Mercurial > repos > yufei-luo > s_mart
diff SMART/Java/Python/getReadDistribution.py @ 6:769e306b7933
Change the repository level.
author | yufei-luo |
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date | Fri, 18 Jan 2013 04:54:14 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/SMART/Java/Python/getReadDistribution.py Fri Jan 18 04:54:14 2013 -0500 @@ -0,0 +1,129 @@ +#! /usr/bin/env python +# +# Copyright INRA-URGI 2009-2010 +# +# This software is governed by the CeCILL license under French law and +# abiding by the rules of distribution of free software. You can use, +# modify and/ or redistribute the software under the terms of the CeCILL +# license as circulated by CEA, CNRS and INRIA at the following URL +# "http://www.cecill.info". +# +# As a counterpart to the access to the source code and rights to copy, +# modify and redistribute granted by the license, users are provided only +# with a limited warranty and the software's author, the holder of the +# economic rights, and the successive licensors have only limited +# liability. +# +# In this respect, the user's attention is drawn to the risks associated +# with loading, using, modifying and/or developing or reproducing the +# software by the user in light of its specific status of free software, +# that may mean that it is complicated to manipulate, and that also +# therefore means that it is reserved for developers and experienced +# professionals having in-depth computer knowledge. Users are therefore +# encouraged to load and test the software's suitability as regards their +# requirements in conditions enabling the security of their systems and/or +# data to be ensured and, more generally, to use and operate it in the +# same conditions as regards security. +# +# The fact that you are presently reading this means that you have had +# knowledge of the CeCILL license and that you accept its terms. +# +""" +Plot the data from the data files +""" +import os +from optparse import OptionParser +from commons.core.parsing.FastaParser import FastaParser +from commons.core.parsing.FastqParser import FastqParser +from SMART.Java.Python.misc.RPlotter import RPlotter +from SMART.Java.Python.misc.Progress import Progress +from SMART.Java.Python.misc import Utils + + +if __name__ == "__main__": + + # parse command line + description = "Get Read Distribution v1.0.1: Plot the number of identical reads and give the most represented. [Category: Visualization]" + + parser = OptionParser(description = description) + parser.add_option("-i", "--input", dest="inputFileName", action="store", type="string", help="input file sequence [compulsory] [format: file in sequence format given by -f]") + parser.add_option("-f", "--format", dest="format", action="store", type="string", help="format of the file [compulsory] [format: sequence file format]") + parser.add_option("-n", "--number", dest="number", action="store", default=None, type="int", help="keep the best n [format: int]") + parser.add_option("-p", "--percent", dest="percent", action="store", default=None, type="float", help="keep the best n\% [format: float]") + parser.add_option("-o", "--output", dest="outputFileName", action="store", type="string", help="output file [compulsory] [format: output files in PNG format and txt format]") + parser.add_option("-x", "--xMax", dest="xMax", action="store", default=None, type="int", help="maximum value on the x-axis to plot [format: int]") + parser.add_option("-D", "--directory", dest="working_Dir", action="store", default=os.getcwd(), type="string", help="the directory to store the results [format: directory]") + parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]") + (options, args) = parser.parse_args() + + if options.working_Dir[-1] != '/': + options.outputFileName = options.working_Dir + '/' + options.outputFileName + + if options.format == "fasta": + parser = FastaParser(options.inputFileName, options.verbosity) + elif options.format == "fastq": + parser = FastqParser(options.inputFileName, options.verbosity) + else: + raise Exception("Do not understand '%s' file format." % (options.format)) + + progress = Progress(parser.getNbSequences(), "Reading %s" % (options.inputFileName), options.verbosity) + sequences = {} + for sequence in parser.getIterator(): + sequence = sequence.sequence + if sequence not in sequences: + sequences[sequence] = 1 + else: + sequences[sequence] += 1 + progress.inc() + progress.done() + + values = sequences.values() + values.sort() + if options.percent != None: + threshold = values[int(float(options.percent) / 100 * len(values))] + elif options.number != None: + threshold = values[-options.number] + else: + threshold = 0 + + # sort by value + progress = Progress(parser.getNbSequences(), "Sorting values", options.verbosity) + sortedValues = dict([(value, []) for value in sequences.values()]) + for sequence, value in sequences.iteritems(): + sortedValues[value].append(sequence) + progress.inc() + progress.done() + + outputFileName = "%s.txt" % (options.outputFileName) + handle = open(outputFileName, "w") + progress = Progress(parser.getNbSequences(), "Writing into %s" % (outputFileName), options.verbosity) + for value in reversed(sorted(sortedValues.keys())): + if value >= threshold: + for sequence in sortedValues[value]: + handle.write("%s\t%d\n" % (sequence, value)) + progress.inc() + progress.done() + handle.close() + + line = {} + progress = Progress(len(values), "Preparing plot", options.verbosity) + for value in values: + if value not in line: + line[value] = 1 + else: + line[value] += 1 + progress.inc() + progress.done() + + plot = RPlotter("%s.png" % (options.outputFileName), options.verbosity) + plot.setFill(0) + plot.setMaximumX(options.xMax) + plot.setXLabel("# occurrences") + plot.setYLabel("# reads") + plot.addLine(line) + plot.plot() + + if options.verbosity > 0: + print "%d/%.2f/%.1f/%d occurrences" % (Utils.getMinAvgMedMax(line)) + +