Mercurial > repos > bgruening > bionano_scaffold
diff test-data/configuration.xml @ 0:fec6a0b53189 draft
"planemo upload for repository https://bionanogenomics.com/support/software-downloads/ commit 1d3a1a449c7398c8fad07663224738265f77c0be"
author | bgruening |
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date | Mon, 17 May 2021 15:07:05 +0000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/configuration.xml Mon May 17 15:07:05 2021 +0000 @@ -0,0 +1,293 @@ +<hybridScaffold> + <version> + <flag attr="version" val0="$Id: hybridScaffold_DLE1_config.xml 7702 2018-06-25 20:53:51Z apang $"/> + </version> + <global> + <flag attr="maxmem" val0="960" display="Maximum memory (GB)" group="Global options" description="Define the maximum amount of RAM in gigabytes to be used by each process."/> + <flag attr="maxthreads" val0="54" display="Max threads" group="Global options" description="Define maximum number of threads to be used by each process."/> + <flag attr="maxvirtmem" val0="960"/> + <flag attr="RAmem" val0="3" val1="1"/> + </global> + <fasta2cmap> + <flag attr="enzyme" val0="BspQI" display="Enzyme" group="FASTA to CMAP digestion" description="Define single enzyme for in-silico FASTA to CMAP digestion. Avalible enzymes: BspQI, BbvCI, BsmI, BsrDI, BssSI, DLE1."/> + <flag attr="channelNum" val0="1" display="Channel number" group="FASTA to CMAP digestion" description="Specify the channel the enzyme was used."/> + <flag attr="minLabels" val0="0" display="Minimum label sites" group="FASTA to CMAP digestion" description="Specify minimum number of label sites per digested contig."/> + <flag attr="minLength" val0="0" display="Minimum length (Kb)" group="FASTA to CMAP digestion" description="Specify minimum length in Kb of each digested contig."/> + </fasta2cmap> + <align0> + <flag attr="M" val0="1" val1="3"/> + <flag attr="ScaleDelta" val0="0.02" val1="15"/> + <flag attr="ScaleDeltaBPP"/> + <flag attr="hashScaleDelta" val0="2"/> + <flag attr="res" val0="2.9"/> + <flag attr="FP" val0="0.6"/> + <flag attr="FN" val0="0.06"/> + <flag attr="sf" val0="0.20"/> + <flag attr="sd" val0="0.0"/> + <flag attr="sr" val0="0.01"/> + <flag attr="extend" val0="1"/> + <flag attr="outlier" val0="0.0001"/> + <flag attr="endoutlier" val0="0.001"/> + <flag attr="PVendoutlier"/> + <flag attr="deltaX" val0="12"/> + <flag attr="deltaY" val0="12"/> + <flag attr="xmapchim" val0="12"/> + <flag attr="hashgen" val0="5" val1="7" val2="2.4" val3="1.5" val4="0.05" val5="5.0" val6="1" val7="1" val8="4"/> + <flag attr="hash" val0="-hashdelta" val1="26" val2="10" val3="46"/> + <flag attr="hashMultiMatch" val0="30" val1="10"/> + <flag attr="insertThreads" val0="54"/> + <flag attr="nosplit" val0="2"/> + <flag attr="biaswt" val0="0"/> + <flag attr="T" val0="1e-10" display="P-value" group="Initial alignment" description="Minimum confidence value to output initial alignments. Recommended starting value of 1e-5/genome size in Mb."/> + <flag attr="S" val0="-1000"/> + <flag attr="indel"/> + <flag attr="PVres" val0="2"/> + <flag attr="rres" val0="0.9"/> + <flag attr="MaxSE" val0="0.5"/> + <flag attr="HSDrange" val0="1.0"/> + <flag attr="outlierBC"/> + <flag attr="xmapUnique" val0="12"/> + <flag attr="AlignRes" val0="2."/> + <flag attr="outlierExtend" val0="12" val1="24"/> + <flag attr="Kmax" val0="12"/> + <flag attr="resEstimate"/> + <flag attr="f"/> + <flag attr="mres" val0="0.9"/> + </align0> + <align1> + <flag attr="res" val0="2.9"/> + <flag attr="FP" val0="0.6"/> + <flag attr="FN" val0="0.06"/> + <flag attr="sf" val0="0.20"/> + <flag attr="sd" val0="0.0"/> + <flag attr="sr" val0="0.01"/> + <flag attr="extend" val0="1"/> + <flag attr="outlier" val0="0.0001"/> + <flag attr="endoutlier" val0="0.001"/> + <flag attr="PVendoutlier"/> + <flag attr="deltaX" val0="12"/> + <flag