diff MiSeqBAMtoVariants.xml @ 0:1a23ea467feb default tip

intial commit
author Yusuf Ali <ali@yusuf.email>
date Thu, 26 Mar 2015 09:36:17 -0600
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/MiSeqBAMtoVariants.xml	Thu Mar 26 09:36:17 2015 -0600
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+<?xml version="1.0"?>
+
+<tool id="call_miseq_vars" name="Call miSeq variants">
+  <description>using a BAM file</description>
+  <version_string>echo 1.0.0</version_string>
+  <command interpreter="perl">call_miseq_variants $__tool_data_path__ geno . $inputBAM ${targetBED}.bed ${ref_genome}.bed ${ref_genome}.fa 12</command>
+  <inputs>
+    <param format="bam" name="inputBAM" type="data" label="Source BAM (mapped and realigned reads) file"/>
+    <param name="ref_genome" type="genomebuild" label="Reference genome" help="against which the reads were aligned"/>
+    <param name="targetBED" type="select" display="radio" dynamic_options="kit_fileOptions()" label="The enrichment (capture) kit used for the sequencing experiment"/>
+  </inputs>
+  <outputs>
+    <data format="achri_snp_table" label="Confident genotypes" name="confident_genotypes" from_work_dir="geno.combined.collapsed.confident.hgvs.txt"/>
+    <data format="achri_snp_table" label="Marginal genotypes" name="marginal_genotypes" from_work_dir="geno.combined.collapsed.marginal.hgvs.txt"/>
+    <data format="text" label="Genotyping log" name="genotyping log" from_work_dir="geno.call_variants.log.txt"/>
+  </outputs>
+
+  <!-- the following code populates the  selection from the public dbSNP datasets available in the local Galaxy installation -->
+  <code file="capture_kits.py"/>
+  <tests/>
+
+  <help>
+This tool runs a BAM file against multiple genotypers (FreeBayes 0.8.7, GATKHaplotypeCaller 3.1) and generates a consensus (confident) table of variant calls, 
+including caveats about haplotype phase inconsistencies and other potential false positive indicators.
+ </help>
+
+</tool>