Mercurial > repos > bgruening > deeptools
annotate heatmapper.xml @ 30:5231f398d784 draft
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 3bc1d1c6f4e28ac7ff8df79fe4e3f00a195938e6-dirty
author | bgruening |
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date | Tue, 20 Oct 2015 14:43:12 -0400 |
parents | 3a2aab18a217 |
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29 | 1 <tool id="deeptools_heatmapper" name="heatmapper" version="@WRAPPER_VERSION@.0"> |
2 <description>creates a heatmap for a score associated to genomic regions</description> | |
3 <macros> | |
4 <token name="@BINARY@">heatmapper</token> | |
5 <import>deepTools_macros.xml</import> | |
6 </macros> | |
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7 <expand macro="requirements"/> |
29 | 8 <command> |
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9 <![CDATA[ |
29 | 10 heatmapper |
11 | |
12 --matrixFile $matrixFile | |
13 --outFileName $outFileName | |
14 | |
15 #if $output.showOutputSettings == "yes" | |
16 --plotFileFormat $output.outFileFormat | |
17 #if $outFileNameData: | |
18 --outFileNameData '$outFileNameData' | |
19 #end if | |
20 | |
21 #if $outFileNameMatrix: | |
22 --outFileNameMatrix '$outFileNameMatrix' | |
23 #end if | |
24 | |
25 #if $outFileSortedRegions: | |
26 --outFileSortedRegions '$outFileSortedRegions' | |
27 #end if | |
28 #else | |
29 --plotFileFormat 'png' | |
30 #end if | |
31 | |
32 #if $advancedOpt.showAdvancedOpt == "yes" | |
33 #if $advancedOpt.sortRegions: | |
34 --sortRegions '$advancedOpt.sortRegions' | |
35 #end if | |
36 | |
37 #if $advancedOpt.sortUsing: | |
38 --sortUsing '$advancedOpt.sortUsing' | |
39 #end if | |
40 | |
41 #if $advancedOpt.averageTypeSummaryPlot: | |
42 --averageTypeSummaryPlot '$advancedOpt.averageTypeSummaryPlot' | |
43 #end if | |
44 | |
45 #if str($advancedOpt.missingDataColor.value) != "None": | |
46 --missingDataColor '$advancedOpt.missingDataColor' | |
47 #end if | |
48 | |
49 --colorMap '$advancedOpt.colorMap' | |
50 | |
51 #if str($advancedOpt.zMin).strip() != "": | |
52 --zMin $advancedOpt.zMin | |
53 #end if | |
54 #if $advancedOpt.zMax: | |
55 --zMax $advancedOpt.zMax | |
56 #end if | |
57 | |
58 #if str($advancedOpt.yMin).strip() != "": | |
59 --yMin $advancedOpt.yMin | |
60 #end if | |
61 #if $advancedOpt.yMax: | |
62 --yMax $advancedOpt.yMax | |
63 #end if | |
64 | |
65 --xAxisLabel '$advancedOpt.xAxisLabel' | |
66 --yAxisLabel '$advancedOpt.yAxisLabel' | |
67 | |
68 --heatmapWidth $advancedOpt.heatmapWidth | |
69 --heatmapHeight $advancedOpt.heatmapHeight | |
70 | |
71 --whatToShow '$advancedOpt.whatToShow' | |
72 | |
73 --startLabel '$advancedOpt.startLabel' | |
74 --endLabel '$advancedOpt.endLabel' | |
75 --refPointLabel '$advancedOpt.referencePointLabel' | |
76 --regionsLabel '$advancedOpt.regionsLabel' | |
77 | |
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78 #if $advancedOpt.plotTitle and str($advancedOpt.plotTitle.value) != "": |
29 | 79 --plotTitle '$advancedOpt.plotTitle' |
80 #end if | |
81 | |
82 $advancedOpt.onePlotPerGroup | |
83 | |
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84 @KMEANS_CLUSTERING@ |
29 | 85 |
86 #end if | |
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87 ]]> |
29 | 88 </command> |
89 <inputs> | |
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90 <param name="matrixFile" format="deeptools_compute_matrix_archive" type="data" label="Matrix file from the computeMatrix tool"/> |
29 | 91 |
92 <expand macro="input_graphic_output_settings"> | |
93 <expand macro="input_image_file_format" /> | |
94 <expand macro="input_save_matrix_values" /> | |
95 </expand> | |
96 | |
97 <conditional name="advancedOpt" > | |
98 <param name="showAdvancedOpt" type="select" label="Show advanced options" > | |
99 <option value="no" selected="true">no</option> | |
100 <option value="yes">yes</option> | |
101 </param> | |
102 <when value="no" /> | |
103 <when value="yes"> | |
104 <param name="sortRegions" type="select" label="Sort regions" | |
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105 help="Whether the heatmap should present the regions sorted. The default is to sort in descending order based on the mean value per region. (--sortRegions)"> |
29 | 106 <option value="no">no ordering</option> |
107 <option value="descend" selected="true">descending order</option> | |
108 <option value="ascend">ascending order</option> | |
109 </param> | |
110 | |
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111 <param name="sortUsing" type="select" label="Method used for sorting" |
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112 help="For each row the method is computed. (--sortUsing)"> |
