--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/SMART/DiffExpAnal/fastq_groomer_parallel_unSQL.py	Mon Apr 29 03:20:15 2013 -0400
@@ -0,0 +1,168 @@
+import sys, os, optparse,shutil, random
+from commons.core.launcher.Launcher import Launcher
+from commons.core.sql.TableJobAdaptatorFactory import TableJobAdaptatorFactory
+from commons.core.utils.FileUtils import FileUtils
+
+def _map(iLauncher, cmd, cmdStart, cmdFinish ):
+	lCmds = []
+	lCmds.extend(cmd)
+	lCmdStart = []
+	lCmdStart.extend(cmdStart)
+	lCmdFinish = []
+	lCmdFinish.extend(cmdFinish)
+	return(iLauncher.prepareCommands_withoutIndentation(lCmds, lCmdStart, lCmdFinish))
+
+def splitFastQ(fileName, nbOfSeqPerBatch):
+	nbOfLinesPerFile = nbOfSeqPerBatch * 4
+	lOutput = []
+	filePrefix, fileExt = os.path.splitext(os.path.basename(fileName))
+	resDir = os.path.dirname(fileName)
+	with open(fileName) as inF:
+		fileNb = 1
+		line = inF.readline()
+		if not line or nbOfLinesPerFile == 0:
+			outFileName = "%s/%s-%s%s" %(resDir, filePrefix, fileNb, fileExt)
+			lOutput.append(outFileName)
+			f = open(outFileName, "wb")
+			shutil.copyfileobj(open(fileName, "rb"), f)
+			f.close()
+		else:
+			while line:
+				outFileName = "%s/%s-%s%s" %(resDir, filePrefix, fileNb, fileExt)
+				lOutput.append(outFileName)
+				with open(outFileName, "w") as outF:
+					lineNb = 1
+					while lineNb <= nbOfLinesPerFile and line:
+						outF.write(line)
+						line = inF.readline()
+						lineNb += 1
+				fileNb += 1
+	return lOutput
+
+def joinFastQ(dCutOut2Out):
+	for key in dCutOut2Out.keys():
+		FileUtils.catFilesFromList(dCutOut2Out[key],key, False)
+		
+def _createFastqGroomerCode(outGroomerNames, inputFileNames, input_type, output_type, force_quality_encoding, summarize_input):
+	cmd2Launch = []
+	cmd2Launch.append("log = 0")
+	cmd2Launch.append("from galaxy_utils.sequence.fastq import fastqReader, fastqVerboseErrorReader, fastqAggregator, fastqWriter")
+	cmd2Launch.append("aggregator = fastqAggregator()")
+	cmd2Launch.append("out = fastqWriter( open( '%s', 'wb' ), format = '%s', force_quality_encoding = '%s')" % (outGroomerNames,output_type,force_quality_encoding))
+	cmd2Launch.append("read_count = None")
+	if summarize_input:
+		cmd2Launch.append("reader = fastqVerboseErrorReader")
+	else:
+		cmd2Launch.append("reader = fastqReader")
+	cmd2Launch.append("for read_count, fastq_read in enumerate( reader( open( '%s' ), format = '%s', apply_galaxy_conventions = True ) ):" % (inputFileNames, input_type))
+	if summarize_input:
+		cmd2Launch.append("\taggregator.consume_read( fastq_read )")
+		cmd2Launch.append("\tout.write( fastq_read )")
+	cmd2Launch.append("out.close()")
+	cmd2Launch.append("if read_count is not None:")
+	#cmd2Launch.append("\tprint 'Groomed %s %s reads into %s reads.' % ( read_count + 1, %s, %s )" % ('%i', '%s', '%s', input_type,output_type))
+	cmd2Launch.append("\tif '%s' != '%s' and 'solexa' in [ '%s', '%s' ]:" % (input_type, output_type, input_type, output_type))
+	cmd2Launch.append("\t\tprint 'Converted between Solexa and PHRED scores.'")	
+	if summarize_input:
+		cmd2Launch.append("\tprint 'Based upon quality and sequence, the input data is valid for: %s' % ( ', '.join( aggregator.get_valid_formats() )  or 'None' )")
+		cmd2Launch.append("\tascii_range = aggregator.get_ascii_range()")
+		cmd2Launch.append("\tdecimal_range =  aggregator.get_decimal_range()")
+		cmd2Launch.append("\tprint 'Input ASCII range: %s(%i) - %s(%i)' % ( repr( ascii_range[0] ), ord( ascii_range[0] ), repr( ascii_range[1] ), ord( ascii_range[1] ) )")
+		cmd2Launch.append("\tprint 'Input decimal range: %i - %i' % ( decimal_range[0], decimal_range[1] ) ")
+	cmd2Launch.append("else:")
+	cmd2Launch.append("\tprint 'No valid FASTQ reads were provided.'")
