LOCUS       ARBH01000003            3500 bp    DNA     linear   BCT 22-APR-2013
DEFINITION  Amycolatopsis balhimycina FH 1894 strain DSM 44591
            A3CEDRAFT_scaffold1.1_C3, whole genome shotgun sequence.
ACCESSION   ARBH01000003
VERSION     ARBH01000003.1
KEYWORDS    .
SOURCE      Amycolatopsis balhimycina FH 1894
  ORGANISM  Amycolatopsis balhimycina FH 1894
            Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
            Amycolatopsis.
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Amycolatopsis balhimycina FH 1894"
                     /mol_type="genomic DNA"
                     /strain="DSM 44591"
                     /culture_collection="DSM:44591"
                     /db_xref="taxon:1089545"
     cluster         1..3500
                     /note="Cluster number: 1"
                     /note="Detection rule(s) for this cluster type: t1pks:
                     ((PKS_KS & PKS_AT) or (ene_KS & PKS_AT) or (mod_KS &
                     PKS_AT) or (hyb_KS & PKS_AT) or (itr_KS & PKS_AT) or
                     (tra_KS & PKS_AT));"
                     /note="Monomers prediction: (mal)"
                     /note="Structure image: structures/genecluster1.png"
                     /cutoff=20000
                     /extension=20000
                     /product="t1pks"
                     /contig_edge="True"
     CDS             3..3500
                     /aSProdPred="mal"
                     /note="smCOG:
                     SMCOG1001:short-chain_dehydrogenase/reductase_SDR (Score:
                     64.2; E-value: 2.2e-19);"
                     /locus_tag="ctg1_1"
                     /translation="GGVLALPAELDRRGGDRLAAVLSGATGEDQLAIRAAGVFGRRVVR
                     APAGDRAPARTWTPRGTTLITGGTGTLAPHLARWLAEQGAEHIVLTSRTGAEAPKARQL
                     LAELGETVEAVACDVTDKAALAALLARLRAEGRTVRNVVHTAAVIELHTLAETDLAAFS
                     RTVHAKVVGARNLDELLDTDELDAFVLYSSTAGLWGTGAHAAYVAGNAYLHALAAHRRA
                     RGLRATALSWGIWADDRELGRVDPEQIVRSGLVFMAPELALEGLRRALDDDETALAVAD
                     LDWERYYPVYTAVRPTLLFDELPEVRRLTEAAAATAATGAGGEFAARLRTLPEAERAHL
                     LLELVRAEAAAVLGHASADALPEDRAFRDVGFDSVTAVDLRNRISAGTGLTLPATMVFD
                     HPTPRRLAGFLAATITGSGAVEQAPAVAGVDTGEPVAIIGMACRYPGGANTPERLWDLV
                     VGGVDAISGFPADRNWPTDALYDPDPDAGGKTYSVQGGFLHEAAEFDPGFFGISPREAL
                     SMDPQQRLLLETAWEAFERAGIDPHTLRGSGTGTFIGASYQDYTAAVSGAVDNADGHMI
                     TGSLGSILSGRLSYLFGLEGPAVTLDTACSSSLVAIHLAAQSLRSGESSLALAGGVSVM
                     ATPGAFVGFSRQRALATDGRCKAYSDRADGMTLGEGVGLVLLEKLSDAQRNGHRILAVV
                     RGSATNQDGASNGMTAPSGPSQQRVIRQALANARLSASEVDVIEGHGTGTALGDPIEAQ
                     ALLATYGQDRERPLLLGSVKSNIGHTQMASGVAGVIKVVQALRHGLVPKTLHVDEPSTH
                     VDWSTGSIELPSGSVPWPESGRPRRAGISSFGLSGTNVHTILEQAPEPAAEAGPEPEPG
                     LVPVPLSGRTEAALRAQAATVLDTLDDGVSPAVLGYSLASTRSAFEHRAVLLAEDHDEL
                     RRGLAALAGDQPDGGVVRGTVTRGRTAFLFAGQGSQRAGMGRELYERHPVFADALDAVL
                     GHFDLPRALRDVMWDDDSTALDETGYTQPALFAFEVALFRLLESWGVTPDYLAGHSIGE
                     IAAAHVAGVLSLADACALVAARGALMQALPSGGAMVSVRGSEADVAGHLGEDVAVAAVN
                     GPESVVLAGTEDAVLQAAGRLEAAGHKVRRLRVSHAFHSPLMDPVLAEFATVAQGLTYH
                     "
                     /sec_met="Type: t1pks"
                     /sec_met="Domains detected: PP-binding (E-value: 1.3e-15,
                     bitscore: 44.0, seeds: 164); PKS_AT (E-value: 8.7e-82,
                     bitscore: 260.9, seeds: 1682); mod_KS (E-value: 9.4e-224,
                     bitscore: 729.0, seeds: 217); adh_short (E-value: 7.1e-52,
                     bitscore: 162.1, seeds: 230)"
                     /sec_met="Kind: biosynthetic"
                     /sec_met="NRPS/PKS subtype: Type I Modular PKS"
                     /sec_met="NRPS/PKS Domain: PKS_KR (60-237). E-value:
                     1.4e-53. Score: 173.1; Predicted KR activity: active;
                     Predicted KR stereochemistry: A2;"
                     /sec_met="NRPS/PKS Domain: ACP (341-412). E-value: 7.8e-31.
