Mercurial > repos > yufei-luo > s_mart
diff SMART/Java/Python/qualToFastq.py @ 6:769e306b7933
Change the repository level.
author | yufei-luo |
---|---|
date | Fri, 18 Jan 2013 04:54:14 -0500 |
parents | |
children |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/SMART/Java/Python/qualToFastq.py Fri Jan 18 04:54:14 2013 -0500 @@ -0,0 +1,87 @@ +#! /usr/bin/env python +# +# Copyright INRA-URGI 2009-2010 +# +# This software is governed by the CeCILL license under French law and +# abiding by the rules of distribution of free software. You can use, +# modify and/ or redistribute the software under the terms of the CeCILL +# license as circulated by CEA, CNRS and INRIA at the following URL +# "http://www.cecill.info". +# +# As a counterpart to the access to the source code and rights to copy, +# modify and redistribute granted by the license, users are provided only +# with a limited warranty and the software's author, the holder of the +# economic rights, and the successive licensors have only limited +# liability. +# +# In this respect, the user's attention is drawn to the risks associated +# with loading, using, modifying and/or developing or reproducing the +# software by the user in light of its specific status of free software, +# that may mean that it is complicated to manipulate, and that also +# therefore means that it is reserved for developers and experienced +# professionals having in-depth computer knowledge. Users are therefore +# encouraged to load and test the software's suitability as regards their +# requirements in conditions enabling the security of their systems and/or +# data to be ensured and, more generally, to use and operate it in the +# same conditions as regards security. +# +# The fact that you are presently reading this means that you have had +# knowledge of the CeCILL license and that you accept its terms. +# +import sys +from optparse import OptionParser +from commons.core.parsing.SequenceListParser import SequenceListParser +from SMART.Java.Python.misc.Progress import Progress + +""" +Transform qual and fasta files to a single fastq file +""" + +if __name__ == "__main__": + + # parse command line + description = "Qual To FastQ v1.0.2: Convert a file in FASTA/Qual format to FastQ format. [Category: Conversion]" + + parser = OptionParser(description = description) + parser.add_option("-f", "--fasta", dest="fastaFileName", action="store", type="string", help="input fasta file [compulsory] [format: file in FASTA format]") + parser.add_option("-q", "--qual", dest="qualFileName", action="store", type="string", help="input qual file [compulsory] [format: file in TXT format]") + parser.add_option("-o", "--output", dest="outputFileName", action="store", default=None, type="string", help="output file [compulsory] [format: output file in FASTQ format]") + parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]") + (options, args) = parser.parse_args() + + fastaFile = open(options.fastaFileName) + qualFile = open(options.qualFileName) + fastqFile = open(options.outputFileName, "w") + + fastaLine = fastaFile.readline().strip() + qualLine = qualFile.readline().strip() + header = None + cpt = 0 + while fastaLine: + if not qualLine: + raise Exception("Qual file is shorter!") + if fastaLine[0] == ">": + header = fastaLine[1:] + if qualLine[0] != ">": + raise Exception("Discrepencies around %s!" % (header)) + fastqFile.write("@%s\n" % (header)) + else: + if qualLine[0] == ">": + raise Exception("Discrepencies around %s!" % (qualLine[1:])) + intQualities = qualLine.split() + if len(intQualities) != len(fastaLine): + raise Exception("Sizes of read and quality diverge in %s!" % (header)) + chrQualities = [chr(min(int(quality), 93) + 33) for quality in intQualities] + fastqFile.write("%s\n+\n%s\n" % (fastaLine, "".join(chrQualities))) + fastaLine = fastaFile.readline().strip() + qualLine = qualFile.readline().strip() + if cpt % 1000 == 0 and options.verbosity > 1: + sys.stdout.write("%d lines read\r" % (cpt)) + sys.stdout.flush() + cpt += 1 + if options.verbosity > 0: + print "%d lines read" % (cpt) + + if qualLine: + raise Exception("Qual file is longer!") +