Mercurial > repos > bgruening > itsx
changeset 0:2cbd0d66aa08 draft default tip
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/itsx commit 229c8c8ce97a8e4d3d46406dba2942135fbdb910"
| author | bgruening |
|---|---|
| date | Mon, 02 May 2022 13:03:49 +0000 |
| parents | |
| children | |
| files | itsx.xml macros.xml test-data/metagenomics.fasta test-data/test_01_found.tabular test-data/test_01_its2.fasta test-data/test_01_not_detected.fasta test-data/test_01_not_detected.txt test-data/test_01_problematic.tabular test-data/test_01_summary.txt test-data/test_02_found.tabular test-data/test_02_its2.fasta test-data/test_02_not_detected.fasta test-data/test_02_not_detected.txt test-data/test_02_problematic.tabular test-data/test_02_summary.txt |
| diffstat | 15 files changed, 1354 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/itsx.xml Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,353 @@ +<tool id="itsx" name="ITSx" version="@TOOL_VERSION@+galaxy@SUFFIX_VERSION@" profile="20.01"> + <description>identifies ITS sequences and extracts the ITS region</description> + <macros> + <import>macros.xml</import> + </macros> + <xrefs> + <xref type="bio.tools">ITSx</xref> + </xrefs> + <expand macro="requirements" /> + <version_command>ITSx --license</version_command> + <command detect_errors="exit_code"><![CDATA[ + ITSx + --cpu \${GALAXY_SLOTS:-4} + -i '$i' + --save_regions #echo ",".join($output_options.save_regions) + --only-output $output_options.only_output + --partial $output_options.partial + --minlen $output_options.minlen + --truncate $output_options.truncate + -t #echo ",".join($sequence_section.character_code) + -E $sequence_section.E + -S $sequence_section.S + -N $sequence_section.N + --selection_priority $sequence_section.selection_priority + --allow_single_domain $sequence_section.allow_single_domain + --allow_reorder $sequence_section.allow_reorder + --complement $sequence_section.complement + --heuristics $sequence_section.heuristics + --nhmmer $sequence_section.nhmmer + #if 'summary' in $output_options.output_files + --summary T + #else + --summary F + #end if + #if 'graphical' in $output_options.output_files + --graphical T + #else + --graphical F + #end if + #if 'fasta' in $output_options.output_files + --fasta T + #else + --fasta F + #end if + #if 'concat' in $output_options.output_files + --concat T + #else + --concat F + #end if + #if 'table' in $output_options.output_files + --table T + #else + --table F + #end if + #if 'not_found' in $output_options.output_files + --not_found T + #else + --not_found F + #end if + #if 'detailed_results' in $output_options.output_files + --detailed_results T + #else + --detailed_results F + #end if + #if 'positions' in $output_options.output_files + --positions T + #else + --positions F + #end if + --preserve $output_options.preserve + + ]]></command> + <inputs> + <param argument="-i" type="data" format="fasta" label="FASTA file"/> + <section name="sequence_section" title="Sequence selection options" expanded="true"> + <param name="character_code" argument="-t" type="select" + multiple="true" label="Profile set to use for the search" + help="Can be used to restrict the search to only a few organism groups types to save time, if + one or more of the origins are not relevant to the dataset under study. Default: all"> + <option value="all" selected="true">All</option> + <option value="a">Alveolata</option> + <option value="b">Bryophyta</option> + <option value="c">Bacillariophyta</option> + <option value="d">Amoebozoa</option> + <option value="e">Euglenozoa</option> + <option value="f">Fungi</option> + <option value="g">Chlorophyta</option> + <option value="h">Rhodophyta</option> + <option value="i">Phaeophyceae</option> + <option value="l">Marchantiophyta</option> + <option value="m">Metazoa</option> + <option value="o">Oomycota</option> + <option value="p">Haptophyceae</option> + <option value="q">Raphidophyceae</option> + <option value="r">Rhizaria</option> + <option value="s">Synurophyceae</option> + <option value="t">Tracheophyta</option> + <option value="u">Eustigmatophyceae</option> + <option value="x">Apusozoa</option> + <option value="y">Parabasalia</option> + </param> + <param argument="-E" type="float" min="0" max="1" value="0.00005" label="Domain E-value cutoff" + help="Domain E-value cutoff for a sequence to be included in the output. Default: 1e-5"/> + <param argument="-S" type="float" min="0" max="1" value="0" label="Domain score cutoff" + help="Domain score cutoff for a sequence to be included in the output. Default:0" /> + <param argument="-N" type="integer" min="0" max="20" value="2" label="Minimal number of domains" + help="The minimal number of domains that must match a sequence before it is included. Default:2" /> + <param argument="--selection_priority" type="select" label="Selection priority" help="Selects what will + be of highest priority when determining the origin of the sequence. Default:score"> + <option value="score">Score</option> + <option value="sum">Sum</option> + <option value="domains">Domains</option> + <option value="eval">Eval</option> + </param> + <conditional name="cutoff_conditional"> + <param name="cutoff_mode" type="select" label="HMMER search cutoff mode" help="Default: Score cutoff"> + <option value="eval">E-value cutoff</option> + <option value="score" selected="true">Score cutoff</option> + </param> + <when value="eval"> + <param argument="--search_eval" type="float" min="0" max="1" value="" optional="true" + label="E-value cutoff used in the HMMER search" + help="High numbers may slow down the process" /> + </when> + <when value="score"> + <param argument="--search_score" type="float" min="0" max="1" value="" optional="true" + label="The score cutoff used in the HMMER search" + help="Low numbers may slow down the process" /> + </when> + </conditional> + <param argument="--allow_single_domain" type="float" min="0" max="1" value="0" label="Allow single domain" + help="Allow inclusion of sequences that only find a single domain, given that they meet the given E-value and score thresholds. Default: 0" /> + <param argument="--allow_reorder" type="boolean" truevalue="T" falsevalue="F" checked="false" label="Allow reorder" + help="Allows profiles to be in the wrong order on extracted sequences" /> + <param argument="--complement" type="boolean" truevalue="T" falsevalue="F" checked="true" label="Check both DNA strains" + help="Checks both DNA strands against the database, creating reverse complements" /> + <param argument="--heuristics" type="boolean" truevalue="T" falsevalue="F" checked="false" + label="HMMER's heuristic filtering" help="Leave this setting off for higher precision" /> + <param argument="--nhmmer" type="boolean" truevalue="T" falsevalue="F" checked="false" label="Use nhmmer instead of hmmsearch" help="Default: No" /> + </section> + <section name="output_options" title="Output options"> + <param name="output_files" type="select" multiple="true" display="checkboxes" label="Output files"> + <option value="summary" selected="true">Summary</option> + <option value="graphical">Graphical output</option> + <option value="fasta" selected="true">FASTA-format of extracted ITS sequences</option> + <option value="concat">Concateneted ITS sequences</option> + <option value="positions" selected="true">Positions of found IT sequences</option> + <option value="table" selected="true">Positions of probable IT sequences</option> + <option value="not_found" selected="true">List of not-found entries</option> + <option value="detailed_results">Table of all results</option> + </param> + <param argument="--preserve" type="boolean" truevalue="T" falsevalue="F" checked="false" label="Preserve sequence headers in input file" help="Preserve sequence headers in input file instead of printing out ITSx headers. Default: No" /> + <param argument="--save_regions" type="select" label="Save regions" multiple="true" help="A comma separated list of regions to output separate FASTA files"> + <option value="none">None</option> + <option value="SSU">SSU</option> + <option value="ITS1" selected="true">ITS1</option> + <option value="ITS2" selected="true">ITS2</option> + <option value="5.8S">5.8S</option> + <option value="LSU">LSU</option> + <option value="all">All</option> + </param> + <param argument="--only_output" type="boolean" truevalue="T" falsevalue="F" checked="false" label="Only full-lenght regions" help="If enabled, output is limited to full-length regions. Default: no" /> + <param argument="--partial" type="integer" min="0" value="0" label="Saves additional FASTA-files for full and partial ITS sequences longer than the specified cutoff" help="Default: 0 (disabled)" /> + <param argument="--minlen" type="integer" min="0" value="0" label="Minimum length the ITS regions must be to be outputted in the concatenated file" help="Default: 0" /> + <param argument="--truncate" type="boolean" truevalue="T" falsevalue="F" checked="false" label="Truncates the FASTA output to only contain the actual ITS sequences found" help="Default: yes" /> + </section> + </inputs> + <outputs> + <data name="not_detection_fasta" format="fasta" from_work_dir="ITSx_out_no_detections.fasta" label="${tool.name} on ${on_string}: no detection (FASTA)"> + <filter> output_options['output_files'] and 'not_found' in output_options['output_files']</filter> + </data> + <data name="not_detection_txt" format="txt" from_work_dir="ITSx_out_no_detections.txt" label="${tool.name} on ${on_string}: no detection (TXT)"> + <filter> output_options['output_files'] and 'not_found' in output_options['output_files']</filter> + </data> + <data name="its1" format="fasta" from_work_dir="ITSx_out.ITS1.fasta" label="${tool.name} on ${on_string}: ITS1"> + <filter>output_options['output_files'] and 'fasta' in output_options['output_files']</filter> + <filter>output_options['save_regions'] and 'ITS1' in output_options['save_regions']</filter> + </data> + <data name="its2" format="fasta" from_work_dir="ITSx_out.ITS2.fasta" label="${tool.name} on ${on_string}: ITS2"> + <filter>output_options['output_files'] and 'fasta' in output_options['output_files']</filter> + <filter>output_options['save_regions'] and 'ITS2' in output_options['save_regions']</filter> + </data> + <data name="ribosomall58" format="fasta" from_work_dir="ITSx_out.5_8S.fasta" label="${tool.name} on ${on_string}: 5.8S"> + <filter>output_options['output_files'] and 'fasta' in output_options['output_files']</filter> + <filter>output_options['save_regions'] and '5.8S' in output_options['save_regions']</filter> + </data> + <data name="LSU" format="fasta" from_work_dir="ITSx_out.LSU.fasta" label="${tool.name} on ${on_string}: LSU"> + <filter>output_options['output_files'] and 'fasta' in output_options['output_files']</filter> + <filter>output_options['save_regions'] and 'LSU' in output_options['save_regions']</filter> + </data> + <data name="SSU" format="fasta" from_work_dir="ITSx_out.SSU.fasta" label="${tool.name} on ${on_string}: SSU"> + <filter>output_options['output_files'] and 'fasta' in output_options['output_files']</filter> + <filter>output_options['save_regions'] and 'SSU' in output_options['save_regions']</filter> + </data> + <data name="full_sequences" format="fasta" from_work_dir="ITSx_out.full.fasta" label="${tool.name} on ${on_string}: full sequences"> + <filter>output_options['output_files'] and 'fasta' in output_options['output_files']</filter> + </data> + <data name="concat" format="fasta" from_work_dir="ITSx_out.concat.fasta" label="${tool.name} on ${on_string}: concatenated sequences"> + <filter>output_options['output_files'] and 'concat' in output_options['output_files']</filter> + </data> + <data name="graph" format="txt" from_work_dir="ITSx_out.graph" label="${tool.name} on ${on_string}: graphical representation"> + <filter>output_options['output_files'] and 'graph' in output_options['output_files']</filter> + </data> + <data name="summary" format="txt" from_work_dir="ITSx_out.summary.txt" label="${tool.name} on ${on_string}: summary"> + <filter>output_options['output_files'] and 'summary' in output_options['output_files']</filter> + </data> + <data name="detailed_results" format="tabular" from_work_dir="ITSx_out.extraction.results" label="${tool.name} on ${on_string}: detailed results"> + <filter>output_options['output_files'] and 'detailed_results' in output_options['output_files']</filter> + </data> + <data name="positions_probable" format="tabular" from_work_dir="ITSx_out.hmmer.table" label="${tool.name} on ${on_string}: probable ITS positions"> + <filter>output_options['output_files'] and 'table' in output_options['output_files']</filter> + </data> + <data name="positions_found" format="tabular" from_work_dir="ITSx_out.positions.txt" label="${tool.name} on ${on_string}: positions"> + <filter>output_options['output_files'] and 'positions' in output_options['output_files']</filter> + </data> + <data name="problematic" format="tabular" from_work_dir="ITSx_out.problematic.txt" label="${tool.name} on ${on_string}: problematic reads"/> + </outputs> + <tests> + <!--Default parameters--> + <test expect_num_outputs="9"> + <param name="i" value="metagenomics.fasta"/> + <section name="sequence_section"> + <param name="character_code" value="f"/> + <param name="E" value="0.00005"/> + <param name="S" value="0"/> + <param name="N" value="2"/> + <param name="selection_priority" value="score"/> + <param name="allow_single_domain" value="0"/> + <param name="allow_reorder" value="false"/> + <param name="complement" value="true"/> + <param name="heuristics" value="false"/> + <param name="nhmmer" value="false"/> + </section> + <section name="output_options"> + <param name="output_files" value="summary,fasta,positions,table,not_found"/> + <param name="preserve" value="false"/> + <param name="save_regions" value="ITS1,ITS2"/> + <param name="only_output" value="false"/> + <param name="partial" value="0"/> + <param name="minlen" value="0"/> + <param name="truncate" value="false"/> + </section> + <output name="not_detection_fasta" file="test_01_not_detected.fasta" ftype="fasta"/> + <output name="not_detection_txt" file="test_01_not_detected.txt" ftype="txt"/> + <output name="its2" file="test_01_its2.fasta" ftype="fasta"/> + <output name="summary" file="test_01_summary.txt" ftype="txt" lines_diff="4"/> + <output name="positions_found" file="test_01_found.tabular" ftype="tabular"/> + <output name="positions_probable" ftype="tabular"> + <assert_contents> + <has_text text="10088_SRR5572434|r"/> + <has_text text="19705_SRR5572434|fr"/> + </assert_contents> + </output> + <output name="problematic" file="test_01_problematic.tabular" ftype="tabular"/> + </test> + <!