Mercurial > repos > yufei-luo > s_mart
diff smart_toolShed/SMART/Java/Python/updateQual.py @ 0:e0f8dcca02ed
Uploaded S-MART tool. A toolbox manages RNA-Seq and ChIP-Seq data.
author | yufei-luo |
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date | Thu, 17 Jan 2013 10:52:14 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/smart_toolShed/SMART/Java/Python/updateQual.py Thu Jan 17 10:52:14 2013 -0500 @@ -0,0 +1,86 @@ +#! /usr/bin/env python +# +# Copyright INRA-URGI 2009-2010 +# +# This software is governed by the CeCILL license under French law and +# abiding by the rules of distribution of free software. You can use, +# modify and/ or redistribute the software under the terms of the CeCILL +# license as circulated by CEA, CNRS and INRIA at the following URL +# "http://www.cecill.info". +# +# As a counterpart to the access to the source code and rights to copy, +# modify and redistribute granted by the license, users are provided only +# with a limited warranty and the software's author, the holder of the +# economic rights, and the successive licensors have only limited +# liability. +# +# In this respect, the user's attention is drawn to the risks associated +# with loading, using, modifying and/or developing or reproducing the +# software by the user in light of its specific status of free software, +# that may mean that it is complicated to manipulate, and that also +# therefore means that it is reserved for developers and experienced +# professionals having in-depth computer knowledge. Users are therefore +# encouraged to load and test the software's suitability as regards their +# requirements in conditions enabling the security of their systems and/or +# data to be ensured and, more generally, to use and operate it in the +# same conditions as regards security. +# +# The fact that you are presently reading this means that you have had +# knowledge of the CeCILL license and that you accept its terms. +# +"""Update a .qual file given a .fasta file""" + +from optparse import OptionParser +from commons.core.parsing.FastaParser import * +from SMART.Java.Python.misc.Progress import * + + +if __name__ == "__main__": + + # parse command line + description = "Update Qual v1.0.1: Remove the sequence in a Qual file which are not in the corresponding Fasta file. [Category: Personnal]" + + parser = OptionParser(description = description) + parser.add_option("-f", "--fasta", dest="fastaFile", action="store", type="string", help="fasta file [compulsory] [format: file in FASTA format]") + parser.add_option("-q", "--qual", dest="qualFile", action="store", type="string", help="qual file [compulsory] [format: file in QUAL format]") + parser.add_option("-o", "--output", dest="outputFile", action="store", type="string", help="output file [compulsory] [format: output file in QUAL format]") + parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]") + (options, args) = parser.parse_args() + + parser = SequenceListParser(options.fastaFile, options.verbosity) + nbSequences = parser.getNbSequences() + progress = Progress(nbSequences, "Parsing file %s" % (options.fastaFile), options.verbosity) + qualHandle = open(options.qualFile) + outputHandle = open(options.outputFile, "w") + nbRefused = 0 + nbTotal = 0 + + names = [] + while parser.getNextSequence(): + sequence = parser.getCurrentSequence() + nbTotal += 1 + + found = False + name = None + for line in qualHandle: + line = line.strip() + if line[0] == ">": + name = line[1:] + if name == sequence.name: + found = True + else: + nbRefused += 1 + else: + if found: + outputHandle.write(">%s\n%s\n" % (name, line)) + found = False + name = None + break + progress.inc() + progress.done() + + + outputHandle.close() + qualHandle.close() + + print "%d out of %d are refused (%f%%)" % (nbRefused, nbTotal, (float(nbRefused) / nbTotal * 100))