Mercurial > repos > artbio > artbio_bam_cleaning
annotate artbio_bam_cleaning.xml @ 6:999c2b871f36 draft
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
author | artbio |
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date | Wed, 07 Apr 2021 01:31:51 +0000 |
parents | ebdaf5b3d6a7 |
children | 745f529127b8 |
rev | line source |
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6
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
5
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1 <tool id="artbio_bam_cleaning" name="ARTbio bam cleaning" version="1.6+galaxy6"> |
0
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
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2 <description> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
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changeset
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3 on flags and PCR Duplicates and MD recalibration |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
changeset
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4 </description> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
changeset
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5 <macros> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
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6 <import>macro.xml</import> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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7 </macros> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
changeset
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8 <requirements> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
changeset
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9 <requirement type="package" version="1.6">samtools</requirement> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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10 <requirement type="package" version="0.7.1">sambamba</requirement> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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11 <requirement type="package" version="1.3.2">freebayes</requirement> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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12 </requirements> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
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13 <stdio> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
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14 <exit_code range="1:" level="fatal" description="Error occured" /> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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15 </stdio> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
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16 <command detect_errors="exit_code"><![CDATA[ |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
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17 @pipefail@ |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
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18 @set_fasta_index@ |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
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19 #set input_base = 'input' |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
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20 ln -f -s $input_bam.metadata.bam_index input.bam.bai && |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
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21 ln -s $input_bam input.bam && |
1
b550841f568b
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 2d441fd84bf17c0899d0b57ea35c84cb83b77119"
artbio
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22 sambamba view -h -t \${GALAXY_SLOTS:-2} --filter='mapping_quality >= 1 and not(unmapped) and not(mate_is_unmapped)' -f 'bam' $input_base".bam" |
4
15ada8e1580a
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 13e2d0837000c2557f9590aa861a9fd5bc16cbcc"
artbio
parents:
3
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23 #if $skip_rmdup == 'no': |
15ada8e1580a
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 13e2d0837000c2557f9590aa861a9fd5bc16cbcc"
artbio
parents:
3
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24 | samtools rmdup -s - - | tee $input_base".filt1.dedup.bam" |
15ada8e1580a
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 13e2d0837000c2557f9590aa861a9fd5bc16cbcc"
artbio
parents:
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25 #end if |
15ada8e1580a
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 13e2d0837000c2557f9590aa861a9fd5bc16cbcc"
artbio
parents:
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26 | bamleftalign --fasta-reference reference.fa -c --max-iterations "5" - |
5
ebdaf5b3d6a7
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit b846085c8b8e29bb067a3cdcb90355117aeebf42"
artbio
parents:
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27 | samtools calmd -C 50 -b -@ \${GALAXY_SLOTS:-2} - reference.fa |
6
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
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28 #if $skip_laststep == 'yes': |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
5
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29 > $calmd |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
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30 #else if $skip_laststep == 'no': |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
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31 | tee $calmd |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
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32 | sambamba view -h -t \${GALAXY_SLOTS:-2} --filter='mapping_quality <= 254' -f 'bam' /dev/stdin > $fullfilter |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
5
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33 #end if |
0
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
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34 ]]></command> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
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35 <inputs> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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36 <expand macro="reference_source_conditional" /> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
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37 <param name="input_bam" type="data" format="bam" label="BAM or SAM file to process"/> |
6
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
5
diff
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38 <param name="skip_rmdup" type="select" label="skip remove pcr duplicate step ?" display="radio" |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
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39 help="useful if duplicates are already marked by other tools"> |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
5
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40 <option value="no" selected="true">No</option> |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
5
diff
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41 <option value="yes">Yes</option> |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
5
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42 </param> |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
5
diff
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43 <param name="skip_laststep" type="select" label="skip last samtool view filter ?" display="radio" |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
5
diff
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44 help="Only generate the calMD output"> |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
5
diff
changeset
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45 <option value="no" selected="true">No</option> |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
5
diff
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46 <option value="yes">Yes</option> |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
5
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47 </param> |
0
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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48 </inputs> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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49 <outputs> |
3
a1462dd52f17
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit b022da54be013bf593ad96de6e7bd8b9e6bb5523"
artbio
parents:
2
diff
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50 <data name="calmd" format="bam" label="CalMD filter (for lumpy-smoove)" /> |
6
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
5
diff
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51 <data name="fullfilter" format="bam" label="Full filtering (for somatic-varscan)"> |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