                     Score: 97.8;"
                     /sec_met="NRPS/PKS Domain: PKS_KS (434-858). E-value:
                     3e-179. Score: 587.9;"
                     /sec_met="NRPS/PKS Domain: PKS_AT (958-1166). E-value:
                     4e-80. Score: 260.9; Substrate specificity predictions: mal
                     (PKS signature), mal (Minowa), mal (consensus);"
     aSDomain        183..713
                     /domain="PKS_KR"
                     /locus_tag="ctg1_1"
                     /detection="hmmscan"
                     /database="nrpspksdomains.hmm"
                     /evalue="1.40E-53"
                     /score="173.1"
                     /translation="GTTLITGGTGTLAPHLARWLAEQGAEHIVLTSRTGAEAPKARQLL
                     AELGETVEAVACDVTDKAALAALLARLRAEGRTVRNVVHTAAVIELHTLAETDLAAFSR
                     TVHAKVVGARNLDELLDTDELDAFVLYSSTAGLWGTGAHAAYVAGNAYLHALAAHRRAR
                     GLRATALSWGIWAD"
                     /label="ctg1_1_KR1"
                     /asDomain_id="nrpspksdomains_ctg1_1_KR1"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: A2"
     CDS_motif       192..284
                     /note="NRPS/PKS Motif: PKSI-KR_m1 (e-value: 3.8e-15,
                     bit-score: 48.4)"
                     /locus_tag="ctg1_1"
                     /motif="PKSI-KR_m1"
                     /database="abmotifs"
                     /evalue="3.80E-15"
                     /asDomain_id="nrpspksmotif_ctg1_1_0001"
                     /detection="hmmscan"
                     /score="48.4"
                     /aSTool="pksnrpsmotif"
                     /translation="LITGGTGTLAPHLARWLAEQGAEHIVLTSRT"
                     /label="PKSI-KR_m1"
     CDS_motif       555..665
                     /note="NRPS/PKS Motif: PKSI-KR_m4 (e-value: 1.6e-14,
                     bit-score: 46.4)"
                     /locus_tag="ctg1_1"
                     /motif="PKSI-KR_m4"
                     /database="abmotifs"
                     /evalue="1.60E-14"
                     /asDomain_id="nrpspksmotif_ctg1_1_0002"
                     /detection="hmmscan"
                     /score="46.4"
                     /aSTool="pksnrpsmotif"
                     /translation="LDAFVLYSSTAGLWGTGAHAAYVAGNAYLHALAAHRR"
                     /label="PKSI-KR_m4"
     aSDomain        1026..1238
                     /domain="ACP"
                     /locus_tag="ctg1_1"
                     /detection="hmmscan"
                     /database="nrpspksdomains.hmm"
                     /evalue="7.80E-31"
                     /score="97.8"
                     /translation="LELVRAEAAAVLGHASADALPEDRAFRDVGFDSVTAVDLRNRISA
                     GTGLTLPATMVFDHPTPRRLAGFLAA"
                     /asDomain_id="nrpspksdomains_ctg1_1_Xdom01"
     aSDomain        1305..2576
                     /domain="PKS_KS"
                     /locus_tag="ctg1_1"
                     /detection="hmmscan"
                     /database="nrpspksdomains.hmm"
                     /evalue="3.00E-179"
                     /score="587.9"
                     /translation="VAIIGMACRYPGGANTPERLWDLVVGGVDAISGFPADRNWPTDAL
                     YDPDPDAGGKTYSVQGGFLHEAAEFDPGFFGISPREALSMDPQQRLLLETAWEAFERAG
                     IDPHTLRGSGTGTFIGASYQDYTAAVSGAVDNADGHMITGSLGSILSGRLSYLFGLEGP