--Custom options--> + <test expect_num_outputs="9"> + <param name="i" value="metagenomics.fasta"/> + <section name="sequence_section"> + <param name="character_code" value="f"/> + <param name="E" value="0.0005"/> + <param name="S" value="1"/> + <param name="N" value="2"/> + <param name="selection_priority" value="score"/> + <param name="allow_single_domain" value="0.1"/> + <param name="allow_reorder" value="true"/> + <param name="complement" value="true"/> + <param name="heuristics" value="true"/> + <param name="nhmmer" value="false"/> + </section> + <section name="output_options"> + <param name="output_files" value="summary,fasta,positions,table,not_found"/> + <param name="preserve" value="false"/> + <param name="save_regions" value="ITS1,ITS2"/> + <param name="only_output" value="false"/> + <param name="partial" value="0"/> + <param name="minlen" value="20"/> + <param name="truncate" value="true"/> + </section> + <output name="not_detection_fasta" file="test_02_not_detected.fasta" ftype="fasta"/> + <output name="not_detection_txt" file="test_02_not_detected.txt" ftype="txt"/> + <output name="its2" file="test_02_its2.fasta" ftype="fasta"/> + <output name="summary" file="test_02_summary.txt" ftype="txt" lines_diff="4"/> + <output name="positions_found" file="test_02_found.tabular" ftype="tabular"/> + <output name="positions_probable" ftype="tabular"> + <assert_contents> + <has_text text="10088_SRR5572434|r"/> + <has_text text="19705_SRR5572434|fr"/> + </assert_contents> + </output> + <output name="problematic" file="test_02_problematic.tabular" ftype="tabular"/> + </test> + </tests> + <help><![CDATA[ + +.. class:: infomark + +**Purpose** + +ITSx is an open source software utility to extract the highly variable ITS1 and ITS2 subregions from ITS sequences, which is commonly used as a molecular barcode for e.g. fungi. As the inclusion of parts of the neighbouring, +very conserved, ribosomal genes (SSU, 5S and LSU rRNA sequences) in the sequence identification process can lead to severely misleading results, ITSx identifies and extracts only the ITS regions themselves. + +ITSx accepts input in the FASTA format. As it pre-processes the input sequences, it is possible to input both aligned and unaligned FASTA files, containing both DNA and RNA sequences. + +---- + +.. class:: infomark + +**Algorithm and implementation** + +The main design goal for ITSx is to achieve fast and accurate extraction of ITS regions in large data sets, without introducing a large number of false positives. To be able to reach a high speed, ITSx relies on +the HMMER3 software, which allows for extremely fast comparisons of HMM-profiles to a sequence set. To achieve high detection accuracy, ITSx uses multiple HMM-profiles built from the conserved domains flanking +the ITS regions (SSU, 5.8S and LSU), representing a large number of species groups. This enabled ITSx to extract ITS regions from all eukaryote lineages for which a substantial number of reference ITS sequences +were available as of 2012. The 2017 release of 1.1 saw the introduction of additional HMM profiles to cover even more lineages, particularly within the fungi. + +While the default settings of ITSx should be usable in most situations, you should consider if they suitable for your purposes and for your data set. If the data set is small, this can be done by running the +software multiple times on the data, with different settings, and analyse the outcome. On larger data sets, it might be more feasible to only run ITSx on a subset of the sequences for testing. The graphical output +is very useful for determining whether ITSx performs as desired on the data, as the positions of the found conserved domains can be easily investigated. If domains are missing, the criteria might be set to be too +stringent. If they are not in sequential order (from SSU to LSU with the 5.8S in between), that might be an indication that there is something wrong with the input sequences + +---- + +.. class:: infomark + +**Notes on PCR primers and short input sequences** + +One obvious use of ITSx would be to run it on sequence datasets generated by PCR amplicon studies of the ITS region, to extract ITS1 and/or ITS2 regions, and to sort +out non-target sequences in the data prior to further analysis. ITSx uses the conserved SSU, 5S and LSU genes to located and orient the ITS regions. To do this, the +software requires at least ~20 bp. (of at least one) of those genes to be present for each input sequence – preferably 25 bp. However, some primer pairs targeting the ITS +region will not include sufficiently large portions of these genes to be detected with the accuracy required by ITSx by default. This may lead to that fewer, or even none, of +the input sequences are recognized as ITS containing. + + +If the dataset is known to contain only ITS sequences, a remedy to this problem can be to lower the stringency of ITSx, using the -E option. By default, this is set to 1e-5, +but this can be increased to say 0.01, or even 1 to allow for detection of shorter portions of the conserved genes – down to some 15 bases. This feature comes at the +price of an increase proportion of false-positive matches, but in the case of ITS-only datasets this will be less of a concern. Note that this should normally be done only for +datasets that are known to contain only ITS sequences, and that caution needs to be taken in the downstream analysis so that false-positive extractions can be avoided (e.g. +investigate spuriously long or short ITS sequences with a sound degree of scepticism). Conversely, going for very stringent settings may come at the price of sensitivity as +sequences with deviant genes (or of reduced read quality) may be missed. The default settings of the software are calibrated with environmental datasets in mind, to keep +false-positive extractions at a minimum. + +If the problem instead is that only one of those genes is present on the input sequence, another, even more polished, solution exists. In order to score a sequence as an ITS +sequence, ITSx prefers to see that the sequence produces matches to at least two HMMs (such as 3’ SSU and 5’ 5.8S). This helps ITSx to keep the number of falsepositive +identifications low. However, the software also allows sequences that produce a match to only one HMM (such as 3’ SSU) to be scored as ITS sequences, provided +that the match is particularly stringent. The stringency of this parameter is controlled through the --allow_single_domain switch (see above). The default value is 1e-9. If +ITSx is used on very short sequences (e.g. 3’ SSU + 100 bp. ITS1), then only one HMM can be expected to match, and the E-value of that match will be compared to +the value of this parameter. Therefore, if the dataset at hand contains sequences which should only contain a single conserved region, using e.g. --allow_single_domain +"1e-5,0" can be enough to go from no matches to all matches. However, as with the -E option above, increasing the E-value of this parameter comes at the expense of +higher risk for false positives. + + ]]></help> + <expand macro="citations" /> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,14 @@ +<macros> + <token name="@TOOL_VERSION@">1.1.3</token> + <token name="@SUFFIX_VERSION@">0</token> + <xml name="requirements"> + <requirements> + <requirement type="package" version="@TOOL_VERSION@">itsx</requirement> + </requirements> + </xml> + <xml name="citations"> + <citations> + <citation type="doi">10.1111/2041-210X.12073</citation> + </citations> + </xml> +</macros>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/metagenomics.fasta Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,200 @@ +>2150_SRR5572429|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +ACAGTCTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACACAGTC +>4821_SRR5572429|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +ACAGTCTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGNTCCCGCCTGACCNGGGGTCGCGTTAAAGACTNTGGGTCATCGAGAGCATTTGNACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCNAGACGCTCCNAGGCNCCCTCAGCTCAGATTTAGNCAAACCGCGTGCGGTAAACACGGGAGANCAGCTTCCNTCCGGTANCCGCAANGGAAGGGGACGACGATTTGNGACACCCAGGGAGACGTGCCCTCAGNCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGATTCGATGACTCACACAGTC +>7808_SRR5572429|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +ACAGTCTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTAGTCCTACCTGATTTGAGATCAGATCATAAAGGTATTGTCCAAGCCAATGGACTATTGGAAGCGGTTCATCTGCATTTACCTTGGCCACCTTTTTACAGTGTCCTCAGCGATAGATAATTTATCACGCCGAGAGGAACCAAGCATAACACTGAGATCCAGCTAATGCACAAAGAGGAGCAGGTGTGAAGCTGCAAAGGACCTCCAATACCAAAGTGAAACCAATTGAATTAACAAAAGATTTACTTTGAAGAATTCATGATACTCAAACAGGCATGCTCCAAGGAATACCAAGGAGCGCAAGGTGCGTTCAAAGACTCGATGACTCACACAGTC +>17841_SRR5572429|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GACTGTGTGAGTCATCGAGTCTTTGAACGCAACTTGCGCTCAATGGTATTCCATTGAGCACGCCTGTTTCAGTATCAAAAACACCCCACATTCATAATTTTGTTGTGAATGGAAATGAGAGTTTCGGCTTTATTGCTGAATTCTTTAAAATTATTAGGCCTGAACTATTGTTCTTTCTGCCTGAACATTTTTTTAATATAAAGGAATGCTCTAGTAAAAAGACTATCTCTGGGGCCTCCCAAATAAATCATTCTTAAATTTGATCTGAAGTCAGGCGGGATTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAGACTGT +>10097_SRR5572430|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAGGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>10297_SRR5572430|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCTTGGAGCATGCCTGTTTGAGTATCATGAATTCTTCAAAGTAAATCATTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTGTGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATGATCTGATCTCAAATCAGGTAGGACTACCCGCTGAACTTAGGCATATCAATAAGCGGAGGATAGCT +>16217_SRR5572430|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGATTCACAGCTA +>24991_SRR5572430|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAATCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>37210_SRR5572430|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGACCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>38583_SRR5572430|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGATTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTCTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCAACCGCTGAGTTTTAGCATATCAATAAGCGGAGGATAGCT +>40166_SRR5572430|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>55631_SRR5572430|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCTGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGATTCGATGATTCACAGCTA +>67029_SRR5572430|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>70284_SRR5572430|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +AGCTATCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTAGTCCTACCTGATTTGAGATCAGATCATAAAGGTATTGTCCAAGTCAATGGACTATTAGAAGCGGTTCATCTGCATTTACCTTGGCCACCTTTTACAGTGTCCTCAGCGATAGATAATTTATCACGCCGAGTGGAACCAAGCATAACACTGAGATCCAGCTAATGAACAAAGAGGAGCAGGTGTGAAGCTGCAAAAGACCTCCAATACCAAAGTGAAACCAATTGAGTTAACAAATGATTTACTTTGAAGAATTCATGATACTCAAACAGGCATGCTCCAAGGAATACCAAGGAGCGCAAGGTGCGTTCAAAGACTCGATGACTCACAGCTA +>72280_SRR5572430|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGGCATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>82941_SRR5572430|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACAGCTA +>110667_SRR5572430|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGGCGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>116764_SRR5572430|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCTCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCACTTGGACAGGNGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGAGGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGNAGGCTTGGGGCGCAACTTGCGTGCAAAGAATCGATGACTCACAGCTA +>119907_SRR5572430|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACAGCTA +>133359_SRR5572430|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAGGGCTTGGGGCGCAACTTGTGTTCAAAGACTCGCTGACTCACAGCTA +>151248_SRR5572430|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAGTCATCGAGTCTTTGAACGCACCTTGCGCTCCTTGGTATTCCTTGGAGCATGCCTGTTTGAGTATCATGAATTCTTCAAAGTAAATCTTTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCTTTGACTTGGACAATACCTTTATGATCTGATCTCAAATCAGGTAGGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGATAGCT +>152008_SRR5572430|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACAGCTA +>155395_SRR5572430|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAGTCATCGAGTCTTTGAACGCACCTTGCGCTCCTTGGTATTCCTTGGAGCATGCCTGTTTGAGTATCATGAAATTCTCAAAGTAAACCTTTTGTTAATTCAACTGGTCTTTGCTTTGGATTTGGAGGCCTCTGCAGATGTCACAGTCTGCTCCTCTTAAATAAATTAGCTGGATCTCAGTATACACTTGGTTCCACTCGGCGTGATAAGTATCACTCGCTGAGGACACTGTTAAAAAGGTGGCCAGGCAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAAATTAAAAATTTATGATCTGATCTCAAATCAGGTAGGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGATAGCT +>8277_SRR5572431|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GTCGCGTGAGTCATCGAGTCTTTGAACGCAAGTTGCNCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGNCGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGNNGGTGAAACAAANCCTCGTCATTTTGTCGGTCGCTCNTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGNCNGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGTCGC +>11225_SRR5572431|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +GCGACTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACGCGAC +>16084_SRR5572431|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GTCGCGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAACGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGTCGC +>32756_SRR5572431|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +GCGACTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCANCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCANAGGATGGGGACGNCGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGGCTCACGCGAC +>38016_SRR5572431|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +GCGACTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTACCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACGCGAC +>57950_SRR5572431|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GTCGCGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCAGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGTCGC +>23021_SRR5572432|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +CGTCGAGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACGTCGA +>26380_SRR5572432|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +CGTCGAGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGATGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTNCATATGCTCTCGATGACACAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACGTCGA +>52798_SRR5572432|fr bdiffs=0(match) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAGGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGAGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGNCGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>53558_SRR5572432|fr