Mercurial > repos > yufei-luo > s_mart
diff SMART/Java/Python/findTss.py @ 6:769e306b7933
Change the repository level.
author | yufei-luo |
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date | Fri, 18 Jan 2013 04:54:14 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/SMART/Java/Python/findTss.py Fri Jan 18 04:54:14 2013 -0500 @@ -0,0 +1,77 @@ +#! /usr/bin/env python +# +# Copyright INRA-URGI 2009-2010 +# +# This software is governed by the CeCILL license under French law and +# abiding by the rules of distribution of free software. You can use, +# modify and/ or redistribute the software under the terms of the CeCILL +# license as circulated by CEA, CNRS and INRIA at the following URL +# "http://www.cecill.info". +# +# As a counterpart to the access to the source code and rights to copy, +# modify and redistribute granted by the license, users are provided only +# with a limited warranty and the software's author, the holder of the +# economic rights, and the successive licensors have only limited +# liability. +# +# In this respect, the user's attention is drawn to the risks associated +# with loading, using, modifying and/or developing or reproducing the +# software by the user in light of its specific status of free software, +# that may mean that it is complicated to manipulate, and that also +# therefore means that it is reserved for developers and experienced +# professionals having in-depth computer knowledge. Users are therefore +# encouraged to load and test the software's suitability as regards their +# requirements in conditions enabling the security of their systems and/or +# data to be ensured and, more generally, to use and operate it in the +# same conditions as regards security. +# +# The fact that you are presently reading this means that you have had +# knowledge of the CeCILL license and that you accept its terms. +# +"""Find TSS from short reads""" +import os +from optparse import OptionParser +from SMART.Java.Python.structure.TranscriptListsComparator import TranscriptListsComparator +from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer +from commons.core.writer.Gff3Writer import Gff3Writer + +if __name__ == "__main__": + + # parse command line + description = "Find TSS v1.0.1: Find the transcription start site of a list of transcripts. [Category: Merge]" + + parser = OptionParser(description = description) + parser.add_option("-i", "--input", dest="inputFileName", action="store", type="string", help="input file [compulsory] [format: file in transcript format given by -f]") + parser.add_option("-f", "--format", dest="format", action="store", type="string", help="format of file [compulsory] [format: transcript file format]") + parser.add_option("-o", "--output", dest="output", action="store", default=None, type="string", help="output file [compulsory] [format: output file in GFF3 format]") + parser.add_option("-n", "--normalize", dest="normalize", action="store_true", default=False, help="normalize the number of reads per cluster by the number of mappings per read [format: bool] [default: false]") + parser.add_option("-d", "--distance", dest="distance", action="store", default=10, type="int", help="distance between two reads to mark the same TSS [format: int] [default: 10]") + parser.add_option("-e", "--colinear", dest="colinear", action="store_true", default=False, help="group by strand [format: bool] [default: false]") + parser.add_option("-c", "--csv", dest="csv", action="store", default=None, type="string", help="output a CSV file in the given path [format: output file in Excel format]") + parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]") + (options, args) = parser.parse_args() + + transcriptContainer = TranscriptContainer(options.inputFileName, options.format, options.verbosity) + transcriptListComparator = TranscriptListsComparator(None, options.verbosity) + transcriptListComparator.restrictToStart(transcriptListComparator.QUERY, 1) + transcriptListComparator.setMaxDistance(options.distance) + transcriptListComparator.aggregate(True) + transcriptListComparator.computeOdds(True) + transcriptListComparator.getColinearOnly(options.colinear) + transcriptListComparator.setNormalization(options.normalize) + transcriptListComparator.setInputTranscriptContainer(transcriptListComparator.QUERY, transcriptContainer) + transcriptListComparator.setOutputWriter(Gff3Writer(options.output, options.verbosity)) + transcriptListComparator.compareTranscriptListSelfMerge() + + if options.csv != None: + csvResults = transcriptListComparator.getOddsPerTranscript() + csvFile = open(options.csv, "w") + csvFile.write("Number,Transcript\n") + for number in sorted(list(set(csvResults.values()))): + csvFile.write("%d," % (number)) + for name in csvResults: + if csvResults[name] == number: + csvFile.write("%s " % (name)) + csvFile.write("\n") + csvFile.close() +