                     AVTLDTACSSSLVAIHLAAQSLRSGESSLALAGGVSVMATPGAFVGFSRQRALATDGRC
                     KAYSDRADGMTLGEGVGLVLLEKLSDAQRNGHRILAVVRGSATNQDGASNGMTAPSGPS
                     QQRVIRQALANARLSASEVDVIEGHGTGTALGDPIEAQALLATYGQDRERPLLLGSVKS
                     NIGHTQMASGVAGVIKVVQALRHGLVPKTLHVDEPSTHVDWSTGSIELPSGSVPWPESG
                     RPRRAGISSFGLSGTNVHTILEQAP"
                     /asDomain_id="nrpspksdomains_ctg1_1_Xdom02"
     CDS_motif       1788..1835
                     /note="NRPS/PKS Motif: PKSI-KS_m3 (e-value: 2e-06,
                     bit-score: 19.7)"
                     /locus_tag="ctg1_1"
                     /motif="PKSI-KS_m3"
                     /database="abmotifs"
                     /evalue="2.00E-06"
                     /asDomain_id="nrpspksmotif_ctg1_1_0003"
                     /detection="hmmscan"
                     /score="19.7"
                     /aSTool="pksnrpsmotif"
                     /translation="GPAVTLDTACSSSLVA"
                     /label="PKSI-KS_m3"
     CDS_motif       2211..2255
                     /note="NRPS/PKS Motif: PKSI-KS_m5 (e-value: 0.0019,
                     bit-score: 10.8)"
                     /locus_tag="ctg1_1"
                     /motif="PKSI-KS_m5"
                     /database="abmotifs"
                     /evalue="1.90E-03"
                     /asDomain_id="nrpspksmotif_ctg1_1_0004"
                     /detection="hmmscan"
                     /score="10.8"
                     /aSTool="pksnrpsmotif"
                     /translation="IEGHGTGTALGDPIE"
                     /label="PKSI-KS_m5"
     CDS_motif       2310..2354
                     /note="NRPS/PKS Motif: PKSI-KS_m6 (e-value: 0.0011,
                     bit-score: 11.5)"
                     /locus_tag="ctg1_1"
                     /motif="PKSI-KS_m6"
                     /database="abmotifs"
                     /evalue="1.10E-03"
                     /asDomain_id="nrpspksmotif_ctg1_1_0005"
                     /detection="hmmscan"
                     /score="11.5"
                     /aSTool="pksnrpsmotif"
                     /translation="GSVKSNIGHTQMASG"
                     /label="PKSI-KS_m6"
     CDS_motif       2874..2927
                     /note="NRPS/PKS Motif: PKSI-AT-mM_m1 (e-value: 2.3e-06,
                     bit-score: 19.8)"
                     /locus_tag="ctg1_1"
                     /motif="PKSI-AT-mM_m1"
                     /database="abmotifs"
                     /evalue="2.30E-06"
                     /asDomain_id="nrpspksmotif_ctg1_1_0006"
                     /detection="hmmscan"
                     /score="19.8"
                     /aSTool="pksnrpsmotif"
                     /translation="FLFAGQGSQRAGMGRELY"
                     /label="PKSI-AT-mM_m1"
     aSDomain        2877..3500
                     /domain="PKS_AT"
                     /locus_tag="ctg1_1"
                     /detection="hmmscan"
                     /database="nrpspksdomains.hmm"
                     /evalue="4.00E-80"
                     /score="260.9"
                     /translation="LFAGQGSQRAGMGRELYERHPVFADALDAVLGHFDLPRALRDVMW
                     DDDSTALDETGYTQPALFAFEVALFRLLESWGVTPDYLAGHSIGEIAAAHVAGVLSLAD
                     ACALVAARGALMQALPSGGAMVSVRGSEADVAGHLGEDVAVAAVNGPESVVLAGTEDAV
