Mercurial > repos > bgruening > rnaz
diff rnaz.xml @ 4:58fd61a8362e draft default tip
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_team/rnaz commit d261ddb93500e1ea309845fa3989c87c6312583d-dirty
author | bgruening |
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date | Wed, 30 Jan 2019 04:13:28 -0500 |
parents | 580ee1e91801 |
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--- a/rnaz.xml Thu Sep 28 11:57:06 2017 -0400 +++ b/rnaz.xml Wed Jan 30 04:13:28 2019 -0500 @@ -1,37 +1,32 @@ <tool id="rnaz" name="RNAz" version="2.1.1"> <description>predicting structurally conserved and thermodynamically stable RNA secondary structures</description> - <requirements> <requirement type="package" version="2.1">rnaz</requirement> </requirements> - <stdio> <exit_code range="1:" level="fatal" description="Error occurred. Please check Tool Standard Error" /> <exit_code range=":-1" level="fatal" description="Error occurred. Please check Tool Standard Error" /> </stdio> - <version_command>RNAz --version</version_command> - <command> -<![CDATA[ - RNAz '$input' - --$forward_or_reverse + <command><![CDATA[ + RNAz + $forward_or_reverse $zscore $locarnate $noshuffle #if $cutoff != -1.0: - --cutoff=$cutoff + --cutoff=$cutoff #end if - > temp.txt && - grep -v -E "^ |^#|^$" temp.txt > '$outfile' && - grep -E "^ |^#|^$" temp.txt -]]> + '$input' + > '$output' + ]]> </command> <inputs> <param format="txt" name="input" type="data" label="Input Alignment File" /> <param name="forward_or_reverse" type="select" label="Scored strand"> - <option value="forward">Score forward strand (-f)</option> - <option value="reverse">Score reverse strand (-r)</option> - <option value="both-strands">Score both strands (-b)</option> + <option value="--forward">Score forward strand (-f)</option> + <option value="--reverse">Score reverse strand (-r)</option> + <option value="--both-strands" selected="true">Score both strands (-b)</option> </param> <param name="zscore" type="select" label="Which type of z-scores"> <option value="--mononucleotide">Use mononucleotide shuffled z-scores</option> @@ -42,20 +37,20 @@ <param argument="--no-shuffle" name="noshuffle" type="boolean" checked="false" truevalue="--no-shuffle" falsevalue="" label="Never fall back to shuffling" /> </inputs> <outputs> - <data name="outfile" format="fasta" /> + <data name="output" format="txt" /> </outputs> <tests> <test> - <param name="input" value="rnaz_input_trna.aln"/> - <output name="outfile" file="rnaz_result_trna.fasta"/> + <param name="input" value="unknown.aln.window"/> + <output name="output" file="unknown.rnaz"/> </test> <test> - <param name="input" value="rnaz_test_input2.aln"/> - <output name="outfile" file="rnaz_result2.fasta"/> + <param name="input" value="tRNA.maf.window"/> + <output name="output" file="tRNA.rnaz"/> </test> </tests> <help> -<![CDATA[ + <![CDATA[ **What it does** @@ -78,36 +73,36 @@ sacCas GCTTCAGTAGCTCAGTCGGAAGAGCGTCAGTCTCATAATCTGAAGGTCGAGAGTTCGAAC - \** * * \** \** \**\** \** \**\** * *\** \**\**\* *\**\* * \**\**\** * +\** * * \** \** \**\** \** \**\** * *\** \**\**\* *\**\* * \**\**\** * **Output** -In Galaxy RNAz gives you 2 output files: a summary file and a result file. For the example input they look like this: +RNAz returns an output file consisting of a summary header section and analyzed seuqence windows. For example they look like this: -Summary: +Header: - Sequences: 4 - Columns: 60 - Reading direction: forward - Mean pairwise identity: 82.50 - Shannon entropy: 0.28395 - G+C content: 0.51667 - Mean single sequence MFE: -16.67 - Consensus MFE: -15.59 - Energy contribution: -15.53 - Covariance contribution: -0.06 - Combinations/Pair: 1.26 - Mean z-score: -0.66 - Structure conservation index: 0.93 - Background model: mononucleotide - Decision model: sequence based alignment quality - SVM decision value: -0.64 - SVM RNA-class probability: 0.238023 - Prediction: OTHER - - -Result file: +Sequences: 4 +Columns: 60 +Reading direction: forward +Mean pairwise identity: 82.50 +Shannon entropy: 0.28395 +G+C content: 0.51667 +Mean single sequence MFE: -16.67 +Consensus MFE: -15.59 +Energy contribution: -15.53 +Covariance contribution: -0.06 +Combinations/Pair: 1.26 +Mean z-score: -0.66 +Structure conservation index: 0.93 +Background model: mononucleotide +Decision model: sequence based alignment quality +SVM decision value: -0.64 +SVM RNA-class probability: 0.238023 +Prediction: OTHER + + +Windows: >sacCer1 @@ -140,16 +135,12 @@ ..((((...((((........))))....(((((((((((....))))))))))))))). (-15.59 = -15.53 + -0.06) - - +Usually this is output serves as input to rnazCluster.pl to reassemble sequences from windows often followed by rnazFilter.pl and rnazSort.pl to select regions of interest. - -]]> + ]]> </help> - <citations> <citation type="doi">10.1142/9789814295291_0009</citation> - </citations> - + </citations> </tool>