Mercurial > repos > yufei-luo > s_mart
diff smart_toolShed/SMART/Java/Python/getNb.py @ 0:e0f8dcca02ed
Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
author | yufei-luo |
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date | Thu, 17 Jan 2013 10:52:14 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/smart_toolShed/SMART/Java/Python/getNb.py Thu Jan 17 10:52:14 2013 -0500 @@ -0,0 +1,99 @@ +#! /usr/bin/env python +# +# Copyright INRA-URGI 2009-2010 +# +# This software is governed by the CeCILL license under French law and +# abiding by the rules of distribution of free software. You can use, +# modify and/ or redistribute the software under the terms of the CeCILL +# license as circulated by CEA, CNRS and INRIA at the following URL +# "http://www.cecill.info". +# +# As a counterpart to the access to the source code and rights to copy, +# modify and redistribute granted by the license, users are provided only +# with a limited warranty and the software's author, the holder of the +# economic rights, and the successive licensors have only limited +# liability. +# +# In this respect, the user's attention is drawn to the risks associated +# with loading, using, modifying and/or developing or reproducing the +# software by the user in light of its specific status of free software, +# that may mean that it is complicated to manipulate, and that also +# therefore means that it is reserved for developers and experienced +# professionals having in-depth computer knowledge. Users are therefore +# encouraged to load and test the software's suitability as regards their +# requirements in conditions enabling the security of their systems and/or +# data to be ensured and, more generally, to use and operate it in the +# same conditions as regards security. +# +# The fact that you are presently reading this means that you have had +# knowledge of the CeCILL license and that you accept its terms. +# +"""Get the repartition of some elements (# exons per transcripts, # of repetitions of a mapping or # of transcripts in a cluster)""" + +from optparse import OptionParser +from SMART.Java.Python.structure.TranscriptContainer import TranscriptContainer +from SMART.Java.Python.misc.RPlotter import RPlotter +from SMART.Java.Python.misc.Progress import Progress +from SMART.Java.Python.misc import Utils +from math import * + +if __name__ == "__main__": + + # parse command line + description = "Get Nb v1.0.1: Get the distribution of exons per transcripts, or mapping per read, or transcript per cluster. [Category: Visualization]" + + parser = OptionParser(description = description) + parser.add_option("-i", "--input", dest="inputFileName", action="store", type="string", help="input file [compulsory] [format: file in transcript format given by -f]") + parser.add_option("-f", "--format", dest="format", action="store", type="string", help="format of the input file [compulsory] [format: transcript file format]") + parser.add_option("-o", "--output", dest="outputFileName", action="store", type="string", help="output file [compulsory] [format: output file in png format]") + parser.add_option("-q", "--query", dest="query", action="store", type="string", help="query [compulsory] (# exons, # transcripts) [format: choice (exon, transcript, cluster)]") + parser.add_option("-b", "--barplot", dest="barplot", action="store_true", default=False, help="use barplot representation [format: bool] [default: false]") + parser.add_option("-x", "--xMax", dest="xMax", action="store", default=None, type="int", help="maximum value on the x-axis to plot [format: int]") + parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [default: 1] [format: int]") + parser.add_option("-l", "--log", dest="log", action="store_true", default=False, help="write a log file [format: bool] [default: false]") + (options, args) = parser.parse_args() + + if options.query != "exon" and options.query != "transcript" and options.query != "cluster": + raise Exception("Do not understand query %s" % (options.query)) + + exonDistribution = {} + transcriptDistribution = {} + clusterDistribution = {} + + transcriptContainer = TranscriptContainer(options.inputFileName, options.format, options.verbosity) + + progress = Progress(transcriptContainer.getNbTranscripts(), "Parsing %s" % (options.inputFileName), options.verbosity) + # count the number of reads + for element in transcriptContainer.getIterator(): + if options.query == "exon": + nbExons = element.getNbExons() + exonDistribution[nbExons] = exonDistribution.get(nbExons, 0) + 1 + elif options.query == "transcript": + name = element.getName() + transcriptDistribution[name] = transcriptDistribution.get(name, 0) + 1 + elif options.query == "cluster": + nbElements = 1 if "nbElements" not in element.getTagNames() else element.getTagValue("nbElements") + clusterDistribution[nbElements] = clusterDistribution.get(nbElements, 0) + 1 + progress.inc() + progress.done() + + if options.query == "exon": + distribution = exonDistribution + elif options.query == "transcript": + distribution = {} + for name in transcriptDistribution: + distribution[transcriptDistribution[name]] = distribution.get(transcriptDistribution[name], 0) + 1 + elif options.query == "cluster": + distribution = clusterDistribution + + outputFileName = options.outputFileName + plotter = RPlotter(outputFileName, options.verbosity) + plotter.setImageSize(1000, 300) + plotter.setFill(0) + plotter.setMaximumX(options.xMax) + plotter.setBarplot(options.barplot) + plotter.addLine(distribution) + plotter.plot() + + print "min/avg/med/max: %d/%.2f/%.1f/%d" % (Utils.getMinAvgMedMax(distribution)) +