Mercurial > repos > yufei-luo > s_mart
diff SMART/Java/Python/getWigDistance.py @ 6:769e306b7933
Change the repository level.
author | yufei-luo |
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date | Fri, 18 Jan 2013 04:54:14 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/SMART/Java/Python/getWigDistance.py Fri Jan 18 04:54:14 2013 -0500 @@ -0,0 +1,105 @@ +#! /usr/bin/env python +# +# Copyright INRA-URGI 2009-2010 +# +# This software is governed by the CeCILL license under French law and +# abiding by the rules of distribution of free software. You can use, +# modify and/ or redistribute the software under the terms of the CeCILL +# license as circulated by CEA, CNRS and INRIA at the following URL +# "http://www.cecill.info". +# +# As a counterpart to the access to the source code and rights to copy, +# modify and redistribute granted by the license, users are provided only +# with a limited warranty and the software's author, the holder of the +# economic rights, and the successive licensors have only limited +# liability. +# +# In this respect, the user's attention is drawn to the risks associated +# with loading, using, modifying and/or developing or reproducing the +# software by the user in light of its specific status of free software, +# that may mean that it is complicated to manipulate, and that also +# therefore means that it is reserved for developers and experienced +# professionals having in-depth computer knowledge. Users are therefore +# encouraged to load and test the software's suitability as regards their +# requirements in conditions enabling the security of their systems and/or +# data to be ensured and, more generally, to use and operate it in the +# same conditions as regards security. +# +# The fact that you are presently reading this means that you have had +# knowledge of the CeCILL license and that you accept its terms. +# +""" +Cluster the data into regions (defined by size and overlap with next region) and keep only highest peaks. +""" + +from optparse import OptionParser +from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer +from commons.core.parsing.WigParser import WigParser +from SMART.Java.Python.misc.Progress import Progress +from SMART.Java.Python.misc.RPlotter import RPlotter + + +if __name__ == "__main__": + + # parse command line + description = "Get WIG Data v1.0.2: Compute the average data around some genomic coordinates using WIG files (thus covering a large proportion of the genome). [Category: WIG Tools]" + + parser = OptionParser(description = description) + parser.add_option("-i", "--input", dest="inputFileName", action="store", type="string", help="input file [compulsory] [format: file in transcript format given by -f]") + parser.add_option("-f", "--inputFormat", dest="inputFormat", action="store", type="string", help="format of the input file [compulsory] [format: transcript file format]") + parser.add_option("-w", "--wig", dest="wig", action="store", type="string", help="wig file name [compulsory] [format: file in WIG format]") + parser.add_option("-d", "--distance", dest="distance", action="store", default=1000, type="int", help="distance around position [compulsory] [format: int] [default: 1000]") + parser.add_option("-s", "--strands", dest="strands", action="store_true", default=False, help="consider both strands separately [format: boolean] [default: False]") + parser.add_option("-o", "--output", dest="outputFileName", action="store", type="string", help="output file [compulsory] [format: output file in PNG format]") + parser.add_option("-a", "--default", dest="defaultValue", action="store", default=0.0, type="float", help="default value (when value is NA) [default: 0.0] [format: float]") + parser.add_option("-l", "--log", dest="log", action="store_true", default=False, help="use log scale for y-axis [format: boolean] [default: False]") + parser.add_option("-k", "--keep", dest="keep", action="store_true", default=False, help="keep temporary files [format: boolean] [default: False]") + parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]") + (options, args) = parser.parse_args() + + # create parsers and writers + transcriptParser = TranscriptContainer(options.inputFileName, options.inputFormat, options.verbosity) + wigParser = WigParser(options.wig) + wigParser.setStrands(options.strands) + wigParser.setDefaultValue(options.defaultValue) + + # allocate data + strands = (1, -1) if options.strands else (1, ) + values = {} + for strand in strands: + values[strand] = dict([(i, 0.0) for i in range(-options.distance, options.distance+1)]) + + # read transcripts + progress = Progress(transcriptParser.getNbTranscripts(), "Parsing %s" % (options.inputFileName), options.verbosity) + for transcript in transcriptParser.getIterator(): + transcript.removeExons() + transcript.restrictStart(2) + transcript.extendStart(options.distance) + transcript.extendEnd(options.distance-1) + theseValues = transcript.extractWigData(wigParser) + if len(strands) == 1: + theseValues = {1: theseValues} + for strand in strands: + if len(theseValues[strand]) < 2 * options.distance + 1: + theseValues[strand] = [options.defaultValue] * (2 * options.distance + 1 - len(theseValues[strand])) + theseValues[strand] + if len(theseValues[strand]) != 2 * options.distance + 1: + raise Exception("Got something wrong with the size of the WIG data concerning %s: %d found instead of %d" % (transcript, len(theseValues[strand]), 2 * options.distance + 1)) + for i in range(-options.distance, options.distance+1): + values[strand][i] += theseValues[strand][i + options.distance] + progress.inc() + progress.done() + + for strand in strands: + for i in range(-options.distance, options.distance+1): + values[strand][i] /= transcriptParser.getNbTranscripts() * strand + + # draw plot + plotter = RPlotter(options.outputFileName, options.verbosity, options.keep) + plotter.setXLabel("Distance") + plotter.setYLabel("WigValue") + for strand in strands: + plotter.addLine(values[strand]) + if options.log: + plotter.setLog("y") + plotter.plot() +