Mercurial > repos > bgruening > blobtoolkit
annotate blobtoolkit.xml @ 2:53dd00a4ab68 draft
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 5078c7010ec46fd01fec2057dd4486781c958c37
author | bgruening |
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date | Tue, 28 Mar 2023 15:17:19 +0000 |
parents | 840822c1e84a |
children | 8c2167481e72 |
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7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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1 <tool id="blobtoolkit" name="BlobToolKit" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="21.05"> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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2 <description>genome assembly QC</description> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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3 <macros> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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4 <import>macros.xml</import> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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5 </macros> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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6 <expand macro='requirements' /> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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7 <command detect_errors="exit_code"><![CDATA[ |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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8 #if $mode_conditional.selector == 'create' |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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9 mkdir -p './Blobdir' && |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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10 mkdir -p './taxdump' && |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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11 ln -s '${mode_conditional.taxdump}' 'taxdump.tar' && |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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12 tar -xf 'taxdump.tar' -C './taxdump' && |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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13 ln -s '${mode_conditional.fasta}' 'assembly.fasta' && |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
bgruening
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14 #if $mode_conditional.meta |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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15 ln -s '${mode_conditional.meta}' 'assembly.yaml' && |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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16 #end if |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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17 blobtools create |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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18 --fasta 'assembly.fasta' |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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19 #if $mode_conditional.meta |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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20 --meta 'assembly.yaml' |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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21 #end if |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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22 --taxid $mode_conditional.taxid |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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23 --taxdump './taxdump' |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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24 './Blobdir' |
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53dd00a4ab68
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 5078c7010ec46fd01fec2057dd4486781c958c37
bgruening
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25 && tar -C ./Blobdir -zcvf Blobdir.tgz . |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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26 #else if $mode_conditional.selector == 'add' |
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53dd00a4ab68
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 5078c7010ec46fd01fec2057dd4486781c958c37
bgruening
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27 mkdir -p './Blobdir' && |
53dd00a4ab68
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 5078c7010ec46fd01fec2057dd4486781c958c37
bgruening
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28 mkdir -p './taxdump' && |
53dd00a4ab68
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 5078c7010ec46fd01fec2057dd4486781c958c37
bgruening
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29 ln -s '${mode_conditional.taxdump}' 'taxdump.tar' && |
53dd00a4ab68
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 5078c7010ec46fd01fec2057dd4486781c958c37
bgruening
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30 tar -xf 'taxdump.tar' -C './taxdump' && |
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7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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31 #if $mode_conditional.blobdir.is_of_type("tgz") |
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53dd00a4ab68
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 5078c7010ec46fd01fec2057dd4486781c958c37
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32 tar -zxf '${mode_conditional.blobdir}' -C './Blobdir' && |
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7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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33 #else |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 5078c7010ec46fd01fec2057dd4486781c958c37
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34 tar -xf '${mode_conditional.blobdir}' -C './Blobdir' && |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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35 #end if |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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36 #if $mode_conditional.busco |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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37 ln -s '${mode_conditional.busco}' './busco_results.tab' && |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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38 #end if |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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39 #if $mode_conditional.blast_input.selector == 'enabled' |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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40 ln -s '$mode_conditional.blast_input.hits' './blast_results.tab' && |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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41 #end if |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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42 #if $mode_conditional.cov |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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43 ln -s '$mode_conditional.cov' './input.bam' && |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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44 #end if |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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45 #if $mode_conditional.bed |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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46 mkdir -p './bed_files' && |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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47 #import re |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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48 #for $i, $input in enumerate($mode_conditional.bed): |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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49 #set $safename = re.sub('[^\w\-_]', '_', $input.element_identifier) + "." + str($i) |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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50 ln -sf '${input}' './bed_files/${safename}.bed' && |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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51 #end for |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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52 #end if |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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53 blobtools add |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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54 --threads \${GALAXY_SLOTS:-8} |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 5078c7010ec46fd01fec2057dd4486781c958c37
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55 --taxdump './taxdump' |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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56 #if $mode_conditional.busco |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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57 --busco './busco_results.tab' |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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58 #end if |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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59 #if $mode_conditional.blast_input.selector == 'enabled' |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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60 --hits './blast_results.tab' |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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61 #if $mode_conditional.blast_input.hits_cols |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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62 --hits-cols '${$mode_conditional.blast_input.hits_cols}' |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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63 #end if |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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64 --taxrule $mode_conditional.blast_input.taxrule |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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65 --evalue $mode_conditional.blast_input.evalue |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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66 --hit-count $mode_conditional.blast_input.hit_count |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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67 --bitscore $mode_conditional.blast_input.bitscore |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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68 #end if |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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69 #if $mode_conditional.bed |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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70 --beddir './bed_files' |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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71 #end if |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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72 #if $mode_conditional.cov |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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73 --cov './input.bam' |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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74 #end if |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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75 #if $mode_conditional.fasta |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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76 --fasta '${mode_conditional.fasta}' |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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77 #end if |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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78 #if $mode_conditional.trnascan |
7d9ee8cd14b8
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/blobtoolkit commit 7c0db48ffd4dbee68fb2320fc5a064bc9c3219d9
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79 --trnascan '${mode_conditional.trnascan}' |
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80 #end if |
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81 #if $mode_conditional.text_input.selector == 'enabled' |
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82 --text '${mode_conditional.text_input.text}' |
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83 --text-cols '${mode_conditional.text_input.text_cols}' |
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84 $mode_conditional.text_input.text_header |
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85 $mode_conditional.text_input.text_no_array |
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86 --text-delimiter $mode_conditional.text_input.text_delimiter |
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87 #end if |
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88 #if $mode_conditional.advanced_options.blobdb |
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89 --blobdb '${mode_conditional.advanced_options.blobdb}' |
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90 #end if |
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91 #if $mode_conditional.advanced_options.synonyms |
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92 --synonyms '${mode_conditional.advanced_options.synonyms}' |
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93 #end if |
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94 $mode_conditional.advanced_options.update_plot |
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95 #if $mode_conditional.advanced_options.pileup_args |
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96 --pileup-args '${mode_conditional.advanced_options.pileup_args}' |
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97 #end if |
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98 $mode_conditional.advanced_options.replace |
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99 './Blobdir' |
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100 && tar -C ./Blobdir -zcvf Blobdir.tgz . |
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101 |
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102 #else if $mode_conditional.selector == 'filter' |
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103 mkdir -p './Blobdir' && |
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104 #if $mode_conditional.blobdir.is_of_type("tgz") |
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105 tar -zxf '${mode_conditional.blobdir}' -C './Blobdir' && |
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106 #else |
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107 tar -xf '${mode_conditional.blobdir}' -C './Blobdir' && |
