1 + sizesWithIntrons[transcript.name] = (transcript.getSizeWithIntrons(), coverages[transcript.name]) + sizesWithoutIntrons[transcript.name] = (transcript.getSize(), coverages[transcript.name]) + nbExons[transcript.name] = (transcript.getNbExons(), coverages[transcript.name]) + progress.inc() + progress.done() + + plotterSizeWithIntrons = RPlotter("%sWithIntrons.png" % (options.output), options.verbosity) + plotterSizeWithIntrons.setPoints(True) + plotterSizeWithIntrons.setMaximumX(10000) + plotterSizeWithIntrons.setMaximumY(1000) + plotterSizeWithIntrons.setLog("y") + plotterSizeWithIntrons.addLine(sizesWithIntrons) + plotterSizeWithIntrons.plot() + + plotterSizeWithoutIntrons = RPlotter("%sWithoutIntrons.png" % (options.output), options.verbosity) + plotterSizeWithoutIntrons.setPoints(True) + plotterSizeWithoutIntrons.setMaximumX(10000) + plotterSizeWithoutIntrons.setMaximumY(1000) + plotterSizeWithoutIntrons.setLog("y") + plotterSizeWithoutIntrons.addLine(sizesWithoutIntrons) + plotterSizeWithoutIntrons.plot() + + plotterNbExons = RPlotter("%sNbExons.png" % (options.output), options.verbosity) + plotterNbExons.setPoints(True) + plotterNbExons.addLine(nbExons) + plotterNbExons.plot() + + for element in averageSizesWithIntrons: + averageSizesWithIntrons[element] = int(float(averageSizesWithIntrons[element]) / sumSizesWithIntrons[element]) + plotterAverageSizeWithIntrons = RPlotter("%sAverageWithIntrons.png" % (options.output), options.verbosity) + plotterAverageSizeWithIntrons.setMaximumX(10000) + plotterAverageSizeWithIntrons.setMaximumY(1000) + plotterAverageSizeWithIntrons.setLog("y") + plotterAverageSizeWithIntrons.addLine(averageSizesWithIntrons) + plotterAverageSizeWithIntrons.plot() + print "min/avg/med/max sizes with introns: %d/%.2f/%.1f/%d" % Utils.getMinAvgMedMax(averageSizesWithIntrons) + + for element in averageSizesWithoutIntrons: + averageSizesWithoutIntrons[element] = int(float(averageSizesWithoutIntrons[element]) / sumSizesWithoutIntrons[element]) + plotterAverageSizeWithoutIntrons = RPlotter("%sAverageWithoutIntrons.png" % (options.output), options.verbosity) + plotterAverageSizeWithoutIntrons.setMaximumX(10000) + plotterAverageSizeWithoutIntrons.setMaximumY(1000) + plotterAverageSizeWithoutIntrons.setLog("y") + plotterAverageSizeWithoutIntrons.addLine(averageSizesWithoutIntrons) + plotterAverageSizeWithoutIntrons.plot() + print "min/avg/med/max sizes without introns: %d/%.2f/%.1f/%d" % Utils.getMinAvgMedMax(averageSizesWithoutIntrons) + + for element in averageNbExons: + averageNbExons[element] = int(float(averageNbExons[element]) / sumSizesNbExons[element]) + plotterAverageNbExons = RPlotter("%sAverageNbExons.png" % (options.output), options.verbosity) + plotterAverageNbExons.addLine(averageNbExons) + plotterAverageNbExons.plot() + print "min/avg/med/max # exons: %d/%.2f/%.1f/%d" % Utils.getMinAvgMedMax(averageNbExons) + + if options.log: + logHandle.close()