attr="deltaY" val0="12"/> + <flag attr="xmapchim" val0="12"/> + <flag attr="hashgen" val0="5" val1="7" val2="2.4" val3="1.5" val4="0.05" val5="5.0" val6="1" val7="1" val8="4"/> + <flag attr="hash" val0="-hashdelta" val1="26" val2="10" val3="46"/> + <flag attr="hashMultiMatch" val0="30" val1="10"/> + <flag attr="insertThreads" val0="54"/> + <flag attr="nosplit" val0="2"/> + <flag attr="biaswt" val0="0"/> + <flag attr="T" val0="1e-10" display="P-value" group="Initial alignment" description="Minimum confidence value to output initial alignments. Recommended starting value of 1e-5/genome size in Mb."/> + <flag attr="S" val0="-1000"/> + <flag attr="indel"/> + <flag attr="PVres" val0="2"/> + <flag attr="rres" val0="0.9"/> + <flag attr="MaxSE" val0="0.5"/> + <flag attr="HSDrange" val0="1.0"/> + <flag attr="outlierBC"/> + <flag attr="xmapUnique" val0="12"/> + <flag attr="AlignRes" val0="2."/> + <flag attr="outlierExtend" val0="12" val1="24"/> + <flag attr="Kmax" val0="12"/> + <flag attr="resEstimate"/> + <flag attr="f"/> + <flag attr="mres" val0="0.9"/> + </align1> + <assignAlignType> + <flag attr="T_cutoff" val0="1e-11" display="P-value" group="Chimeric/conflicting alignment flagging" description="Minimum confidence value used to flag chimeric/conflicting alignments. Recommand to set it to be the same as the merge_Tvalue below."/> + <flag attr="max_overhang" val0="5" display=""/> + </assignAlignType> + <cut_conflicts> + <flag attr="window_size" val0="10000" display="Distance (bp)" group="Conflict-cut" description="The distance (bp) from a conflicting site within which the chimeric quality score of BioNano genome map labels will be examined"/> + <flag attr="min_quality_score_threshold" val0="35" display="Percent (%)" group="Conflict-cut" description="The minimal percentage of molecules spanning to the left and right of a label of interest, thus supporting the BioNano assembly at that region"/> + <flag attr="min_coverage_threshold" val0="10" display="Coverage (X)" group="Conflict-cut" description="The minimal number of molecules aligning to a label of interest in the BioNano assembly"/> + </cut_conflicts> + <mergeNGS_BN> + <flag attr="merge_Tvalue" val0="1e-11" display="P-value" group="Merging" description="Minimum confidence value used to merge alignments. Recommand to set it to be the same as the assignAlignType T_cutoff above"/> + <flag attr="id_shift" val0="100000" display="BioNano cmap id shift" group="Merging" description="Value to shift the BioNano cmap id to distinguish the BioNano cmaps from sequence cmaps. Recommand to set it to be greater than the number of sequence entries."/> + <flag attr="max_merge_rounds" val0="40" display=""/> + <flag attr="endoutlier" val0="1e-4" display=""/> + <flag attr="outlier" val0="1e-4" display=""/> + <flag attr="biaswt" val0="0" display=""/> + <flag attr="sd" val0="0.1" display=""/> + <flag attr="res" val0="2.9" display=""/> + <flag attr="mres" val0="2.9" display=""/> + <flag attr="sf" val0="0.2" display=""/> + <flag attr="RepeatMask" val0="4" val1="0.01" display=""/> + <flag attr="RepeatRec" val0="0.7" val1="0.6" val2="1.4" display=""/> + <flag attr="pairmerge" val0="80" val1="0.2" display="Min alignment length and Max endoutlier" group="Merging" description="Minimum alignment length required for pair merge, and the maximum endoutlier allowed."