Mercurial > repos > yufei-luo > s_mart
diff smart_toolShed/SMART/Java/Python/removeEmptySequences.py @ 0:e0f8dcca02ed
Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
author | yufei-luo |
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date | Thu, 17 Jan 2013 10:52:14 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/smart_toolShed/SMART/Java/Python/removeEmptySequences.py Thu Jan 17 10:52:14 2013 -0500 @@ -0,0 +1,135 @@ +#! /usr/bin/env python +# +# Copyright INRA-URGI 2009-2010 +# +# This software is governed by the CeCILL license under French law and +# abiding by the rules of distribution of free software. You can use, +# modify and/ or redistribute the software under the terms of the CeCILL +# license as circulated by CEA, CNRS and INRIA at the following URL +# "http://www.cecill.info". +# +# As a counterpart to the access to the source code and rights to copy, +# modify and redistribute granted by the license, users are provided only +# with a limited warranty and the software's author, the holder of the +# economic rights, and the successive licensors have only limited +# liability. +# +# In this respect, the user's attention is drawn to the risks associated +# with loading, using, modifying and/or developing or reproducing the +# software by the user in light of its specific status of free software, +# that may mean that it is complicated to manipulate, and that also +# therefore means that it is reserved for developers and experienced +# professionals having in-depth computer knowledge. Users are therefore +# encouraged to load and test the software's suitability as regards their +# requirements in conditions enabling the security of their systems and/or +# data to be ensured and, more generally, to use and operate it in the +# same conditions as regards security. +# +# The fact that you are presently reading this means that you have had +# knowledge of the CeCILL license and that you accept its terms. +# +""" +Remove empty sequences from a FASTA or FASTQ file +""" + +import os, random +from optparse import OptionParser +from commons.core.parsing.FastaParser import * +from commons.core.parsing.FastqParser import * +from commons.core.writer.FastaWriter import * +from commons.core.writer.FastqWriter import * +from SMART.Java.Python.misc.Progress import * + + +class EmptySequenceRemover(object): + + def __init__(self, verbosity = 1): + self.verbosity = verbosity + self.inputFileName = None + self.parser = None + self.format = None + self.writer = None + self.forbiddenNames = {} + self.removedNames = {} + + + def setInputFileName(self, fileName, format): + self.inputFileName = fileName + self.format = format + if options.format == "fasta": + self.parser = FastaParser(self.inputFileName, self.verbosity) + elif options.format == "fastq": + self.parser = FastqParser(self.inputFileName, self.verbosity) + else: + sys.exit("Do not understand '%s' file format." % (self.format)) + + + def setOutputFileName(self, fileName): + if options.format == "fasta": + self.writer = FastaWriter("%s.mfa" % (fileName), self.verbosity) + elif options.format == "fastq": + self.writer = FastqWriter("%s.mfq" % (fileName), self.verbosity) + + + def parse(self): + progress = Progress(self.parser.getNbSequences(), "Reading sequences in %s" % (options.inputFileName), options.verbosity) + for sequence in self.parser.getIterator(): + name = sequence.name.split("/")[0] + if name not in self.forbiddenNames: + if sequence.sequence == "": + self.removedNames[name] = 1 + else: + self.writer.addSequence(sequence) + progress.inc() + progress.done() + self.writer.write() + + +if __name__ == "__main__": + + # parse command line + description = "Remove Empty Sequences v1.0.2: Remove all the empty sequences in a list. [Category: Personal]" + + parser = OptionParser(description = description) + parser.add_option("-i", "--input", dest="inputFileName", action="store", type="string", help="input file [compulsory] [format: file in sequence format given by -f]") + parser.add_option("-f", "--format", dest="format", action="store", type="string", help="format of the input file [compulsory] [format: sequence file format]") + parser.add_option("-j", "--input2", dest="inputFileName2", action="store", type="string", help="input file 2 (in case of pair end reads) [format: file in sequence format given by -f] [default: None]") + parser.add_option("-o", "--output", dest="outputFileName", action="store", default=None, type="string", help="output file [compulsory] [format: output file in format given by -f]") + parser.add_option("-p", "--output2", dest="outputFileName2", action="store", default=None, type="string", help="output file 2 (in case of pair end reads) [format: output file in sequence format given by -f] [default: None]") + parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int] [default: 1]") + parser.add_option("-l", "--log", dest="log", action="store_true", default=False, help="write a log file [format: bool] [default: false]") + (options, args) = parser.parse_args() + + if options.log: + logHandle = open("%s.log" % options.outputFileName, "w") + + remover = EmptySequenceRemover(options.verbosity) + remover.setInputFileName(options.inputFileName, options.format) + remover.setOutputFileName(options.outputFileName) + remover.parse() + removedNames = remover.removedNames + if options.log: + for name in removedNames: + logHandle.write("Removed '%s' in %s\n" % (name, options.inputFileName)) + nbSequences = remover.parser.getNbSequences() + + newRemovedNames = {} + if options.inputFileName2 != None: + remover = EmptySequenceRemover(options.verbosity) + remover.setInputFileName(options.inputFileName2, options.format) + remover.setOutputFileName(options.outputFileName2) + remover.forbiddenNames = removedNames + remover.parse() + newRemovedNames = remover.removedNames + if options.log: + for name in newRemovedNames: + logHandle.write("Removed '%s' in %s\n" % (name, options.inputFileName2)) + + remover = EmptySequenceRemover(options.verbosity) + remover.setInputFileName(options.inputFileName, options.format) + remover.setOutputFileName(options.outputFileName) + remover.forbiddenNames = newRemovedNames + remover.parse() + + nbRemoved = len(removedNames.keys()) + len(newRemovedNames.keys()) + print "%d over %d sequences are empty (%.2f%%)." % (nbRemoved, nbSequences, float(nbRemoved) / nbSequences * 100)