                     LQAAGRLEAAGHKVRRLRVSHAFHSPLMDPVLAEFATVAQGLTYH"
                     /label="ctg1_1_AT1"
                     /asDomain_id="nrpspksdomains_ctg1_1_AT1"
                     /specificity="PKS signature: mal"
                     /specificity="Minowa: mal"
                     /specificity="consensus: mal"
     CDS_motif       3033..3095
                     /note="NRPS/PKS Motif: PKSI-AT-mM_m2 (e-value: 0.0032,
                     bit-score: 10.9)"
                     /locus_tag="ctg1_1"
                     /motif="PKSI-AT-mM_m2"
                     /database="abmotifs"
                     /evalue="3.20E-03"
                     /asDomain_id="nrpspksmotif_ctg1_1_0007"
                     /detection="hmmscan"
                     /score="10.9"
                     /aSTool="pksnrpsmotif"
                     /translation="DETGYTQPALFAFEVALFRLL"
                     /label="PKSI-AT-mM_m2"
     CDS_motif       3105..3227
                     /note="NRPS/PKS Motif: PKSI-AT-M_m3 (e-value: 2.3e-22,
                     bit-score: 71.3)"
                     /locus_tag="ctg1_1"
                     /motif="PKSI-AT-M_m3"
                     /database="abmotifs"
                     /evalue="2.30E-22"
                     /asDomain_id="nrpspksmotif_ctg1_1_0008"
                     /detection="hmmscan"
                     /score="71.3"
                     /aSTool="pksnrpsmotif"
                     /translation="GVTPDYLAGHSIGEIAAAHVAGVLSLADACALVAARGALMQ"
                     /label="PKSI-AT-M_m3"
     CDS_motif       3312..3350
                     /note="NRPS/PKS Motif: PKSI-AT-M_m5 (e-value: 0.00047,
                     bit-score: 13.4)"
                     /locus_tag="ctg1_1"
                     /motif="PKSI-AT-M_m5"
                     /database="abmotifs"
                     /evalue="4.70E-04"
                     /asDomain_id="nrpspksmotif_ctg1_1_0009"
                     /detection="hmmscan"
                     /score="13.4"
                     /aSTool="pksnrpsmotif"
                     /translation="AAVNGPESVVLAG"
                     /label="PKSI-AT-M_m5"
ORIGIN
        1 ggggcggcgt gctcgccctg cccgccgagc tggaccgccg cggcggcgac cggctggcgg
       61 ctgtgctgtc cggcgccacc ggcgaagacc agctggccat ccgcgccgcc ggcgtgttcg
      121 gccgccgcgt ggtgcgggcc ccggccggcg accgcgcgcc ggcgcggacc tggaccccgc
      181 gcggcaccac gctgatcacc ggcggcaccg gcaccctggc cccgcacctg gcccgctggc
      241 tggccgagca gggcgccgag cacatcgtgc tgaccagccg caccggcgcc gaggccccga
      301 aggcccggca gctgctggcg gagctgggcg agaccgtcga ggcggtggcc tgcgacgtca
      361 ccgacaaggc ggcgctggcc gccctgctgg cgcggttgcg ggccgagggc cggaccgtgc
      421 ggaacgtggt gcacacggcc gccgtgatcg agctgcacac gctggccgag accgacctgg
      481 ccgcgttctc ccggaccgtg cacgccaagg tggtgggcgc gcgcaacctg gacgagctgc
      541 tcgacaccga cgagctcgat gcgttcgtgc tgtactcctc caccgccggc ctgtggggca
      601 ccggcgcgca cgccgcctac gtggccggca acgcgtactt gcacgcgctg gcagcccacc
      661 ggcgcgcccg ggggctgcgg gccaccgcgc tgtcgtgggg catctgggcc gacgaccgcg
      721 aactcggccg ggtcgacccg gagcagatcg tgcgcagcgg cctggtgttc atggcgccgg
      781 agctggcgct ggagggtctg cgccgggccc tggacgacga cgagaccgcg ctggccgtgg
      841 ccgatctgga ctgggagcgg tactacccgg tctacaccgc cgtccggccg acgctgctgt
