Mercurial > repos > yusuf > depth_report
diff DepthReports.xml @ 0:6b2e640c8c6d
initial commit
author | Yusuf Ali <ali@yusuf.email> |
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date | Wed, 25 Mar 2015 13:31:40 -0600 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/DepthReports.xml Wed Mar 25 13:31:40 2015 -0600 @@ -0,0 +1,43 @@ +<?xml version="1.0"?> + +<tool id="depth_reports_1" name="Compute depth of sequencing coverage"> + <description>over targeted regions in a genome</description> + <version_string>depth_reports -v</version_string> + <command interpreter="perl">depth_reports $__tool_data_path__ -q $input_bam ${target_bed}.bed $out_summary_table $out_poor_coverage_bed $out_depth_graph $out_depth_table $out_mapped_percentile_table $dud_bed</command> + <inputs> + <param format="bam" name="input_bam" type="data" label="Mapped alignment file"/> + <param name="target_bed" type="select" display="radio" dynamic_options="kit_fileOptions()" label="The enrichment (capture) kit used for the sequencing experiment"/> + <param format="bed" name="dud_bed" type="data" label="Targeted regions to ignore (known duds)" optional="true" default="/dev/null"/> + </inputs> + <outputs> + <data name="out_summary_table" format="achri_depth_summary_table" label="Depth of coverage summary (mean, median, etc.)"/> + <data name="out_poor_coverage_bed" format="bed" label="Low coverage targeted regions"/> + <data name="out_depth_graph" format="png" label="Cumulative depth of coverage graph"/> + <data name="out_depth_table" format="tabular" label="Number of bases at each depth of coverage"/> + <data name="out_mapped_percentile_table" format="tabular" label="Mapped read depth percentiles"/> + </outputs> + + <!-- the following code populates the selection from the public capture kit BED datasets available in the local Galaxy installation --> + <code file="capture_kits.py"/> + + <tests> + <test> + <param name="input_bam" value="depth_test.bam" ftype="bam"/> + <param name="target_bed" value="brcas.bed" ftype="bed"/> + <output name="out_summary_table"> + <assert_contents> + <has_text text="targeted nucleotide bases: 155091"/> + <has_text text="bases mapped to targeted regions: 11473773"/> + <has_text text="bases with less than 20-fold coverage: 19046"/> + </assert_contents> + </output> + <output name="out_depth_graph" ftype="png"/> + </test> + </tests> + + <help> +This tool reports several statistics describing the depth of coverage of a next-gen sequencing run over its targeted reference genome. +These results can be used to assess the quality of the sequencing for SNP calls, etc. + </help> + +</tool>