/> + <flag attr="maxmem" val0="960" display=""/> + <flag attr="pairmergeRepeat"/> + <flag attr="NoBpp"/> + <flag attr="first" val0="-1" display=""/> + <flag attr="f"/> + </mergeNGS_BN> + <align_final_1st_pass> + <flag attr="res" val0="2.9"/> + <flag attr="FP" val0="0.6"/> + <flag attr="FN" val0="0.06"/> + <flag attr="sf" val0="0.20"/> + <flag attr="sd" val0="0.0"/> + <flag attr="sr" val0="0.01"/> + <flag attr="extend" val0="1"/> + <flag attr="outlier" val0="0.0001"/> + <flag attr="endoutlier" val0="0.001"/> + <flag attr="PVendoutlier"/> + <flag attr="deltaX" val0="12"/> + <flag attr="deltaY" val0="12"/> + <flag attr="xmapchim" val0="12"/> + <flag attr="hashgen" val0="5" val1="7" val2="2.4" val3="1.5" val4="0.05" val5="5.0" val6="1" val7="1" val8="4"/> + <flag attr="hash" val0="-hashdelta" val1="26" val2="10" val3="46"/> + <flag attr="hashMultiMatch" val0="30" val1="10"/> + <flag attr="insertThreads" val0="54"/> + <flag attr="nosplit" val0="2"/> + <flag attr="biaswt" val0="0"/> + <flag attr="T" val0="1e-10" display="P-value" group="Initial alignment" description="Minimum confidence value to output intial alignments. Recommended starting value of 1e-5/genome size in Mb."/> + <flag attr="S" val0="-1000"/> + <flag attr="indel"/> + <flag attr="PVres" val0="2"/> + <flag attr="rres" val0="0.9"/> + <flag attr="MaxSE" val0="0.5"/> + <flag attr="HSDrange" val0="1.0"/> + <flag attr="outlierBC"/> + <flag attr="xmapUnique" val0="12"/> + <flag attr="AlignRes" val0="2."/> + <flag attr="outlierExtend" val0="6" val1="24"/> + <flag attr="Kmax" val0="6"/> + <flag attr="resEstimate"/> + <flag attr="f"/> + <flag attr="mres" val0="0.9"/> + <flag attr="MultiMatches" val0="5"/> + </align_final_1st_pass> + <align_final_2nd_pass> + <flag attr="res" val0="2.9"/> + <flag attr="FP" val0="0.6"/> + <flag attr="FN" val0="0.06"/> + <flag attr="sf" val0="0.20"/> + <flag attr="sd" val0="0.0"/> + <flag attr="sr" val0="0.01"/> + <flag attr="extend" val0="1"/> + <flag attr="outlier" val0="0.0001"/> + <flag attr="endoutlier" val0="0.001"/> + <flag attr="PVendoutlier"/> + <flag attr="deltaX" val0="12"/> + <flag attr="deltaY" val0="12"/> + <flag attr="xmapchim" val0="12"/> + <flag attr="hashgen" val0="5" val1="3" val2="2.4" val3="1.5" val4="0.05" val5="5.0" val6="1" val7="1" val8="4"/> + <flag attr="hash" val0="-hashdelta" val1="50"/> + <flag attr="hashMultiMatch" val0="30" val1="10" val2="3"/> + <flag attr="insertThreads" val0="54"/> + <flag attr="nosplit" val0="2"/> + <flag attr="biaswt" val0="0"/> + <flag attr="T" val0="1e-10" display="P-value" group="Final alignment" description="Minimum confidence score used to align NGS contigs back to hybrid scaffold."/> + <flag attr="S" val0="-1000"/> + <flag attr="indel"/> + <flag attr="PVres" val0="2"/> + <flag attr="rres" val0="0.9"/> + <flag attr="MaxSE" val0="0.5"/> + <flag attr="HSDrange" val0="1.0"/> + <flag attr="outlierBC"/> + <flag attr="xmapUnique" val0="12"/> + <flag attr="AlignRes" val0="2."/> + <flag attr="outlierExtend" val0="12" val1="24"/> + <flag attr="Kmax" val0="12"/> + <flag attr="resEstimate"/> + <flag attr="f"/> + <flag attr="mres" val0="0.9"/> + <flag attr="MultiMatches" val0="5"/> + </align_final_2nd_pass> + <align_final_BNG> + <flag attr="res" val0="2.9"/> + <flag attr="FP" val0="0.6"/> + <flag attr="FN" val0="0.06"/> + <flag attr="sf" val0="0.20"/> + <flag attr="sd" val0="0.0"/> + <flag attr="sr" val0="0.01"/> + <flag attr="extend" val0="1"/> + <flag attr="outlier" val0="0.0001"/> + <flag attr="endoutlier" val0="0.001"/> + <flag attr="PVendoutlier"/> + <flag attr="deltaX" val0="6"/> + <flag attr="deltaY" val0="6"/> + <flag attr="xmapchim" val0="12"/> + <flag attr="hashgen" val0="5" val1="7" val2="2.4" val3="1.5" val4="0.05" val5="5.0" val6="1" val7="1" val8="4"/> + <flag attr="hash" val0="-hashdelta" val1="26" val2="10" val3="46"/> + <flag attr="hashMultiMatch" val0="30" val1="10"/> + <flag attr="insertThreads" val0="4"/> + <flag attr="nosplit" val0="2"/> + <flag attr="biaswt" val0="0"/> + <flag attr="T" val0="1e-10" display="P-value" group="Final alignment" description="Minimum confidence score used to align NGS contigs back to hybrid scaffold."/> + <flag attr="S" val0="-1000"/> + <flag attr="indel"/> + <flag attr="PVres" val0="2"/> + <flag attr="rres" val0="0.9"/> + <flag attr="MaxSE" val0="0.5"/> + <flag attr="HSDrange" val0="1.0"/> + <flag attr="outlierBC"/> + <flag attr="xmapUnique" val0="14"/> + <flag attr="AlignRes" val0="2."