5
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52 <filter>skip_laststep == "no"</filter> |
999c2b871f36
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 587b99e907726b20f0cdd978be8f9ed257a68243"
artbio
parents:
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53 </data> |
0
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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54 </outputs> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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55 <tests> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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56 <test> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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57 <param name="input_bam" value="match_chr21_DBA_974.bam" ftype="bam" /> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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58 <param name="reference_source_selector" value="history" /> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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59 <param name="ref_file" value="chr21.fa" /> |
4
15ada8e1580a
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 13e2d0837000c2557f9590aa861a9fd5bc16cbcc"
artbio
parents:
3
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60 <output name="calmd" file="calmd.bam" ftype="bam" /> |
15ada8e1580a
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 13e2d0837000c2557f9590aa861a9fd5bc16cbcc"
artbio
parents:
3
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61 <output name="fullfilter" file="full.bam" ftype="bam" /> |
15ada8e1580a
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 13e2d0837000c2557f9590aa861a9fd5bc16cbcc"
artbio
parents:
3
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62 </test> |
15ada8e1580a
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 13e2d0837000c2557f9590aa861a9fd5bc16cbcc"
artbio
parents:
3
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63 <test> |
15ada8e1580a
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 13e2d0837000c2557f9590aa861a9fd5bc16cbcc"
artbio
parents:
3
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64 <param name="input_bam" value="match_chr21_DBA_974.bam" ftype="bam" /> |
15ada8e1580a
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 13e2d0837000c2557f9590aa861a9fd5bc16cbcc"
artbio
parents:
3
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65 <param name="reference_source_selector" value="history" /> |
15ada8e1580a
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 13e2d0837000c2557f9590aa861a9fd5bc16cbcc"
artbio
parents:
3
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66 <param name="skip_rmdup" value="yes" /> |
15ada8e1580a
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 13e2d0837000c2557f9590aa861a9fd5bc16cbcc"
artbio
parents:
3
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67 <param name="ref_file" value="chr21.fa" /> |
15ada8e1580a
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 13e2d0837000c2557f9590aa861a9fd5bc16cbcc"
artbio
parents:
3
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68 <output name="calmd" file="normdup_calmd.bam" ftype="bam" /> |
15ada8e1580a
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 13e2d0837000c2557f9590aa861a9fd5bc16cbcc"
artbio
parents:
3
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69 <output name="fullfilter" file="normdup_full.bam" ftype="bam" /> |
1
b550841f568b
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit 2d441fd84bf17c0899d0b57ea35c84cb83b77119"
artbio
parents:
0
diff
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70 </test> |
0
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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71 </tests> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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72 <help> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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73 ARTbio bam cleaning overview |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
artbio
parents:
diff
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74 ============================ |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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75 |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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76 This tool is wrapping several cleaning steps to produce bam files suitable for subsequent |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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77 analyses with lumpy-smoove (or other large structural variation callers) or with |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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78 somatic-varscan (or small structural variation callers) |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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79 |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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80 |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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81 Workflow |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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82 ============= |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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83 |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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84 The tool is using the following command line for filtering: |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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85 |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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86 :: |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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87 |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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88 sambamba view -h -t 8 --filter='mapping_quality >= 1 and not(unmapped) and not(mate_is_unmapped)' -f 'bam' $input_base".bam" |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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89 | samtools rmdup - - |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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90 |tee $input_base".filt1.dedup.bam" | bamleftalign --fasta-reference reference.fa -c --max-iterations "5" - |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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91 | samtools calmd -C 50 -b -@ 4 - reference.fa > $input_base".filt1.dedup.bamleft.calmd.bam" ; |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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92 sambamba view -h -t 8 --filter='mapping_quality <= 254' -f 'bam' -o $input_base".filt1.dedup.bamleft.calmd.filt2.bam" $input_base".filt1.dedup.bamleft.calmd.bam" |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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93 |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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94 Purpose |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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95 -------- |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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96 |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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97 This "workflow" tool was generated in order to limit the number of ``python metadata/set.py`` jobs |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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98 which occur at each step of standard galaxy workflows. Indeed, these jobs are poorly optimized and may last considerable |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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99 amounts of time when datasets are large, at each step, lowering the overall performance of the workflow. |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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100 |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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101 </help> |
65d6d2b554b3
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102 <citations> |
65d6d2b554b3
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103 <citation type="doi">10.1371/journal.pone.0168397</citation> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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104 </citations> |
65d6d2b554b3
"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit adfad19ff505ac7baa3688997bfa9f64243aaace"
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105 </tool> |