Mercurial > repos > bgruening > bismark
view bismark_deduplicate_wrapper.py @ 15:0b656f8c5637 draft
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/bismark commit ec1f38df34e6862abd0b8e7cc0521e25f9933567
author | bgruening |
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date | Thu, 01 Aug 2019 10:47:13 -0400 |
parents | 9bfe38410155 |
children | 120b7b35e442 |
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#!/usr/bin/python import argparse import logging import os import shutil import subprocess import sys import signal import tempfile from glob import glob def stop_err(logger, msg): logger.critical(msg) sys.exit(1) def restore_sigpipe(): """ Needed to handle samtools view """ signal.signal(signal.SIGPIPE, signal.SIG_DFL) def log_subprocess_output(logger, pipe): for line in iter(pipe.readline, b''): logger.debug(line.decode().rstrip()) def get_arg(): parser = argparse.ArgumentParser() parser.add_argument('--single_or_paired', dest='single_or_paired') parser.add_argument('--input', dest='input', metavar='input') parser.add_argument('--output_report', dest='output_report', metavar='output_report') parser.add_argument('--output_bam', dest='output_bam', metavar='output_report') parser.add_argument('--log_report', dest='log_report', metavar='log_filename', type=str) args = parser.parse_args() return args def __main__(): args = get_arg() logger = logging.getLogger('bismark_deduplicate_wrapper') logger.setLevel(logging.DEBUG) ch = logging.StreamHandler(sys.stdout) if args.log_report: ch.setLevel(logging.WARNING) handler = logging.FileHandler(args.log_report) handler.setLevel(logging.DEBUG) logger.addHandler(handler) else: ch.setLevel(logging.DEBUG) logger.addHandler(ch) # ensure the input has a .bam suffix tmp_dir = tempfile.mkdtemp(prefix='tmp', suffix='') os.chdir(tmp_dir) default_reads_name = 'submitted_reads.bam' os.symlink(args.input, default_reads_name) single_or_paired = '-s' if args.single_or_paired == 'single' else '-p' cmd = ['deduplicate_bismark', single_or_paired, default_reads_name, '--bam'] logger.info("Deduplicating with: '%s'", " ".join(cmd)) process = subprocess.Popen(cmd, stdout=subprocess.PIPE, stderr=subprocess.STDOUT, preexec_fn=restore_sigpipe) proc_out, proc_err = process.communicate() logger.info(proc_out) if process.returncode != 0: stop_err(logger, "Bismark deduplication error (also check the log file if any)!\n%s" % proc_err) deduplicated_out_name = 'submitted_reads.deduplicated.bam' deduplicated_report_name = 'submitted_reads.deduplication_report.txt' logger.debug("Moving '%s' to galaxy: '%s'.", deduplicated_out_name, args.output_bam) shutil.move(deduplicated_out_name, args.output_bam ) logger.debug("Moving '%s' to galaxy: '%s'.", deduplicated_report_name, args.output_report) shutil.move('submitted_reads.deduplication_report.txt', args.output_report) logger.debug("Done.") if __name__=="__main__": __main__()