29 | 113 <option value="mean" selected="true">mean</option> |
114 <option value="median">median</option> | |
115 <option value="min">min</option> | |
116 <option value="max">max</option> | |
117 <option value="sum">sum</option> | |
118 <option value="region_length">region length</option> | |
119 </param> | |
120 | |
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121 <param name="averageTypeSummaryPlot" type="select" |
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122 label="Type of statistic that should be plotted in the summary image above the heatmap" |
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123 help="(--averageTypeSummaryPlot)"> |
29 | 124 <option value="mean" selected="true">mean</option> |
125 <option value="median">median</option> | |
126 <option value="min">min</option> | |
127 <option value="max">max</option> | |
128 <option value="sum">sum</option> | |
129 <option value="std">std</option> | |
130 </param> | |
131 | |
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132 <param name="missingDataColor" type="text" value="black" optional="true" label="Missing data color" |
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133 help="If 'Represent missing data as zero' is not set, such cases will be colored in black by default. |
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134 By using this parameter a different color can be set. A value between 0 and 1 will be used for a gray scale (black is 0). |
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135 Also color names can be used, see a list here: http://packages.python.org/ete2/reference/reference_svgcolors.html. |
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136 Alternatively colors can be specified using the #rrggbb notation. (--missingDataColor)" /> |
29 | 137 |
138 <expand macro="colormap" /> | |
139 | |
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140 <param name="zMin" type="float" value="" size="3" |
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141 label="Minimum value for the heatmap intensities. Leave empty for automatic values" help="(--zMin)"/> |
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142 <param name="zMax" type="float" value="" size="3" |
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143 label="Maximum value for the heatmap intensities. Leave empty for automatic values" help="(--zMax)"/> |
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144 <param name="yMin" type="float" value="" size="3" |
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145 label="Minimum value for the Y-axis of the summary plot. Leave empty for automatic values" help="(--yMin)"/> |
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146 <param name="yMax" type="float" value="" size="3" |
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147 label="Maximum value for Y-axis of the summary plot. Leave empty for automatic values" help="(--yMax)"/> |
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148 <param name="xAxisLabel" type="text" value="distance from TSS (bp)" size="200" |
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149 label="Description for the x-axis label" help="(--xAxisLabel)"/> |
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150 <param name="yAxisLabel" type="text" value="genes" size="30" |
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151 label="Description for the y-axis label for the top panel" help="(--yAxisLabel)"/> |
29 | 152 |
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153 <param name="heatmapWidth" type="float" value="7.5" min="1" max="100" |
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154 label="Heatmap width in cm" help="The minimum value is 1 and the maximum is 100."/> |
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155 <param name="heatmapHeight" type="float" value="25" min="3" max="100" |
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156 label="Heatmap height in cm" help="The minimum value is 3 and the maximum is 100."/> |
29 | 157 |
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158 <param name="whatToShow" type="select" label="What to show" |
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159 help ="The default is to include a summary or profile plot on top of the heatmap and a heatmap colorbar. (--whatToShow)"> |
29 | 160 <option value="plot, heatmap and colorbar" selected="true">summary plot, heatmap and colorbar</option> |
161 <option value="plot and heatmap">summary plot and heatmap (no colorbar)</option> | |
162 <option value="heatmap only">heatmap only</option> | |
163 <option value="heatmap and colorbar">heatmap and colorbar</option> | |