bdiffs=0(match) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +TCGACGTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTAGTCTCACTTCATTTCAGGTCTTAGTCGAAAACAGCACACACAAGTGTGTGGATTTGATCGGCAGTCTGTACACACGAGGCGCACAAACGCAAATCAAAAACAAGCGAATCAGCCTGTTTGTCCTCAAAAGAGAGGACTCACAGACGGTGCACACGGCGCAATACTCCTTTCCTAAACACTAACCACGTATTGGATTCAAGACCAAAAGAGATCGAGTATCCACGTCGCTAGCGAGTACAATCGGAATACTGTCCGCGAAGAGCAATACACTGAAGCCTTGCCACATTTGAAGGGACACCAAAGAGTTAACTCGGTGACCGACTCAATCTCCAGATGCGCAGTCGCAATGAAGAGATGACGCCGAGGTGTGGTTGAAACGACTCTGAAAGAAGCGTACTGTCAGGCCTATCCCGACAGTGTCATTTGCGTTCAAAGACTCGATGATTCACTCGACG +>57614_SRR5572432|fr bdiffs=0(match) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>58620_SRR5572432|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +CGTCGAGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACGTCGA +>60476_SRR5572432|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +CGTCGAGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACGTCGA +>63048_SRR5572432|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +CGTCGAGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTCTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACGTCGA +>69944_SRR5572432|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +CGTCGAGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGGCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACGTCGA +>73213_SRR5572432|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +CGTCGAGTGAGTCATCGAGTCTTTGAACGCACATTGCGCCCGGTGGTAATCTGCCGGGCATGCCTGTTCGAGCGTCATTTCTACCCTCAGGCCCAGCCGCTTAGGCGGTTCGGGTGCTGGTGTTGGGGCATGCAGGCCACTGCAGCCTCTAAATTCATCGGCGTACTGGCGTACGTTGCCCTTTGCGTAGTGGATTTTTCTTTTCTCGCATCGGGCCGTGCCTCGTCCGGCCGCTAACTTTTTTTACTTTTTTAGTTTGACCTCGAATCAGGTAGGGTTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGACGTCGA +>74880_SRR5572432|fr bdiffs=0(match) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +TCGACGTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTAGTCCTACCTGATTTGAGGCCAGATGTCAAGAATATTAATCGTAAGATCAATGGATTAGAAAGCGGTCTTTAGTCCTGCAACGCGGCCATCCGAAGATGTCCTTAGCGAAATNCTTATTACGCCAAGTCAAACCATGTCGCGAGACAGATCCAGCTATTACTTTTAAGACGAGCCGACTTATCATCGACAAACGTCCAAATCCAAACCAAAGAAAGGTTTTAACCAATCTAGGGTTGAGGGTTTTCATGACACTCAAACAGGCATGCTCCTCGGAATACCAAGGAGCGCAAGGTGCGTTCAAAGACTCGATGACTCACTCGACG +>77272_SRR5572432|fr bdiffs=0(match) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACNATTTGTGACACACAGGCAGACGTGCCCTCNGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>80421_SRR5572432|fr bdiffs=0(match) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>88221_SRR5572432|fr bdiffs=0(match) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGATTCACTCGACG +>96939_SRR5572432|fr bdiffs=0(match) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>99283_SRR5572432|fr bdiffs=0(match) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGAGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>100926_SRR5572432|fr bdiffs=0(match) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +TCGACGTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTAGTCCTACCTGATTTGAGGTCAAGAAGTTCATTAAACATTGTCCGACAGGGGACGATTAGCAGCTGNACTAGTCAGAGAGCAATCGTCACAGTGTAGATAATTATCACACTAGTGACGGGACTGCAAACGGTTCCGCTAATGCATTTTAGGAGAGCCGACCTCTATTAATAAAGGCCCGCAAACTCCCAACATCCAAGCCCCATCGCAGCTCGCAAAAGCTGGAGGGGTTGAGAATTTAATGACACTCAAACAGGCATGCTCCTCGGAATACCAAGGAGCGCAAGGTGCGTTCAAAGACTCGATGACTGACTCGACG +>107559_SRR5572432|fr bdiffs=0(match) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>18259_SRR5572433|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GTCGCGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTTTCCAAATGCTCTCGATGACTCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGTCGC +>18907_SRR5572433|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GTCGCGTGAGTCATCGAATCTTTGAACGCATATTGCGCTCTTTGGTATTCCGAAGAGCATGCTTGTTTGAGTATCAGTAAACACCTCAAAGCTTTTGATTTTTTTTGAAAGCTTTGGACTTGAGCAATCCCAACACCAGTCTTTTTTAGATCGGAGGCGGGTTGCTTGAAATGCAGGTGCAGCTGGACATTCTCCTGAGCTAAAAGCATATTCATTTAGTCCCGTCAAACGGATTATTACTTTCGCTGCAGCTAACATAAAGGGAGTTTGACCGTATTGGCTGACTGATGCAGGATTTCACAGGGGTCGGCAACGACGCTTGTTAAACTCGATCTCAAATCAAGTAAGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAGTCGC +>33225_SRR5572433|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +GCGACTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTAGTCCTACCTGATTTGAGATCAGATCATAAAGGTATTGTCCAAGTCAATGGACTATTAGAAGCGGTTCATCTGCATTTACCTTGGCCACCTTTTACNGTGTCCTCAGCGATTGATAATTTATCACGCCGAGTGGAACCAAGCATAACACTGAGATCCAGCTAATGAACAAAGAGGAGCAGGTGTGAAGCTGCAAAAGACCTCCAATACCAAAGTGAAACCAATTGAGTTAACAAAAGATTTACTTTGAAGAATTCATGATACTCAAACAGGCATGCTCCAAGGAATACCAAGGAGCGCAAGGTGCGTTCAAAGATTCGATGATTCACGCGAC +>36440_SRR5572433|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +GCGACTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTAGTCCTACCTGATTTGAGATCAGATCATAAAGGTATTGTCCAAGTCAATGGACTATTAGAAGCGGTTCATCTGCATTTACCTTGGCCACCTTTTACAGTGTCCTCAGCGATAGATAATTTATCACGCCGAGTGGAACCAAGCATAACACTGAGATCCAGCTAATGAACAAAGAGGAGCAGGTGTGAAGCTGCAAAAGACCTCCAATACCAAAGTGAAACCAATTGAGTTAACAAAAGATTTACTTTGAAGAATTCATGATACTCAAACAGGCATGCTCCAAGGAATACCAAGGAGCGCAAGGTGCGTTCAAAGATTCGATGACTCACGCGAC +>42477_SRR5572433|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GTCGCGTGAGTCATCGAATCTTTGAACGCATATTGCGCTCTCTGGTATTCCGGAAAGCATGCTTGTTTGAGTATCAGTAAACACCTCAACTCCCTCTTTTCTTTNTTGAAATGAGGGNGCTGGACTTGAGTGATCCCAACACTTTTCTTCACTGAAAAGTGGCGGGTCACTTGAAATGCAGGTGCAGCTGGACTTTTCTCTGAGCTATAAGCATATCTATTTAGTCTGCCTAAAAAACAGATTATTACCTTTGCTGCAGCTAACATAAAGGAGATGAGTTCTTGTGCTGACTGATGCAGGATTCACAGAGACAGCTTCGCGGCTGACTTCGTAAACTCGATCTCAAATCAAGTAAGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAGTCGC +>50746_SRR5572433|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GTCGCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTATTCGAGCGTCATTATCACCCCTCAAGCCTAGCTTGGTGTTGAGGCCTGCTGTCAAGGCAGCCTCTAAAATCAGTGGCAGTGCTGTCAGGCTCTAAGCGTAGTAAAATAAATCGCTATAGGGTCCTGGTGGATACTCGTCAGAACCCCCCCATTTTTTAATGATTGACCTCGGATTAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAGTCGC +>10088_SRR5572434|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +CGTCGAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCGGTAGTAATCTGCCGGGCATGCCTGTCCGAGCGTCATTTCACCCCTCGAACCACTATTCGCGTAGCTGGATCGGTGTTGGGGCACTACGGTGCAATACCCGTAGGCCCCGAAATGAAGTGGCGGTCCCGCCACGGCGCCCCCATGCGTAGTAACTTTCAGTCTCGCATCGGGTCCCGGCGGAGGCCTGCCGTCAAGCCTATTTTATAATATCTTTACAGATGGTTTGACCTCGGATCAGGTAGGGTTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGACGTCGA +>10939_SRR5572434|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +CGTCGAGTGAATCATCGAATCTTTGAACGCATATTGCGCTCTCTGGTATTCCGGAGAGCATGCTTGTTTGAGTATCAGTAAACACCTCAACTCCCTTTTCTTTTTTGAAATGGGAGCTGGACTTGAGTGATCCCAACACTTTTCTCACTGGAAAGTGGCGGGTCACTTGAAATGCAGGTGCAGCTGGACTTTTCTCTGAGCTATAAGCATATCTATTTAGTCTGCCTAAAAAACAGATTATTACCTTTGCTGCAGCTAACATAAAGGAGATGAGTTCCTGTGCTGACCGATGCAGGATTCACAGAGACAGCTTCGCGGCTGACTTTGTAAACTCGATCTCAAATCAAGTAAGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGACGTCGA +>19705_SRR5572434|fr bdiffs=0(match) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +TCGACGTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTATCCCTACCTGATCCGAGGTCAAACTGAGAATCAAAAGACGTGTGCGACAGCCCTGCGGCCTGGTTCTTGTGCGAACAATCTGCTACGCGCAAGACCAACCGCCGGACGCCGATGCTTTTCAGGCGAGTCCACTGCGCAACGCAGGGACAGACGCCCAACACCAAGCTGTGCTTGAGAGGTGTAATGACGCTCGAACAGGTATGCCCTTCGGAATGCCAAAGGGCGCAATGTGCGTTCAAAGATTCGATGACTCACTCGACG +>25963_SRR5572434|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +CGTCGAGTGAGTCATCGAATCTTTGAACGCACGTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTTTCACCACTCAAGCCCCCGGCTTGGTGTTGGACGGTTTGGTTTAGGTACCCCTAGACCCCTCCTAAGGACAATGACGGCGGCTGTTGAACCCCCGGTACACTGAGCTTCATAACTGAGCACGTATCGGATCTAGGGTTTGGCGGCACCCGGTCTATACCTTTTTTGCTTACAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGACGTCGA +>60502_SRR5572434|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +CGTCGAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTATAACCCTCAAGCCTAGCTTGGTGTTGGAGCATGCTATCTAGCAGCTCTTAAAATCAGTGGCAGTGCCTTCCGGCTCTAAGCGTAGTAATTCTTCTCGCTATAGAAACCGGTTGGTTCGCGCCAGAACCCCCAATTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGACGTCGA +>5314_SRR5572435|fr bdiffs=0(match) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +ACTGCTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCNCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCNCCACCNCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCAATGATTCACACTGC +>21163_SRR5572435|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GCAGTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGCAGT +>23380_SRR5572435|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GCAGTGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGCAGT +>28473_SRR5572435|fr bdiffs=0(match) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +ACTGCTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACACTGC +>44904_SRR5572435|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GCAGTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGGGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGCAGT +>83509_SRR5572435|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GCAGTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTATCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCNCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGCAGT +>10044_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CATGCGGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAGAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>27083_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CATGCGGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>30856_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCTAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACCGCATG +>31515_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CATGCGGTGAGTCATCGAGTCTTTGAACACAAGTTGCGCCCCAAGCCTTCTGGCTGGGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>39390_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCCGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGNCGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGANGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCNCAGCCAGAAGGCTTGNNGCGCAACNTGCGTTCAAAGACTCGATAATTCACCGCATG +>43887_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGCGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGATTCGATGACTCACCGCATG +>49165_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACCGCATG +>56974_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGNTCCCGCCTGACCNGGGGTCGCGTTAAAGACTTTGNGNNATNGAGAGCATTTGGACAGGAGCGACCGACANAANNNCNAGGCTTTGTTTCACCACCGCANGTCGAAACGCTCNAANGCGTCCTCAGCTNAGATNTAGGCCAACCGCGTGCGGNNACACACGGGAGACCAGCTTCCGTCCAGTANCCGCAAAGGATGGGGACGACGNTTTGTGACAGCCAGGCGGNCGTGCCCTCANCCAGAAGGNTTNGGGCGCANCTTGCATTCAAGGACTCGATGACTCACCGCATG +>59309_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CATGCGGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>60649_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTACTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACCGCATG +>63598_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACTTGGGGTCGCGTTAAAGACTTTGNGTCATCGAGAGCATTTGGACAGGNGCGACCGACAAAATGACGNGGCTTTGTTTCACCACNGCATGTCGAGACGCTCCAAGGTGTCCNCAGCNCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGAACAGCNTCCGNCNGGTATCCNCAAAGGAGGGGGACGACGANTTGTGANACCCAGGCAGACGTGCCCTCAGCCAGAAAGCTTGGGGCGCAACNTGCGTTCAAAGACTCGATGATTCACCGCATG +>78636_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGATTCGATGACTCACCGCATG +>79692_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CGCATGTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTAGTCCTACCTGATTTGAGATCAGATCATAAAGGTATTGTCCAAGTCAATGGACTATTGGAAGCGGTTCATCTGCATTTACCTTGGCCACCTTTTACAGTGTCCTCAGCGATAGATAATTTATCACGCCGAGTGGAACCAAGCATAACACTGAGATCCAGCTAATGCACAAAGAGGAGCAGGTGTGAAGCTGCAAAAGACCTCCGATACCAAAGTGAAACCAATTGAATTAACAAAAGATTTACTTTGAAGAATTCATGATACTCAAACAGGCATGCTCCAAGGAATACCAAGGAGCGCAAGGTGCGTTCAAAGACTCGATGACTCACCGCATG +>90730_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CATGCGGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAAGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCCCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>118563_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CATGCGGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>133834_SRR5572436|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CATGCGGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGNCTGAGNGCACGTCTGCCTGGGTTTCACAAATCGTCGTCCCCATCCTTTTCGGATACCGGACGGAAGCTGNNCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGANATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATNACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>5749_SRR5572437|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +ACAGTCTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTAGTCTTACTTGATTTGAGATCGAGTTTACAAAGACAGCCGAAGCGGTCTTTGTGAATCCTGCATCAGTCAGCACAAGAACTAATCTCCTTTATGTTAGCTGCAGCAAAGGTAATAATCTGTTTTTGAGGCAGACTAAATAGATATGCTTATAGCTCAGAGAAAAGTCCAGCTGCACCTGCATTTCAAGTAACCCGCCACTTTATGAATAAAGCGTTGGGATCACTCAAGTCCAGCTCCATTTCAAGAAGAAAGGGAGTTGAGGTGTTTACTGATACTCAAACAAGCATGCTCTCCGGAATACCAGAGAGCGCAATATGTGTTCAAAGATTCGATGACTCACACAGTC +>6609_SRR5572437|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +GGACTGTGTGAGTCATCGAATCTTNAAAGCACATTGCGCCCCTTGNAATTCCNTGGGGCATGCNTGTTCNAGCGTCATTTGTNCCTTCAAGCTCTGCTTGNTGTTGGGNGTTTNTCTCGCCTTGGNGTGTAGACTCGCCNCAAACCAATNGGCGGCCGGCGTATTGATTTCGGNGCGCAGTACATCTCGCGCTTTGCACTCATAACGACGACGTCCAAAAGTACATTTTTACACTCTTGACCTCGGATCAGGTAGGGATACCCGCTGAATTTAAGCATANCAATAAGCGGAGGAGACTGTG +>23055_SRR5572437|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +ACAGTCTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTAGTCCTACCTGATTTGAGGTCAAATTCATCAAAAGGTTTTATCTTTGTTGGAAGCAGTTCATGCAGCATACCAAAGGCATAGATAATTTATCACACCTATAGTAATAGCCTCAGATACTACTAATACATTTCAGGGGAGCTGACCTCAAGAAAAGCCTGCAAACAACCCCCCACATCCAAGCCACCCAAAAAAAATCAAGTGGTTGAGAAGTTAAATGACACTCAAACAGGCATGCTCCTCGGAATACCAAGGAGCGCAAGATGCGTTCAAAGATTCGATGACTCACACAGTC +>27631_SRR5572437|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +ACAGTCTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTATCCCTACCTGATCCGAAGTCAAACTCTTGATGGGTTATGGGGTTCAACGGCATGATACCAGCAAGGTCTTGCGCCAGGCGAGAGCTTTTTAGGCTTACTACGCTTTGGCGACCTGTGGATCAGCACAATGGAATTCGCCCAGGAGGAACCAGAGGTACCTCCACGAGCAACACCGAGGCTCCTGTGGCCGGTTAAAGCCAGAGGACTCGAGGGGTTTGATGACGTTCGAACAGGCATGCCTTACGGACTACCATAAGGCGCAATGTGCGTTCAAAGATTCGATGATTCACACAGTC +>30780_SRR5572437|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +ACAGTCTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTATCCCTACCTGATCCGAGGTCAAAGTAAAAAAAGGCTTGTGGAAGGGGGTCTGTTGAAATCGCTTGCAATGTGCTGCGCACGAAGCCAACATACCGGCTGCCAATGAATTTAAGGCGAGTCCACACGCTGAGGCGGAACAAACACCCAACACCAAGCAGAGCTTGAAGGTTTAAATGACGCTCGAACAGGCATGCCCAACGGAATACCGAAGGGCGCAATGTGCGTTCAAAGATTCGATGACTCACACAGTC +>33944_SRR5572437|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +ACAGTCTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTATCCCTACCTGATCCGAGGTCAACCATAGAAGGGGGGTTCTGGCAGGCCACCGCCTAGCACCAGAGCGAGGATTGTACTACGCTTGGAGCCAGACGCTGCCGCCACTGTCTTTAGGGCCGGCCGCGAGCGGGCGGGGCCCAACACCAAGCAGAGCTTGAGGGTTGAAATGACGCTCGAACAGGCATGCCCTGCGGAATACCACAGGGCGCAATGTGCGTTCAAAGATTCGATGACTCACACAGTC +>36405_SRR5572437|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GACTGTGTGAGTCATCGAATCTTTGAACGCATATTGCGCTCTTTGGTATTCCGAAGAGCATGCTTGTTTGAGTATCAGTAAACACCTCAAAGCCTTTTTTCTTTTTTGAAGAAAGGCTTTGGACTTGAGCAATCCCAACACCAATCTTTTAAAGAGAGGGGGCGGGTTGCTTGAAATGCAGGTGCAGCTGGACATTCTCCTGAGCTAAAAGCATATTCATTTAGTCCCGTCAAACGGATTATTACTTTTGCTGCAGCTAATATAAAGGGAGTTTGACTATTTTGGCTGACTGATGCAGGATTTTCACAAGAGTCTTCAAAACCTCTTGTTAAACTCGATCTCAAATCAAGTAAGACTACTCGCTGAACTTAAGCATATCAATAAGCGGAGGAGACTGT +>37680_SRR5572437|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CCGATCCACAGTCTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTATCCCTACCTAATCCGAGGTCAATCATTAAAAAAAATGGGGGGTTCTGACGAGTATCCACCAGGACCCTATAGCGATTTATTTTACTACGCTTAGAGCCTGACAGCACTGCCACTGATTTTAGAGGCTGCCTTGACAGCAGGCCTCAACACCAAGCTAGGCTTGAGGGGTGATAATGACGCTCGAATAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGATTCAATGACTCACACAGTC +>45410_SRR5572437|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +TGCCGATCTGACTGTGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCCGTGGTATTCCGCGGGGCATGTCTGTTCGAGCGTCAGTACAACACTCAAGCTCTGCTTGGTATTGGGCCTTGCCGGCAACGGCAGGCCTTAAAATCAGTGGCGGCGCCATCTGGCTCTAAGCGTAGTAATACCCCTCGCTATAGAGTTCCGGTGGATGCTGGCTAGCAACCCCCAACTTTCTAAGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAGACTGT +>56304_SRR5572437|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GACTGTGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCATGGGGCATGCCTGTTCGAGCGTCATTTGTACCTTCAAGCCTTGCTTGGTGTTGGGTGTTTGTCTCGCCTTTGCGTGTAGACTCGCCTCAAAACAATTGGCAGCCGGCGTATTGATTTCGGAGCGCAGTACATCTCGCGCTTTGCACTCATAACGACGACGTCCAAAAGTACATTTTACACTCTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAGACTGT +>58773_SRR5572437|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +GACTGTGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTCGGTATTCCGTTGGGCATGCCTGTTCGAGCGTCATTTAAACCTTCAAGCTCTGCTTGGTGTTGGGTGTTTGTTCCGCCTCAGCGTGTGGACTCGCCTTAAATTCATTGGCAGCCGGTATGTTGGCTTCGTGCGCAGCACATTGCAAGCGATTTCAACAGACCCCCTTCCACAAGCCTTTTTTTACTTAGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAGACTGT +>65449_SRR5572437|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +CCCGATCTACAGTCTCCTCCGCTTATTGATATGCTTAAATTCAGCGGGTATCCCTACCTAATCCGAGGTCAATCATTAAAAAAATGGGGGGGTTCTGACGAGTATCCACCAGGACCCTATAGCGATTTATTTTACTACGCTTAGAGCCTGACAGCACTGCCACTGATTTTAGAGGCTGCCTTGNCAGCAGGCCTCAACACCAAGCTAGGCTTGAGGGGTGATAATGACGCTCGAATAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGATTCGATGACTCACACAGTC +>26515_SRR5572438|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAGTCATCGAATCTTTGAACGCACATTGCACTCCTTGGTATTCCGAGGAGTATGCCTGTTTCAGTATCATGAGCACTCTCACACCTAACCTTTGGGTTATGATGTGGAATTGGGATGCGCCGATTTTTACAAGTCGGCACTCCTAAAATGTAGCTCTTGGCTGTTTCCTACTACAGCAGTTTGGCCTAATAGTTTTGACTTTTGTCAAATCTTTGGCTACATTTGCTTCTGGAAATCAGTCTTGATAATACAGAAAACTCATTTCAAACTTTGATCTGAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGATAGCT +>37564_SRR5572438|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +AGCTATCCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTAGTCCTACCTGATTTGAGATCAGATCATAAAGGTATTGTCCAAGTCAATGGACTATTAGAAGCGGTTCATCTGCATTTACCTTGGCCACCTTTTACAGTATCCTNAGCGATNGATAATTTATCACGCCGAGTGGANCCAAGCATAACACTGANATCCAGCTAATGAACAAAGAGNAGCAGGTGTGAAGCTGCAAAAGACCTCCAATACCAAAGTGAAACCAATTGNGTTAACAAAAGATTTACTNTGAAGAATACATANTACTCAAACAGGCGTGCTCCAAGGTATACCGAAGAGCGCAAGGTGCGTTCGAAGATTCGATGACTCACAGCTA +>54859_SRR5572438|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGATTCGATGATTCACAGCTA +>64529_SRR5572438|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCTTGGAGCATGCCTGTTTGAGTATCATGAATTCTTCAAAGTAAATCATTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACGCCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATGATCTGATCTCAAATCAGGTAGGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGATAGCT +>95460_SRR5572438|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGACTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCCCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>97519_SRR5572438|bfr bdiffs=1000(noMatch) fpdiffs=1000(noMatch) rpdiffs=1000(noMatch) +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACATGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGAGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGATTCGATGACTCACAGCTA +>100491_SRR5572438|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAGTCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCTTGGAGCATGCCTGTTTGAGTATCATGAATTCTTCAAAGTAGATCTTTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATGATCTGATCTCAAATCAGGTAGGACTTCCCGCTGAACTTAAGCATATCAATAAGCGGAGGATAGCT +>103686_SRR5572438|r bdiffs=0(match) fpdiffs=0(match) rpdiffs=1000(noMatch) +TAGCTGTGAGTCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCTTGGAGCATGCCTGTTTGAGTATCATGAATTCTTCAAAGTAAATCATTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGGCTTGGACAATACCTTTATGATCTGATCTCAAATCAGGTAGGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGATAGCT \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test_01_found.tabular Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,38 @@ +7808_SRR5572429|bfr 375 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-309 LSU: 310-375 Broken or partial sequence, only partial 5.8S! +17841_SRR5572429|r 323 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-257 LSU: 258-323 Broken or partial sequence, only partial 5.8S! +10297_SRR5572430|r 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +70284_SRR5572430|bfr 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +151248_SRR5572430|r 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +155395_SRR5572430|r 377 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-312 LSU: 313-377 Broken or partial sequence, only partial 5.8S! +53558_SRR5572432|fr 497 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-431 LSU: 432-497 Broken or partial sequence, only partial 5.8S! +73213_SRR5572432|r 320 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-254 LSU: 255-320 Broken or partial sequence, only partial 5.8S! +74880_SRR5572432|fr 365 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-299 LSU: 300-365 Broken or partial sequence, only partial 5.8S! +100926_SRR5572432|fr 358 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-292 LSU: 293-358 Broken or partial sequence, only partial 5.8S! +18907_SRR5572433|r 391 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-326 LSU: 327-391 Broken or partial sequence, only partial 5.8S! +33225_SRR5572433|bfr 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +36440_SRR5572433|bfr 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +42477_SRR5572433|r 399 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-334 LSU: 335-399 Broken or partial sequence, only partial 5.8S! +50746_SRR5572433|r 290 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-225 LSU: 226-290 Broken or partial sequence, only partial 5.8S! +10088_SRR5572434|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-263 LSU: 264-329 Broken or partial sequence, only partial 5.8S! +10939_SRR5572434|r 396 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-330 LSU: 331-396 Broken or partial sequence, only partial 5.8S! +19705_SRR5572434|fr 303 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-237 LSU: 238-303 Broken or partial sequence, only partial 5.8S! +25963_SRR5572434|r 318 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-252 LSU: 253-318 Broken or partial sequence, only partial 5.8S! +60502_SRR5572434|r 287 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-221 LSU: 222-287 Broken or partial sequence, only partial 5.8S! +79692_SRR5572436|bfr 374 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-308 LSU: 309-374 Broken or partial sequence, only partial 5.8S! +5749_SRR5572437|bfr 388 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-322 LSU: 323-388 Broken or partial sequence, only partial 5.8S! +6609_SRR5572437|bfr 301 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-234 LSU: 235-301 Broken or partial sequence, only partial 5.8S! +23055_SRR5572437|bfr 334 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-268 LSU: 269-334 Broken or partial sequence, only partial 5.8S! +27631_SRR5572437|bfr 338 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-272 LSU: 273-338 Broken or partial sequence, only partial 5.8S! +30780_SRR5572437|bfr 293 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-227 LSU: 228-293 Broken or partial sequence, only partial 5.8S! +33944_SRR5572437|bfr 286 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-220 LSU: 221-286 Broken or partial sequence, only partial 5.8S! +36405_SRR5572437|r 398 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-332 LSU: 333-398 Broken or partial sequence, only partial 5.8S! +37680_SRR5572437|bfr 300 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-227 LSU: 228-300 Broken or partial sequence, only partial 5.8S! +45410_SRR5572437|bfr 300 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 90-234 LSU: 235-300 Broken or partial sequence, only partial 5.8S! +56304_SRR5572437|r 299 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-233 LSU: 234-299 Broken or partial sequence, only partial 5.8S! +58773_SRR5572437|r 293 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-227 LSU: 228-293 Broken or partial sequence, only partial 5.8S! +65449_SRR5572437|bfr 301 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-227 LSU: 228-301 Broken or partial sequence, only partial 5.8S! +26515_SRR5572438|r 340 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-275 LSU: 276-340 Broken or partial sequence, only partial 5.8S! +37564_SRR5572438|bfr 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +64529_SRR5572438|r 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +100491_SRR5572438|r 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +103686_SRR5572438|r 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S!
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test_01_its2.fasta Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,76 @@ +>7808_SRR5572429|bfr|F|ITS2 Extracted ITS2 sequence 80-309 (230 bp) +AATTCTTCAAAGTAAATCTTTTGTTAATTCAATTGGTTTCACTTTGGTATTGGAGGTCCTTTGCAGCTTCACACCTGCTCCTCTTTGTGCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCTCTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCCAATAGTCCATTGGCTTGGACAATACCTTTATG +>17841_SRR5572429|r|F|ITS2 Extracted ITS2 sequence 81-257 (177 bp) +ACACCCCACATTCATAATTTTGTTGTGAATGGAAATGAGAGTTTCGGCTTTATTGCTGAATTCTTTAAAATTATTAGGCCTGAACTATTGTTCTTTCTGCCTGAACATTTTTTTAATATAAAGGAATGCTCTAGTAAAAAGACTATCTCTGGGGCCTCCCAAATAAATCATTCTTAA +>10297_SRR5572430|r|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAAATCATTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTGTGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATG +>70284_SRR5572430|bfr|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAAATCATTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATG +>151248_SRR5572430|r|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAAATCTTTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCTTTGACTTGGACAATACCTTTATG +>155395_SRR5572430|r|F|ITS2 Extracted ITS2 sequence 79-312 (234 bp) +AAATTCTCAAAGTAAACCTTTTGTTAATTCAACTGGTCTTTGCTTTGGATTTGGAGGCCTCTGCAGATGTCACAGTCTGCTCCTCTTAAATAAATTAGCTGGATCTCAGTATACACTTGGTTCCACTCGGCGTGATAAGTATCACTCGCTGAGGACACTGTTAAAAAGGTGGCCAGGCAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAAATTAAAAATTTATG +>53558_SRR5572432|fr|F|ITS2 Extracted ITS2 sequence 81-431 (351 bp) +TCAACCACACCTCGGCGTCATCTCTTCATTGCGACTGCGCATCTGGAGATTGAGTCGGTCACCGAGTTAACTCTTTGGTGTCCCTTCAAATGTGGCAAGGCTTCAGTGTATTGCTCTTCGCGGACAGTATTCCGATTGTACTCGCTAGCGACGTGGATACTCGATCTCTTTTGGTCTTGAATCCAATACGTGGTTAGTGTTTAGGAAAGGAGTATTGCGCCGTGTGCACCGTCTGTGAGTCCTCTCTTTTGAGGACAAACAGGCTGATTCGCTTGTTTTTGATTTGCGTTTGTGCGCCTCGTGTGTACAGACTGCCGATCAAATCCACACACTTGTGTGTGCTGTTTTCGA +>73213_SRR5572432|r|F|ITS2 Extracted ITS2 sequence 81-254 (174 bp) +CTACCCTCAGGCCCAGCCGCTTAGGCGGTTCGGGTGCTGGTGTTGGGGCATGCAGGCCACTGCAGCCTCTAAATTCATCGGCGTACTGGCGTACGTTGCCCTTTGCGTAGTGGATTTTTCTTTTCTCGCATCGGGCCGTGCCTCGTCCGGCCGCTAACTTTTTTTACTTTTTTA +>74880_SRR5572432|fr|F|ITS2 Extracted ITS2 sequence 81-299 (219 bp) +AAACCCTCAACCCTAGATTGGTTAAAACCTTTCTTTGGTTTGGATTTGGACGTTTGTCGATGATAAGTCGGCTCGTCTTAAAAGTAATAGCTGGATCTGTCTCGCGACATGGTTTGACTTGGCGTAATAAGNATTTCGCTAAGGACATCTTCGGATGGCCGCGTTGCAGGACTAAAGACCGCTTTCTAATCCATTGATCTTACGATTAATATTCTTGAC +>100926_SRR5572432|fr|F|ITS2 Extracted ITS2 sequence 81-292 (212 bp) +AATTCTCAACCCCTCCAGCTTTTGCGAGCTGCGATGGGGCTTGGATGTTGGGAGTTTGCGGGCCTTTATTAATAGAGGTCGGCTCTCCTAAAATGCATTAGCGGAACCGTTTGCAGTCCCGTCACTAGTGTGATAATTATCTACACTGTGACGATTGCTCTCTGACTAGTNCAGCTGCTAATCGTCCCCTGTCGGACAATGTTTAATGAACT +>18907_SRR5572433|r|F|ITS2 Extracted ITS2 sequence 80-326 (247 bp) +AACACCTCAAAGCTTTTGATTTTTTTTGAAAGCTTTGGACTTGAGCAATCCCAACACCAGTCTTTTTTAGATCGGAGGCGGGTTGCTTGAAATGCAGGTGCAGCTGGACATTCTCCTGAGCTAAAAGCATATTCATTTAGTCCCGTCAAACGGATTATTACTTTCGCTGCAGCTAACATAAAGGGAGTTTGACCGTATTGGCTGACTGATGCAGGATTTCACAGGGGTCGGCAACGACGCTTGTTAA +>33225_SRR5572433|bfr|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAAATCTTTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCAATCGCTGAGGACACNGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATG +>36440_SRR5572433|bfr|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAAATCTTTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATG +>42477_SRR5572433|r|F|ITS2 Extracted ITS2 sequence 80-334 (255 bp) +AACACCTCAACTCCCTCTTTTCTTTNTTGAAATGAGGGNGCTGGACTTGAGTGATCCCAACACTTTTCTTCACTGAAAAGTGGCGGGTCACTTGAAATGCAGGTGCAGCTGGACTTTTCTCTGAGCTATAAGCATATCTATTTAGTCTGCCTAAAAAACAGATTATTACCTTTGCTGCAGCTAACATAAAGGAGATGAGTTCTTGTGCTGACTGATGCAGGATTCACAGAGACAGCTTCGCGGCTGACTTCGTAA +>50746_SRR5572433|r|F|ITS2 Extracted ITS2 sequence 80-225 (146 bp) +TCACCCCTCAAGCCTAGCTTGGTGTTGAGGCCTGCTGTCAAGGCAGCCTCTAAAATCAGTGGCAGTGCTGTCAGGCTCTAAGCGTAGTAAAATAAATCGCTATAGGGTCCTGGTGGATACTCGTCAGAACCCCCCCATTTTTTAAT +>10088_SRR5572434|r|F|ITS2 Extracted ITS2 sequence 81-263 (183 bp) +CACCCCTCGAACCACTATTCGCGTAGCTGGATCGGTGTTGGGGCACTACGGTGCAATACCCGTAGGCCCCGAAATGAAGTGGCGGTCCCGCCACGGCGCCCCCATGCGTAGTAACTTTCAGTCTCGCATCGGGTCCCGGCGGAGGCCTGCCGTCAAGCCTATTTTATAATATCTTTACAGATG +>10939_SRR5572434|r|F|ITS2 Extracted ITS2 sequence 81-330 (250 bp) +AACACCTCAACTCCCTTTTCTTTTTTGAAATGGGAGCTGGACTTGAGTGATCCCAACACTTTTCTCACTGGAAAGTGGCGGGTCACTTGAAATGCAGGTGCAGCTGGACTTTTCTCTGAGCTATAAGCATATCTATTTAGTCTGCCTAAAAAACAGATTATTACCTTTGCTGCAGCTAACATAAAGGAGATGAGTTCCTGTGCTGACCGATGCAGGATTCACAGAGACAGCTTCGCGGCTGACTTTGTAA +>19705_SRR5572434|fr|F|ITS2 Extracted ITS2 sequence 81-237 (157 