/> + <flag attr="outlierExtend" val0="6" val1="24"/> + <flag attr="Kmax" val0="6"/> + <flag attr="resEstimate"/> + <flag attr="BestRef" val0="1"/> + <flag attr="f"/> + <flag attr="mres" val0="0.9"/> + </align_final_BNG> + <refineFinal1> + <flag attr="refine" val0="1" display="Refine Map" group="Final Refinement" default0="3"/> + <flag attr="usecolor" val0="1" default0="1" display="Color Channel" group="Final Refinement"/> + <flag attr="A" val0="5" display="Aligned Sites Threshold" group="Final Refinement" default0="5"/> + <flat attr="S" val0="-9"/> + <flag attr="L" val0="130"/> + <flag attr="maptype" val0="0"/> + <flag attr="extend" val0="1" display="Allow Extended Molecule" group="Final Refinement" default0="1"/> + <flag attr="MaxCov" val0="100" display="Max Coverage" group="Final Refinement" default0="100"/> + <flag attr="Mprobeval" display="Fast Mode" group="Final Refinement"/> + <flag attr="splitcnt"/> + <flag attr="splitrev" val0="2"/> + <flag attr="CmapSNR"/> + <flag attr="splitsite"/> + <flag attr="MinSplitLen" val0="50.0"/> + <flag attr="MaxSE" val0="0.5"/> + <flag attr="outlier" val0="2e-2" display="Min Outliers P-value" group="Second Refinement" default0="1e-5"/> + <flag attr="outlierMax" val0="80."/> + <flag attr="outlierLambda" val0="20."/> + <flag attr="endoutlier" val0="1e-3" display="Molecule Ends P-value Cutoff" group="Second Refinement" default0="1e-4"/> + <flag attr="endoutlierRef" val0="1e-4" val1="1e-3"/> + <flag attr="skip_dist" val0="0.0" val2="0.0"/> + <flag attr="endoutlierFinal" val0="1"/> + <flag attr="maxEnd" val0="90."/> + <flag attr="RTHETA_FIX" val0="0"/> + <flag attr="TB" val0="1e-6" val1="1e-6" val2="-TBmult" val3="0.1"/> + <flag attr="nosplit" val0="2" display="Allow Chimeric Split" group="Final Refinement" default0="2"/> + <flag attr="EndTrim" val0="0.0" display="Min End Trim Coverage" group="Final Refinement" default0="4.99"/> + <flag attr="biaswt" val0="0.7" display="Bias" group="Final Refinement" default0="0"/> + <flag attr="biaswtEnd" val0="0.0"/> + <flag attr="biaswtRefine" val0="0.7" val2="1"/> + <flag attr="biaswtOutlier" val0="0.0"/> + <flag attr="LRbias" val0="1e0" display="Soft Threshold" group="Final Refinement" default0="1e2"/> + <flag attr="deltaX" val0="6" display="Molecule Labels Metric" group="Final Refinement" default0="4"/> + <flag attr="deltaY" val0="7" display="Mapped Labels Metric" group="Final Refinement" default0="6"/> + <flag attr="RepeatMask" val0="5" val1="0.01" display="Repeat Mask P-values" group="Final Refinement" default0="2" default1="0.01"/> + <flag attr="RepeatRec" val0="0.7" val1="0.6" val2="1.4"/> + <flag attr="CovTrim" val0="2"/> + <flag attr="CovTrimLen" val0="55"/> + <flag attr="TrimNorm" val0="0"/> + <flag attr="TrimNormMed" val0="100"/> + <flag attr="TrimNormChim" val0="2"/> + <flag attr="TrimNormMin" val0="2"/> + <flag attr="PVres" val0="2"/> + <flag attr="PVendoutlier"/> + <flag attr="AlignRes" val0="1.5"/> + <flag attr="rres" val0="1.2"/> + <flag attr="cres" val0="5.6" val1="3" val2="0.1"/> + <flag attr="hashgen" val00="5" val01="3" val02="2.4" val03="1.5" val04="0.05" val05="5.0" val06="1" val07="1" val08="3"/> + <flag attr="hash" val00="-hashdelta" val01="10" val02="10" val03="-mres" val04="0.9"/> + <flag attr="HSDrange" val0="1.0"/> + <flag attr="hashoffset" val0="1"/> + <flag attr="hashMultiMatch" val0="15"/> + <flag attr="insertThreads" val0="54"/> + <flag attr="BestRef" val0="1"/> + <flag attr="BestRefPV" val0="1"/> + <flag attr="ChimQuality"/> + <flag attr="f" display="Overwrite Output Files" group="Final Refinement"/> + <flag attr="T" val0="1e-11" display="P Value Cutoff Threshold"/> + </refineFinal1> +</hybridScaffold>