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view SMART/Java/Python/fastqToFasta.py @ 6:769e306b7933
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author | yufei-luo |
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date | Fri, 18 Jan 2013 04:54:14 -0500 |
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#! /usr/bin/env python # # Copyright INRA-URGI 2009-2010 # # This software is governed by the CeCILL license under French law and # abiding by the rules of distribution of free software. You can use, # modify and/ or redistribute the software under the terms of the CeCILL # license as circulated by CEA, CNRS and INRIA at the following URL # "http://www.cecill.info". # # As a counterpart to the access to the source code and rights to copy, # modify and redistribute granted by the license, users are provided only # with a limited warranty and the software's author, the holder of the # economic rights, and the successive licensors have only limited # liability. # # In this respect, the user's attention is drawn to the risks associated # with loading, using, modifying and/or developing or reproducing the # software by the user in light of its specific status of free software, # that may mean that it is complicated to manipulate, and that also # therefore means that it is reserved for developers and experienced # professionals having in-depth computer knowledge. Users are therefore # encouraged to load and test the software's suitability as regards their # requirements in conditions enabling the security of their systems and/or # data to be ensured and, more generally, to use and operate it in the # same conditions as regards security. # # The fact that you are presently reading this means that you have had # knowledge of the CeCILL license and that you accept its terms. # """Convert a FASTQ file to a FASTA file""" import os import sys from optparse import OptionParser from SMART.Java.Python.misc.RPlotter import RPlotter from SMART.Java.Python.misc.Progress import Progress from math import * if __name__ == "__main__": # parse command line description = "FastQ to FastA v1.0.1: Convert a FastQ file into a FastA file. [Category: Personnal]" parser = OptionParser(description = description) parser.add_option("-i", "--input", dest="inputFileName", action="store", type="string", help="input file [compulsory] [format: file in FASTQ format]") parser.add_option("-o", "--output", dest="outputFileName", action="store", type="string", help="output file [compulsory] [format: output file in FASTA format]") parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [default: 1] [format: int]") (options, args) = parser.parse_args() inputFile = open(options.inputFileName) outputFastaFile = open(options.outputFileName, "w") inSequenceName = False inQualityName = False inSequence = False inQuality = True sequenceName = None lineNumber = 1 for line in inputFile: if inSequenceName: inSequence = True inSequenceName = False elif inQualityName: inQuality = True inQualityName = False elif inSequence: inQualityName = True inSequence = False elif inQuality: inSequenceName = True inQuality = False else: sys.exit("Error! Do not in which section I am (line is %d)" % (lineNumber)) line = line.strip() if inSequenceName: if line[0] != "@": sys.exit("Error! Sequence name '%s' does not start with '@' (line is %d)" % (line, lineNumber)) sequenceName = line[1:] outputFastaFile.write(">%s\n" % (sequenceName)) elif inQualityName: if line[0] != "+": sys.exit("Error! Quality name '%s' does not start with '+' (line is %d)" % (line, lineNumber)) if len(line) > 1 and sequenceName != line[1:]: sys.exit("Names in sequence and qual are different (%s, %s) (line is %d)" % (sequenceName, line[1:], lineNumber)) elif inSequence: outputFastaFile.write("%s\n" % (line)) elif inQuality: pass lineNumber += 1 inputFile.close() outputFastaFile.close()