Mercurial > repos > bgruening > diffbind
changeset 14:c97a786e8fb5 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/diffbind commit 1fe103338548dd65731a5a0887be59745370debd
| author | iuc |
|---|---|
| date | Thu, 07 Jun 2018 05:21:22 -0400 |
| parents | 1de83981d43c |
| children | 194e3f2c1d86 |
| files | diffbind.xml test-data/input1.bam test-data/input2.bam test-data/out_diffbind_ctrl.interval |
| diffstat | 4 files changed, 45 insertions(+), 15 deletions(-) [+] |
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--- a/diffbind.xml Wed May 30 12:25:42 2018 -0400 +++ b/diffbind.xml Thu Jun 07 05:21:22 2018 -0400 @@ -1,4 +1,4 @@ -<tool id="diffbind" name="DiffBind" version="2.6.6.3"> +<tool id="diffbind" name="DiffBind" version="2.6.6.4"> <description> differential binding analysis of ChIP-Seq peak data</description> <requirements> <requirement type="package" version="2.6.6">bioconductor-diffbind</requirement> @@ -37,16 +37,18 @@ #set $bam_controls = list() #for $file in $g.peaks: - #set $file_name = re.sub('[^\w\-\s]', '_', str($file.element_identifier)) - ln -s '${file}' ${g.groupName}-${file_name}-peaks.bed && - $peak_files.append(str($g.groupName) + '-' + $file_name + '-peaks.bed') + #set $file_name = str($g.groupName) + "-" + re.sub('[^\w\-]', '_', str($file.element_identifier)) + "-peaks.bed" + ln -s '${file}' '${file_name}' && + $peak_files.append($file_name) #end for #for $bam in $g.bamreads: - #set $bam_name = re.sub('[^\w\-\s]', '_', str($bam.element_identifier)) - ln -s '${bam}' ${bam_name}-bamreads.bam && - ln -s ${bam.metadata.bam_index} ${bam_name}-bamreads.bai && - $bam_files.append($bam_name + '-bamreads.bam') + #set $bam_name = re.sub('[^\w\-]', '_', str($bam.element_identifier)) + #set $bam_file = $bam_name + "-bamreads.bam" + #set $bam_index = $bam_name + "-bamreads.bai" + ln -s '${bam}' '${bam_file}' && + ln -s '${bam.metadata.bam_index}' '${bam_index}' && + $bam_files.append($bam_file) #end for $temp_factor.append( {str($g.groupName): $peak_files} ) @@ -54,10 +56,14 @@ #if str( $g.bamcontrol ) != 'None': #for $ctrl in $g.bamcontrol: - #set $ctrl_name = re.sub('[^\w\-\s]', '_', str($ctrl.element_identifier)) - ln -s '${ctrl}' ${g.groupName}-${ctrl_name}-bamcontrol.bam && - ln -s ${ctrl.metadata.bam_index} ${g.groupName}-${ctrl_name}-bamcontrol.bai && - $bam_controls.append(str($g.groupName) + '-' + $ctrl_name + '-bamcontrol.bam') + #set $ctrl_name = re.sub('[^\w\-]', '_', str($ctrl.element_identifier)) + #set $ctrl_file = $ctrl_name + "-bamcontrol.bam" + #set ctrl_index = $ctrl_name + "-bamcontrol.bai" + #if $ctrl_file not in json.dumps($temp_factor): + ln -s '${ctrl}' '${ctrl_file}' && + ln -s '${ctrl.metadata.bam_index}' '${ctrl_index}' && + #end if + $bam_controls.append($ctrl_file) #end for $temp_factor.append( {str($g.groupName): $bam_controls} ) #end if @@ -113,8 +119,8 @@ <validator type="empty_field" /> </param> <param name="peaks" type="data" format="bed" multiple="true" label="Peak files" help="Result of your Peak calling experiment"/> - <param name="bamreads" type="data" format="bam" multiple="true" label="Read BAM file" help="Specify the Read BAM file used in the Peak calling."/> - <param name="bamcontrol" type="data" format="bam" multiple="true" optional="True" label="Control BAM file" help="If specifying a control BAM file, all samples are required to specify one, see Help section below."/> + <param name="bamreads" type="data" format="bam" multiple="true" label="Read BAM files" help="Specify the Read BAM files used in the Peak calling. The input order of the BAM files for the samples MUST match the input order of the peaks files."/> + <param name="bamcontrol" type="data" format="bam" multiple="true" optional="True" label="Control BAM files" help="If specifying a control BAM file, all samples are required to specify one, see Help section below. The input order of the BAM files for the samples MUST match the input order of the peaks files."/> </repeat> <param name="scorecol" type="integer" min="0" value="8" label="Score Column" help="Column in peak files that contains peak scores. Default: 8 (narrowPeak)"> @@ -204,6 +210,24 @@ </assert_contents> </output> </test> + <!-- Ensure control BAMs input works --> + <test expect_num_outputs="1"> + <repeat name="rep_group"> + <param name="groupName" value="Resistant"/> + <param name="peaks" ftype="bed" value="BT474_ER_1.bed.gz,BT474_ER_2.bed.gz"/> + <param name="bamreads" ftype="bam" value="BT474_ER_1.bam,BT474_ER_2.bam" /> + <param name="bamcontrol" ftype="bam" value="input1.bam,input2.bam" /> + </repeat> + <repeat name="rep_group"> + <param name="groupName" value="Responsive"/> + <param name="peaks" ftype="bed" value="MCF7_ER_1.bed.gz,MCF7_ER_2.bed.gz"/> + <param name="bamreads" ftype="bam" value="MCF7_ER_1.bam,MCF7_ER_2.bam" /> + <param name="bamcontrol" ftype="bam" value="input1.bam,input2.bam" /> + </repeat> + <param name="scorecol" value="5" /> + <param name="format" value="interval"/> + <output name="outfile" ftype="interval" value="out_diffbind_ctrl.interval" /> + </test> <!-- Ensure BED output works --> <test expect_num_outputs="1"> <repeat name="rep_group"> @@ -266,7 +290,7 @@ as well as comparing the results of an occupancy-based analysis with an affinity-based one. Finally, certain technical aspects of the how these analyses are accomplished are detailed. -Note this DiffBind tool requires a minimum of four samples (two groups with two replicates each). +**Note this DiffBind tool requires a minimum of four samples (two groups with two replicates each).** -----
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out_diffbind_ctrl.interval Thu Jun 07 05:21:22 2018 -0400 @@ -0,0 +1,6 @@ +Chrom Start End Name Score Strand Comment +chr18 394599 396513 DiffBind 0 . 1914|6.86|3.93|7.76|-3.83|2.05e-37|2.85e-34 +chr18 399013 400382 DiffBind 0 . 1369|7.47|6.71|7.96|-1.25|2.31e-09|1.61e-06 +chr18 346463 347342 DiffBind 0 . 879|4.18|5.16|-0.93|6.09|1.1e-07|5.13e-05 +chr18 111566 112005 DiffBind 0 . 439|5.55|6.41|3.03|3.38|5.03e-06|0.00175 +chr18 371109 372102 DiffBind 0 . 993|4.04|-0.02|5|-5.02|1.23e-05|0.00344