164 <option value="colorbar only">colorbar only</option> | |
165 </param> | |
166 | |
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167 <param name="startLabel" type="text" value="TSS" size="10" |
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168 label="Label for the region start" |
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169 help ="Only for scale-regions mode. Label shown in the plot for the start of the region. Default is TSS (transcription start site), but could be changed to anything, e.g. "peak start". (--startLabel)" /> |
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170 <param name="endLabel" type="text" value="TES" size="10" |
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171 label="Label for the region end" |
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172 help="Only for scale-regions mode. Label shown in the plot for the region end. Default is TES (transcription end site). (--endLabel)"/> |
29 | 173 |
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174 <param name="referencePointLabel" type="text" value="TSS" size="10" |
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175 label="Reference point label" |
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176 help ="Label shown in the plot for the reference-point. Default is the same as the reference point selected (e.g. TSS), but could be anything, e.g. "peak start" etc. (--referencePointLabel)" /> |
29 | 177 <param name="regionsLabel" type="text" value="genes" size="30" |
178 label="Labels for the regions plotted in the heatmap" | |
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179 help="If more than one region is being plotted a list of labels separated by comma and limited by quotes, is required. For example, label1, label2. (--regionsLabel)"> |
29 | 180 <sanitizer> |
181 <valid initial="string.printable"> | |
182 </valid> | |
183 </sanitizer> | |
184 </param> | |
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185 <expand macro="plotTitle" /> |
29 | 186 <param name="onePlotPerGroup" type="boolean" truevalue="--onePlotPerGroup" falsevalue="" |
187 label="Do one plot per group" | |
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188 help="When computeMatrix was used on more than one group of genes, the average plots for all the groups will be drawn in one panel by default. |
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189 If this option is set, each group will get its own plot, stacked on top of each other. (--onePlotPerGroup)"/> |
29 | 190 |
191 <expand macro="kmeans_clustering" /> | |
192 </when> | |
193 </conditional> | |
194 </inputs> | |
195 <outputs> | |
196 <expand macro="output_image_file_format" /> | |
197 <expand macro="output_graphic_outputs" /> | |
198 <expand macro="output_save_matrix_values" /> | |
199 </outputs> | |
200 <tests> | |
201 <test> | |
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202 <param name="matrixFile" value="computeMatrix_result1.gz" ftype="deeptools_compute_matrix_archive" /> |
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203 <output name="outFileName" file="heatmapper_result1.png" ftype="png" compare="sim_size" delta="100" /> |
29 | 204 </test> |
205 </tests> | |
206 <help> | |
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207 <![CDATA[ |
29 | 208 **What it does** |
209 | |
210 The heatmapper visualizes scores associated with genomic regions, for example ChIP enrichment values around the TSS of genes. | |
211 Like profiler, it requires that computeMatrix was run first to calculate the values. | |
212 | |
213 We implemented vast optional parameters to optimize the visual output and we encourage you to play around with the min/max values displayed in the heatmap as well as | |
214 with the different coloring options. The most powerful option is the k-means clustering where you simply need to indicate the number of | |
215 groups with similar read distributions that you expect and the algorithm will do the sorting for you. | |
216 | |
217 Do check the examples on our help page with step-by-step protocols: https://github.com/fidelram/deepTools/wiki/Example-workflows | |
218 | |
219 | |
220 .. image:: $PATH_TO_IMAGES/visual_hm_DmelPolII.png | |
221 :alt: Heatmap of RNA Polymerase II ChIP-seq | |
222 | |
223 | |
224 You can find more details on the tool itself on the heatmapper wiki page: https://github.com/fidelram/deepTools/wiki/Visualizations#wiki-heatmapper | |
225 | |
226 | |
227 ----- | |
228 | |
229 @REFERENCES@ | |
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230 ]]> |
29 | 231 </help> |
232 <expand macro="citations" /> | |
233 </tool> |