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108 #end if |
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109 #if $mode_conditional.filter_options.list |
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110 echo $mode_conditional.filter_options.list > id_list.txt && |
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111 #end if |
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112 #if $mode_conditional.blobdir.is_of_type("tgz") |
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113 tar -zxf '${mode_conditional.blobdir}' -C './' && |
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114 #else |
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115 tar -xf '${mode_conditional.blobdir}' -C './' && |
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116 #end if |
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117 #if $mode_conditional.filter_options.fasta |
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118 ln -s '${mode_conditional.filter_options.fasta}' './input.fasta' && |
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119 #end if |
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120 #if $mode_conditional.filter_options.fastq_filter.selector == 'enabled' |
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121 ln -s '${mode_conditional.filter_options.fastq_filter.fastq}' './input.fastq' && |
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122 ln -s '$mode_conditional.filter_options.fastq_filter.cov' './input.bam' && |
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123 #end if |
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124 blobtools filter |
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125 #if $mode_conditional.filter_options.param |
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126 --param $mode_conditional.filter_options.param |
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127 #end if |
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128 #if $mode_conditional.filter_options.json |
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129 --json $mode_conditional.filter_options.json |
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130 #end if |
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131 #if $mode_conditional.filter_options.list |
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132 --list id_list.txt |
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133 #end if |
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134 $mode_conditional.filter_options.invert |
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135 #if $mode_conditional.filter_options.fasta |
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136 --fasta './input.fasta' |
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137 #end if |
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138 #if $mode_conditional.filter_options.fastq_filter.selector == 'enabled' |
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139 --fastq './input.fastq' |
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140 --cov './input.bam' |
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141 #end if |
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142 #if $mode_conditional.filter_options.text_input.selector == 'enabled' |
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143 --text '${mode_conditional.filter_options.text_input.text}' |
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144 --text-cols '${mode_conditional.filter_options.text_input.text_cols}' |
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145 $mode_conditional.filter_options.text_input.text_header |
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146 --text-delimiter $mode_conditional.filter_options.text_input.text_delimiter |
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147 #end if |
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148 ./Blobdir |
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149 #if $mode_conditional.remove_options.remove_selector or $mode_conditional.remove_options.field |
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150 && blobtools remove |
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151 #for $param in $mode_conditional.remove_options.remove_selector |
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152 $param |
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153 #end for |
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154 #if $mode_conditional.remove_options.field |
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155 --field $mode_conditional.remove_options.field |
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156 #end if |
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157 ./Blobdir |
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158 #end if |
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159 && tar -C ./Blobdir -zcvf Blobdir.tgz . |
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160 #end if |
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161 ]]></command> |
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162 <inputs> |
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163 <conditional name="mode_conditional"> |
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164 <param name="selector" type="select" label="Select mode" help="Select a BlobToolKit module"> |
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165 <option value="create">Create a BlobToolKit dataset</option> |
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166 <option value="add">Add data to a BlobToolKit dataset</option> |
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167 <option value="filter">Edit a BlobToolKit dataset</option> |
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168 </param> |
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169 <when value="create"> |
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170 <param argument="--fasta" type="data" format="fasta" label="Genome assembly file" help="FASTA sequence file" /> |
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171 <param argument="--meta" type="data" format="yaml" label="Metadata file" optional="true" help="Optional metadata dataset"/> |
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172 <param argument="--taxid" type="integer" value="" label="NCBI taxonomy ID" help="Add ranks to metadata for a taxid"/> |
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173 <expand macro="macro_taxdump"/> |
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174 </when> |
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175 <when value="add"> |
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176 <param name="blobdir" type="data" format="tgz" label="Blobdir.tgz file" help="This file should be generated by Blobtool create" /> |
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177 <expand macro="macro_taxdump"/> |
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178 <param argument="--busco" type="data" format="tsv,tabular,txt" optional="true" label="BUSCO full table file"/> |
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179 <conditional name="blast_input"> |
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180 <param name="selector" type="select" label="BLAST/Diamond hits"> |
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181 <option value="enabled">Enabled</option> |
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182 <option value="disabled" selected="true">Disabled</option> |