bp) +CACCTCTCAAGCACAGCTTGGTGTTGGGCGTCTGTCCCTGCGTTGCGCAGTGGACTCGCCTGAAAAGCATCGGCGTCCGGCGGTTGGTCTTGCGCGTAGCAGATTGTTCGCACAAGAACCAGGCCGCAGGGCTGTCGCACACGTCTTTTGATTCTCA +>25963_SRR5572434|r|F|ITS2 Extracted ITS2 sequence 81-252 (172 bp) +TCACCACTCAAGCCCCCGGCTTGGTGTTGGACGGTTTGGTTTAGGTACCCCTAGACCCCTCCTAAGGACAATGACGGCGGCTGTTGAACCCCCGGTACACTGAGCTTCATAACTGAGCACGTATCGGATCTAGGGTTTGGCGGCACCCGGTCTATACCTTTTTTGCTTACAA +>60502_SRR5572434|r|F|ITS2 Extracted ITS2 sequence 81-221 (141 bp) +TAACCCTCAAGCCTAGCTTGGTGTTGGAGCATGCTATCTAGCAGCTCTTAAAATCAGTGGCAGTGCCTTCCGGCTCTAAGCGTAGTAATTCTTCTCGCTATAGAAACCGGTTGGTTCGCGCCAGAACCCCCAATTTTCTAT +>79692_SRR5572436|bfr|F|ITS2 Extracted ITS2 sequence 80-308 (229 bp) +AATTCTTCAAAGTAAATCTTTTGTTAATTCAATTGGTTTCACTTTGGTATCGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTGCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCCAATAGTCCATTGACTTGGACAATACCTTTATG +>5749_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 81-322 (242 bp) +AACACCTCAACTCCCTTTCTTCTTGAAATGGAGCTGGACTTGAGTGATCCCAACGCTTTATTCATAAAGTGGCGGGTTACTTGAAATGCAGGTGCAGCTGGACTTTTCTCTGAGCTATAAGCATATCTATTTAGTCTGCCTCAAAAACAGATTATTACCTTTGCTGCAGCTAACATAAAGGAGATTAGTTCTTGTGCTGACTGATGCAGGATTCACAAAGACCGCTTCGGCTGTCTTTGTAA +>6609_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 81-234 (154 bp) +GTNCCTTCAAGCTCTGCTTGNTGTTGGGNGTTTNTCTCGCCTTGGNGTGTAGACTCGCCNCAAACCAATNGGCGGCCGGCGTATTGATTTCGGNGCGCAGTACATCTCGCGCTTTGCACTCATAACGACGACGTCCAAAAGTACATTTTTACAC +>23055_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 81-268 (188 bp) +AACTTCTCAACCACTTGATTTTTTTTGGGTGGCTTGGATGTGGGGGGTTGTTTGCAGGCTTTTCTTGAGGTCAGCTCCCCTGAAATGTATTAGTAGTATCTGAGGCTATTACTATAGGTGTGATAAATTATCTATGCCTTTGGTATGCTGCATGAACTGCTTCCAACAAAGATAAAACCTTTTGATGA +>27631_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 80-272 (193 bp) +AAACCCCTCGAGTCCTCTGGCTTTAACCGGCCACAGGAGCCTCGGTGTTGCTCGTGGAGGTACCTCTGGTTCCTCCTGGGCGAATTCCATTGTGCTGATCCACAGGTCGCCAAAGCGTAGTAAGCCTAAAAAGCTCTCGCCTGGCGCAAGACCTTGCTGGTATCATGCCGTTGAACCCCATAACCCATCAAGA +>30780_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 81-227 (147 bp) +AAACCTTCAAGCTCTGCTTGGTGTTGGGTGTTTGTTCCGCCTCAGCGTGTGGACTCGCCTTAAATTCATTGGCAGCCGGTATGTTGGCTTCGTGCGCAGCACATTGCAAGCGATTTCAACAGACCCCCTTCCACAAGCCTTTTTTTA +>33944_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 81-220 (140 bp) +CAACCCTCAAGCTCTGCTTGGTGTTGGGCCCCGCCCGCTCGCGGCCGGCCCTAAAGACAGTGGCGGCAGCGTCTGGCTCCAAGCGTAGTACAATCCTCGCTCTGGTGCTAGGCGGTGGCCTGCCAGAACCCCCCTTCTAT +>36405_SRR5572437|r|F|ITS2 Extracted ITS2 sequence 81-332 (252 bp) +AACACCTCAAAGCCTTTTTTCTTTTTTGAAGAAAGGCTTTGGACTTGAGCAATCCCAACACCAATCTTTTAAAGAGAGGGGGCGGGTTGCTTGAAATGCAGGTGCAGCTGGACATTCTCCTGAGCTAAAAGCATATTCATTTAGTCCCGTCAAACGGATTATTACTTTTGCTGCAGCTAATATAAAGGGAGTTTGACTATTTTGGCTGACTGATGCAGGATTTTCACAAGAGTCTTCAAAACCTCTTGTTAA +>37680_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 81-227 (147 bp) +TCACCCCTCAAGCCTAGCTTGGTGTTGAGGCCTGCTGTCAAGGCAGCCTCTAAAATCAGTGGCAGTGCTGTCAGGCTCTAAGCGTAGTAAAATAAATCGCTATAGGGTCCTGGTGGATACTCGTCAGAACCCCCCATTTTTTTTAAT +>45410_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 90-234 (145 bp) +CAACACTCAAGCTCTGCTTGGTATTGGGCCTTGCCGGCAACGGCAGGCCTTAAAATCAGTGGCGGCGCCATCTGGCTCTAAGCGTAGTAATACCCCTCGCTATAGAGTTCCGGTGGATGCTGGCTAGCAACCCCCAACTTTCTAA +>56304_SRR5572437|r|F|ITS2 Extracted ITS2 sequence 81-233 (153 bp) +GTACCTTCAAGCCTTGCTTGGTGTTGGGTGTTTGTCTCGCCTTTGCGTGTAGACTCGCCTCAAAACAATTGGCAGCCGGCGTATTGATTTCGGAGCGCAGTACATCTCGCGCTTTGCACTCATAACGACGACGTCCAAAAGTACATTTTACAC +>58773_SRR5572437|r|F|ITS2 Extracted ITS2 sequence 81-227 (147 bp) +AAACCTTCAAGCTCTGCTTGGTGTTGGGTGTTTGTTCCGCCTCAGCGTGTGGACTCGCCTTAAATTCATTGGCAGCCGGTATGTTGGCTTCGTGCGCAGCACATTGCAAGCGATTTCAACAGACCCCCTTCCACAAGCCTTTTTTTA +>65449_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 81-227 (147 bp) +TCACCCCTCAAGCCTAGCTTGGTGTTGAGGCCTGCTGNCAAGGCAGCCTCTAAAATCAGTGGCAGTGCTGTCAGGCTCTAAGCGTAGTAAAATAAATCGCTATAGGGTCCTGGTGGATACTCGTCAGAACCCCCCCATTTTTTTAAT +>26515_SRR5572438|r|F|ITS2 Extracted ITS2 sequence 80-275 (196 bp) +GCACTCTCACACCTAACCTTTGGGTTATGATGTGGAATTGGGATGCGCCGATTTTTACAAGTCGGCACTCCTAAAATGTAGCTCTTGGCTGTTTCCTACTACAGCAGTTTGGCCTAATAGTTTTGACTTTTGTCAAATCTTTGGCTACATTTGCTTCTGGAAATCAGTCTTGATAATACAGAAAACTCATTTCAAA +>37564_SRR5572438|bfr|F|ITS2 Extracted ITS2 sequence 80-307 (228 bp) +ATTCTTCANAGTAAATCTTTTGTTAACNCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTNCTCTTTGTTCATTAGCTGGATNTCAGTGTTATGCTTGGNTCCACTCGGCGTGATAAATTATCNATCGCTNAGGATACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATG +>64529_SRR5572438|r|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAAATCATTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACGCCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATG +>100491_SRR5572438|r|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAGATCTTTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATG +>103686_SRR5572438|r|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAAATCATTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGGCTTGGACAATACCTTTATG
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test_01_not_detected.fasta Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,124 @@ +>2150_SRR5572429|bfr +ACAGTCTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACACAGTC +>4821_SRR5572429|bfr +ACAGTCTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGNTCCCGCCTGACCNGGGGTCGCGTTAAAGACTNTGGGTCATCGAGAGCATTTGNACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCNAGACGCTCCNAGGCNCCCTCAGCTCAGATTTAGNCAAACCGCGTGCGGTAAACACGGGAGANCAGCTTCCNTCCGGTANCCGCAANGGAAGGGGACGACGATTTGNGACACCCAGGGAGACGTGCCCTCAGNCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGATTCGATGACTCACACAGTC +>10097_SRR5572430|r +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAGGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>16217_SRR5572430|bfr +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGATTCACAGCTA +>24991_SRR5572430|r +TAGCTGTGAATCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>37210_SRR5572430|r +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGACCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>38583_SRR5572430|r +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGATTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTCTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCAACCGCTGAGTTTTAGCATATCAATAAGCGGAGGATAGCT +>40166_SRR5572430|r +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>55631_SRR5572430|bfr +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCTGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGATTCGATGATTCACAGCTA +>67029_SRR5572430|r +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>72280_SRR5572430|r +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGGCATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>82941_SRR5572430|bfr +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACAGCTA +>110667_SRR5572430|r +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGGCGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>116764_SRR5572430|bfr +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCTCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCACTTGGACAGGNGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGAGGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGNAGGCTTGGGGCGCAACTTGCGTGCAAAGAATCGATGACTCACAGCTA +>119907_SRR5572430|bfr +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACAGCTA +>133359_SRR5572430|bfr +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAGGGCTTGGGGCGCAACTTGTGTTCAAAGACTCGCTGACTCACAGCTA +>152008_SRR5572430|bfr +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACAGCTA +>8277_SRR5572431|r +GTCGCGTGAGTCATCGAGTCTTTGAACGCAAGTTGCNCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGNCGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGNNGGTGAAACAAANCCTCGTCATTTTGTCGGTCGCTCNTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGNCNGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGTCGC +>11225_SRR5572431|bfr +GCGACTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACGCGAC +>16084_SRR5572431|r +GTCGCGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAACGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGTCGC +>32756_SRR5572431|bfr +GCGACTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCANCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCANAGGATGGGGACGNCGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGGCTCACGCGAC +>38016_SRR5572431|bfr +GCGACTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTACCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACGCGAC +>57950_SRR5572431|r +GTCGCGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCAGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGTCGC +>23021_SRR5572432|r +CGTCGAGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACGTCGA +>26380_SRR5572432|r +CGTCGAGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGATGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTNCATATGCTCTCGATGACACAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACGTCGA +>52798_SRR5572432|fr +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAGGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGAGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGNCGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>57614_SRR5572432|fr +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>58620_SRR5572432|r +CGTCGAGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACGTCGA +>60476_SRR5572432|r +CGTCGAGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACGTCGA +>63048_SRR5572432|r +CGTCGAGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTCTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACGTCGA +>69944_SRR5572432|r +CGTCGAGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGGCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACGTCGA +>77272_SRR5572432|fr +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACNATTTGTGACACACAGGCAGACGTGCCCTCNGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>80421_SRR5572432|fr +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>88221_SRR5572432|fr +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGATTCACTCGACG +>96939_SRR5572432|fr +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>99283_SRR5572432|fr +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGAGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>107559_SRR5572432|fr +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>18259_SRR5572433|r +GTCGCGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTTTCCAAATGCTCTCGATGACTCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGTCGC +>5314_SRR5572435|fr +ACTGCTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCNCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCNCCACCNCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCAATGATTCACACTGC +>21163_SRR5572435|r +GCAGTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGCAGT +>23380_SRR5572435|r +GCAGTGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGCAGT +>28473_SRR5572435|fr +ACTGCTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACACTGC +>44904_SRR5572435|r +GCAGTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGGGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGCAGT +>83509_SRR5572435|r +GCAGTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTATCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCNCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAGCAGT +>10044_SRR5572436|bfr +CATGCGGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAGAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>27083_SRR5572436|bfr +CATGCGGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>30856_SRR5572436|bfr +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCTAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACCGCATG +>31515_SRR5572436|bfr +CATGCGGTGAGTCATCGAGTCTTTGAACACAAGTTGCGCCCCAAGCCTTCTGGCTGGGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>39390_SRR5572436|bfr +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCCGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGNCGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGANGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCNCAGCCAGAAGGCTTGNNGCGCAACNTGCGTTCAAAGACTCGATAATTCACCGCATG +>43887_SRR5572436|bfr +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGCGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGATTCGATGACTCACCGCATG +>49165_SRR5572436|bfr +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACCGCATG +>56974_SRR5572436|bfr +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGNTCCCGCCTGACCNGGGGTCGCGTTAAAGACTTTGNGNNATNGAGAGCATTTGGACAGGAGCGACCGACANAANNNCNAGGCTTTGTTTCACCACCGCANGTCGAAACGCTCNAANGCGTCCTCAGCTNAGATNTAGGCCAACCGCGTGCGGNNACACACGGGAGACCAGCTTCCGTCCAGTANCCGCAAAGGATGGGGACGACGNTTTGTGACAGCCAGGCGGNCGTGCCCTCANCCAGAAGGNTTNGGGCGCANCTTGCATTCAAGGACTCGATGACTCACCGCATG +>59309_SRR5572436|bfr +CATGCGGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>60649_SRR5572436|bfr +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTACTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACCGCATG +>63598_SRR5572436|bfr +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACTTGGGGTCGCGTTAAAGACTTTGNGTCATCGAGAGCATTTGGACAGGNGCGACCGACAAAATGACGNGGCTTTGTTTCACCACNGCATGTCGAGACGCTCCAAGGTGTCCNCAGCNCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGAACAGCNTCCGNCNGGTATCCNCAAAGGAGGGGGACGACGANTTGTGANACCCAGGCAGACGTGCCCTCAGCCAGAAAGCTTGGGGCGCAACNTGCGTTCAAAGACTCGATGATTCACCGCATG +>78636_SRR5572436|bfr +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGATTCGATGACTCACCGCATG +>90730_SRR5572436|bfr +CATGCGGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAAGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCCCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>118563_SRR5572436|bfr +CATGCGGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>133834_SRR5572436|bfr +CATGCGGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGNCTGAGNGCACGTCTGCCTGGGTTTCACAAATCGTCGTCCCCATCCTTTTCGGATACCGGACGGAAGCTGNNCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGANATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATNACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>54859_SRR5572438|bfr +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGATTCGATGATTCACAGCTA +>95460_SRR5572438|r +TAGCTGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGACTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCCCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>97519_SRR5572438|bfr +AGCTATCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACATGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGAGACGACGATTTGTGACACCCAGGCAGACGTGCCCTCAGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGATTCGATGACTCACAGCTA
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test_01_not_detected.txt Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,62 @@ +2150_SRR5572429|bfr +4821_SRR5572429|bfr +10097_SRR5572430|r +16217_SRR5572430|bfr +24991_SRR5572430|r +37210_SRR5572430|r +38583_SRR5572430|r +40166_SRR5572430|r +55631_SRR5572430|bfr +67029_SRR5572430|r +72280_SRR5572430|r +82941_SRR5572430|bfr +110667_SRR5572430|r +116764_SRR5572430|bfr +119907_SRR5572430|bfr +133359_SRR5572430|bfr +152008_SRR5572430|bfr +8277_SRR5572431|r +11225_SRR5572431|bfr +16084_SRR5572431|r +32756_SRR5572431|bfr +38016_SRR5572431|bfr +57950_SRR5572431|r +23021_SRR5572432|r +26380_SRR5572432|r +52798_SRR5572432|fr +57614_SRR5572432|fr +58620_SRR5572432|r +60476_SRR5572432|r +63048_SRR5572432|r +69944_SRR5572432|r +77272_SRR5572432|fr +80421_SRR5572432|fr +88221_SRR5572432|fr +96939_SRR5572432|fr +99283_SRR5572432|fr +107559_SRR5572432|fr +18259_SRR5572433|r +5314_SRR5572435|fr +21163_SRR5572435|r +23380_SRR5572435|r +28473_SRR5572435|fr +44904_SRR5572435|r +83509_SRR5572435|r +10044_SRR5572436|bfr +27083_SRR5572436|bfr +30856_SRR5572436|bfr +31515_SRR5572436|bfr +39390_SRR5572436|bfr +43887_SRR5572436|bfr +49165_SRR5572436|bfr +56974_SRR5572436|bfr +59309_SRR5572436|bfr +60649_SRR5572436|bfr +63598_SRR5572436|bfr +78636_SRR5572436|bfr +90730_SRR5572436|bfr +118563_SRR5572436|bfr +133834_SRR5572436|bfr +54859_SRR5572438|bfr +95460_SRR5572438|r +97519_SRR5572438|bfr
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test_01_problematic.tabular Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,38 @@ +7808_SRR5572429|bfr End of SSU sequence not found; Start of 5.8S sequence not found +17841_SRR5572429|r End of SSU sequence not found; Start of 5.8S sequence not found +10297_SRR5572430|r End of SSU sequence not found; Start of 5.8S sequence not found +70284_SRR5572430|bfr End of SSU sequence not found; Start of 5.8S sequence not found +151248_SRR5572430|r End of SSU sequence not found; Start of 5.8S sequence not found +155395_SRR5572430|r End of SSU sequence not found; Start of 5.8S sequence not found +53558_SRR5572432|fr End of SSU sequence not found; Start of 5.8S sequence not found +73213_SRR5572432|r End of SSU sequence not found; Start of 5.8S sequence not found +74880_SRR5572432|fr End of SSU sequence not found; Start of 5.8S sequence not found +100926_SRR5572432|fr End of SSU sequence not found; Start of 5.8S sequence not found +18907_SRR5572433|r End of SSU sequence not found; Start of 5.8S sequence not found +33225_SRR5572433|bfr End of SSU sequence not found; Start of 5.8S sequence not found +36440_SRR5572433|bfr End of SSU sequence not found; Start of 5.8S sequence not found +42477_SRR5572433|r End of SSU sequence not found; Start of 5.8S sequence not found +50746_SRR5572433|r End of SSU sequence not found; Start of 5.8S sequence not found +10088_SRR5572434|r End of SSU sequence not found; Start of 5.8S sequence not found +10939_SRR5572434|r End of SSU sequence not found; Start of 5.8S sequence not found +19705_SRR5572434|fr End of SSU sequence not found; Start of 5.8S sequence not found +25963_SRR5572434|r End of SSU sequence not found; Start of 5.8S sequence not found +60502_SRR5572434|r End of SSU sequence not found; Start of 5.8S sequence not found +79692_SRR5572436|bfr End of SSU sequence not found; Start of 5.8S sequence not found +5749_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +6609_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +23055_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +27631_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +30780_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +33944_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +36405_SRR5572437|r End of SSU sequence not found; Start of 5.8S sequence not found +37680_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +45410_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +56304_SRR5572437|r End of SSU sequence not found; Start of 5.8S sequence not found +58773_SRR5572437|r End of SSU sequence not found; Start of 5.8S sequence not found +65449_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +26515_SRR5572438|r End of SSU sequence not found; Start of 5.8S sequence not found +37564_SRR5572438|bfr End of SSU sequence not found; Start of 5.8S sequence not found +64529_SRR5572438|r End of SSU sequence not found; Start of 5.8S sequence not found +100491_SRR5572438|r End of SSU sequence not found; Start of 5.8S sequence not found +103686_SRR5572438|r End of SSU sequence not found; Start of 5.8S sequence not found
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test_01_summary.txt Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,29 @@ +ITSx run started at Fri Apr 29 18:55:04 2022. +----------------------------------------------------------------- +Number of sequences in input file: 100 +Sequences detected as ITS by ITSx: 38 + On main strand: 21 + On complementary strand: 17 +Sequences detected as chimeric by ITSx: 0 +ITS sequences by preliminary origin: + Alveolates: 0 + Amoebozoa: 0 + Bacillariophyta: 0 + Brown algae: 0 + Bryophytes: 0 + Euglenozoa: 0 + Eustigmatophytes: 0 + Fungi: 38 + Green algae: 0 + Liverworts: 0 + Metazoa: 0 + Microsporidia: 0 + Oomycetes: 0 + Prymnesiophytes: 0 + Raphidophytes: 0 + Red algae: 0 + Rhizaria: 0 + Synurophyceae: 0 + Tracheophyta: 0 +----------------------------------------------------------------- +ITSx run finished at Fri Apr 29 18:55:16 2022.
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test_02_found.tabular Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,92 @@ +2150_SRR5572429|bfr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +7808_SRR5572429|bfr 375 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-309 LSU: 310-375 Broken or partial sequence, only partial 5.8S! +17841_SRR5572429|r 323 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-257 LSU: 258-323 Broken or partial sequence, only partial 5.8S! +10297_SRR5572430|r 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +16217_SRR5572430|bfr 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +24991_SRR5572430|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +37210_SRR5572430|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +38583_SRR5572430|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +40166_SRR5572430|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +55631_SRR5572430|bfr 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +67029_SRR5572430|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +70284_SRR5572430|bfr 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +72280_SRR5572430|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +82941_SRR5572430|bfr 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +110667_SRR5572430|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +116764_SRR5572430|bfr 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +119907_SRR5572430|bfr 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +133359_SRR5572430|bfr 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +151248_SRR5572430|r 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +152008_SRR5572430|bfr 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +155395_SRR5572430|r 377 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-312 LSU: 313-377 Broken or partial sequence, only partial 5.8S! +8277_SRR5572431|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +11225_SRR5572431|bfr 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +16084_SRR5572431|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +32756_SRR5572431|bfr 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +38016_SRR5572431|bfr 328 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-263 LSU: 264-328 Broken or partial sequence, only partial 5.8S! +57950_SRR5572431|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +23021_SRR5572432|r 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +26380_SRR5572432|r 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +52798_SRR5572432|fr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +53558_SRR5572432|fr 497 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-431 LSU: 432-497 Broken or partial sequence, only partial 5.8S! +57614_SRR5572432|fr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +58620_SRR5572432|r 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +60476_SRR5572432|r 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +63048_SRR5572432|r 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +73213_SRR5572432|r 320 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-254 LSU: 255-320 Broken or partial sequence, only partial 5.8S! +74880_SRR5572432|fr 365 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-299 LSU: 300-365 Broken or partial sequence, only partial 5.8S! +80421_SRR5572432|fr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +88221_SRR5572432|fr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +96939_SRR5572432|fr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +99283_SRR5572432|fr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +100926_SRR5572432|fr 358 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-292 LSU: 293-358 Broken or partial sequence, only partial 5.8S! +107559_SRR5572432|fr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +18259_SRR5572433|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +18907_SRR5572433|r 391 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-326 LSU: 327-391 Broken or partial sequence, only partial 5.8S! +33225_SRR5572433|bfr 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +36440_SRR5572433|bfr 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +42477_SRR5572433|r 399 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-334 LSU: 335-399 Broken or partial sequence, only partial 5.8S! +50746_SRR5572433|r 290 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-225 LSU: 226-290 Broken or partial sequence, only partial 5.8S! +10088_SRR5572434|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-263 LSU: 264-329 Broken or partial sequence, only partial 5.8S! +10939_SRR5572434|r 396 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-330 LSU: 331-396 Broken or partial sequence, only partial 5.8S! +19705_SRR5572434|fr 303 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-237 LSU: 238-303 Broken or partial sequence, only partial 5.8S! +25963_SRR5572434|r 318 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-252 LSU: 253-318 Broken or partial sequence, only partial 5.8S! +60502_SRR5572434|r 287 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-221 LSU: 222-287 Broken or partial sequence, only partial 5.8S! +5314_SRR5572435|fr 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +21163_SRR5572435|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +23380_SRR5572435|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +28473_SRR5572435|fr 326 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-261 LSU: 262-326 Broken or partial sequence, only partial 5.8S! +44904_SRR5572435|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +83509_SRR5572435|r 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +10044_SRR5572436|bfr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +27083_SRR5572436|bfr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +30856_SRR5572436|bfr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +31515_SRR5572436|bfr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +39390_SRR5572436|bfr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +43887_SRR5572436|bfr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +49165_SRR5572436|bfr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +59309_SRR5572436|bfr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +60649_SRR5572436|bfr 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-263 LSU: 264-329 Broken or partial sequence, only partial 5.8S! +78636_SRR5572436|bfr 330 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-264 LSU: 265-330 Broken or partial sequence, only partial 5.8S! +79692_SRR5572436|bfr 374 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-308 LSU: 309-374 Broken or partial sequence, only partial 5.8S! +90730_SRR5572436|bfr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +118563_SRR5572436|bfr 331 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-265 LSU: 266-331 Broken or partial sequence, only partial 5.8S! +5749_SRR5572437|bfr 388 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-322 LSU: 323-388 Broken or partial sequence, only partial 5.8S! +6609_SRR5572437|bfr 301 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-234 LSU: 235-301 Broken or partial sequence, only partial 5.8S! +23055_SRR5572437|bfr 334 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-268 LSU: 269-334 Broken or partial sequence, only partial 5.8S! +27631_SRR5572437|bfr 338 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-272 LSU: 273-338 Broken or partial sequence, only partial 5.8S! +30780_SRR5572437|bfr 293 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-227 LSU: 228-293 Broken or partial sequence, only partial 5.8S! +33944_SRR5572437|bfr 286 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-220 LSU: 221-286 Broken or partial sequence, only partial 5.8S! +36405_SRR5572437|r 398 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-332 LSU: 333-398 Broken or partial sequence, only partial 5.8S! +37680_SRR5572437|bfr 300 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-227 LSU: 228-300 Broken or partial sequence, only partial 5.8S! +45410_SRR5572437|bfr 300 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 90-234 LSU: 235-300 Broken or partial sequence, only partial 5.8S! +56304_SRR5572437|r 299 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-233 LSU: 234-299 Broken or partial sequence, only partial 5.8S! +58773_SRR5572437|r 293 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-227 LSU: 228-293 Broken or partial sequence, only partial 5.8S! +65449_SRR5572437|bfr 301 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 81-227 LSU: 228-301 Broken or partial sequence, only partial 5.8S! +26515_SRR5572438|r 340 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-275 LSU: 276-340 Broken or partial sequence, only partial 5.8S! +37564_SRR5572438|bfr 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 80-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +54859_SRR5572438|bfr 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +64529_SRR5572438|r 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +97519_SRR5572438|bfr 329 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 78-264 LSU: 265-329 Broken or partial sequence, only partial 5.8S! +100491_SRR5572438|r 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S! +103686_SRR5572438|r 372 bp. SSU: Not found ITS1: Not found 5.8S: No start ITS2: 79-307 LSU: 308-372 Broken or partial sequence, only partial 5.8S!