      901 tcgacgagct gccggaggtg cggcggctca ccgaggccgc cgccgccacg gccgccaccg
      961 gcgccggcgg cgagttcgcc gcccggctgc gcacgctgcc cgaggccgag cgcgcccacc
     1021 tgctcctgga actggtccgg gccgaggccg cggccgtgct gggccacgcg tcggccgacg
     1081 cgctgcccga ggaccgcgcc ttccgcgacg tcggcttcga ctcggtcacc gcggtcgacc
     1141 tgcgcaaccg gatctccgcc ggcaccggcc tgaccctgcc cgccaccatg gtgttcgacc
     1201 acccgacgcc gaggcggctg gccgggttcc tggccgccac gatcaccggc tcgggtgccg
     1261 tcgagcaggc accggccgtg gccggcgtgg acaccggcga gcccgtcgcc atcatcggga
     1321 tggcctgccg ctacccgggt ggcgcgaaca ccccggaacg gttgtgggac ctggtcgtgg
     1381 gcggcgtgga cgccatctcc ggcttcccgg ccgaccgcaa ctggccgacc gacgcgctct
     1441 acgacccgga cccggacgcc ggcggcaaga cctattcggt gcagggcggc ttcctgcacg
     1501 aggcggccga gttcgacccg ggcttcttcg gcatctcgcc gcgggaggca ctgtccatgg
     1561 atccgcagca gcgcctgctg ctggagacgg cgtgggaggc gttcgagcgg gccgggatcg
     1621 acccgcacac gctgcggggc agcggcaccg gcaccttcat cggggccagc taccaggact
     1681 acaccgcggc cgtgtccggc gcggtggaca acgccgacgg ccacatgatc accggctcgc
     1741 tgggcagcat cctgtccggc cggctctcct acctgttcgg gctggagggc ccggcggtca
     1801 ccctggacac cgcctgctcg tcgtcgctgg tcgccatcca cctggccgcg cagtcgctgc
     1861 ggtcggggga gagcagcctg gcgctggccg gcggggtgag cgtgatggcg acgccggggg
     1921 cgttcgtcgg cttctcgcgc cagcgcgcac tggccacgga cggccgttgc aaggcctact
     1981 cggaccgggc cgacggcatg accctcggcg agggcgtcgg cctggtgctg ctggagaagc
     2041 tgtccgacgc gcagcgcaac gggcaccgga tcctggcggt ggtccggggt tcggccacga
     2101 accaggacgg cgcgtccaac ggcatgaccg cgcccagcgg cccgtcccag cagcgggtga
     2161 tccggcaggc gctggccaac gcgcggctct cggcgtccga ggtggacgtg atcgagggcc
     2221 acggcaccgg caccgcgctg ggcgacccga tcgaggccca ggccctgctg gccacctacg
     2281 gccaggaccg ggaacggccg ctgctgctcg gctcggtgaa gtccaacatc ggccacaccc
     2341 agatggcctc cggcgtggcc ggcgtgatca aggtggtgca ggcgctgcgg cacgggctgg
     2401 tacccaagac gctgcacgtg gacgagccct ccacgcacgt cgactggagc accggctcga
     2461 tcgagctgcc gtccggcagc gtgccgtggc cggagagcgg ccggccgcgc cgggccggta
     2521 tctcgtcctt cgggctgagc ggcacgaacg tgcacaccat cctcgagcag gccccggaac
     2581 cggccgccga agccggcccc gagccggagc ccggcctggt gccggtcccg ctgtccggcc
     2641 ggacggaagc agcgctgcgc gctcaggccg ccaccgtgct ggacaccctg gacgacggcg
     2701 tgtcgccggc cgtgctcggg tactcgctgg cctccacccg gtcggccttc gaacaccgtg
     2761 cggtgctgct ggccgaggac cacgacgaac tgcggcgcgg cctggccgca ctggccggcg
     2821 accagccgga cggcggcgtg gtgcggggca ccgtgacgcg gggccgcacg gcgttcctgt
     2881 tcgccggcca gggcagccag cgggccggga tgggccgcga gctgtacgag cgccacccgg
     2941 tgttcgccga cgcgctggac gcggtgctgg ggcacttcga cctgccccgt gcgctgcggg
     3001 acgtgatgtg ggacgacgat tccacggccc tcgacgagac ggggtacacc cagccggcgt
     3061 tgttcgcctt cgaggtggcg ttgttccggt tgctggagtc gtggggtgtg acgccggatt
     3121 acctggccgg gcattcgatc ggtgagatcg ccgcggcgca cgtggccgga gtgttgtcgc
     3181 tggccgatgc ctgtgcgttg gtcgctgcgc ggggtgcgct gatgcaggcg ctgccgtccg
     3241 gcggggccat ggtttcggtg cgcggctccg aggccgacgt cgccgggcac ctcggcgagg
     3301 acgtcgccgt cgcggcggtc aacgggcccg agtcggtggt gctggccggg accgaggacg
     3361 cggtgctcca ggcggccggc cgcctggagg ccgccggcca caaggtccgc cgcctgcggg
     3421 tcagccacgc cttccactcg cccttgatgg atcccgtgct ggccgagttc gcgacggtgg
     3481 ctcagggcct gacctaccac
//