Mercurial > repos > artbio > bamparse
diff bamparse.xml @ 0:2a1a2bc6ae8b draft
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/bamparse commit 4e42cba873625fad03423e65dfffbf4afa91598c
author | artbio |
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date | Fri, 13 Oct 2017 02:59:36 -0400 |
parents | |
children | ae9ea0488850 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/bamparse.xml Fri Oct 13 02:59:36 2017 -0400 @@ -0,0 +1,75 @@ +<tool id="bamparse" name="Count alignments" version="2.0.0"> + <description>in a BAM file</description> + <requirements> + <requirement type="package" version="1.1.2">bowtie</requirement> + <requirement type="package" version="1.11.2">numpy</requirement> + <requirement type="package" version="0.11.2.1">pysam</requirement> + </requirements> + <stdio> + <exit_code range="1:" level="fatal" description="Tool exception" /> + </stdio> + <command detect_errors="exit_code"><![CDATA[ + mkdir outputdir && + #for $file in $input_list + samtools index '$file' && + #end for + python $__tool_directory__/bamparse.py + --alignments + #for $file in $input_list + '$file' + #end for + --labels + #for $file in $input_list + '$file.element_identifier' + #end for + --polarity '$polarity' + --number '$output_option' + ]]></command> + <inputs> + <param name="input_list" type="data" format="bam" label="Select multiple alignments to parse" multiple="true"/> + <param name="polarity" type="select" label="how to count sense and antisense reads"> + <option value="both">count both sense and antisense reads</option> + <option value="sense">count only sense reads</option> + <option value="antisense">count only antisense reads</option> + </param> + <param name="output_option" type="select" display="radio" label="Select the number of files for results" + help="Results can be returned either as a single multi-column table or in separate two-column + datasets (useful for DESeq subsequent analysis)"> + <option value="unique">A multi-column table</option> + <option value="multiple">One separate two-columns dataset per input bam alignment</option> + </param> + </inputs> + <outputs> + <data name="output" format="tabular" label="Counts"> + <discover_datasets pattern="(?P<designation>.*)\.tabular" ext="tabular" visible="true" assign_primary_output="true" directory="outputdir"/> + </data> + </outputs> + <help> + +**What it does** + +Counts the number of reads aligned to each reference (@SN, reference NAME) in one or +several BAM alignments. + +Sense, antisense or both sense and antisense alignments can be counted + +The library labels in the returned count table are taken from the input bam datasets +names in the Galaxy history. + </help> + <tests> + <test> + <param name="input_list" value="alignment1.bam,alignment2.bam" ftype="tabular" /> + <param name="polarity" value="both" /> + <param name="output_option" value="unique" /> + <output name="output" ftype="tabular" file="table.tabular" /> + </test> + <test> + <param name="input_list" value="alignment1.bam,alignment2.bam" ftype="tabular" /> + <param name="polarity" value="both" /> + <param name="output_option" value="multiple" /> + <output name="output" ftype="tabular" file="table0.tabular"> + <discovered_dataset designation="table1" ftype="tabular" file="table1.tabular" /> + </output> + </test> + </tests> +</tool>