Mercurial > repos > yufei-luo > s_mart
diff SMART/Java/Python/plotRepartition.py @ 6:769e306b7933
Change the repository level.
author | yufei-luo |
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date | Fri, 18 Jan 2013 04:54:14 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/SMART/Java/Python/plotRepartition.py Fri Jan 18 04:54:14 2013 -0500 @@ -0,0 +1,128 @@ +#! /usr/bin/env python +# +# Copyright INRA-URGI 2009-2010 +# +# This software is governed by the CeCILL license under French law and +# abiding by the rules of distribution of free software. You can use, +# modify and/ or redistribute the software under the terms of the CeCILL +# license as circulated by CEA, CNRS and INRIA at the following URL +# "http://www.cecill.info". +# +# As a counterpart to the access to the source code and rights to copy, +# modify and redistribute granted by the license, users are provided only +# with a limited warranty and the software's author, the holder of the +# economic rights, and the successive licensors have only limited +# liability. +# +# In this respect, the user's attention is drawn to the risks associated +# with loading, using, modifying and/or developing or reproducing the +# software by the user in light of its specific status of free software, +# that may mean that it is complicated to manipulate, and that also +# therefore means that it is reserved for developers and experienced +# professionals having in-depth computer knowledge. Users are therefore +# encouraged to load and test the software's suitability as regards their +# requirements in conditions enabling the security of their systems and/or +# data to be ensured and, more generally, to use and operate it in the +# same conditions as regards security. +# +# The fact that you are presently reading this means that you have had +# knowledge of the CeCILL license and that you accept its terms. +# +""" +Plot the data from the data files +""" +import os +from optparse import OptionParser +from commons.core.parsing.GffParser import GffParser +from SMART.Java.Python.misc.RPlotter import RPlotter +from SMART.Java.Python.misc.Progress import Progress + + +if __name__ == "__main__": + + # parse command line + description = "Plot Repartition v1.0.1: Plot the repartition of different data on a whole genome. (This tool uses 1 input file only, the different values being stored in the tags. See documentation to know more about it.) [Category: Visualization]" + + parser = OptionParser(description = description) + parser.add_option("-i", "--input", dest="inputFileName", action="store", type="string", help="input file name [compulsory] [format: file in GFF3 format]") + parser.add_option("-n", "--names", dest="names", action="store", default=None, type="string", help="name for the tags (separated by commas and no space) [default: None] [format: string]") + parser.add_option("-o", "--output", dest="outputFileName", action="store", type="string", help="output file [compulsory] [format: output file in PNG format]") + parser.add_option("-c", "--color", dest="colors", action="store", default=None, type="string", help="color of the lines (separated by commas and no space) [format: string]") + parser.add_option("-f", "--format", dest="format", action="store", default="png", type="string", help="format of the output file [format: string] [default: png]") + parser.add_option("-r", "--normalize", dest="normalize", action="store_true", default=False, help="normalize data (when panels are different) [format: bool] [default: false]") + parser.add_option("-l", "--log", dest="log", action="store", default="", type="string", help="use log on x- or y-axis (write 'x', 'y' or 'xy') [format: string]") + parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]") + parser.add_option("-D", "--directory", dest="working_Dir", action="store", default=os.getcwd(), type="string", help="the directory to store the results [format: directory]") + (options, args) = parser.parse_args() + + strands = [1, -1] + strandToString = {1: "+", -1: "-"} + names = [None] if options.names == None else options.names.split(",") + maxs = {} + nbElements = [0 for name in names] + lines = [{} for i in range(len(names))] + if options.colors == None: + colors = [None for i in range(len(names))] + else: + colors = options.colors.split(",") + + parser = GffParser(options.inputFileName, options.verbosity) + progress = Progress(parser.getNbTranscripts(), "Reading %s" % (options.inputFileName), options.verbosity) + for transcript in parser.getIterator(): + chromosome = transcript.getChromosome() + direction = transcript.getDirection() + start = transcript.getStart() + for i, name in enumerate(names): + if chromosome not in lines[i]: + lines[i][chromosome] = dict([(strand, {}) for strand in strands]) + if chromosome not in maxs: + maxs[chromosome] = transcript.getStart() + else: + maxs[chromosome] = max(maxs[chromosome], start) + if start not in lines[i][chromosome][direction]: + lines[i][chromosome][direction][start] = 0 + thisNbElements = float(transcript.getTagValue(name)) if name != None and name in transcript.getTagNames() else 1 + lines[i][chromosome][direction][start] += thisNbElements * direction + nbElements[i] += thisNbElements + progress.inc() + progress.done() + + if options.normalize: + if options.verbosity >= 10: + print "Normalizing..." + for i, linesPerCondition in enumerate(lines): + for linesPerChromosome in linesPerCondition.values(): + for line in linesPerChromosome.values(): + for key, value in line.iteritems(): + line[key] = value / float(nbElements[i]) * max(nbElements) + if options.verbosity >= 10: + print "... done." + + progress = Progress(len(maxs.keys()), "Plotting", options.verbosity) + for chromosome in maxs: + plot = RPlotter("%s%s.%s" % (options.outputFileName, chromosome.capitalize(), options.format), options.verbosity) + plot.setLog(options.log) + plot.setImageSize(2000, 500) + plot.setFormat(options.format) + if maxs[chromosome] <= 1000: + unit = "nt." + ratio = 1.0 + elif maxs[chromosome] <= 1000000: + unit = "kb" + ratio = 1000.0 + else: + unit = "Mb" + ratio = 1000000.0 + plot.setXLabel("Position on %s (in %s)" % (chromosome.replace("_", " "), unit)) + plot.setYLabel("# reads") + plot.setLegend(True) + for i, name in enumerate(names): + for strand in strands: + correctedLine = dict([(key / ratio, value) for key, value in lines[i][chromosome][strand].iteritems()]) + if name != None: + name = "%s (%s)" % (name.replace("_", " "), strandToString[strand]) + plot.addLine(correctedLine, None, colors[i]) + plot.plot() + progress.inc() + progress.done() +