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test_02_its2.fasta Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,184 @@ +>2150_SRR5572429|bfr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>7808_SRR5572429|bfr|F|ITS2 Extracted ITS2 sequence 80-309 (230 bp) +AATTCTTCAAAGTAAATCTTTTGTTAATTCAATTGGTTTCACTTTGGTATTGGAGGTCCTTTGCAGCTTCACACCTGCTCCTCTTTGTGCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCTCTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCCAATAGTCCATTGGCTTGGACAATACCTTTATG +>17841_SRR5572429|r|F|ITS2 Extracted ITS2 sequence 81-257 (177 bp) +ACACCCCACATTCATAATTTTGTTGTGAATGGAAATGAGAGTTTCGGCTTTATTGCTGAATTCTTTAAAATTATTAGGCCTGAACTATTGTTCTTTCTGCCTGAACATTTTTTTAATATAAAGGAATGCTCTAGTAAAAAGACTATCTCTGGGGCCTCCCAAATAAATCATTCTTAA +>10297_SRR5572430|r|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAAATCATTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTGTGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATG +>16217_SRR5572430|bfr|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>24991_SRR5572430|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>37210_SRR5572430|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGACCAAATGCTCTCGATGACCCAAAGTCTTTA +>38583_SRR5572430|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGATTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTCTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>40166_SRR5572430|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>55631_SRR5572430|bfr|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACAGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>67029_SRR5572430|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>70284_SRR5572430|bfr|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAAATCATTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATG +>72280_SRR5572430|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGGCATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>82941_SRR5572430|bfr|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>110667_SRR5572430|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGGCGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>116764_SRR5572430|bfr|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCCTCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCNCCTGTCCAAGTGCTCTCGATGACCCAAAGTCTTTA +>119907_SRR5572430|bfr|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>133359_SRR5572430|bfr|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>151248_SRR5572430|r|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAAATCTTTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCTTTGACTTGGACAATACCTTTATG +>152008_SRR5572430|bfr|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>155395_SRR5572430|r|F|ITS2 Extracted ITS2 sequence 79-312 (234 bp) +AAATTCTCAAAGTAAACCTTTTGTTAATTCAACTGGTCTTTGCTTTGGATTTGGAGGCCTCTGCAGATGTCACAGTCTGCTCCTCTTAAATAAATTAGCTGGATCTCAGTATACACTTGGTTCCACTCGGCGTGATAAGTATCACTCGCTGAGGACACTGTTAAAAAGGTGGCCAGGCAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAAATTAAAAATTTATG +>8277_SRR5572431|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGNCGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGNNGGTGAAACAAANCCTCGTCATTTTGTCGGTCGCTCNTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>11225_SRR5572431|bfr|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>16084_SRR5572431|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAACGCTCTCGATGACCCAAAGTCTTTA +>32756_SRR5572431|bfr|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGNCGTCCCCATCCTNTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGNTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>38016_SRR5572431|bfr|F|ITS2 Extracted ITS2 sequence 78-263 (186 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGGTACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>57950_SRR5572431|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>23021_SRR5572432|r|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>26380_SRR5572432|r|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGATGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTNCATATGCTCTCGATGACACAAAGTCTTTA +>52798_SRR5572432|fr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGNCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCTCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCCTTA +>53558_SRR5572432|fr|F|ITS2 Extracted ITS2 sequence 81-431 (351 bp) +TCAACCACACCTCGGCGTCATCTCTTCATTGCGACTGCGCATCTGGAGATTGAGTCGGTCACCGAGTTAACTCTTTGGTGTCCCTTCAAATGTGGCAAGGCTTCAGTGTATTGCTCTTCGCGGACAGTATTCCGATTGTACTCGCTAGCGACGTGGATACTCGATCTCTTTTGGTCTTGAATCCAATACGTGGTTAGTGTTTAGGAAAGGAGTATTGCGCCGTGTGCACCGTCTGTGAGTCCTCTCTTTTGAGGACAAACAGGCTGATTCGCTTGTTTTTGATTTGCGTTTGTGCGCCTCGTGTGTACAGACTGCCGATCAAATCCACACACTTGTGTGTGCTGTTTTCGA +>57614_SRR5572432|fr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>58620_SRR5572432|r|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>60476_SRR5572432|r|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>63048_SRR5572432|r|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTCTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>73213_SRR5572432|r|F|ITS2 Extracted ITS2 sequence 81-254 (174 bp) +CTACCCTCAGGCCCAGCCGCTTAGGCGGTTCGGGTGCTGGTGTTGGGGCATGCAGGCCACTGCAGCCTCTAAATTCATCGGCGTACTGGCGTACGTTGCCCTTTGCGTAGTGGATTTTTCTTTTCTCGCATCGGGCCGTGCCTCGTCCGGCCGCTAACTTTTTTTACTTTTTTA +>74880_SRR5572432|fr|F|ITS2 Extracted ITS2 sequence 81-299 (219 bp) +AAACCCTCAACCCTAGATTGGTTAAAACCTTTCTTTGGTTTGGATTTGGACGTTTGTCGATGATAAGTCGGCTCGTCTTAAAAGTAATAGCTGGATCTGTCTCGCGACATGGTTTGACTTGGCGTAATAAGNATTTCGCTAAGGACATCTTCGGATGGCCGCGTTGCAGGACTAAAGACCGCTTTCTAATCCATTGATCTTACGATTAATATTCTTGAC +>80421_SRR5572432|fr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>88221_SRR5572432|fr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>96939_SRR5572432|fr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>99283_SRR5572432|fr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCTCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>100926_SRR5572432|fr|F|ITS2 Extracted ITS2 sequence 81-292 (212 bp) +AATTCTCAACCCCTCCAGCTTTTGCGAGCTGCGATGGGGCTTGGATGTTGGGAGTTTGCGGGCCTTTATTAATAGAGGTCGGCTCTCCTAAAATGCATTAGCGGAACCGTTTGCAGTCCCGTCACTAGTGTGATAATTATCTACACTGTGACGATTGCTCTCTGACTAGTNCAGCTGCTAATCGTCCCCTGTCGGACAATGTTTAATGAACT +>107559_SRR5572432|fr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>18259_SRR5572433|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTTTCCAAATGCTCTCGATGACTCAAAGTCTTTA +>18907_SRR5572433|r|F|ITS2 Extracted ITS2 sequence 80-326 (247 bp) +AACACCTCAAAGCTTTTGATTTTTTTTGAAAGCTTTGGACTTGAGCAATCCCAACACCAGTCTTTTTTAGATCGGAGGCGGGTTGCTTGAAATGCAGGTGCAGCTGGACATTCTCCTGAGCTAAAAGCATATTCATTTAGTCCCGTCAAACGGATTATTACTTTCGCTGCAGCTAACATAAAGGGAGTTTGACCGTATTGGCTGACTGATGCAGGATTTCACAGGGGTCGGCAACGACGCTTGTTAA +>33225_SRR5572433|bfr|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAAATCTTTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCAATCGCTGAGGACACNGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATG +>36440_SRR5572433|bfr|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAAATCTTTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATG +>42477_SRR5572433|r|F|ITS2 Extracted ITS2 sequence 80-334 (255 bp) +AACACCTCAACTCCCTCTTTTCTTTNTTGAAATGAGGGNGCTGGACTTGAGTGATCCCAACACTTTTCTTCACTGAAAAGTGGCGGGTCACTTGAAATGCAGGTGCAGCTGGACTTTTCTCTGAGCTATAAGCATATCTATTTAGTCTGCCTAAAAAACAGATTATTACCTTTGCTGCAGCTAACATAAAGGAGATGAGTTCTTGTGCTGACTGATGCAGGATTCACAGAGACAGCTTCGCGGCTGACTTCGTAA +>50746_SRR5572433|r|F|ITS2 Extracted ITS2 sequence 80-225 (146 bp) +TCACCCCTCAAGCCTAGCTTGGTGTTGAGGCCTGCTGTCAAGGCAGCCTCTAAAATCAGTGGCAGTGCTGTCAGGCTCTAAGCGTAGTAAAATAAATCGCTATAGGGTCCTGGTGGATACTCGTCAGAACCCCCCCATTTTTTAAT +>10088_SRR5572434|r|F|ITS2 Extracted ITS2 sequence 81-263 (183 bp) +CACCCCTCGAACCACTATTCGCGTAGCTGGATCGGTGTTGGGGCACTACGGTGCAATACCCGTAGGCCCCGAAATGAAGTGGCGGTCCCGCCACGGCGCCCCCATGCGTAGTAACTTTCAGTCTCGCATCGGGTCCCGGCGGAGGCCTGCCGTCAAGCCTATTTTATAATATCTTTACAGATG +>10939_SRR5572434|r|F|ITS2 Extracted ITS2 sequence 81-330 (250 bp) +AACACCTCAACTCCCTTTTCTTTTTTGAAATGGGAGCTGGACTTGAGTGATCCCAACACTTTTCTCACTGGAAAGTGGCGGGTCACTTGAAATGCAGGTGCAGCTGGACTTTTCTCTGAGCTATAAGCATATCTATTTAGTCTGCCTAAAAAACAGATTATTACCTTTGCTGCAGCTAACATAAAGGAGATGAGTTCCTGTGCTGACCGATGCAGGATTCACAGAGACAGCTTCGCGGCTGACTTTGTAA +>19705_SRR5572434|fr|F|ITS2 Extracted ITS2 sequence 81-237 (157 bp) +CACCTCTCAAGCACAGCTTGGTGTTGGGCGTCTGTCCCTGCGTTGCGCAGTGGACTCGCCTGAAAAGCATCGGCGTCCGGCGGTTGGTCTTGCGCGTAGCAGATTGTTCGCACAAGAACCAGGCCGCAGGGCTGTCGCACACGTCTTTTGATTCTCA +>25963_SRR5572434|r|F|ITS2 Extracted ITS2 sequence 81-252 (172 bp) +TCACCACTCAAGCCCCCGGCTTGGTGTTGGACGGTTTGGTTTAGGTACCCCTAGACCCCTCCTAAGGACAATGACGGCGGCTGTTGAACCCCCGGTACACTGAGCTTCATAACTGAGCACGTATCGGATCTAGGGTTTGGCGGCACCCGGTCTATACCTTTTTTGCTTACAA +>60502_SRR5572434|r|F|ITS2 Extracted ITS2 sequence 81-221 (141 bp) +TAACCCTCAAGCCTAGCTTGGTGTTGGAGCATGCTATCTAGCAGCTCTTAAAATCAGTGGCAGTGCCTTCCGGCTCTAAGCGTAGTAATTCTTCTCGCTATAGAAACCGGTTGGTTCGCGCCAGAACCCCCAATTTTCTAT +>5314_SRR5572435|fr|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGNGGTGGNGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>21163_SRR5572435|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>23380_SRR5572435|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>28473_SRR5572435|fr|F|ITS2 Extracted ITS2 sequence 78-261 (184 bp) +ACAAATCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>44904_SRR5572435|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGGGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>83509_SRR5572435|r|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTATCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCNCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>10044_SRR5572436|bfr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAGAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>27083_SRR5572436|bfr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>30856_SRR5572436|bfr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTAGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>31515_SRR5572436|bfr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>39390_SRR5572436|bfr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCNTCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGNCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>43887_SRR5572436|bfr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +GCAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>49165_SRR5572436|bfr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>59309_SRR5572436|bfr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>60649_SRR5572436|bfr|F|ITS2 Extracted ITS2 sequence 79-263 (185 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGTACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCGATGACCCAAAGTCTTTA +>78636_SRR5572436|bfr|F|ITS2 Extracted ITS2 sequence 79-264 (186 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>79692_SRR5572436|bfr|F|ITS2 Extracted ITS2 sequence 80-308 (229 bp) +AATTCTTCAAAGTAAATCTTTTGTTAATTCAATTGGTTTCACTTTGGTATCGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTGCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCCAATAGTCCATTGACTTGGACAATACCTTTATG +>90730_SRR5572436|bfr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAAGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCCCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>118563_SRR5572436|bfr|F|ITS2 Extracted ITS2 sequence 79-265 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>5749_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 81-322 (242 bp) +AACACCTCAACTCCCTTTCTTCTTGAAATGGAGCTGGACTTGAGTGATCCCAACGCTTTATTCATAAAGTGGCGGGTTACTTGAAATGCAGGTGCAGCTGGACTTTTCTCTGAGCTATAAGCATATCTATTTAGTCTGCCTCAAAAACAGATTATTACCTTTGCTGCAGCTAACATAAAGGAGATTAGTTCTTGTGCTGACTGATGCAGGATTCACAAAGACCGCTTCGGCTGTCTTTGTAA +>6609_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 81-234 (154 bp) +GTNCCTTCAAGCTCTGCTTGNTGTTGGGNGTTTNTCTCGCCTTGGNGTGTAGACTCGCCNCAAACCAATNGGCGGCCGGCGTATTGATTTCGGNGCGCAGTACATCTCGCGCTTTGCACTCATAACGACGACGTCCAAAAGTACATTTTTACAC +>23055_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 81-268 (188 bp) +AACTTCTCAACCACTTGATTTTTTTTGGGTGGCTTGGATGTGGGGGGTTGTTTGCAGGCTTTTCTTGAGGTCAGCTCCCCTGAAATGTATTAGTAGTATCTGAGGCTATTACTATAGGTGTGATAAATTATCTATGCCTTTGGTATGCTGCATGAACTGCTTCCAACAAAGATAAAACCTTTTGATGA +>27631_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 80-272 (193 bp) +AAACCCCTCGAGTCCTCTGGCTTTAACCGGCCACAGGAGCCTCGGTGTTGCTCGTGGAGGTACCTCTGGTTCCTCCTGGGCGAATTCCATTGTGCTGATCCACAGGTCGCCAAAGCGTAGTAAGCCTAAAAAGCTCTCGCCTGGCGCAAGACCTTGCTGGTATCATGCCGTTGAACCCCATAACCCATCAAGA +>30780_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 81-227 (147 bp) +AAACCTTCAAGCTCTGCTTGGTGTTGGGTGTTTGTTCCGCCTCAGCGTGTGGACTCGCCTTAAATTCATTGGCAGCCGGTATGTTGGCTTCGTGCGCAGCACATTGCAAGCGATTTCAACAGACCCCCTTCCACAAGCCTTTTTTTA +>33944_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 81-220 (140 bp) +CAACCCTCAAGCTCTGCTTGGTGTTGGGCCCCGCCCGCTCGCGGCCGGCCCTAAAGACAGTGGCGGCAGCGTCTGGCTCCAAGCGTAGTACAATCCTCGCTCTGGTGCTAGGCGGTGGCCTGCCAGAACCCCCCTTCTAT +>36405_SRR5572437|r|F|ITS2 Extracted ITS2 sequence 81-332 (252 bp) +AACACCTCAAAGCCTTTTTTCTTTTTTGAAGAAAGGCTTTGGACTTGAGCAATCCCAACACCAATCTTTTAAAGAGAGGGGGCGGGTTGCTTGAAATGCAGGTGCAGCTGGACATTCTCCTGAGCTAAAAGCATATTCATTTAGTCCCGTCAAACGGATTATTACTTTTGCTGCAGCTAATATAAAGGGAGTTTGACTATTTTGGCTGACTGATGCAGGATTTTCACAAGAGTCTTCAAAACCTCTTGTTAA +>37680_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 81-227 (147 bp) +TCACCCCTCAAGCCTAGCTTGGTGTTGAGGCCTGCTGTCAAGGCAGCCTCTAAAATCAGTGGCAGTGCTGTCAGGCTCTAAGCGTAGTAAAATAAATCGCTATAGGGTCCTGGTGGATACTCGTCAGAACCCCCCATTTTTTTTAAT +>45410_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 90-234 (145 bp) +CAACACTCAAGCTCTGCTTGGTATTGGGCCTTGCCGGCAACGGCAGGCCTTAAAATCAGTGGCGGCGCCATCTGGCTCTAAGCGTAGTAATACCCCTCGCTATAGAGTTCCGGTGGATGCTGGCTAGCAACCCCCAACTTTCTAA +>56304_SRR5572437|r|F|ITS2 Extracted ITS2 sequence 81-233 (153 bp) +GTACCTTCAAGCCTTGCTTGGTGTTGGGTGTTTGTCTCGCCTTTGCGTGTAGACTCGCCTCAAAACAATTGGCAGCCGGCGTATTGATTTCGGAGCGCAGTACATCTCGCGCTTTGCACTCATAACGACGACGTCCAAAAGTACATTTTACAC +>58773_SRR5572437|r|F|ITS2 Extracted ITS2 sequence 81-227 (147 bp) +AAACCTTCAAGCTCTGCTTGGTGTTGGGTGTTTGTTCCGCCTCAGCGTGTGGACTCGCCTTAAATTCATTGGCAGCCGGTATGTTGGCTTCGTGCGCAGCACATTGCAAGCGATTTCAACAGACCCCCTTCCACAAGCCTTTTTTTA +>65449_SRR5572437|bfr|F|ITS2 Extracted ITS2 sequence 81-227 (147 bp) +TCACCCCTCAAGCCTAGCTTGGTGTTGAGGCCTGCTGNCAAGGCAGCCTCTAAAATCAGTGGCAGTGCTGTCAGGCTCTAAGCGTAGTAAAATAAATCGCTATAGGGTCCTGGTGGATACTCGTCAGAACCCCCCCATTTTTTTAAT +>26515_SRR5572438|r|F|ITS2 Extracted ITS2 sequence 80-275 (196 bp) +GCACTCTCACACCTAACCTTTGGGTTATGATGTGGAATTGGGATGCGCCGATTTTTACAAGTCGGCACTCCTAAAATGTAGCTCTTGGCTGTTTCCTACTACAGCAGTTTGGCCTAATAGTTTTGACTTTTGTCAAATCTTTGGCTACATTTGCTTCTGGAAATCAGTCTTGATAATACAGAAAACTCATTTCAAA +>37564_SRR5572438|bfr|F|ITS2 Extracted ITS2 sequence 80-307 (228 bp) +ATTCTTCANAGTAAATCTTTTGTTAACNCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTNCTCTTTGTTCATTAGCTGGATNTCAGTGTTATGCTTGGNTCCACTCGGCGTGATAAATTATCNATCGCTNAGGATACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATG +>54859_SRR5572438|bfr|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>64529_SRR5572438|r|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAAATCATTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACGCCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATG +>97519_SRR5572438|bfr|F|ITS2 Extracted ITS2 sequence 78-264 (187 bp) +ACAAATCGTCGTCTCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTA +>100491_SRR5572438|r|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAGATCTTTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGACTTGGACAATACCTTTATG +>103686_SRR5572438|r|F|ITS2 Extracted ITS2 sequence 79-307 (229 bp) +AATTCTTCAAAGTAAATCATTTGTTAACTCAATTGGTTTCACTTTGGTATTGGAGGTCTTTTGCAGCTTCACACCTGCTCCTCTTTGTTCATTAGCTGGATCTCAGTGTTATGCTTGGTTCCACTCGGCGTGATAAATTATCTATCGCTGAGGACACTGTAAAAGGTGGCCAAGGTAAATGCAGATGAACCGCTTCTAATAGTCCATTGGCTTGGACAATACCTTTATG
