Mercurial > repos > yusuf > miseq_bam_variants
diff MiSeqBAMtoVariants.xml @ 0:1a23ea467feb default tip
intial commit
author | Yusuf Ali <ali@yusuf.email> |
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date | Thu, 26 Mar 2015 09:36:17 -0600 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/MiSeqBAMtoVariants.xml Thu Mar 26 09:36:17 2015 -0600 @@ -0,0 +1,27 @@ +<?xml version="1.0"?> + +<tool id="call_miseq_vars" name="Call miSeq variants"> + <description>using a BAM file</description> + <version_string>echo 1.0.0</version_string> + <command interpreter="perl">call_miseq_variants $__tool_data_path__ geno . $inputBAM ${targetBED}.bed ${ref_genome}.bed ${ref_genome}.fa 12</command> + <inputs> + <param format="bam" name="inputBAM" type="data" label="Source BAM (mapped and realigned reads) file"/> + <param name="ref_genome" type="genomebuild" label="Reference genome" help="against which the reads were aligned"/> + <param name="targetBED" type="select" display="radio" dynamic_options="kit_fileOptions()" label="The enrichment (capture) kit used for the sequencing experiment"/> + </inputs> + <outputs> + <data format="achri_snp_table" label="Confident genotypes" name="confident_genotypes" from_work_dir="geno.combined.collapsed.confident.hgvs.txt"/> + <data format="achri_snp_table" label="Marginal genotypes" name="marginal_genotypes" from_work_dir="geno.combined.collapsed.marginal.hgvs.txt"/> + <data format="text" label="Genotyping log" name="genotyping log" from_work_dir="geno.call_variants.log.txt"/> + </outputs> + + <!-- the following code populates the selection from the public dbSNP datasets available in the local Galaxy installation --> + <code file="capture_kits.py"/> + <tests/> + + <help> +This tool runs a BAM file against multiple genotypers (FreeBayes 0.8.7, GATKHaplotypeCaller 3.1) and generates a consensus (confident) table of variant calls, +including caveats about haplotype phase inconsistencies and other potential false positive indicators. + </help> + +</tool>