+	cmd2Launch.append("\tlog = 255")
+	return cmd2Launch
+
+def stop_err(msg):
+	sys.stderr.write("%s\n" % msg)
+	sys.exit()
+
+def main():
+
+	input_filename = sys.argv[1]  #a txt file
+	input_type = sys.argv[2]
+	output_filename = sys.argv[3] #a txt file
+	output_type = sys.argv[4]
+	force_quality_encoding = sys.argv[5]
+	summarize_input = sys.argv[6] == 'summarize_input'
+	pairedEnd_input = sys.argv[7]
+	if pairedEnd_input == 'None':
+		pairedEnd_input = None
+	else:
+		output_pairedEndFileName = sys.argv[8]
+
+	if force_quality_encoding == 'None':
+		force_quality_encoding = None
+
+	#Parse the input txt file and read a list of fastq files
+	file = open(input_filename, "r")
+	lines = file.readlines()
+	inputFileNames = []
+	outGroomerNames = []
+	resDirName = os.path.dirname(output_filename) + "/"
+	#Write output txt file and define all output groomer file names
+	outFile = open(output_filename, "w")
+	for line in lines:
+		tab = line.split()
+		inputFileNames.append(tab[1])
+		outGroomerName = resDirName + tab[0] + '_outGroomer_%s.fastq' % random.randrange(0, 10000)
+		outGroomerNames.append(outGroomerName)
+		outFile.write(tab[0] + '\t' + outGroomerName + '\n')
+	outFile.close()
+	file.close()
+
+	if pairedEnd_input != None:
+		inPairedFile = open(pairedEnd_input, "r")
+		lines = inPairedFile.readlines()
+		inputPairedEndFileNames = []
+		outGroomerPairedEndNames = []
+		outPairedEndFile = open(output_pairedEndFileName, "w")
+		for line in lines:
+			tab = line.split()
+			inputPairedEndFileNames.append(tab[1])
+			outGroomerPairedEndName = resDirName + tab[0] + '_outGroomer_pairedEnd_%s.fastq' % random.randrange(0, 10000)
+			outGroomerPairedEndNames.append(outGroomerPairedEndName)
+			outPairedEndFile.write(tab[0] + '\t' + outGroomerPairedEndName + '\n')
+		outPairedEndFile.close()
+		inPairedFile.close()
+		
+	acronym = "fastqGroomer"
+	jobdb = TableJobAdaptatorFactory.createJobInstance()
+	iLauncher = Launcher(jobdb, os.getcwd(), "", "", os.getcwd(), os.getcwd(), "jobs", "", acronym, acronym, False, True)
+	lCmdsTuples = []	
+	dCutOut2Out = {}
+	lAllFile2remove = []
+	# Write output file
+	for i in range(len(outGroomerNames)):
+		lCutInputFile = splitFastQ(inputFileNames[i], 20000)
+		lAllFile2remove.extend(lCutInputFile)
+		lCutOutput = []
+		for cutInput in lCutInputFile:
+			cutOutput = "%s_out" % cutInput
+			lCutOutput.append(cutOutput)
+			lAllFile2remove.extend(lCutOutput)
+			cmd2Launch = _createFastqGroomerCode(cutOutput, cutInput, input_type, output_type, force_quality_encoding, summarize_input)
+			cmdStart = []
+			cmdFinish = []		
+			lCmdsTuples.append(_map(iLauncher, cmd2Launch, cmdStart, cmdFinish))
+		dCutOut2Out[outGroomerNames[i]] = lCutOutput
+		if pairedEnd_input != None:
+			lCutInputFile = splitFastQ(inputPairedEndFileNames[i], 20000)
+			lAllFile2remove.extend(lCutInputFile)
+			lCutOutput = []
+			for cutInput in lCutInputFile:
+				cutOutput = "%s_out" % cutInput
+				lCutOutput.append(cutOutput)
+				lAllFile2remove.extend(lCutOutput)
+				cmd2Launch = _createFastqGroomerCode(cutOutput, cutInput, input_type, output_type, force_quality_encoding, summarize_input)
+				cmdStart = []
+				cmdFinish = []			
+				lCmdsTuples.append(_map(iLauncher, cmd2Launch, cmdStart, cmdFinish))
+			dCutOut2Out[outGroomerPairedEndNames[i]] = 	lCutOutput		
+	iLauncher.runLauncherForMultipleJobs(acronym, lCmdsTuples, False)
+	
+	joinFastQ(dCutOut2Out)
+	FileUtils.removeFilesFromListIfExist(lAllFile2remove)
+
+if __name__ == "__main__": main()