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test_02_not_detected.fasta Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,16 @@ +>4821_SRR5572429|bfr +ACAGTCTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGNTCCCGCCTGACCNGGGGTCGCGTTAAAGACTNTGGGTCATCGAGAGCATTTGNACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCNAGACGCTCCNAGGCNCCCTCAGCTCAGATTTAGNCAAACCGCGTGCGGTAAACACGGGAGANCAGCTTCCNTCCGGTANCCGCAANGGAAGGGGACGACGATTTGNGACACCCAGGGAGACGTGCCCTCAGNCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGATTCGATGACTCACACAGTC +>10097_SRR5572430|r +TAGCTGTGAGTCATCGAGTCTTTGAACGCAAGTTGCGCCCCAGGCCTTCTGGCTGAGGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT +>69944_SRR5572432|r +CGTCGAGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGGCTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACGTCGA +>77272_SRR5572432|fr +TCGACGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACCTGGGGTCGCGTTAAAGACTTTGGGTCATCGAGAGCATTTGGACAGGAGCGACCGACAAAATGACGAGGCTTTGTTTCACCACCGCATGTCGAGACGCTCCAAGGCGTCCTCAGCTCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGACCAGCTTCCGTCCGGTATCCGCAAAGGATGGGGACGACNATTTGTGACACACAGGCAGACGTGCCCTCNGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGACTCACTCGACG +>56974_SRR5572436|bfr +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGNTCCCGCCTGACCNGGGGTCGCGTTAAAGACTTTGNGNNATNGAGAGCATTTGGACAGGAGCGACCGACANAANNNCNAGGCTTTGTTTCACCACCGCANGTCGAAACGCTCNAANGCGTCCTCAGCTNAGATNTAGGCCAACCGCGTGCGGNNACACACGGGAGACCAGCTTCCGTCCAGTANCCGCAAAGGATGGGGACGACGNTTTGTGACAGCCAGGCGGNCGTGCCCTCANCCAGAAGGNTTNGGGCGCANCTTGCATTCAAGGACTCGATGACTCACCGCATG +>63598_SRR5572436|bfr +CGCATGTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTGATCCCGCCTGACTTGGGGTCGCGTTAAAGACTTTGNGTCATCGAGAGCATTTGGACAGGNGCGACCGACAAAATGACGNGGCTTTGTTTCACCACNGCATGTCGAGACGCTCCAAGGTGTCCNCAGCNCAGATTTAGGCCAACCGCGTGCGGTAACACACGGGAGAACAGCNTCCGNCNGGTATCCNCAAAGGAGGGGGACGACGANTTGTGANACCCAGGCAGACGTGCCCTCAGCCAGAAAGCTTGGGGCGCAACNTGCGTTCAAAGACTCGATGATTCACCGCATG +>133834_SRR5572436|bfr +CATGCGGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGNCTGAGNGCACGTCTGCCTGGGTTTCACAAATCGTCGTCCCCATCCTTTTCGGATACCGGACGGAAGCTGNNCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGANATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCTCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATNACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGACATGCG +>95460_SRR5572438|r +TAGCTGTGAGTCATCGAATCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCTGAGGGCACGACTGCCTGGGTGTCACAAATCGTCGTCCCCATCCTTTGCGGATACCGGACGGAAGCTGGTCTCCCGTGTGTTACCGCACGCGGTTGGCCTAAATCTGAGCTGAGGACGCCTTGGAGCGTCTCGACATGCGGTGGTGAAACAAAGCCTCGTCATTTTGTCGGTCGCTCCTGTCCAAATGCTCCCGATGACCCAAAGTCTTTAACGCGACCCCAGGTCAGGCGGGATCACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGATAGCT
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test_02_not_detected.txt Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,8 @@ +4821_SRR5572429|bfr +10097_SRR5572430|r +69944_SRR5572432|r +77272_SRR5572432|fr +56974_SRR5572436|bfr +63598_SRR5572436|bfr +133834_SRR5572436|bfr +95460_SRR5572438|r
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test_02_problematic.tabular Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,92 @@ +2150_SRR5572429|bfr End of SSU sequence not found; Start of 5.8S sequence not found +7808_SRR5572429|bfr End of SSU sequence not found; Start of 5.8S sequence not found +17841_SRR5572429|r End of SSU sequence not found; Start of 5.8S sequence not found +10297_SRR5572430|r End of SSU sequence not found; Start of 5.8S sequence not found +16217_SRR5572430|bfr End of SSU sequence not found; Start of 5.8S sequence not found +24991_SRR5572430|r End of SSU sequence not found; Start of 5.8S sequence not found +37210_SRR5572430|r End of SSU sequence not found; Start of 5.8S sequence not found +38583_SRR5572430|r End of SSU sequence not found; Start of 5.8S sequence not found +40166_SRR5572430|r End of SSU sequence not found; Start of 5.8S sequence not found +55631_SRR5572430|bfr End of SSU sequence not found; Start of 5.8S sequence not found +67029_SRR5572430|r End of SSU sequence not found; Start of 5.8S sequence not found +70284_SRR5572430|bfr End of SSU sequence not found; Start of 5.8S sequence not found +72280_SRR5572430|r End of SSU sequence not found; Start of 5.8S sequence not found +82941_SRR5572430|bfr End of SSU sequence not found; Start of 5.8S sequence not found +110667_SRR5572430|r End of SSU sequence not found; Start of 5.8S sequence not found +116764_SRR5572430|bfr End of SSU sequence not found; Start of 5.8S sequence not found +119907_SRR5572430|bfr End of SSU sequence not found; Start of 5.8S sequence not found +133359_SRR5572430|bfr End of SSU sequence not found; Start of 5.8S sequence not found +151248_SRR5572430|r End of SSU sequence not found; Start of 5.8S sequence not found +152008_SRR5572430|bfr End of SSU sequence not found; Start of 5.8S sequence not found +155395_SRR5572430|r End of SSU sequence not found; Start of 5.8S sequence not found +8277_SRR5572431|r End of SSU sequence not found; Start of 5.8S sequence not found +11225_SRR5572431|bfr End of SSU sequence not found; Start of 5.8S sequence not found +16084_SRR5572431|r End of SSU sequence not found; Start of 5.8S sequence not found +32756_SRR5572431|bfr End of SSU sequence not found; Start of 5.8S sequence not found +38016_SRR5572431|bfr End of SSU sequence not found; Start of 5.8S sequence not found +57950_SRR5572431|r End of SSU sequence not found; Start of 5.8S sequence not found +23021_SRR5572432|r End of SSU sequence not found; Start of 5.8S sequence not found +26380_SRR5572432|r End of SSU sequence not found; Start of 5.8S sequence not found +52798_SRR5572432|fr End of SSU sequence not found; Start of 5.8S sequence not found +53558_SRR5572432|fr End of SSU sequence not found; Start of 5.8S sequence not found +57614_SRR5572432|fr End of SSU sequence not found; Start of 5.8S sequence not found +58620_SRR5572432|r End of SSU sequence not found; Start of 5.8S sequence not found +60476_SRR5572432|r End of SSU sequence not found; Start of 5.8S sequence not found +63048_SRR5572432|r End of SSU sequence not found; Start of 5.8S sequence not found +73213_SRR5572432|r End of SSU sequence not found; Start of 5.8S sequence not found +74880_SRR5572432|fr End of SSU sequence not found; Start of 5.8S sequence not found +80421_SRR5572432|fr End of SSU sequence not found; Start of 5.8S sequence not found +88221_SRR5572432|fr End of SSU sequence not found; Start of 5.8S sequence not found +96939_SRR5572432|fr End of SSU sequence not found; Start of 5.8S sequence not found +99283_SRR5572432|fr End of SSU sequence not found; Start of 5.8S sequence not found +100926_SRR5572432|fr End of SSU sequence not found; Start of 5.8S sequence not found +107559_SRR5572432|fr End of SSU sequence not found; Start of 5.8S sequence not found +18259_SRR5572433|r End of SSU sequence not found; Start of 5.8S sequence not found +18907_SRR5572433|r End of SSU sequence not found; Start of 5.8S sequence not found +33225_SRR5572433|bfr End of SSU sequence not found; Start of 5.8S sequence not found +36440_SRR5572433|bfr End of SSU sequence not found; Start of 5.8S sequence not found +42477_SRR5572433|r End of SSU sequence not found; Start of 5.8S sequence not found +50746_SRR5572433|r End of SSU sequence not found; Start of 5.8S sequence not found +10088_SRR5572434|r End of SSU sequence not found; Start of 5.8S sequence not found +10939_SRR5572434|r End of SSU sequence not found; Start of 5.8S sequence not found +19705_SRR5572434|fr End of SSU sequence not found; Start of 5.8S sequence not found +25963_SRR5572434|r End of SSU sequence not found; Start of 5.8S sequence not found +60502_SRR5572434|r End of SSU sequence not found; Start of 5.8S sequence not found +5314_SRR5572435|fr End of SSU sequence not found; Start of 5.8S sequence not found +21163_SRR5572435|r End of SSU sequence not found; Start of 5.8S sequence not found +23380_SRR5572435|r End of SSU sequence not found; Start of 5.8S sequence not found +28473_SRR5572435|fr End of SSU sequence not found; Start of 5.8S sequence not found +44904_SRR5572435|r End of SSU sequence not found; Start of 5.8S sequence not found +83509_SRR5572435|r End of SSU sequence not found; Start of 5.8S sequence not found +10044_SRR5572436|bfr End of SSU sequence not found; Start of 5.8S sequence not found +27083_SRR5572436|bfr End of SSU sequence not found; Start of 5.8S sequence not found +30856_SRR5572436|bfr End of SSU sequence not found; Start of 5.8S sequence not found +31515_SRR5572436|bfr End of SSU sequence not found; Start of 5.8S sequence not found +39390_SRR5572436|bfr End of SSU sequence not found; Start of 5.8S sequence not found +43887_SRR5572436|bfr End of SSU sequence not found; Start of 5.8S sequence not found +49165_SRR5572436|bfr End of SSU sequence not found; Start of 5.8S sequence not found +59309_SRR5572436|bfr End of SSU sequence not found; Start of 5.8S sequence not found +60649_SRR5572436|bfr End of SSU sequence not found; Start of 5.8S sequence not found +78636_SRR5572436|bfr End of SSU sequence not found; Start of 5.8S sequence not found +79692_SRR5572436|bfr End of SSU sequence not found; Start of 5.8S sequence not found +90730_SRR5572436|bfr End of SSU sequence not found; Start of 5.8S sequence not found +118563_SRR5572436|bfr End of SSU sequence not found; Start of 5.8S sequence not found +5749_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +6609_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +23055_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +27631_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +30780_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +33944_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +36405_SRR5572437|r End of SSU sequence not found; Start of 5.8S sequence not found +37680_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +45410_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +56304_SRR5572437|r End of SSU sequence not found; Start of 5.8S sequence not found +58773_SRR5572437|r End of SSU sequence not found; Start of 5.8S sequence not found +65449_SRR5572437|bfr End of SSU sequence not found; Start of 5.8S sequence not found +26515_SRR5572438|r End of SSU sequence not found; Start of 5.8S sequence not found +37564_SRR5572438|bfr End of SSU sequence not found; Start of 5.8S sequence not found +54859_SRR5572438|bfr End of SSU sequence not found; Start of 5.8S sequence not found +64529_SRR5572438|r End of SSU sequence not found; Start of 5.8S sequence not found +97519_SRR5572438|bfr End of SSU sequence not found; Start of 5.8S sequence not found +100491_SRR5572438|r End of SSU sequence not found; Start of 5.8S sequence not found +103686_SRR5572438|r End of SSU sequence not found; Start of 5.8S sequence not found
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test_02_summary.txt Mon May 02 13:03:49 2022 +0000 @@ -0,0 +1,28 @@ +ITSx run started at Fri Apr 29 18:55:28 2022. +----------------------------------------------------------------- +Number of sequences in input file: 100 +Sequences detected as ITS by ITSx: 92 + On main strand: 47 + On complementary strand: 45 +ITS sequences by preliminary origin: + Alveolates: 0 + Amoebozoa: 0 + Bacillariophyta: 0 + Brown algae: 0 + Bryophytes: 0 + Euglenozoa: 0 + Eustigmatophytes: 0 + Fungi: 92 + Green algae: 0 + Liverworts: 0 + Metazoa: 0 + Microsporidia: 0 + Oomycetes: 0 + Prymnesiophytes: 0 + Raphidophytes: 0 + Red algae: 0 + Rhizaria: 0 + Synurophyceae: 0 + Tracheophyta: 0 +----------------------------------------------------------------- +ITSx run finished at Fri Apr 29 18:55:30 2022.