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183 </param> |
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184 <when value="enabled"> |
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185 <param argument="--hits" type="data" format="tsv,tabular" optional="true" label="BLAST/Diamond hits dataset" help="Tabular BLAST/Diamond output file"/> |
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186 <param argument="--taxrule" type="select" label="BLAST hits to taxa rule" help="Rule to use when assigning BLAST hits to taxa"> |
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187 <option value="bestsum">Bestsum</option> |
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188 <option value="bestsumorder">Bestsumorder</option> |
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189 <option value="bestdistsum">Bestdistsum</option> |
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190 <option value="bestdistsumorder">Bestdistsumorder</option> |
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191 <option value="blastp">Blastp</option> |
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192 </param> |
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193 <param argument="--evalue" type="float" min="0" value="1" label="E-value cutoff" |
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194 help="The smaller the E-value, the better the match. Any hits with an evalue weaker than the value specified will be excluded"/> |
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195 <param argument="--bitscore" type="float" min="0" value="1" label="Bitscore cutoff" |
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196 help="The higher the bit-score, the better the sequence similarity. Any hits with an bitscore lower the value specified will be excluded"/> |
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197 <param argument="--hit-count" type="integer" min="1" value="10" label="Hits to a given taxon" optional="true" |
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198 help="By default the 10 highest scoring hits to a given taxon will be used when applying the --taxrule"/> |
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199 <param argument="--hits-cols" type="text" value="1=qseqid,2=staxids,3=bitscore,5=sseqid,10=qstart,11=qend,14=evalue" optional="true" |
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200 label="BLAST/Diamond file column order" help="More information in the help section"> |
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201 <sanitizer invalid_char=""> |
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202 <valid initial="string.letters,string.digits"> |
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203 <add value="=" /> |
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204 <add value="," /> |
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205 </valid> |
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206 </sanitizer> |
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207 <validator type="regex">[0-9a-z=,]+</validator> |
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208 </param> |
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209 </when> |
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210 <when value="disabled"/> |
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211 </conditional> |
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212 <param argument="--bed" type="data" format="bed" multiple="true" optional="true" label="BED file of coverages per scaffold"/> |
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213 <param argument="--cov" type="data" format="bam,sam,cram" optional="true" label="BAM/SAM/CRAM read alignment file"/> |
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214 <param argument="--fasta" type="data" format="fasta" optional="true" label="FASTA sequence" help="FASTA sequence file" /> |
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215 <param argument="--trnascan" type="data" format="tsv,tabular" optional="true" label="tRNAscan2-SE" help="tRNAscan-SE is employed for identifying and annotating tRNA genes in genomes"/> |
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216 <expand macro="macro_text_input"> |
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217 <param argument="--text-no-array" type="boolean" truevalue="--text-no-array" falsevalue="" checked="false" label="Prevent duplicated identifiers" |
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218 help="Prevent fields in files with duplicate identifiers being loaded as array fields" /> |
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219 </expand> |
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220 <section name="advanced_options" title="Advanced options"> |
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221 <param argument="--blobdb" type="data" format="json" optional="true" label="Blobtools v1 database" help="This file should have been generated with the previous Blobtools tool version"/> |
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222 <param argument="--synonyms" type="data" format="tsv" optional="true" label="Identifier and sinonyms" help="TSV file containing current identifiers and synonyms"/> |
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223 <param argument="--update-plot" type="boolean" truevalue="--update-plot" falsevalue="" checked="false" label="Update plot" help="Flag to use new taxrule as default category" /> |
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224 <param argument="--pileup-args" type="text" value="" optional="true" label="Samtools Pileup" help="Key/value pairs to pass to samtools pileup"> |
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225 <sanitizer invalid_char=""> |
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226 <valid initial="string.letters,string.digits"> |
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227 <add value="=" /> |
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228 <add value="," /> |
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229 <add value="-" /> |
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230 </valid> |
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231 </sanitizer> |
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232 <validator type="regex">[0-9a-zA-Z=,-]+</validator> |
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233 </param> |
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234 <param argument="--replace" type="boolean" truevalue="--replace" falsevalue="" checked="false" label="Replace fields" help="Allow existing fields to be overwritten" /> |
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235 </section> |
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236 </when> |
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237 <when value="filter"> |
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238 <param name="blobdir" type="data" format="tgz" label="Blobdir file" help="This file should be generated by the moudule create" /> |
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239 <section name="filter_options" title="Filter mode options" expanded="true"> |
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240 <param argument="--param" type="text" value="" optional="true" label="Parameter value" help="String of type param=value. Individual param=value pairs can be specified to |
