Mercurial > repos > yufei-luo > s_mart
view SMART/Java/Python/plotRepartition.py @ 18:94ab73e8a190
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author | m-zytnicki |
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date | Mon, 29 Apr 2013 03:20:15 -0400 |
parents | 769e306b7933 |
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#! /usr/bin/env python # # Copyright INRA-URGI 2009-2010 # # This software is governed by the CeCILL license under French law and # abiding by the rules of distribution of free software. You can use, # modify and/ or redistribute the software under the terms of the CeCILL # license as circulated by CEA, CNRS and INRIA at the following URL # "http://www.cecill.info". # # As a counterpart to the access to the source code and rights to copy, # modify and redistribute granted by the license, users are provided only # with a limited warranty and the software's author, the holder of the # economic rights, and the successive licensors have only limited # liability. # # In this respect, the user's attention is drawn to the risks associated # with loading, using, modifying and/or developing or reproducing the # software by the user in light of its specific status of free software, # that may mean that it is complicated to manipulate, and that also # therefore means that it is reserved for developers and experienced # professionals having in-depth computer knowledge. Users are therefore # encouraged to load and test the software's suitability as regards their # requirements in conditions enabling the security of their systems and/or # data to be ensured and, more generally, to use and operate it in the # same conditions as regards security. # # The fact that you are presently reading this means that you have had # knowledge of the CeCILL license and that you accept its terms. # """ Plot the data from the data files """ import os from optparse import OptionParser from commons.core.parsing.GffParser import GffParser from SMART.Java.Python.misc.RPlotter import RPlotter from SMART.Java.Python.misc.Progress import Progress if __name__ == "__main__": # parse command line description = "Plot Repartition v1.0.1: Plot the repartition of different data on a whole genome. (This tool uses 1 input file only, the different values being stored in the tags. See documentation to know more about it.) [Category: Visualization]" parser = OptionParser(description = description) parser.add_option("-i", "--input", dest="inputFileName", action="store", type="string", help="input file name [compulsory] [format: file in GFF3 format]") parser.add_option("-n", "--names", dest="names", action="store", default=None, type="string", help="name for the tags (separated by commas and no space) [default: None] [format: string]") parser.add_option("-o", "--output", dest="outputFileName", action="store", type="string", help="output file [compulsory] [format: output file in PNG format]") parser.add_option("-c", "--color", dest="colors", action="store", default=None, type="string", help="color of the lines (separated by commas and no space) [format: string]") parser.add_option("-f", "--format", dest="format", action="store", default="png", type="string", help="format of the output file [format: string] [default: png]") parser.add_option("-r", "--normalize", dest="normalize", action="store_true", default=False, help="normalize data (when panels are different) [format: bool] [default: false]") parser.add_option("-l", "--log", dest="log", action="store", default="", type="string", help="use log on x- or y-axis (write 'x', 'y' or 'xy') [format: string]") parser.add_option("-v", "--verbosity", dest="verbosity", action="store", default=1, type="int", help="trace level [format: int]") parser.add_option("-D", "--directory", dest="working_Dir", action="store", default=os.getcwd(), type="string", help="the directory to store the results [format: directory]") (options, args) = parser.parse_args() strands = [1, -1] strandToString = {1: "+", -1: "-"} names = [None] if options.names == None else options.names.split(",") maxs = {} nbElements = [0 for name in names] lines = [{} for i in range(len(names))] if options.colors == None: colors = [None for i in range(len(names))] else: colors = options.colors.split(",") parser = GffParser(options.inputFileName, options.verbosity) progress = Progress(parser.getNbTranscripts(), "Reading %s" % (options.inputFileName), options.verbosity) for transcript in parser.getIterator(): chromosome = transcript.getChromosome() direction = transcript.getDirection() start = transcript.getStart() for i, name in enumerate(names): if chromosome not in lines[i]: lines[i][chromosome] = dict([(strand, {}) for strand in strands]) if chromosome not in maxs: maxs[chromosome] = transcript.getStart() else: maxs[chromosome] = max(maxs[chromosome], start) if start not in lines[i][chromosome][direction]: lines[i][chromosome][direction][start] = 0 thisNbElements = float(transcript.getTagValue(name)) if name != None and name in transcript.getTagNames() else 1 lines[i][chromosome][direction][start] += thisNbElements * direction nbElements[i] += thisNbElements progress.inc() progress.done() if options.normalize: if options.verbosity >= 10: print "Normalizing..." for i, linesPerCondition in enumerate(lines): for linesPerChromosome in linesPerCondition.values(): for line in linesPerChromosome.values(): for key, value in line.iteritems(): line[key] = value / float(nbElements[i]) * max(nbElements) if options.verbosity >= 10: print "... done." progress = Progress(len(maxs.keys()), "Plotting", options.verbosity) for chromosome in maxs: plot = RPlotter("%s%s.%s" % (options.outputFileName, chromosome.capitalize(), options.format), options.verbosity) plot.setLog(options.log) plot.setImageSize(2000, 500) plot.setFormat(options.format) if maxs[chromosome] <= 1000: unit = "nt." ratio = 1.0 elif maxs[chromosome] <= 1000000: unit = "kb" ratio = 1000.0 else: unit = "Mb" ratio = 1000000.0 plot.setXLabel("Position on %s (in %s)" % (chromosome.replace("_", " "), unit)) plot.setYLabel("# reads") plot.setLegend(True) for i, name in enumerate(names): for strand in strands: correctedLine = dict([(key / ratio, value) for key, value in lines[i][chromosome][strand].iteritems()]) if name != None: name = "%s (%s)" % (name.replace("_", " "), strandToString[strand]) plot.addLine(correctedLine, None, colors[i]) plot.plot() progress.inc() progress.done()