Mercurial > repos > azomics > ggcyto_1d_density_plots
annotate FCS1Dplotggcyto.xml @ 0:b73fc4860906 draft default tip
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
author | azomics |
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date | Mon, 22 Jun 2020 17:53:11 -0400 |
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b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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changeset
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1 <tool id="ggcyto_1d_density_plots" name="Generate 1D density plots" version="1.1+galaxy0"> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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2 <description>for FCS file</description> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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3 <requirements> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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4 <requirement type="package" version="1.14.0">bioconductor-ggcyto</requirement> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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5 <!-- to fix https://github.com/RGLab/ggcyto/issues/64 and not able to install ggcyto 1.16.0--> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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changeset
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6 <requirement type="package" version="3.2.1">r-ggplot2</requirement> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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changeset
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7 </requirements> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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8 <stdio> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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changeset
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9 <exit_code range="1:" /> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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changeset
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10 </stdio> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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11 <command><![CDATA[ |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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12 Rscript $__tool_directory__/FCS1Dplotggcyto.R '${input}' '${output}' $outformat '${transform.trans_method}' |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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changeset
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13 #if $transform.trans_method == "arcsinh" |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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14 $transform.scaling_factor |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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changeset
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15 #else if $transform.trans_method == "logicle" |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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changeset
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16 $transform.w $transform.t $transform.m |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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17 #end if |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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18 ]]> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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changeset
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19 </command> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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20 <inputs> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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21 <param format="fcs" name="input" type="data" label="FCS file"/> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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22 <conditional name="transform"> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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23 <param name="trans_method" type="select" label="Apply transformation to plot:" help="by default, no transformation"> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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24 <option value="None">no transformation please.</option> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
|
25 <option value="logicle">logicle</option> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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26 <option value="arcsinh">arcsinh</option> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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27 </param> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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28 <when value="arcsinh"> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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29 <param name="scaling_factor" type="integer" min="1" max="200" value="150" label="Scaling factor b for arcsinh transform:" help="The default value is 150 for standard FCM data. The recommended value for cyTOF data is 5."> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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30 </param> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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31 </when> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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32 <when value="logicle"> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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changeset
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33 <param name="w" type="float" value="0.5" label="Linearization width w for logicle transform:" help="w should be positive and determines the slope of transformation at zero. The default value is 0.5."> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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34 </param> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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35 <param name="t" type="integer" value="262144" label="Top of the scale data value t for logicle transform:" help=" t should be greater than zero. Recommended values are 10000 for common 4 decade data or 262144 for a 18 bit data range. The default value is 262144."> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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36 </param> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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changeset
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37 <param name="m" type="float" value="4.5" label="Full width of the transformed display m for logicle transform:" help="m is expressed in asymptotic decades and should be positive. The default value is 4.5 ."> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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38 </param> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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39 </when> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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40 </conditional> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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41 <param name="outformat" type="select" label="Output Format" help="PDF will be larger files that may take some time to load."> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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42 <option value="PNG">PNG</option> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
changeset
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43 <option value="PDF">PDF</option> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
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44 </param> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
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45 </inputs> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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46 <outputs> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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47 <data format="png" name="output" label="1D Density Plots for ${input.name} with ${transform.trans_method} in ${outformat}"> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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48 <change_format> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
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49 <when input="outformat" value="PDF" format="pdf" /> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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50 </change_format> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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51 </data> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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52 </outputs> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
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53 <tests> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
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54 <test> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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55 <param name="input" value="testfcs1.fcs"/> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
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56 <param name="outformat" value="PDF"/> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
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57 <param name="trans_method" value="None"/> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
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58 <output name="output" file="graph.pdf" compare="sim_size"/> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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59 </test> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
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60 <test> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
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61 <param name="input" value="testfcs1.fcs"/> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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62 <param name="outformat" value="PNG"/> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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63 <param name="trans_method" value="logicle"/> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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64 <param name="w" value="0.4"/> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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65 <param name="m" value="4.5"/> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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66 <param name="t" value="10000"/> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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67 <output name="output" file="graph1.png" compare="sim_size"/> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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68 </test> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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69 <test> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
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70 <param name="input" value="testfcs1.fcs"/> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
diff
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71 <param name="outformat" value="PNG"/> |
b73fc4860906
"planemo upload for repository https://github.com/ImmPortDB/immport-galaxy-tools/tree/master/flowtools/ggcyto_1d_density_plots commit b32c86c02e138aa291c869b31351c4970300fbb4"
azomics
parents:
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72 <param name="trans_method" value="arcsinh"/> |
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73 <param name="scaling_factor" value="150"/> |
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74 <output name="output" file="graph2.png" compare="sim_size"/> |
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75 </test> |
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76 </tests> |
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77 <help><![CDATA[ |
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78 Scatterplots of fcs sample |
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79 ------------------- |
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80 |
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81 This tool allows generation of density scatter plots using ggcyto. |
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82 |
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83 **Input files** |
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84 |
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85 This tool takes valid FCS files as input. |
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86 |
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87 **Output files** |
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88 |
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89 This tool generates a series of 1D density plot using ggcyto and produces a png file. A pdf file can optionally be generated. |
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90 |
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91 class:: warningmark |
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92 |
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93 PDF are larger files that may take some time to load. It might be faster to download the PDF output once generated to open it locally. |
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94 |
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95 ----- |
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96 |
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97 **Transformation** |
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98 |
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99 This tools currently supports 2 transformation algorithms: arcsinh and logicle, implemented using flowCore. Transformation applied are color-coded: black is no transformation, blue is arcinsh, and green is logicle. |
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100 |
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101 ----- |
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102 |
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103 **Example** |
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104 |
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105 *Output*: |
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106 |
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107 .. image:: ./static/images/flowtools/testoutputggcyto.png |
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108 |
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109 ----- |
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110 |
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111 **ggcyto reference** |
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112 Jiang M (2015). ggcyto: Visualize Cytometry data with ggplot. R package version 1.4.0, https://github.com/RGLab/ggcyto/issues. |
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113 |
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114 ]]> |
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115 </help> |
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116 <citations> |
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117 <citation type="doi">10.1186/1471-2105-10-106</citation> |
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118 </citations> |
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119 </tool> |