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241 filter based on Variable or Category fields"> |
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242 <sanitizer invalid_char=""> |
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243 <valid initial="string.letters,string.digits"> |
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244 <add value="=" /> |
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245 <add value="," /> |
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246 <add value="-" /> |
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247 </valid> |
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248 </sanitizer> |
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249 <validator type="regex">[0-9a-zA-Z=,-]+</validator> |
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250 </param> |
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251 <param argument="--list" type="text" value="" optional="true" label="List of identifiers" help="Space separated list of identifiers"> |
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252 <sanitizer invalid_char=""> |
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253 <valid initial="string.letters,string.digits"> |
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254 <add value=" " /> |
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255 <add value="-" /> |
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256 <add value="_" /> |
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257 </valid> |
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258 </sanitizer> |
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259 <validator type="regex">[0-9a-zA-Z _-]+</validator> |
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260 </param> |
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261 <param argument="--json" type="data" format="json" optional="true" label="JSON format list file" help="JSON format list file as generated by BlobtoolKit Viewer"/> |
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262 <param argument="--fasta" type="data" format="fasta" multiple="true" optional="true" label="Assembly to be filtered" help="FASTA format assembly file to be filtered"/> |
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263 <conditional name="fastq_filter"> |
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264 <param name="selector" type="select" label="Filter FASTQ reads"> |
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265 <option value="disabled">Disabled</option> |
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266 <option value="enabled">Enabled</option> |
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267 </param> |
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268 <when value="disabled"/> |
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269 <when value="enabled"> |
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270 <param argument="--fastq" type="data" format="fastq" optional="true" label="Reads to be filtered" help="FASTQ format read file to be filtered"/> |
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271 <param argument="--cov" type="data" format="bam,sam,cram" optional="true" label="BAM/SAM/CRAM read alignment file" /> |
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272 </when> |
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273 </conditional> |
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274 <expand macro="macro_text_input"/> |
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275 <param argument="--summary-rank" type="text" value="phylum" label="Sumamry rank" help="Taxonomic level for summary"> |
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276 <sanitizer invalid_char=""> |
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277 <valid initial="string.letters"/> |
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278 </sanitizer> |
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279 <validator type="regex">[a-zA-Z]+</validator> |
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280 </param> |
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281 <param argument="--invert" type="boolean" truevalue="--invert" falsevalue="" checked="false" label="Invert filter" help="Invert the filter (exclude matching records)"/> |
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282 </section> |
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283 <section name="remove_options" title="Remove mode options" expanded="true"> |
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284 <param name="remove_selector" type="select" multiple="true" display="checkboxes" label="Fields to remove"> |
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285 <option value="--all">Remove all fields except identifiers (--all)</option> |
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286 <option value="--busco">Remove all BUSCO fields (--busco)</option> |
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287 <option value="--cov">Remove all cov and read_cov fields (--cov)</option> |
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288 <option value="--fasta">Remove gc, length and ncount fields (--fasta)</option> |
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289 <option value="--hits">Remove all taxonomy fields (--hits)</option> |
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290 </param> |
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291 <param argument="--field" type="text" value="" optional="true" label="Remove fields by ID"> |
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292 <sanitizer invalid_char=""> |
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293 <valid initial="string.letters,string.digits"/> |
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294 </sanitizer> |
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295 <validator type="regex">[0-9a-zA-Z]+</validator> |
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296 </param> |
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297 </section> |
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298 </when> |
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299 </conditional> |
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300 </inputs> |
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301 <outputs> |
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302 <data name="blobdir" format="tgz" from_work_dir="./Blobdir.tgz" label="${tool.name} on ${on_string}: Blobdir.tgz"> |
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303 <filter>mode_conditional['selector'] == 'create' or mode_conditional['selector'] == 'add' or mode_conditional['selector'] == 'filter'</filter> |
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304 </data> |
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305 </outputs> |
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306 <tests> |
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307 <test expect_num_outputs="1"> |
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308 <conditional name="mode_conditional"> |
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309 <param name="selector" value="create"/> |
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310 <param name="fasta" value="assembly.fasta.gz"/> |
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311 <param name="meta" value="assembly.yaml"/> |
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312 <param name="taxid" value="801"/> |
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313 <param name="taxdump" value="small_taxdump.tar.gz"/> |
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314 </conditional> |
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315 <output name="blobdir" file="test01_blobdir.tar.gz" compare="sim_size" delta="100"/> |
0
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316 </test> |
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317 <test expect_num_outputs="1"> |
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318 <conditional name="mode_conditional"> |
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319 <param name="selector" value="add"/> |
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320 <param name="blobdir" value="test01_blobdir.tar.gz"/> |
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321 <param name="taxdump" value="small_taxdump.tar.gz"/> |
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322 <param name="busco" value="busco_full_table.tabular"/> |
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323 <param name="cov" value="bam_file.bam"/> |
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324 <param name="fasta" value="contig.fasta"/> |
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325 <section name="advanced_options"> |
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326 <param name="update_plot" value="false"/> |
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327 </section> |
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328 <conditional name="blast_input"> |
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329 <param name="selector" value="enabled"/> |
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330 <param name="hits" value="blast_results.tabular"/> |
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331 <param name="taxrule" value="bestsum"/> |
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332 <param name="evalue" value="1"/> |
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333 <param name="bitscore" value="1"/> |
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334 <param name="hit_count" value="10"/> |
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335 <param name="hits_cols" value="1=qseqid,2=staxids,3=bitscore,5=sseqid,10=qstart,11=qend,14=evalue"/> |
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336 </conditional> |
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337 </conditional> |
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338 <output name="blobdir" file="test02_blobdir.tar.gz" compare="sim_size" delta="100"/> |
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339 </test> |
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340 <test expect_num_outputs="1"> |
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341 <conditional name="mode_conditional"> |
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342 <param name="selector" value="filter"/> |
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343 <param name="blobdir" value="test02_blobdir.tar.gz"/> |
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344 <section name="filter_options"> |
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345 <param name="fasta" value="contig.fasta"/> |
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346 <param name="summary_rank" value="phylum"/> |
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347 <param name="invert" value="false"/> |
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348 </section> |
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349 <section name="remove_options"> |
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350 <param name="remove_selector" value="--all"/> |
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351 </section> |
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352 </conditional> |
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353 <output name="blobdir"> |
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354 <assert_contents> |
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355 <has_size value="620" delta="50"/> |
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356 </assert_contents> |
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357 </output> |
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358 </test> |
1
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359 <test expect_num_outputs="1"> |
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360 <conditional name="mode_conditional"> |
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361 <param name="selector" value="filter"/> |
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362 <param name="blobdir" value="test02_blobdir.tar.gz"/> |
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363 <section name="filter_options"> |
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364 <param name="list" value="801 802"/> |
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365 <param name="fasta" value="contig.fasta"/> |
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366 </section> |
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367 <section name="remove_options"> |
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368 <param name="remove_selector" value="--all"/> |
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369 </section> |
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370 </conditional> |
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371 <output name="blobdir"> |
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372 <assert_contents> |
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373 <has_size value="620" delta="50"/> |
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374 </assert_contents> |
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375 </output> |
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376 </test> |
0
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377 </tests> |
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378 <help><![CDATA[ |
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379 BlobToolKit is a software suite to aid researchers in identifying and isolating non-target data in draft and publicly available genome assemblies. It can be used to process assembly, |
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380 read and analysis files for fully reproducible interactive exploration in the browser-based Viewer. BlobToolKit can be used during assembly to filter non-target DNA, helping researchers produce assemblies with high biological credibility. |
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381 |
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382 .. class:: infomark |
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383 |
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384 **NCBI taxdump directory** |
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385 |
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386 The taxdump database, provided by NCBI, includes the taxonomic lineage of taxa, information on type strains and material, and host information. The file **new_taxdump.tar.gz** can be downloaded from the taxonomy directory on the `FTP site <ftp.ncbi.nlm.nih.gov/pub/taxonomy/new_taxdump/>`_. |
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387 |
0
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388 ]]></help> |
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389 <expand macro="citations"/> |
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390 </tool> |